Enzymatic synthesis of deoxyribonucleic acid by the avian retrovirus reverse transcriptase in vitro: optimum conditions required for transcription of large ribonucleic acid templates.

abstract：:The acetoacetate decarboxylase-like superfamily (ADCSF) is a little-explored group of enzymes that may contain new biocatalysts. The low level of sequence identity (∼20%) between many ADCSF enzymes and the confirmed acetoacetate decarboxylases led us to investigate the degree of diversity in the reaction and substrate...

journal_title：Biochemistry

authors： Mydy LS,Hoppe RW,Hagemann TM,Schwabacher AW,Silvaggi NR

更新日期：2019-10-08 00:00:00

abstract：:An improved purification procedure for the alpha 2 beta 2 complex of tryptophan synthase from Escherichia coli has been developed. It consists of DEAE-Sephacel chromatography, followed by hydrophobic chromatography on Sepharose CL 4B, and leads to material with a higher specific activity than reported previously. Inhi...

journal_title：Biochemistry

authors： Tschopp J,Kirschner K

更新日期：1980-09-16 00:00:00

abstract：:Recognition for proteolysis by trypsin depends almost exclusively on tight binding of arginine or lysine side chains by the primary substrate specificity pocket. Although extended subsite interactions are important for catalysis, the majority of binding energy is localized in the P1 pocket. Analysis of the interaction...

journal_title：Biochemistry

authors： Willett WS,Brinen LS,Fletterick RJ,Craik CS

更新日期：1996-05-14 00:00:00

abstract：:Cytomegaloviruses (CMVs) are ubiquitous species-specific viruses that establish acute, persistent, and latent infections. Both human and mouse CMVs encode proteins that inhibit the activation of natural killer (NK) cells by downregulating cellular ligands for the NK cell activating receptor, NKG2D. The MCMV glycoprote...

journal_title：Biochemistry

authors： Zhi L,Mans J,Paskow MJ,Brown PH,Schuck P,Jonjić S,Natarajan K,Margulies DH

更新日期：2010-03-23 00:00:00

abstract：:Dehydroquinate synthase has been purified 9000-fold from Escherichia coli K-12 (strain MM294). The synthase is encoded by the aroB gene, which is carried by plasmid pLC29-47 from the Carbon-Clarke library. Construction of an appropriate host bearing pLC29-47 results in a strain that produces 20 times more enzyme than ...

journal_title：Biochemistry

authors： Frost JW,Bender JL,Kadonaga JT,Knowles JR

更新日期：1984-09-11 00:00:00

abstract：:Michler's hydrol blue (MHB) is investigated with respect to photophysical properties in varied solvent environment and when bound to insulin and lysozyme fibrils. The MHB chromophore is shown to act like a molecular rotor and bind well to amyloid fibrils, where it exhibits a characteristic red-shift in its excitation ...

journal_title：Biochemistry

authors： Kitts CC,Beke-Somfai T,Nordén B

更新日期：2011-05-03 00:00:00

abstract：:A mutant form of the nitrogenase iron protein with a deletion of residue Leu 127, located in the switch II region of the nucleotide binding site, forms a tight, inactive complex with the nitrogenase molybdenum iron (MoFe) protein in the absence of nucleotide. The structure of this complex generated with proteins from ...

journal_title：Biochemistry

authors： Chiu H,Peters JW,Lanzilotta WN,Ryle MJ,Seefeldt LC,Howard JB,Rees DC

更新日期：2001-01-23 00:00:00

abstract：:Isolation and characterization of a novel radiation-induced product, i.e., the 8-hydroxyguanine residue, produced in deoxyribonucleic acid (DNA), 2'-deoxyguanosine, and 2'-deoxyguanosine 5'-monophosphate by gamma-irradiation in aqueous solution, are described. For this purpose, gamma-irradiated DNA was first hydrolyze...

journal_title：Biochemistry

authors： Dizdaroglu M

更新日期：1985-07-30 00:00:00

abstract：:The ubiquitous calcium-binding protein calmodulin (CaM) regulates a wide variety of cellular events by binding to and activating many distinct target enzymes. The CaM-binding domains of most of these enzymes are contained in a contiguous stretch of amino acids with a length of approximately 20 residues. In this work, ...

journal_title：Biochemistry

authors： Zhang M,Fabian H,Mantsch HH,Vogel HJ

更新日期：1994-09-13 00:00:00

abstract：:Various agents are able to stimulate the EGF receptor protein tyrosine kinase in the absence of ligand binding. To characterize their mechanism of action, we investigated their effects on the kinase activity of the intracellular domain of the EGF receptor (EGFR-IC). EGFR-IC (67 kDa) lacking the extracellular domain an...

