Abstract:
:Fusion of the human immunodeficiency virus (HIV) with target cells is mediated by the gp41 subunit of the envelope protein. Mutation and deletion studies within the transmembrane domain (TMD) of intact gp41 influenced its fusion activity. In addition, current models suggest that the TMD is in proximity with the fusion peptide (FP) at the late fusion stages, but there are no direct experimental data to support this hypothesis. Here, we investigated the TMD focusing on two regions: the N-terminal containing the GxxxG motif and the C-terminal containing the GLRI motif, which is conserved among the TMDs of HIV and the T-cell receptor. Studies utilizing the ToxR expression system combined with synthetic peptides and their fluorescent analogues derived from TMD revealed that the GxxxG motif is important for TMD self-association, whereas the C-terminal region is for its heteroassociation with FP. Functionally, all three TMD peptides induced lipid mixing that was enhanced significantly upon mixing with FP. Furthermore, the TMD peptides inhibited virus-cell fusion apparently through their interaction with their endogenous counterparts. Notably, the R2E mutant (in the GLRI) was significantly less potent than the two others. Overall, our findings provide experimental evidence that HIV-1 TMD contributes to membrane assembly and function of the HIV-1 envelope. Owing to similarities between functional domains within viruses, these findings suggest that the TMDs and FPs may contribute similarly in other viruses as well.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Reuven EM,Dadon Y,Viard M,Manukovsky N,Blumenthal R,Shai Ydoi
10.1021/bi201721rsubject
Has Abstractpub_date
2012-04-03 00:00:00pages
2867-78issue
13eissn
0006-2960issn
1520-4995journal_volume
51pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Serine hydroxymethyltransferase and C1-tetrahydrofolate synthase catalyze four reactions which convert formate and glycine to serine. The one-carbon carrier in these reactions if tetrahydropteroylglutamate which is regenerated in the coupled reaction and thus can be used in catalytic concentrations with respect to ser...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00450a028
更新日期:1989-11-28 00:00:00
abstract::Two major neutral glyceroglucolipids (A and B) have been isolated from lipid extract of human gastric content by the procedure involving column fractionation on DEAE-Sephadex, silicic acid, and thin-layer chromatography. Both glycolipids contained glucose, glyceryl ethers, and fatty acids. The structures of these glyc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00637a003
更新日期:1977-09-06 00:00:00
abstract::The role of the protein shell in the formation of the hydrous ferric oxide core of ferritin is poorly understood. A VO2+ spin probe study was undertaken to characterize the initial complex of Fe2+ with horse spleen apoferritin (96% L-subunits). A competitive binding study of VO2+ and Fe2+ showed that the two metals co...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00102a012
更新日期:1991-09-24 00:00:00
abstract::S100A6 is a member of the S100 subfamily of EF-hand Ca (2+) binding proteins that has been shown to interact with calcyclin binding protein/Siah-1 interacting protein (CacyBP/SIP or SIP), a subunit of an SCF-like E3 ubiquitin ligase complex (SCF-TBL1) formed under genotoxic stress. SIP serves as a scaffold in this com...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi801233z
更新日期:2008-10-14 00:00:00
abstract::Kinetics of flash-induced electron transfer from the soluble cytochrome c2 to the primary donor (P) of the reaction center purified from the purple bacterium Rhodobacter sphaeroides R-26 were investigated by time-resolved absorption spectroscopy. Re-reduction of P+ induced by a laser pulse was measured at 1283 nm both...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00211a037
更新日期:1993-12-07 00:00:00
abstract::DNA polymerase delta from calf thymus has been purified to apparent homogeneity by a new procedure which utilizes hydrophobic interaction chromatography with phenyl-Sepharose at an early step to separate most of the calcium-dependent protease activity from DNA polymerase delta and alpha. The purified enzyme migrates a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00304a003
更新日期:1984-04-24 00:00:00
abstract::The crystal structures of the guanosine 5'-monophosphate (GMP) and inosine 5'-monophosphate (IMP) complexes of Toxoplasma gondii hypoxanthine-guanine phosphoribosyltransferase (HGPRT) have been determined at 1.65 and 1.90 A resolution. These complexes, which crystallize in space groups P2(1) (a = 65.45 A, b = 90.84 A,...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990507q
更新日期:1999-11-02 00:00:00
