Inhibition of HIV protease activity by heterodimer formation.

abstract：:Using solution-state NMR spectroscopy, new insights into the early events governing amelogenin supramolecular self-assembly have been identified using sodium chloride and calcium chloride to trigger the association. Two-dimensional 1H-15N HSQC spectra were recorded for 15N- and 13C-labeled murine amelogenin as a funct...

journal_title：Biochemistry

authors： Buchko GW,Tarasevich BJ,Bekhazi J,Snead ML,Shaw WJ

更新日期：2008-12-16 00:00:00

abstract：:Molecular interactions between the tumor suppressor p53 and the transcriptional coactivators CBP/p300 are critical for the regulation of p53 transactivation and stability. The transactivation domain (TAD) of p53 binds directly to several CBP/p300 domains (TAZ1, TAZ2, NCBD, and KIX). Here we map the interaction between...

journal_title：Biochemistry

authors： Lee CW,Arai M,Martinez-Yamout MA,Dyson HJ,Wright PE

更新日期：2009-03-17 00:00:00

abstract：:Deoxycytidylate (dCMP) hydroxymethylase (CH) catalyzes the formation of 5-(hydroxymethyl)-dCMP, essential for DNA synthesis in phage T4, from dCMP and methylenetetrahydrofolate (CH2THF). The nucleotide analog 5-fluorodeoxuridylate (FdUMP) stoichiometrically inactivates CH by formation of a covalent complex containing ...

journal_title：Biochemistry

authors： Graves KL,Hardy LW

更新日期：1994-11-08 00:00:00

abstract：:The fluorescence polarization intensities from fluorescent probes and the electron paramagnetic resonance spectra from spin probes, specifically modifying elements of a biological assembly such as myosin sulfhydryl 1 (SH1) in muscle fibers, are interpreted in terms of probe order parameters using a model-independent m...

journal_title：Biochemistry

authors： Burghardt TP,Ajtai K

更新日期：1992-01-14 00:00:00

abstract：:The equilibrium binding of influenza virus hemagglutinin to derivatives of its cell-surface ligand, sialic acid, was measured by nuclear magnetic resonance (NMR) spectroscopy. Binding was quantified by observing perturbations of sialic acid resonances in the presence of protein. The major perturbation observed was a c...

journal_title：Biochemistry

authors： Sauter NK,Bednarski MD,Wurzburg BA,Hanson JE,Whitesides GM,Skehel JJ,Wiley DC

更新日期：1989-10-17 00:00:00

abstract：:The folate chromophore in native Escherichia coli DNA photolyase ([6R]-5,10-CH+-H4Pte(Glu)n=3-6) serves as an antenna, transferring light energy to the fully reduced flavin (FADH2) reaction center at high efficiency (EET = 0.92). Apophotolyase reconstituted after an overnight incubation with [6R,S]-5,10-CH+-H4folate (...

journal_title：Biochemistry

authors： Lipman RS,Jorns MS

更新日期：1996-06-18 00:00:00

abstract：:The central importance of chorismate enzymes in bacteria, fungi, parasites, and plants combined with their absence in mammals makes them attractive targets for antimicrobials and herbicides. Two of these enzymes, anthranilate synthase (AS) and aminodeoxychorismate synthase (ADCS), are structurally and mechanistically ...

journal_title：Biochemistry

authors： Culbertson JE,Chung Dh,Ziebart KT,Espiritu E,Toney MD

更新日期：2015-04-14 00:00:00

abstract：:The P22 tailspike endorhamnosidase confers the high specificity of bacteriophage P22 for some serogroups of Salmonella differing only slightly in their O-antigen polysaccharide. We used several biophysical methods to study the binding and hydrolysis of O-antigen fragments of different lengths by P22 tailspike protein....

journal_title：Biochemistry

authors： Baxa U,Weintraub A,Seckler R

更新日期：2020-12-29 00:00:00

abstract：:The active site of water oxidation in Photosystem II (PSII) is a Mn4CaO5 cluster that is located in a cavity between the D1 and CP43 protein subunits by which it is coordinated. The remainder of this cavity is filled with water molecules, which serve as a source of substrate and participate in poorly understood hydrog...

journal_title：Biochemistry

authors： Dilbeck PL,Bao H,Neveu CL,Burnap RL

更新日期：2013-10-01 00:00:00

abstract：:MT1-MMP, a prototypic member of a membrane-type metalloproteinase subfamily, is an invasion promoting protease and an activator of MMP-2. In addition, MT1-MMP proteolysis regulates the functionality of cell-surface adhesion/signaling receptors including tissue transglutaminase (tTG). tTG is known to serve as an adhesi...

