Abstract:
:Cyclobutane pyrimidine dimers (Pyr < > Pyr) are the major DNA photoproducts induced by the UV component of solar radiation. Photoreactivating enzyme (DNA photolyase) repairs DNA by utilizing the energy of visible light to break the cyclobutane ring of the dimer. Photolyases are monomeric proteins of 50-60 kDa with stoichiometric amounts of two noncovalent chromophore/cofactors. One of these cofactors is FADH-, and the second chromophore is either methenyltetrahydrofolate (MTHF) or 8-hydroxy-5-deazariboflavin (8-HDF). The enzyme binds the DNA substrate in a light-independent reaction, the second chromophore of the bound enzyme absorbs a visible photon and, by dipole-dipole interaction, transfers energy to FADH- which, in turn, transfers an electron to Pyr < > Pyr in DNA; the Pyr < > Pyr- splits and back electron transfer restores the dipyrimidine and the functional form of flavin ready for a new cycle of catalysis.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Sancar Adoi
10.1021/bi00167a001subject
Has Abstractpub_date
1994-01-11 00:00:00pages
2-9issue
1eissn
0006-2960issn
1520-4995journal_volume
33pub_type
杂志文章,评审相关文献
BIOCHEMISTRY文献大全abstract::An empirical preduction algorithm which uses information on the shrs on either side) interactions in 20 proteins to assign every residue in a protein to one of five conformational states is described. The conformational states are defined in terms of the backbone dihedral angles of the residue so that the prediction a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00668a030
更新日期:1976-11-16 00:00:00
abstract::To investigate cytosine protonation and its influence on the sequence-dependent thermal stability of DNA triplexes in detail, we have employed homo- and heteronuclear NMR experiments on specifically (15)N-labeled oligodeoxynucleotides that were designed to fold into intramolecular triple helices of the pyrimidine moti...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi992630n
更新日期:2000-05-16 00:00:00
abstract::Horseradish peroxidase (HRP) was oxidized by IrCl6(2-) to a mixture of compounds I and II, the rate of oxidation and the ratio of the mixture being greatly affected by pH (Hayashi & Yamazaki, 1979). Oxidation of HRP by IrCl6(2-) in the presence of fluoride was significantly accelerated. This resulted in the formation ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00046a003
更新日期:1995-11-21 00:00:00
abstract::A cyclic nucleotide phosphodiesterase was extensively purified from the 100000g supernatant fraction of human platelets. The purification was 2500-3000-fold with 30% recovery of activity. The enzyme was isolated by DEAE-cellulose chromatography followed by adsorption to blue dextran-Sepharose and elution with cAMP. Th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00303a034
更新日期:1984-04-10 00:00:00
abstract::Steady-state kinetic parameters were determined for the human leukocyte elastase catalyzed hydrolysis of a series of peptide-based thiobenzyl esters and p-nitroanilides. The peptide units are MeOSuc-Val, MeOSuc-Alan-Pro-Val (n = 0-2), and MeOSuc-Alan-Pro-Ala (n = 1 or 2). The results of this study suggest five importa...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00379a015
更新日期:1987-03-10 00:00:00
abstract::The extrinsic 33-kDa protein (P33) was cross-linked covalently to the binding site on P33-depleted PSII preparations which is responsible for reconstitution of photosynthetic water oxidation after PSII preparations have been washed with 1 M CaCl2. Conditions were found in which more than half of the cross-linked prote...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00439a033
更新日期:1989-06-27 00:00:00
abstract::Interaction of the peptide antibiotic alamethicin with phospholipid vesicles has been monitored by changes in its circular dichroic and fluorescent properties. The data are consistent with an incorporation of the peptide in the lipid bilayer. Aggregation of alamethicin in the membrane phase is evident from a character...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00384a015
更新日期:1987-05-19 00:00:00
abstract::Matrix metalloproteinases (MMPs) are a family of enzymes that are up-regulated in many diseases, including osteoarthritis (OA) and rheumatoid arthritis (RA). Here we report on a novel technique that can be used to simultaneously measure activity levels for a panel of enzymes, such as the MMPs. The technique, termed th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi036063m
更新日期:2004-03-23 00:00:00
