Modification of ribonucleic acid by vitamin B6. 1. Specific interaction of pyridoxal 5'-phosphate with transfer ribonucleic acid.

abstract：:Calcium trapped within sonicated and resealed bovine rod outer segment disks is released upon light exposure with a stoichiometry of 0.75 +/- 0.05 calcium for each rhodopsin bleached. The amount of calcium liberated is proportional to the amount of bleaching in the range of 20 to 100% bleaching and is relatively insen...

journal_title：Biochemistry

authors： Smith HG Jr,Fager RS,Litman RJ

更新日期：1977-04-05 00:00:00

abstract：:The respiratory chain complexes can arrange into multienzyme assemblies, so-called supercomplexes. We present the first 3D map of a respiratory chain supercomplex. It was determined by random conical tilt electron microscopy analysis of a bovine supercomplex consisting of complex I, dimeric complex III, and complex IV...

journal_title：Biochemistry

authors： Schäfer E,Dencher NA,Vonck J,Parcej DN

更新日期：2007-11-06 00:00:00

abstract：:A steady-state kinetic analysis was conducted of the overall aminoacylation reaction catalyzed by isoleucyl-tRNA synthetase. The patterns of Lineweaver-Burk plots obtained indicated that tRNA adds to the enzyme only after isoleucyl adenylate formation and pyrophosphate release. These kinetic patterns were consistent w...

journal_title：Biochemistry

authors： Moe JG,Piszkiewicz D

更新日期：1979-06-26 00:00:00

abstract：:Stimulation of intracellular cholesterol esterification, which is catalyzed by the enzyme acyl-CoA:cholesterol O-acyltransferase (ACAT), by atherogenic lipoproteins in macrophages is a key step in the development of atheroma foam cells. Since other aspects of intracellular cholesterol metabolism involve proteolytic re...

journal_title：Biochemistry

authors： Schissel SL,Beatini N,Zha X,Maxfield FR,Tabas I

更新日期：1995-08-22 00:00:00

abstract：:An equimolar mixture of DL-[alpha-2H]- and DL-[alpha-13C]mandelate, when incubated with mandelate racemase (EC 5.1.2.2), shows conversion of singly labeled mandelate to unlabeled mandelate, due to solvent exchange of the alpha proton, while the level of doubly labeled mandelate remains at a constant low level. Similar...

journal_title：Biochemistry

authors： Sharp TR,Hegeman GD,Kenyon GL

更新日期：1977-03-22 00:00:00

abstract：:The structure of a mutant Escherichia coli thioredoxin with a glutamic acid substituted for a conserved lysine at position 36 adjacent to the active site has been solved using molecular replacement and refined at 2.0-A resolution to a crystallographic residual of 19.9%. The mutant was crystallized in an orthorhombic s...

journal_title：Biochemistry

authors： Nikkola M,Gleason FK,Fuchs JA,Eklund H

更新日期：1993-05-18 00:00:00

abstract：:Perturbations within the transactivation and carboxy-terminal domains of HIV-1 Rev were examined for effects on Rev responsive element (RRE) binding activities in vitro and biological activity in vivo. Binding affinities, specificities, and multimerization of the transactivation mutants M10 and Rev/Rex M10-16 on the R...

journal_title：Biochemistry

authors： Daly TJ,Rennert P,Lynch P,Barry JK,Dundas M,Rusche JR,Doten RC,Auer M,Farrington GK

更新日期：1993-08-31 00:00:00

abstract：:Many bacteria produce isonitrile-containing natural products that are derived from aromatic amino acids. The synthetic clusters that control biosynthesis most commonly encode two enzymes, designated PvcA and PvcB, as well as additional enzymes that direct synthesis of the natural product. The PvcA enzyme installs the ...

journal_title：Biochemistry

authors： Zhu J,Lippa GM,Gulick AM,Tipton PA

更新日期：2015-04-28 00:00:00

abstract：:A new strategy for the assignment of nonexchangeable proton resonances in oligonucleotide duplexes is presented and used to interpret the spectra of the oligonucleotide helix d(CpGpCpGpCpG) X d(CpGpCpGpCpG) in low salt (B form). This procedure is based on the use of sequential homodecoupling (1D) or COSY (2D) for the ...

journal_title：Biochemistry

authors： Frechet D,Cheng DM,Kan LS,Ts'o PO

更新日期：1983-10-25 00:00:00

abstract：:The thermal dependence of the structural stability of membrane-reconstituted yeast cytochrome c oxidase has been studied by using different techniques including high-sensitivity differential scanning calorimetry, differential detergent solubility thermal gel analysis, and enzyme activity measurements. For these studie...