journal_title：Biochemistry

authors： Belkin AM,Zemskov EA,Hang J,Akimov SS,Sikora S,Strongin AY

更新日期：2004-09-21 00:00:00

abstract：:In this communication, we present data which describe optimum conditions for reverse transcription of large ribonucleic acid (RNA) templates into deoxyribonucleic acid (DNA) transcripts by the avian retrovirus reverse transcriptase in vitro. In contrast to previous studies, we have optimized all of the reaction compon...

journal_title：Biochemistry

authors： Retzel EF,Collett MS,Faras AJ

更新日期：1980-02-05 00:00:00

abstract：:Simple two-dimensional thin-layer chromatography was found to be useful for the separation of sugar methylated dinucleotides in RNA. Of the 16 possible sequences of the type Nm-Np, 15 were separated and all the sequences were determined. In a mouse hepatoma, MH 134, the levels of the sugar methylation in the 18S and 2...

journal_title：Biochemistry

authors： Hashimoto S,Sakai M,Muramatsu M

更新日期：1975-05-06 00:00:00

abstract：:Taq and T7 DNA polymerases have become basic molecular biology "tools" for DNA sequence analysis. However, Taq, unlike T7 DNA polymerase, is strongly biased against the incorporation of 2',3'-dideoxynucleotide triphosphates (ddNTPs) indicating very different substrate selectivities. Equilibrium binding and rate consta...

journal_title：Biochemistry

authors： Brandis JW,Edwards SG,Johnson KA

更新日期：1996-02-20 00:00:00

abstract：:Catalysis by glycogen phosphorylase involves a mechanism in which binding of one substrate tightens the binding of the other substrate to produce a productive ternary enzyme-substrate complex. In this work the molecular basis for this synergism is probed in crystallographic studies on ternary complexes in which the gl...

journal_title：Biochemistry

authors： Mitchell EP,Withers SG,Ermert P,Vasella AT,Garman EF,Oikonomakos NG,Johnson LN

更新日期：1996-06-11 00:00:00

abstract：:Quorum sensing, the ability of bacteria to sense their own population density through the synthesis and detection of small molecule signals, has received a great deal of attention in recent years. Acyl homoserine lactones (AHLs) are a major class of quorum sensing signaling molecules. In nature, some bacteria that do ...

journal_title：Biochemistry

authors： Chowdhary PK,Keshavan N,Nguyen HQ,Peterson JA,González JE,Haines DC

更新日期：2007-12-18 00:00:00

abstract：:The kringle 2 (K2) module of human plasminogen (Pgn) binds L-lysine and analogous zwitterionic compounds, such as the antifibronolytic agent trans-(aminomethyl)cyclohexanecarboxylic acid (AMCHA). Far-UV CD and NMR spectra reveal little conformational change in K2 upon ligand binding. However, retarded (1)H-(2)H isotop...

journal_title：Biochemistry

authors： Marti DN,Schaller J,Llinás M

更新日期：1999-11-30 00:00:00

abstract：:A method is described for measuring equilibrium constants of DNA-protein interactions using gel chromatography. This technique has been used to study the sequence-specific interaction of the HinfI restriction endonuclease with DNA. HinfI has a monomeric molecular weight of 31000 and exists as a dimer in its active for...

journal_title：Biochemistry

authors： Frankel AD,Ackers GK,Smith HO

更新日期：1985-06-04 00:00:00

abstract：:Inexpensive, straightforward, and rapid medical diagnostics are becoming increasingly important for disease identification in time- and resource-limited settings. Previous attempts to link oligonucleotide-based aptamers and hammerhead ribozymes to form ligand-induced ribozymes have been successful in identifying a var...

journal_title：Biochemistry

authors： Liao AM,Pan W,Benson JC,Wong AD,Rose BJ,Caltagirone GT

更新日期：2018-08-28 00:00:00

abstract：:The complete amino acid sequence of the major component myoglobin from Amazon River dolphin, Inia geoffrensis, was determined by specific cleavage of the protein to obtain large peptides which are readily degraded by the automatic sequencer. Three easily separable peptides were obtained by cleaving the protein with cy...

journal_title：Biochemistry

authors： Dwulet FE,Bogardt RA,Jones BN,Lehman LD,Gurd FR

更新日期：1975-12-02 00:00:00

abstract：:Nucleosome assembly proteins (Naps) influence chromatin dynamics by directly binding to histones. Here we provide a comprehensive structural and biochemical analysis of a Nap protein from Caenorhabditis elegans (CeNap1). CeNap1 naturally lacks the acidic N-terminal tail and has a short C-terminal tail compared to many...