abstract::The infectious isoform of the prion protein (PrPSc) is derived from cellular PrP (PrPC) in a conversion reaction involving a dramatic reorganization of secondary and tertiary structure. While our understanding of the pathogenic role of PrPSc has grown, the normal physiologic function of PrPC still remains unclear. Usi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972827k
更新日期:1998-05-19 00:00:00
abstract::Cyclopropane fatty acid (CFA) synthase of Escherichia coli catalyzes a modification of the acyl chains of phospholipid bilayers. We report (i) identification of the CFA synthase protein, (ii) overproduction (> 600-fold) and purification to essential homogeneity of the enzyme, and (iii) the amino acid sequence of CFA s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00160a011
更新日期:1992-11-17 00:00:00
abstract::The effects of incubation of erythrocyte ghosts under various conditions (ionic strength or addition of ankyrin, diamines, or ATP) on the lateral motion of band 3 in the membranes were studied by using the fluorescence photobleaching recovery technique. Incubation of ghosts with exogenous ankyrin increased the immobil...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00368a045
更新日期:1986-10-07 00:00:00
abstract::Two antisera used in the radioimmunoassay for human fibrinopeptide A (FPA) which appear to have different immunochemical specificities have been tested for cross-reactivity with fibrinogen and with three fragments of fibrinogen which contain the FPA sequence. These fragments were the three-chain, NH2-terminal disulfid...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00651a004
更新日期:1976-03-23 00:00:00
abstract::The effect of DNA supercoiling on a sterically very demanding threading intercalation process is investigated here. We find that the threading rate of a dimeric ruthenium complex into a negatively supercoiled plasmid at low binding density is 2 orders of magnitude higher than into the cleaved linear form. Further satu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi802336p
更新日期:2009-02-24 00:00:00
abstract::Malate dehydrogenase occurs in virtually all eucaryotic cells in mitochondrial and cytoplasmic forms, both of which are composed of two identical subunits. The reactivation of the mitochondrial isoenzyme has been the subject of previous studies [Jaenicke, R., Rudolph, R., & Heider, I. (1979) Biochemistry 18, 1217-1223...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00355a033
更新日期:1986-04-08 00:00:00
abstract::The Wilson disease copper-transporting ATPase plays a critical role in the intracellular trafficking of copper. Mutations in this protein lead to the accumulation of a toxic level of copper in the liver, kidney, and brain followed by extensive tissue damage and death. The ATPase has a novel amino-terminal domain ( app...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi992222j
更新日期:2000-02-22 00:00:00
abstract::Epigallocatechin-3-gallate (EGCG) is a catechin found in green tea that can inhibit the amyloid formation of a wide variety of proteins. EGCG's ability to prevent or redirect the amyloid formation of so many proteins may reflect a common mechanism of action, and thus, greater molecular-level insight into how it exerts...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.0c00043
更新日期:2020-03-17 00:00:00
abstract::A new fluorescent lipid analogue, bimanephosphatidylcholine, has been synthesized for use in lipid bilayers. This probe is well suited as an energy-transfer donor with N-(7-nitro-2,1,3-benzoxadiazol-4-yl)phosphatidylethanolamine as the acceptor. Dimyristoylphosphatidylethanolamine vesicles are prepared by sonication a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00330a014
更新日期:1985-04-23 00:00:00
abstract::The activity of peptidyl-tRNALys-CpCp2'dA was measured in an in vitro poly(A)-dependent polypeptide synthesizing system derived from Escherichia coli. It has already been shown that Lys-tRNALys-CpCp2'dA is active as an acceptor and Ac2-Lys-tRNALys-Cp2'dA can donate its peptidyl residue but that the overall poly(A)-dep...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00270a013
更新日期:1983-01-04 00:00:00
abstract::Sedimentation equilibrium and sedimentation velocity measurements demonstrate that turkey gizzard caldesmon is an elongated molecule of molecular mass 75 +/- 2 kDa. The frictional ratio (2.14) is consistent with a prolate ellipsoid of axial ratio 24, corresponding to an apparent length and width of 516 and 21.5 A, res...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00446a039
更新日期:1989-10-03 00:00:00
