Alkyl isocyanates as active site-specific reagents for serine proteases. Location of alkyl binding site in chymotrypsin by X-ray diffraction.

abstract：:The active site of histidine decarboxylase (HDC) from Lactobacillus 30a contains a pyruvoyl cofactor sitting at the interface of two molecules in a trimer. Although exhibiting hyperbolic kinetics at pH 4.8, near its optimum, HDC is cooperative at pH 7.6, indicating that the units of the trimer communicate. A Hill plot...

journal_title：Biochemistry

authors： Pishko EJ,Robertus JD

更新日期：1993-05-11 00:00:00

abstract：:Human type beta 2 transforming growth factor (hTGF-beta 2) was purified from tamoxifen-supplemented, serum-free medium conditioned by the human prostatic adenocarcinoma cell line PC-3. The purification of hTGF-beta 2 was monitored in a growth inhibition assay and was achieved by batch purification on methylsilyl-contr...

journal_title：Biochemistry

authors： Ikeda T,Lioubin MN,Marquardt H

更新日期：1987-05-05 00:00:00

abstract：:Poly(ethylene glycol) (PEG)-mediated fusion of phosphatidylcholine model membranes has been shown to mimic the protein-mediated biomembrane process [Lee, J., and Lentz, B. R. (1998) Proc. Natl. Acad. Sci. U.S.A. 95, 9274-9279]. Unlike the simple model membranes used in this earlier study, the lipid composition of fuso...

journal_title：Biochemistry

authors： Haque ME,McIntosh TJ,Lentz BR

更新日期：2001-04-10 00:00:00

abstract：:Most RNA molecules that are endowed with catalytic activity function in the form of ribonucleoproteins within cells. These complexes are frequently large, poorly defined, and difficult to study. As a model system to study biological catalysis by ribonucleoproteins, we have modified the hairpin ribozyme by inserting an...

journal_title：Biochemistry

authors： Sargueil B,Pecchia DB,Burke JM

更新日期：1995-06-13 00:00:00

abstract：:The stimulatory GTP-binding protein of adenylate cyclase, Gs, and beta-adrenergic receptors were reconstituted into unilamellar phospholipid vesicles. The kinetics of the quasiirreversible binding of guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S) to Gs, equivalent to Gs activation by nucleotide, was studied with re...

journal_title：Biochemistry

authors： Asano T,Ross EM

更新日期：1984-11-06 00:00:00

abstract：:The objective of this work is to determine the molecular mechanism and regulation of short-chain acyl-CoA dehydrogenase (SCAD) from Megasphaera elsdenii. To achieve this, the gene coding for SCAD from M. elsdenii was cloned and sequenced. Site-directed mutagenesis was then used to identify an amino acid residue that i...

journal_title：Biochemistry

authors： Becker DF,Fuchs JA,Banfield DK,Funk WD,MacGillivray RT,Stankovich MT

更新日期：1993-10-12 00:00:00

abstract：:Although G protein-coupled receptor (GPCR) kinases (GRKs) have been shown to mediate desensitization of numerous GPCRs in studies using cellular expression systems, their function under physiological conditions is less well understood. In the current study, we employed various strategies to assess the effect of inhibi...

journal_title：Biochemistry

authors： Kong KC,Gandhi U,Martin TJ,Anz CB,Yan H,Misior AM,Pascual RM,Deshpande DA,Penn RB

更新日期：2008-09-02 00:00:00

abstract：:In this work, four single tryptophan (Trp) mutants of the dimeric mannitol transporter of Escherichia coli, EII(mtl), are characterized using Trp and 5-fluoroTrp (5-FTrp) fluorescence spectroscopy. The four positions, 97, 114, 126, and 133, are located in a region shown by recent studies to be involved in the mannitol...

journal_title：Biochemistry

authors： Vos EP,Bokhove M,Hesp BH,Broos J

更新日期：2009-06-16 00:00:00

abstract：:We have investigated the effects of mixed Na+: Mg2+ ionic solutions on the stability of the nonspecific lac repressor-DNA complex. The effects of Mg2+ are simply interpreted in terms of its role as a competitor (with repressor) for DNA sites. From these studies, the binding constant of the Mg-DNA complex can be determ...

journal_title：Biochemistry

authors： Record MT Jr,deHaseth PL,Lohman TM

更新日期：1977-11-01 00:00:00

abstract：:In a family of proteins, often the three-dimensional structure has been experimentally determined only for one member or a few members of the family. Homology modeling can be used to model the structures of all other members of the family and thus allow comparison of these structures. This approach was applied to hete...

