Abstract:
:An in vitro system reconstituted with mouse liver polysome translation products was used to study the nature of polypeptide species imported into mitochondria from different mouse tissues such as liver, kidney, brain, and heart, as well as from Ehrlich ascites, Novikoff hepatoma, and Morris hepatoma 3924A tumor lines. Mouse hepatic mitochondria import a number of proteins including 160-kilodalton (kDa) carbamoyl-phosphate synthetase I (CPS-I). Two other proteins of 63 and 57 kDa of unknown function are also imported as major components by mouse liver mitochondria. Under these in vitro conditions, however, mitochondria from non-CPS-I expressing tissues such as brain, kidney, and heart failed to import and process the precursor forms of CPS-I (pCPS-I). Furthermore, mitochondria from three different tumor lines (Novikoff hepatoma, Morris hepatoma, and Ehrlich ascites) containing negligible CPS-I activity were also unable to import and process pCPS-I to any significant level. Similarly, the 63-kDa protein was selectively transported into liver and kidney mitochondria and also into Ehrlich ascites mitochondria at reduced levels, but not into mitochondria from heart and brain. Nevertheless, the 57-kDa protein and a number of proteins of less than 45 kDa are transported efficiently by all of the mitochondrial types studied. These results provide evidence for tissue- or cell-specific selectivity at the mitochondrial membrane level for the transport of some proteins. The transports of 63- and 57-kDa proteins are differentially inhibited by mouse liver mitochondrial matrix and membrane fractions, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Biochemistryjournal_title
Biochemistryauthors
Bhat NK,Avadhani NGdoi
10.1021/bi00348a041subject
Has Abstractpub_date
1985-12-31 00:00:00pages
8107-13issue
27eissn
0006-2960issn
1520-4995journal_volume
24pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::A structural understanding of the nature and scope of serpin inhibition mechanisms has been limited by the inability so far to crystallize any serpin-proteinase complex. We describe here the application of [(1)H-(15)N]-HSQC NMR on uniformly and residue-selectively (15)N-labeled serpin alpha(1)-proteinase inhibitor (Pi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001152+
更新日期:2000-10-03 00:00:00
abstract::Periodate-oxidized ATP (o-ATP) was prepared as an affinity label of nucleotide binding sites on the chloroform-released ox heart mitochondrial ATPase. In the presence of MgSO4, o-ATP is a substrate for the ATPase. It can act as a reversible, competitive inhibitor of ATPase activity and can also induce an irreversible ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00260a025
更新日期:1982-08-17 00:00:00
abstract::Fructose-1,6-bisphosphate muscle aldolase is an essential glycolytic enzyme that catalyzes reversible carbon-carbon bond formation by cleaving fructose 1,6-bisphosphate to yield dihydroxyacetone phosphate (DHAP) and d-glyceraldehyde phosphate. To elucidate the mechanistic role of conserved amino acid Asp-33, Asn-33 an...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi8021558
更新日期:2009-06-02 00:00:00
abstract::By limited proteolysis of a type kappa immunoglobulin light chain (Oku) with clostripain, both the VL and CL fragments were obtained with a high yield. The unfolding and refolding by guanidine hydrochloride of light chain Oku and its VL and CL fragments were studied at pH 7.5 and 25 degrees C with circular dichroism a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00393a015
更新日期:1987-09-22 00:00:00
abstract::Taurine/alpha-ketoglutarate (alphaKG) dioxygenase (TauD), an archetype alphaKG-dependent hydroxylase, is a non-heme mononuclear Fe(II) enzyme that couples the oxidative decarboxylation of alphaKG with the conversion of taurine to aminoacetaldehyde and sulfite. The crystal structure of taurine-alphaKG-Fe(II)TauD is kno...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi048746n
更新日期:2005-03-15 00:00:00
abstract::Whole tRNA preparation obtained from a human cell line (HT-29) of colon carcinoma and purified specific Escherichia coli tRNA were reacted with pyridoxal 5'-phosphate, reduced by sodium borohydride and digested with RNase A and snake venom phosphodiesterase. Two-dimensional chromatography of the pyridoxal 5'-phosphate...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00635a033
更新日期:1977-08-09 00:00:00
abstract::Human prolactin (hPRL) binds two human prolactin receptor molecules, creating active heterotrimeric complexes. Receptors bind dissimilar hormone surfaces termed site 1 and site 2 in an obligate ordered process. We sought to map the functional epitopes in site 1 of hPRL. Extensive alanine mutagenesis (102 of the 199 re...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi101838s