journal_title：Biochemistry

authors： Mohammadi M,Honegger A,Sorokin A,Ullrich A,Schlessinger J,Hurwitz DR

更新日期：1993-08-31 00:00:00

abstract：:Despite many studies suggesting the role of enolpyruvate as a bound intermediate in the pyruvate kinase reaction, direct evidence for it has been lacking. By use of a combination of chemical trapping and isolation of a derivative, significant amounts of enzyme-bound enolpyruvate have now been demonstrated. The method ...

journal_title：Biochemistry

authors： Seeholzer SH,Jaworowski A,Rose IA

更新日期：1991-01-22 00:00:00

abstract：:Legumain (asparaginyl endopeptidase) is the only protease with a preference for cleavage after asparagine residues. Increased legumain activity is a hallmark of inflammation, neurodegenerative diseases, and cancer, and legumain inhibitors have exhibited therapeutic effects in mouse models of these pathologies. Improve...

journal_title：Biochemistry

authors： Anderson BM,de Almeida LGN,Sekhon H,Young D,Dufour A,Edgington-Mitchell LE

更新日期：2020-01-28 00:00:00

abstract：:The structure of octylcarbamoyl-alpha-chymotrypsin to a resolution of 3.0 A is described. The n-octyl side chain of the active site directed irreversible inactivator octyl isocyanate is bound exclusively in the hydrophobic substrate binding pocket. The n-octyl isocyanate forms a planar urethane bond with the Ser-195 O...

journal_title：Biochemistry

authors： Brown WE

更新日期：1975-11-18 00:00:00

abstract：:Twelve site-directed mutants of rat cytochrome P450 2B1 distributed over seven positions and four putative substrate recognition sites (SRS) were constructed and expressed in COS cells. Function was examined using androstenedione and testosterone as substrates. Substitutions at positions 303, 360, and 473 did not mark...

journal_title：Biochemistry

authors： He Y,Luo Z,Klekotka PA,Burnett VL,Halpert JR

更新日期：1994-04-12 00:00:00

abstract：:We have used time-resolved phosphorescence anisotropy to investigate the effects of essential light chain (ELC) isoforms (A1 and A2) on the interaction of skeletal muscle myosin with actin, to relate structural dynamics to previously reported functional effects. Actin was labeled with a phosphorescent probe at C374, a...

journal_title：Biochemistry

authors： Prochniewicz E,Guhathakurta P,Thomas DD

更新日期：2013-03-05 00:00:00

abstract：:Phase-sensitive 2D 1H/1H COSY spectra can be used to identify the structures of individual pure specimens of the aminoglycoside antibiotic amikacin and its N-hemisuccinyl derivatives. However, even at 500 MHz the 2D chemical shift dispersion does not allow for unambiguous assignment of all cross-peaks. By use of 2D re...

journal_title：Biochemistry

authors： Andersen NH,Eaton HL,Nguyen KT,Hartzell C,Nelson RJ,Priest JH

更新日期：1988-04-19 00:00:00

abstract：:Although the Escherichia coli fatty acid synthesis (FAS) pathway is the best studied type II fatty acid synthesis system, a major experimental limitation has been the inability to feed intermediates into the pathway in vivo because exogenously supplied free fatty acids are not efficiently converted to the acyl-acyl ca...

journal_title：Biochemistry

authors： Jiang Y,Morgan-Kiss RM,Campbell JW,Chan CH,Cronan JE

更新日期：2010-02-02 00:00:00

abstract：:Tripeptidyl peptidase II is a high molecular weight serine exopeptidase, which has been purified from rat liver and human erythrocytes. Four clones, representing 4453 bp, or 90% of the mRNA of the human enzyme, have been isolated from two different cDNA libraries. One clone, designated A2, was obtained after screening...

journal_title：Biochemistry

authors： Tomkinson B,Jonsson AK

更新日期：1991-01-08 00:00:00

abstract：:Class II MHC glycoproteins bind short (7-25 amino acid) peptides in an extended type II polyproline-like conformation and present them for immune recognition. Because empty MHC is unstable, measurement of the rate of the second-order reaction between peptide and MHC is challenging. In this report, we use dissociation ...

journal_title：Biochemistry

authors： Kasson PM,Rabinowitz JD,Schmitt L,Davis MM,McConnell HM

更新日期：2000-02-08 00:00:00

abstract：:Antithrombin III (ATIII) is a plasma-borne serine protease inhibitor that apparently forms covalent complexes with thrombin. The interaction between ATIII and thrombin is enhanced several thousandfold by the glycosaminoglycan, heparin. We have previously proposed that the heparin binding site of ATIII resides within a...