journal_title：Biochemistry

authors： Kosloff M,Elia N,Selinger Z

更新日期：2002-12-10 00:00:00

abstract：:Z-disc-associated, alternatively spliced, PDZ motif-containing protein (ZASP) is a principal component of the sarcomere. The three prevalent isoforms of ZASP in skeletal muscle are generated by alternative splicing of exons 9 and 10. The long isoforms, either having (ZASP-L) or lacking exon 10 (ZASP-LΔex10), include a...

journal_title：Biochemistry

authors： Watts NR,Zhuang X,Kaufman JD,Palmer IW,Dearborn AD,Coscia S,Blech-Hermoni Y,Alfano C,Pastore A,Mankodi A,Wingfield PT

更新日期：2017-04-11 00:00:00

abstract：:An NMR study has been made of porcine pancreatic phospholipase A2 (PLA) in three environments: free in solution, in a binary complex with dodecylphosphocholine micelles, and in a ternary complex with a micelle and the substrate-like inhibitor (R)-1-octyl-2-(N-dodecanoylamino)-2-deoxyglycero-3-phosph oglycol. 1H and 15...

journal_title：Biochemistry

authors： Peters AR,Dekker N,van den Berg L,Boelens R,Kaptein R,Slotboom AJ,de Haas GH

更新日期：1992-10-20 00:00:00

abstract：:The effects of ionophore A23187 on the movements of Ca2+, Mg2+, H+ phosphate, and succinate and its effects on energy utilization by mitochondria have been studied as a function of ionophore concentrations. At a low ratio of the compound to mitochondrial protein, below that required for maximal uncoupling, an apparent...

journal_title：Biochemistry

authors： Pfeiffer DR,Hutson SM,Kauffman RF,Lardy HA

更新日期：1976-06-15 00:00:00

abstract：:The affinity of various carbohydrates for the galactose/N-acetylgalactosamine-specific lectin of the rabbit liver was assessed by determining the effect of these carbohydrates on the binding of [125I]asialoorosomucoid (125I-ASOR) by the lectin. To obtain the concentration of the inhibitor that causes 50% reduction in ...

journal_title：Biochemistry

authors： Lee RT,Myers RW,Lee YC

更新日期：1982-11-23 00:00:00

abstract：:The solution structure of the C-terminal fragment 255-316 of thermolysin has been determined by two-dimensional proton NMR methods. For this disulfide-free fragment there was a previous proposal according to which it would fold into a stable helical structure forming a dimer at concentrations above 0.06 mM. A complete...

journal_title：Biochemistry

authors： Rico M,Jiménez MA,González C,De Filippis V,Fontana A

更新日期：1994-12-13 00:00:00

abstract：:Endonexin (annexin IV) is a member of the annexin family of homologous proteins that bind membranes and aggregate vesicles in a calcium-dependent fashion. This study examines the lipid modulation and mechanism of the binding of endonexin to membranes using a fluorescence energy transfer assay to measure bovine endonex...

journal_title：Biochemistry

authors： Junker M,Creutz CE

更新日期：1994-08-02 00:00:00

abstract：:The beta chain 15-42 sequence of the fibrin(ogen) E region was implicated in heparin binding [Odrljin et al. (1996) Blood 88, 2050-2061]; whether heparin binds to other fibrin(ogen) regions remains to be clarified. To address this question, we studied the interaction of heparin with fibrinogen, fibrin, and their major...

journal_title：Biochemistry

authors： Yakovlev S,Gorlatov S,Ingham K,Medved L

更新日期：2003-07-01 00:00:00

abstract：:An overexpression system for spinach apocytochrome b(6) as a fusion protein to a maltose-binding protein in Escherichia coli was established using the expression vector pMalp2. The fusion of the cytochrome b(6) to the periplasmic maltose-binding protein directs the cytochrome on the Sec-dependent pathway. The cytochro...

journal_title：Biochemistry

authors： Króliczewski J,Hombek-Urban K,Szczepaniak A

更新日期：2005-05-24 00:00:00

abstract：:The effect of myosin on the structure of two sequences on G-actin, a loop between residues 39 and 52 and a segment between residues 61 and 69 from the NH2-terminus, was probed by limited proteolytic digestions of G-actin in the presence of the myosin subfragment 1 isozyme S-1(A2). Under the experimental conditions of ...

journal_title：Biochemistry

authors： Chen T,Haigentz M Jr,Reisler E

更新日期：1992-03-24 00:00:00

abstract：:R67 dihydrofolate reductase (DHFR) is a type II DHFR produced by bacteria as a resistance mechanism to the increased clinical use of the antibacterial drug trimethoprim. Type II DHFRs are not homologous in either sequence or structure with chromosomal DHFRs. The type II enzymes contain four identical subunits which fo...