journal_title：Biochemistry

authors： Sarkar P,Zhang N,Bhattacharyya S,Salvador K,D'Arcy S

更新日期：2019-01-15 00:00:00

abstract：:The contact between EcoRI endonuclease and the "primary clamp" phosphate of its recognition site pGAATTC is absolutely required for recognition of the canonical and all variant DNA sites. We have probed this contact using oligonucleotides containing single stereospecific (Rp)- or (Sp)- phosphorothioates (Ps). At the G...

journal_title：Biochemistry

authors： Kurpiewski MR,Koziolkiewicz M,Wilk A,Stec WJ,Jen-Jacobson L

更新日期：1996-07-09 00:00:00

abstract：:The objective of this study was to determine the molecular factors that lead to beta-lactamase inhibitor resistance for the M69V variant in SHV-1 beta-lactamase. With mechanism-based inhibitors, the beta-lactamase forms an acyl-enzyme intermediate that consists of a trans-enamine derivative in the active site. This st...

journal_title：Biochemistry

authors： Totir MA,Padayatti PS,Helfand MS,Carey MP,Bonomo RA,Carey PR,van den Akker F

更新日期：2006-10-03 00:00:00

abstract：:Intrinsically disordered proteins (IDPs) are functionally active despite lacking a well-defined three-dimensional structure. Such proteins often undergo a disorder-to-order transition, or induced folding, when binding to their specific physiological partner. Because of cooperativity, the folding and binding steps typi...

journal_title：Biochemistry

authors： Bonetti D,Troilo F,Toto A,Brunori M,Longhi S,Gianni S

更新日期：2017-07-25 00:00:00

abstract：:T4 polynucleotide kinase is widely used for 5'-phosphorylation of DNA and RNA oligonucleotide termini, but no natural protein enzyme is capable of 3'-phosphorylation. Here, we report the in vitro selection of deoxyribozymes (DNA enzymes) capable of DNA oligonucleotide 3'-phosphorylation, using a 5'-triphosphorylated R...

journal_title：Biochemistry

authors： Camden AJ,Walsh SM,Suk SH,Silverman SK

更新日期：2016-05-10 00:00:00

abstract：:Three electrochromic difference spectra induced by the deposition of (1) a negative charge on the primary quinone acceptor, Q(A), (2) a positive charge on (or near) Tyr161 of the D1 subunit (Y(Z)), and (3) a positive charge on the manganese cluster were determined at room temperature in photosystem II (PSII) core part...

journal_title：Biochemistry

authors： Mulkidjanian AY,Cherepanov DA,Haumann M,Junge W

更新日期：1996-03-05 00:00:00

abstract：:We recently introduced a new substrate rescue tool for investigating enzymatic base flipping by uracil DNA glycosylase (UDG) in which a bulky pyrene nucleotide wedge (Y) was placed opposite a uracil in duplex DNA (i.e., a U/Y pair), thereby preorganizing the target base in an extrahelical conformation [Jiang, Y. L., e...

journal_title：Biochemistry

authors： Jiang YL,Stivers JT,Song F

更新日期：2002-09-17 00:00:00

abstract：:The solvent accessibility of thrombin in its substrate-free and substrate-bound forms has been compared by amide hydrogen/deuterium (H/(2)H) exchange. The optimized inhibitor peptide dPhe-Pro-Arg chloromethyl ketone (PPACK) was used to simulate the substrate-bound form of thrombin. These studies were motivated by the ...

journal_title：Biochemistry

authors： Croy CH,Koeppe JR,Bergqvist S,Komives EA

更新日期：2004-05-11 00:00:00

abstract：:The amidotransferase family of enzymes utilizes the ammonia derived from the hydrolysis of glutamine for a subsequent chemical reaction catalyzed by the same enzyme. The ammonia intermediate does not dissociate into solution during the chemical transformations. A well-characterized example of the structure and mechani...

journal_title：Biochemistry

authors： Raushel FM,Thoden JB,Holden HM

更新日期：1999-06-22 00:00:00

abstract：:Cellular Ca2+ transients and Ca2+-binding proteins regulate physiological phenomena as diverse as muscle contraction, neurosecretion, and cell division. When Ca2+ is rapidly mixed with slow Ca2+ chelators, EGTA, or Mg2+/EDTA, artificial Ca2+ transients (ACTs) of varying duration (0.1-50 ms half-widths (hws)) and ampli...

journal_title：Biochemistry

authors： Davis JP,Tikunova SB,Walsh MP,Johnson JD

更新日期：1999-03-30 00:00:00

abstract：:Many bacteria produce isonitrile-containing natural products that are derived from aromatic amino acids. The synthetic clusters that control biosynthesis most commonly encode two enzymes, designated PvcA and PvcB, as well as additional enzymes that direct synthesis of the natural product. The PvcA enzyme installs the ...

journal_title：Biochemistry

authors： Zhu J,Lippa GM,Gulick AM,Tipton PA

更新日期：2015-04-28 00:00:00