journal_title：Biochemistry

authors： Morin PE,Diggs D,Freire E

更新日期：1990-01-23 00:00:00

abstract：:The mechanism of N-methyltryptophan oxidase, a flavin-dependent amine oxidase from Escherichia coli, was studied using a combination of kinetic isotope effects and theoretical calculations. The 15(kcat/Km) kinetic isotope effect for sarcosine oxidation is pH-dependent with a limiting value of 0.994-0.995 at high pH. D...

journal_title：Biochemistry

authors： Ralph EC,Hirschi JS,Anderson MA,Cleland WW,Singleton DA,Fitzpatrick PF

更新日期：2007-06-26 00:00:00

abstract：:An amino-terminal deletion mutant (residues 1-43) of human apolipoprotein A-I (apo hA-I) has been produced from a bacterial expression system to explore the structural and functional role of these amino acids, encoded by exon 3, in apo hA-I. Lipid binding of apo delta (1-43)A-I and lipid binding of apo hA-I are very s...

journal_title：Biochemistry

authors： Rogers DP,Brouillette CG,Engler JA,Tendian SW,Roberts L,Mishra VK,Anantharamaiah GM,Lund-Katz S,Phillips MC,Ray MJ

更新日期：1997-01-14 00:00:00

abstract：:Ammonia is an inhibitor of water oxidation and a structural analogue for substrate water, making it a valuable probe for the structural properties of the possible substrate-binding site on the oxygen-evolving complex (OEC) in photosystem II (PSII). By using the NH(3)-induced upshift of the 1365 cm(-)(1) IR mode in the...

journal_title：Biochemistry

authors： Fang CH,Chiang KA,Hung CH,Chang K,Ke SC,Chu HA

更新日期：2005-07-19 00:00:00

abstract：:The sensor kinase CitA and the response regulator CitB of Klebsiella pneumoniae form the paradigm of a subfamily of bacterial two-component regulatory systems that are capable of sensing tri- or dicarboxylates in the environment and then induce transporters for the uptake of these compounds. We recently showed that th...

journal_title：Biochemistry

authors： Gerharz T,Reinelt S,Kaspar S,Scapozza L,Bott M

更新日期：2003-05-20 00:00:00

abstract：:Staphylococcal fibronectin-binding protein (FnbA) is a surface-associated receptor responsible for the reversible binding of bacteria to human fibronectin and fibrin(ogen). Recently we have shown that FnbA serves as a substrate for coagulation factor XIIIa and undergoes covalent cross-linking to its ligands, resulting...

journal_title：Biochemistry

authors： Anderson ET,Fletcher L,Severin A,Murphy E,Baker SM,Matsuka YV

更新日期：2004-09-21 00:00:00

abstract：:The rate of the reaction between p-nitroperoxybenzoic acid and cytochrome c peroxidase (CcP) has been investigated as a function of pH and ionic strength. The pH dependence of the reaction between CcP and peracetic acid has also been determined. The rate of the reactions are influenced by two heme-linked ionizations i...

journal_title：Biochemistry

authors： Palamakumbura AH,Foshay MC,Vitello LB,Erman JE

更新日期：1999-11-23 00:00:00

abstract：:The enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) catalyzes the reaction between shikimate 3-phosphate and phosphoenolpyruvate to form 5-enolpyruvylshikimate 3-phosphate, an intermediate in the shikimate pathway, which leads to the biosynthesis of aromatic amino acids. EPSPS exists in an open conformation...

journal_title：Biochemistry

authors： Marques MR,Vaso A,Neto JR,Fossey MA,Oliveira JS,Basso LA,dos Santos DS,de Azevedo Junior WF,Palma MS

更新日期：2008-07-15 00:00:00

abstract：:6-Hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK) catalyzes the pyrophosphoryl transfer from ATP to 6-hydroxymethyl-7,8-dihydropterin (HP), the first reaction in the folate biosynthetic pathway. Arginine residues 82 and 92, strictly conserved in 35 HPPK sequences, play dynamic roles in the catalytic cycle of ...

journal_title：Biochemistry

authors： Blaszczyk J,Li Y,Shi G,Yan H,Ji X

更新日期：2003-02-18 00:00:00

abstract：:The existence of temperature optima in enzyme catalysis that occur before protein melting sets in can be described by different types of kinetic models. Such optima cause distinctly curved Arrhenius plots and have, for example, been observed in several cold-adapted enzymes from psychrophilic species. The two main expl...

journal_title：Biochemistry

authors： Åqvist J,Sočan J,Purg M

更新日期：2020-10-13 00:00:00

abstract：:PEP mutase catalyzes the conversion of phosphoenolpyruvate (PEP) to phosphonopyruvate in biosynthetic pathways leading to phosphonate secondary metabolites. A recent X-ray structure [Huang, K., Li, Z., Jia, Y., Dunaway-Mariano, D., and Herzberg, O. (1999) Structure (in press)] of the Mytilus edulis enzyme complexed wi...