更新日期:2011-03-01 00:00:00
abstract::The electron spin resonance (ESR) spectra from spin-labeled phospholipids in recombinants of myelin proteolipid apoprotein with dimyristoylphosphatidylcholine have been simulated with the exchanged-coupled Bloch equations to obtain values for both the fraction of motionally restricted lipids and the exchange rate betw...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00401a009
更新日期:1988-01-12 00:00:00
abstract::In this paper the interaction of cytoplasmic CopZ and the N-terminal domain of the CopA ATPase from Bacillus subtilis has been studied by NMR through (15)N-(1)H HSQC experiments in order to understand the role of the two proteins in the whole copper trafficking mechanism of the bacteria. It appears that the two protei...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi027096p
更新日期:2003-02-25 00:00:00
abstract::The effect of tropomyosin alone on the binding of myosin subfragment 1 (S-1) to actin was compared to that of troponin-tropomyosin, with and without Ca2+. Over a range of ionic strength and with different ligands (adenyl-5'-yl imidodiphosphate, pyrophosphate, and adenosine 5'-diphosphate), tropomyosin confers slight c...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00280a027
更新日期:1983-05-24 00:00:00
abstract::Two hybrid ribulose-1,5 bisphosphate carboxylase/oxygenase (RubisCO) enzymes were constructed using RubisCO small subunit genes (rbcS) from two eucaryotic marine organisms, Cylindrotheca sp. N1 and Olisthodiscus luteus, cloned downstream of the RubisCO large subunit gene (rbcL) of the cyanobacterium Synechococcus PCC ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00139a018
更新日期:1992-06-23 00:00:00
abstract::Thymine DNA glycosylase (TDG) is a base excision repair enzyme with key functions in epigenetic regulation. Performing a critical step in a pathway for active DNA demethylation, TDG removes 5-formylcytosine and 5-carboxylcytosine, oxidized derivatives of 5-methylcytosine that are generated by TET (ten-eleven transloca...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00982
更新日期:2016-11-15 00:00:00
abstract::The dynamics of individual DNA molecules undergoing orthogonal field alternating gel electrophoresis (OFAGE) have been studied by use of T2 DNA molecules labeled with a dye and visualized with a fluorescence microscope. The mechanism of reorientation used by a molecule to align itself in the direction of the new ortho...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00465a036
更新日期:1990-04-03 00:00:00
abstract::Catalysis by glycogen phosphorylase involves a mechanism in which binding of one substrate tightens the binding of the other substrate to produce a productive ternary enzyme-substrate complex. In this work the molecular basis for this synergism is probed in crystallographic studies on ternary complexes in which the gl...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi960072w
更新日期:1996-06-11 00:00:00
abstract::The dimeric two-component system transmitter protein NRII (NtrB) of Escherichia coli, product of glnL (ntrB), controls transcription of nitrogen-regulated genes by catalyzing the phosphorylation and dephosphorylation of the transcription factor NRI (NtrC). Previous studies showed that the PII signal transduction prote...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi000794u
更新日期:2000-11-07 00:00:00
abstract::Deuterium NMR has been used to investigate the structure and dynamic state of cytochrome c complexed with bilayers of cardiolipin. Reductive methylation was employed to prepare [N epsilon, N epsilon-C2H3]lysyl cytochrome c, and deuterium exchange provided labeling of backbone sites to give [amide-2H]cytochrome c or mo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00230a010
更新日期:1991-04-23 00:00:00
abstract::Lysine-146 of rabbit muscle aldolase (D-fructose-1,6-biphosphate aldolase, EC 4.1.2.13) is absolutely conserved in class I (Schiff base) aldolases and has been implicated previously in catalysis by protein modification. Site-directed mutagenesis was used to change lysine-146 to alanine, glutamine, leucine, or histidin...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00206a036
更新日期:1994-10-11 00:00:00
abstract::Several peptides contained within the C-terminal sequence of the B2 subunit of Escherichia coli ribonucleotide reductase (RNR) were investigated for their ability to inhibit the enzyme, presumably by interfering with association of the B1 and B2 subunits. AcYLVGQIDSE, corresponding by sequence homology to a nonapeptid...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00247a008
更新日期:1991-08-20 00:00:00