journal_title：Biochemistry

authors： Smith JW,Dey N,Knauer DJ

更新日期：1990-09-25 00:00:00

abstract：:Site-directed mutagenesis has been used to replace alanine 305 with phenylalanine (A305F) and serine (A305S) in the active site of cytochrome P450 3A4 (CYP3A4). Enzyme kinetics for diazepam, erythromycin, nifedipine, and testosterone metabolism have been determined for both mutants and wild-type CYP3A4. The A305F muta...

journal_title：Biochemistry

authors： Fowler SM,Riley RJ,Pritchard MP,Sutcliffe MJ,Friedberg T,Wolf CR

更新日期：2000-04-18 00:00:00

abstract：:Excluded volume and viscosity effects of crowding agents that mimic crowded conditions in vivo on "classical" burst phase folding kinetics of cytochrome c are assessed in vitro. Upon electron transfer-triggered folding of reduced cytochrome c, far-UV time-resolved circular dichroism (TRCD) is used to monitor folding u...

journal_title：Biochemistry

authors： Chen E,Christiansen A,Wang Q,Cheung MS,Kliger DS,Wittung-Stafshede P

更新日期：2012-12-11 00:00:00

abstract：:Kinetic and structural changes in recombinant human aldose reductase (AR) due to modification by S-nitrosoglutathione (GSNO) were investigated. Incubation of the enzyme with 10-50 microM GSNO led to a time- and concentration-dependent inactivation of the enzyme, with a second-order rate constant of 0.087 +/- 0.009 M-1...

journal_title：Biochemistry

authors： Chandra A,Srivastava S,Petrash JM,Bhatnagar A,Srivastava SK

更新日期：1997-12-16 00:00:00

abstract：:The protein tyrosine kinase, pp60(c-)(src), is involved in cellular signaling and is activated during mitosis and in various tumors. We have been employing cyclic decapeptides to identify the determinants for substrate binding and phosphorylation to develop inhibitors competitive with protein substrates of Src. A stru...

journal_title：Biochemistry

authors： Wang W,Ramdas L,Sun G,Ke S,Obeyesekere NU,Budde RJ,McMurray JS

更新日期：2000-05-02 00:00:00

abstract：:Time-resolved decays of fluorescence anisotropy were obtained from frequency-domain measurements of the phase angle difference between the parallel and perpendicular components of the polarized emission and the ratio of the modulated amplitudes. These data were measured at modulation frequencies ranging from 1 to 200 ...

journal_title：Biochemistry

authors： Lakowicz JR,Cherek H,Maliwal BP

更新日期：1985-01-15 00:00:00

abstract：:The modified Tanford-Kirkwood electrostatic theory (Shire et al., 1974a) was applied to ferrimyoglobins from the following animal species: sperm whale (Physeter catodon), horse, California grey whale (Eschrichtius gibbosus), harbor seal (Phoca vitulina), and California sea lion (Zalophus californianus). Computations w...

journal_title：Biochemistry

authors： Shire SJ,Hanania GI,Gurd FR

更新日期：1975-04-08 00:00:00

abstract：:Cardiac troponin T (cTnT) is a myofibrillar protein essential for calcium-dependent contraction. This property has led to functional studies of developmentally expressed cTnT isoforms and mutants identified in patients with hypertrophic cardiomyopathy. The release of cTnT into the serum following myocardial infarction...

journal_title：Biochemistry

authors： Lounes KC,Demeler B,Anderson DE,Gomes AW,Potter JD,Nassar R,Anderson PA

更新日期：2008-02-19 00:00:00

abstract：:In contrast to FK506 binding proteins and cyclophilins, the parvulin family of peptidyl-prolyl cis/trans isomerases (PPIases; E.C. 5.2.1.8) cannot be inhibited by either FK506 or cyclosporin A. We have found that juglone, 5-hydroxy-1,4-naphthoquinone, irreversibly inhibits the enzymatic activity of several parvulins, ...

journal_title：Biochemistry

authors： Hennig L,Christner C,Kipping M,Schelbert B,Rücknagel KP,Grabley S,Küllertz G,Fischer G

更新日期：1998-04-28 00:00:00

abstract：:We have used enzymic and chemical probes to search for altered DNA conformations in the 5' flanking region of the gene for a high mobility group protein (HMG-T) from trout. This search was conducted in order to identify potential genetic elements that might be involved in the transcriptional control of the HMG-T gene....

journal_title：Biochemistry

authors： Wright JM,Dixon GH

更新日期：1988-01-26 00:00:00

abstract：:The structure of human epidermal growth factor (EGF, 53 amino acids) comprises three distinct loops (A, B, and C) connected correspondingly by the three native disulfide bonds, Cys(6)-Cys(20), Cys(14)-Cys(31), and Cys(33)-Cys(42). The connection of Cys(6) and Cys(20) forming the N-terminal A loop is essential for the ...

journal_title：Biochemistry

authors： Lu BY,Jiang C,Chang JY

更新日期：2005-11-15 00:00:00