journal_title：Biochemistry

authors： Jia Y,Lu Z,Huang K,Herzberg O,Dunaway-Mariano D

更新日期：1999-10-26 00:00:00

abstract：:Prion diseases are associated with a conformational switch in the prion protein (PrP) from its normal cellular form (denoted PrP(C)) to a disease-associated "scrapie" form (PrP(Sc)). A number of PrP(Sc)-like conformations can be generated by incubating recombinant PrP(C) at low pH, indicating that protonation of key r...

journal_title：Biochemistry

authors： Hosszu LL,Tattum MH,Jones S,Trevitt CR,Wells MA,Waltho JP,Collinge J,Jackson GS,Clarke AR

更新日期：2010-10-12 00:00:00

abstract：:The gene encoding for the methionyl aminopeptidase from the hyperthermophilic archaeon Pyrococcus furiosus (PfMetAP-II; EC 3.4.11.18) has been inserted into a pET 27b(+) vector and overexpressed in Escherichia coli. The new expression system resulted in a 5-fold increase in purified enzyme obtained from a 5 L fermento...

journal_title：Biochemistry

authors： Meng L,Ruebush S,D'souza VM,Copik AJ,Tsunasawa S,Holz RC

更新日期：2002-06-11 00:00:00

abstract：:One of the most promising approaches to anti-hepatitis C virus drug discovery is the development of inhibitors of the virally encoded protease NS3. This chymotrypsin-like serine protease is essential for the maturation of the viral polyprotein, and processing requires complex formation between NS3 and its cofactor NS4...

journal_title：Biochemistry

authors： Bianchi E,Orrù S,Dal Piaz F,Ingenito R,Casbarra A,Biasiol G,Koch U,Pucci P,Pessi A

更新日期：1999-10-19 00:00:00

abstract：:Recently, we described the formation of a complex between liposomes and the unfolded protein rhodanese (thiosulfate:cyanide sulfurtransferase, EC 2.8.1.1), which could be liberated and efficiently reactivated after treatment of the complex with detergents [Zardeneta, G., & Horowitz, P. M. (1992) Eur. J. Biochem. 210, ...

journal_title：Biochemistry

authors： Zardeneta G,Horowitz PM

更新日期：1993-12-21 00:00:00

abstract：:3-Fluorooxalacetate is a substrate for malic dehydrogenase. When enzymatic reduction is slower than the rate of epimerization of the two enantiomers, only (2R,3R)-erythro-fluoromalate is formed. Conversely, when a high enzyme level and excess of NADH lead to reduction that is fast relative to the epimerization rate, e...

journal_title：Biochemistry

authors： Urbauer JL,Bradshaw DE,Cleland WW

更新日期：1998-12-22 00:00:00

abstract：:We have investigated the denaturation by guanidine hydrochloride (Gdn X HCl), temperature, and pH of hemoglobin beta subunits and of the peptides beta (1-146), beta (56-146), and beta (1-55). The last peptide was insensitive to all of the three agents. In the other polypeptides denaturation by Gdn X HCl and temperatur...

journal_title：Biochemistry

authors： Franchi D,Fronticelli C,Bucci E

更新日期：1982-11-23 00:00:00

abstract：:Frequency-resolved fluorescence measurements have been performed to quantitate the lateral stress of the lipid layer containing nonbilayer phase preferring dioleoylphosphatidylethanolamine (DOPE). On the basis of a new rotational diffusion model, the wobbling diffusion constant (Dw), the curvature-related hopping diff...

journal_title：Biochemistry

authors： Chen SY,Cheng KH,Van der Meer BW

更新日期：1992-04-21 00:00:00

abstract：:Picosecond transient absorption spectra of rhodopsin and isorhodopsin were measured at room temperature with a double-beam laser spectrophotometer after excitation at 355 nm. Photolysis studies were performed on rhodopsin solubilized in two different detergents (digitonin and 3-[(3-cholamidopropyl)dimethylammonio]-1-p...

journal_title：Biochemistry

authors： Rudzki JE,Peters KS

更新日期：1984-08-14 00:00:00

abstract：:Di-leucine motifs have been implicated in the internalization or degradation of many membrane proteins. The epidermal growth factor receptor (EGFR) contains two di-leucine residues at 658 (TLRRLLQER) and 679 (NQALLRIL). To determine the role of these di-leucine motifs in regulating EGF receptor expression, activity, o...

journal_title：Biochemistry

authors： Morrison P,Chung KC,Rosner MR

更新日期：1996-11-19 00:00:00

abstract：:Thiocyanate reacts noncatalytically with myeloperoxidase-derived HOCl to produce hypothiocyanite (OSCN(-)), thereby potentially limiting the propensity of HOCl to inflict host tissue damage that can lead to inflammatory diseases. However, the efficiency with which SCN(-) captures HOCl in vivo depends on the concentrat...

journal_title：Biochemistry

authors： Xulu BA,Ashby MT

更新日期：2010-03-09 00:00:00