abstract::The beta-glycosidase from the hyperthermophilic Archaeon Sulfolobus solfataricus hydrolyzes beta-glycosides following a retaining mechanism based upon the action of two amino acids: Glu387, which acts as the nucleophile of the reaction, and Glu206, which acts as the general acid/base catalyst. The activities of inacti...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981855f
更新日期:1998-12-08 00:00:00
abstract::DNA polymerase delta from calf thymus has been purified to apparent homogeneity by a new procedure which utilizes hydrophobic interaction chromatography with phenyl-Sepharose at an early step to separate most of the calcium-dependent protease activity from DNA polymerase delta and alpha. The purified enzyme migrates a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00304a003
更新日期:1984-04-24 00:00:00
abstract::Actin depolymerizing factor (ADF)/cofilin enhances turnover of actin filaments by severing and depolymerizing filaments. A number of proteins functionally interact with ADF/cofilin to modulate the dynamics of actin filaments. Actin-interacting protein 1 (AIP1) has emerged as a conserved WD-repeat protein that specific...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/bi034600x
更新日期:2003-11-25 00:00:00
abstract::Restrictocin and related fungal endoribonucleases from the α-sarcin family site-specifically cleave the sarcin/ricin loop (SRL) on the ribosome to inhibit translation and ultimately trigger cell death. Previous studies showed that the SRL folds into a bulged-G motif and tetraloop, with restrictocin achieving a specifi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi1018336
更新日期:2011-04-12 00:00:00
abstract::Superoxide reductase (SOR) is an Fe protein that catalyzes the reduction of superoxide to give H(2)O(2). Recently, the mutation of the Glu47 residue into alanine (E47A) in the active site of SOR from Desulfoarculus baarsii has allowed the stabilization of an iron-peroxo species when quickly reacted with H(2)O(2) [Math...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0498151
更新日期:2004-07-13 00:00:00
abstract::Synaptotagmin 1 (syt1) is a synaptic vesicle-anchored membrane protein that acts as the calcium sensor for the synchronous component of neuronal exocytosis. Using site-directed spin labeling, the position and membrane interactions of a fragment of syt1 containing its two C2 domains (syt1C2AB) were assessed in bilayers...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200049c
更新日期:2011-04-05 00:00:00
abstract::Alzheimer's disease (AD) paired helical filaments (PHFs), building blocks of neurofibrillary tangles (NFTs) are composed of hyperphosphorylated forms of the microtubule-associated protein tau (i.e., PHF-tau). Currently, much effort is devoted to the development of diagnostic antibodies specific for PHF-tau since eleva...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi970380+
更新日期:1997-07-01 00:00:00
abstract::A new specific voltage-sensitive calcium channel (VSCC) blocker has been isolated from the venom of the fish-hunting cone snail Conus consors. This peptide, named omega-Ctx CNVIIA, consists of 27 amino acid residues folded by 3 disulfide bridges. Interestingly, loop 4, which is supposed to be crucial for selectivity, ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi002871r
更新日期:2001-12-04 00:00:00
abstract::Our recently reported pressure-jump relaxation kinetics experiments on staphylococcal nuclease folding and unfolding [Vidugiris et al. (1995) Biochemistry 34, 4909] demonstrated that both transitions exhibit positive activation volumes, with that of folding being much larger than that of unfolding. Thus high pressure ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952012g
更新日期:1996-03-26 00:00:00
abstract::Tropomyosins from bovine aorta and pulmonary artery exhibit identical electrophoretic patterns in sodium dodecyl sulfate but differ from tropomyosins of either chicken gizzard or rabbit skeletal muscle. Each of the four tropomyosins binds readily to skeletal muscle F-actin as indicated by their sedimentation with acti...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00299a029
更新日期:1984-02-14 00:00:00
abstract::The unique occurrence of G-quadruplexes in the AT-rich genome of human malaria parasite Plasmodium falciparum provides hints about their critical roles in parasite survival, pathogenesis, and host immune evasion. An intriguing question is whether these noncanonical structures can serve as molecular targets for small m...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00964
更新日期:2017-12-26 00:00:00