abstract::Using cysteine mutagenesis and chemical modification by methanethiosulfonate derivatives, it was demonstrated that the external putative loop, joining transmembrane segments (TM's) IV-V of rabbit Na+/glucose cotransporter, rSGLT1, forms part of a Na+ binding and voltage sensing domain. Within this region, exposure to ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi052267m
更新日期:2006-04-11 00:00:00
abstract::Type 1 diabetes (T1D) is an autoimmune disease characterized by an insulin deficiency. Ever since the discovery of insulin almost 100 years ago, patients with T1D have relied on multiple daily insulin injections to survive an otherwise deadly disease. Despite decades of research and clinical trials, no treatment exist...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/acs.biochem.9b00707
更新日期:2019-10-08 00:00:00
abstract::DNA polymerases slide on DNA during replication, and the interface must be mobile for various conformational changes. The role of lubricant interfacial water is not understood. In this report, we systematically characterize the water dynamics at the interface and in the active site of a tight binding polymerase (pol β...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500810a
更新日期:2014-08-26 00:00:00
abstract::We report a new observation of the role of Escherichia coli single-strand DNA binding protein (SSB) in synthesis of primer RNA (pRNA) catalyzed by.E.coli primase on the SSB-coated phage G4oric template. Using a set of ATP priming substrates with reactive groups attached to the 5' gamma-phosphate on different length "a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972455f
更新日期:1998-03-17 00:00:00
abstract::Previous studies of the low molecular mass family 11 xylanase from Bacillus circulans show that the ionization state of the nucleophile (Glu78, pK(a) 4.6) and the acid/base catalyst (Glu172, pK(a) 6.7) gives rise to its pH-dependent activity profile. Inspection of the crystal structure of BCX reveals that Glu78 and Gl...
journal_title:Biochemistry
pub_type: 杂志文章
doi:
更新日期:2001-08-28 00:00:00
abstract::We have studied the role of amphipathic alpha-helices in the ability of apoA-I to promote cholesterol efflux from human skin fibroblasts and activate lecithin:cholesterol acyltransferase (LCAT). Three apoA-I mutants were designed, each by deletion of a pair of predicted adjacent central alpha-helices [Delta(100-143), ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981205b
更新日期:1998-09-29 00:00:00
abstract::Type IIS restriction endonuclease Eco31I harbors a single HNH active site and cleaves both DNA strands close to its recognition sequence, 5'-GGTCTC(1/5). A two-domain organization of Eco31I was determined by limited proteolysis. Analysis of proteolytic fragments revealed that the N-terminal domain of Eco31I is respons...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi800660u
更新日期:2008-08-19 00:00:00
abstract::The integral membrane protein diacylglycerol kinase (DGK) from Escherichia coli has been reversibly unfolded in a protein/detergent/mixed micelle system by varying the molar ratio of n-decyl beta-D-maltoside (DM) and sodium dodecyl sulfate (SDS). Unfolding was monitored by circular dichroism (CD) and ultraviolet (UV) ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi963095j
更新日期:1997-05-13 00:00:00
abstract::The high-frequency resonance Raman spectra of FeIII yeast native cytochrome c peroxidase (CCP) and five of its mutants [CCP(MI), Phe-51, Leu-48, Lys-48, Asn-235, and Phe-191] were recorded in phosphate buffer, pH 7.0, and in glycerol/phosphate mixtures at 295 and 10 K. Glycerol induces heme coordination changes in som...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00438a024
更新日期:1989-06-13 00:00:00
abstract::Electron spin polarized electron paramagentic resonance (ESP EPR) spectra were obtained with deuterated iron-removed photosynthetic bacterial reaction centers (RCs) to specifically investigate the effect of the rate of primary charge separation, metal-site occupancy, and H-subunit content on the observed P865+QA- char...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9630319
更新日期:1997-07-15 00:00:00
abstract::High-resolution phosphorus-31 nuclear magnetic resonance (31P NMR) spectra of wild-type and mutant strains of Saccharomyces cerevisiae were observed at a frequency of 145.7 MHz. Levels of various phosphorus metabolites were investigated upon addition of glucose under both aerobic and anaerobic conditions. Three mutant...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00588a006
更新日期:1979-10-16 00:00:00
abstract::An in vitro system reconstituted with mouse liver polysome translation products was used to study the nature of polypeptide species imported into mitochondria from different mouse tissues such as liver, kidney, brain, and heart, as well as from Ehrlich ascites, Novikoff hepatoma, and Morris hepatoma 3924A tumor lines....