abstract::Bacterial bioluminescence is a phenomenon resulting from the reaction of a two-component FMN-dependent aldehyde monooxygenase system, which comprises a bacterial luciferase and a flavin reductase. Bacterial luciferase (LuxAB) is one of the most extensively investigated two-component monooxygenases, while its reductase...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi1009985
更新日期:2010-11-02 00:00:00
abstract::Thermal pertubation difference spectra of sperm whale myoglobin (Mb) and soybean leghemoglobin a (Lb a) in the near-ultraviolet reveal similarities in the tryptophan environment of the two proteins. Of the two tryptophans in each protein, one has its indolyl NH group fully exposed to aqueous solvent, while the other b...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00620a009
更新日期:1977-01-11 00:00:00
abstract::The photochemical intermediate metarhodopsin II (meta II; lambda max = 380 nm) is generally identified with rho*, the conformation of photolyzed rhodopsin which binds and activates the visual G-protein, Gt [Emeis, D., & Hoffman, K.P. (1981) FEBS Lett. 136, 201-207]. Purified bovine rhodopsin was incorporated into vesi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00215a006
更新日期:1991-01-08 00:00:00
abstract::Flavin-dependent histone demethylases catalyze the posttranslational oxidative demethylation of mono- and dimethylated lysine residues, producing formaldehyde and hydrogen peroxide in addition to the corresponding demethylated protein. In vivo, histone demethylase LSD1 (KDM1; BCH110) is a component of the multiprotein...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi101776t
更新日期:2011-02-01 00:00:00
abstract::The tRNA(His) guanylyltransferase (Thg1) catalyzes the incorporation of a single guanosine residue at the -1 position (G(-1)) of tRNA(His), using an unusual 3'-5' nucleotidyl transfer reaction. Thg1 and Thg1 orthologs known as Thg1-like proteins (TLPs), which catalyze tRNA repair and editing, are the only known enzyme...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi201397f
更新日期:2012-01-10 00:00:00
abstract::Cyclobutane pyrimidine dimers (Pyr < > Pyr) are the major DNA photoproducts induced by the UV component of solar radiation. Photoreactivating enzyme (DNA photolyase) repairs DNA by utilizing the energy of visible light to break the cyclobutane ring of the dimer. Photolyases are monomeric proteins of 50-60 kDa with sto...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/bi00167a001
更新日期:1994-01-11 00:00:00
abstract::Oligopeptides and dipeptides are transported into Saccharomyces cerevisiae by a carrier-mediated system. In the dark, leucyl-p-nitroanilide (Leu-p-NA) and leucyl-leucyl-4-azido-2-nitrophenylalanine [Leu-Leu-Phe-(4N3,2NO2)] are competitive inhibitors of peptide transport by S. cerevisiae cells. The photolysis of yeast ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00266a037
更新日期:1982-11-09 00:00:00
abstract::Acyl carrier protein (ACP) is the central player in fatty acid (FA) biosynthesis. It covalently binds all FA intermediates and presents them to the enzymes needed for elongation. Bacterial ACP must interact with a large number of proteins, which raises the question of how different acyl-ACPs are recognized and disting...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi901713r
更新日期:2010-04-06 00:00:00
abstract::The inactivation of cytochrome P450 2B4 by aldehydes in a reconstituted enzyme system requires molecular oxygen and NADPH and is not prevented by the addition of catalase, superoxide dismutase, epoxide hydrolase, glutathione, or ascorbic acid. A strong correlation between loss of enzymatic activity and bleaching of th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9630568
更新日期:1997-04-22 00:00:00
abstract::Zn2+ is tetrahedrally bonded to the 4 nonadjacent thiols of each regulatory chain (Mr 17,000) near r-c contacts between catalytic (c) and regulatory chains (r) in aspartate transcarbamoylase (ATCase; c6r6). This paper reports on Zn2+ interactions with r dimer in the absence of stabilizing r-c contacts. After r2 and c3...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00480a019
更新日期:1990-07-17 00:00:00
abstract::Polyhistidine affinity tags are routinely employed as a convenient means of purifying recombinantly expressed proteins. A tacit assumption is commonly made that His tags have little influence on protein structure and function. Attachment of a His tag to the N-terminus of the robust globular protein myoglobin leads to ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi2003923
更新日期:2011-06-28 00:00:00
abstract::Vibrational frequencies associated with FeC and CO stretching and FeCO bending modes have been determined via resonance Raman (RR) and infrared (IR) spectroscopy for cytochrome c peroxidase (CCP) mutants prepared by site-directed mutagenesis. These include the bacterial "wild type", CCP(MI), and mutations involving gr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00415a015
更新日期:1988-07-26 00:00:00