journal_title：Biochemistry

authors： Li D,Levy LA,Gabel SA,Lebetkin MS,DeRose EF,Wall MJ,Howell EE,London RE

更新日期：2001-04-10 00:00:00

abstract：:Depletion of Ca2+ and/or Cl- ions from PSII membranes blocks the electron-transfer reactions that precede O2 evolution on the oxidizing side of the enzyme. Illumination of these inhibited preparations at 273 K generates a paramagnetic species that is detectable by low-temperature (T < 20 K) EPR as a signal in the g = ...

journal_title：Biochemistry

authors： Lydakis-Simantiris N,Dorlet P,Ghanotakis DF,Babcock GT

更新日期：1998-05-05 00:00:00

abstract：:The direct electrochemistry of the cytochrome c/cytochrome b5 and cytochrome c/plastocyanin complexes has been investigated at edge-plane graphite and modified gold electrode surfaces, which are selective for one of the two components of the complex. Electrochemical response of one protein at an otherwise electrostati...

journal_title：Biochemistry

authors： Bagby S,Barker PD,Guo LH,Hill HA

更新日期：1990-04-03 00:00:00

abstract：:A recombinant form of Rhodobacter sphaeroides phosphoribulokinase (form I; NADH dependent) has been expressed in and purified to homogeneity from Escherichia coli that harbor the prkA gene in the plasmid pKP1565b. Restriction digestion of the phosphoribulokinase-encoding plasmid produces a tractable 450 bp fragment th...

journal_title：Biochemistry

authors： Sandbaken MG,Runquist JA,Barbieri JT,Miziorko HM

更新日期：1992-04-14 00:00:00

abstract：:Toxic shock syndrome toxin-1 (TSST-1), the potent staphylococcal exoprotein linked to most cases of the toxic shock syndrome, is a V beta-restricted T-cell mitogen (a so-called "superantigen"). TSST-ovine (TSST-O) is a natural variant of TSST-1, and is produced by certain ovine mastitis-associated strains of Staphyloc...

journal_title：Biochemistry

authors： Deresiewicz RL,Woo J,Chan M,Finberg RW,Kasper DL

更新日期：1994-11-01 00:00:00

abstract：:The structure of an analogue of the yeast tRNAPhe T Psi C stem-loop has been determined by NMR spectroscopy and restrained molecular dynamics. The molecule contained the highly conserved modification ribothymidine at its naturally occurring position. The ribothymidine-modified T Psi C stem-loop is the product of the m...

journal_title：Biochemistry

authors： Koshlap KM,Guenther R,Sochacka E,Malkiewicz A,Agris PF

更新日期：1999-07-06 00:00:00

abstract：:Fusion of the human immunodeficiency virus (HIV) with target cells is mediated by the gp41 subunit of the envelope protein. Mutation and deletion studies within the transmembrane domain (TMD) of intact gp41 influenced its fusion activity. In addition, current models suggest that the TMD is in proximity with the fusion...

journal_title：Biochemistry

authors： Reuven EM,Dadon Y,Viard M,Manukovsky N,Blumenthal R,Shai Y

更新日期：2012-04-03 00:00:00

abstract：:RNA binding proteins play an important role in regulating alternative pre-mRNA splicing and in turn cellular gene expression. Many of these RNA binding proteins occur as gene families with members sharing a high degree of primary structure identity and domain organization yet have tissue-specific expression patterns a...

journal_title：Biochemistry

authors： Pina JM,Reynaga JM,Truong AAM,Keppetipola NM

更新日期：2018-07-03 00:00:00

abstract：:The multifunctional proline utilization A (PutA) flavoenzyme from Escherichia coli catalyzes the oxidation of proline to glutamate in two reaction steps using separate proline dehydrogenase (PRODH) and Δ(1)-pyrroline-5-carboxylate (P5C) dehydrogenase domains. Here, the kinetic mechanism of PRODH in PutA is studied by ...

journal_title：Biochemistry

authors： Moxley MA,Becker DF

更新日期：2012-01-10 00:00:00

abstract：:Cyclic AMP receptor protein is involved in the regulation of more than 20 genes. A step in the mechanism of activation of transcription is to induce a significant bending of the DNA upon complex formation between specific DNA and the protein. The induced DNA bending and a structure of the protein-DNA complex were stud...

journal_title：Biochemistry

authors： Heyduk T,Lee JC

更新日期：1992-06-09 00:00:00

abstract：:The binding of 8-anilino-1-naphthalenesulfonate (ANS) to ciliary dynein ATPase leads to a marked increase in the dye's fluorescence intensity, accompanied by a blue shift in the observed fluorescence emission maximum. We found that dynein has 37 +/- 3 ANS binding sites and that experimentally applied ANS concentration...

journal_title：Biochemistry

authors： Saucier AC,Mariotti S,Anderson SA,Purich DL

更新日期：1985-12-17 00:00:00