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00348a041
更新日期:1985-12-31 00:00:00
abstract::The cyclic decapeptide antibiotic tyrocidine A was studied by two relatively new methods, viz., correlation proton magnetic resonance (pmr) spectroscopy and double-resonance difference pmr spectroscopy. The correlation method of spectral accumulation provided pmr spectra of good resolution, and in addition the signal-...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00673a032
更新日期:1975-01-28 00:00:00
abstract::We have investigated the kinetic and thermodynamic properties of carbon monoxide binding to the fully reduced quinol oxidase (cytochrome aa(3)) from the hyperthermophilic archaeon Acidianus ambivalens. After flash photolysis of CO from heme a(3), the complex recombines with an apparent rate constant of approximately 3...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990473m
更新日期:1999-08-03 00:00:00
abstract::The fluorescence self-quenching by energy transfer of FITC-C9, a fluoresceinated derivative of human complement protein C9 [Sims, P.J. (1984) Biochemistry (preceding paper in this issue)], has been used to monitor the kinetics of C9 polymerization induced by the membrane-associated complex of complement proteins C5b-8...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00309a021
更新日期:1984-07-03 00:00:00
abstract::Retinal rod and cone cGMP phosphodiesterases (PDE6 family) function as the effector enzyme in the vertebrate visual transduction cascade. The activity of PDE6 catalytic subunits is controlled by the Pgamma-subunits. In addition to the inhibition of cGMP hydrolysis at the catalytic sites, Pgamma is known to stimulate a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi015935m
更新日期:2002-03-26 00:00:00
abstract::With respect to the functional importance attributed to the N-terminal part of the Na(+)/proline transporter of Escherichia coli (PutP), we report here on the structural arrangement and functional dynamics of transmembrane domains (TMs) II and III and the adjoining loop regions. Information on membrane topography was ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi992442x
更新日期:2000-04-25 00:00:00
abstract::The local environment of the transmembrane and C-terminal domain of M13 major coat protein was probed by site-directed ESR spin labeling when the protein was introduced into three membrane-mimicking systems, DOPC vesicles, sodium cholate micelles, and SDS micelles. For this purpose, we have inserted unique cysteine re...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961770j
更新日期:1996-12-03 00:00:00
abstract::By classical competitive antagonism, a substrate and competitive inhibitor must bind mutually exclusively to the active site. The competitive inhibition of O-acetyl serine sulfhydrylase (OASS) by the C-terminus of serine acetyltransferase (SAT) presents a paradox, because the C-terminus of SAT binds to the active site...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00500
更新日期:2017-09-19 00:00:00
abstract::Myelin basic protein (MBP) is a multifunctional protein involved in maintaining the stability and integrity of the myelin sheath by a variety of interactions with membranes and with cytoskeletal and other proteins. A central segment of MBP is highly conserved in mammals and consists of a membrane surface-associated am...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi701336n
更新日期:2008-01-08 00:00:00
abstract::Recent studies have shown that there can be large differences in the stability of double and triple helical nucleic acid complexes, depending on whether RNA or DNA strands are involved. These differences have been attributed to structural differences in the sugar-phosphate backbone of these two polymers. However, sinc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00012a031
更新日期:1995-03-28 00:00:00
abstract::Diverse voltage-gated K+ channels are produced by tetramerization of channel alpha subunits that can be encoded by multiple genes. In GH3 pituitary cells, the Kv1.4 and Kv1.5 subunit genes are constitutively expressed, and dexamethasone selectively up-regulates expression of the latter gene. Here we use subunit-specif...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961290s
更新日期:1996-11-12 00:00:00
abstract::Cytochrome c6 from Monoraphidium braunii, an 89-amino acid electron transfer protein, has been investigated by NMR in solution, in its oxidized form, at pH 7 and 300 K. By using a combination of COSY, TOCSY, and NOESY experiments, 84% of the proton resonances have been assigned. A total of 1668 experimental NOE constr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972765y
更新日期:1998-04-07 00:00:00