Characterization of two major neutral glyceroglucolipids of the human gastric content.

Abstract:

:Two major neutral glyceroglucolipids (A and B) have been isolated from lipid extract of human gastric content by the procedure involving column fractionation on DEAE-Sephadex, silicic acid, and thin-layer chromatography. Both glycolipids contained glucose, glyceryl ethers, and fatty acids. The structures of these glycolipids were identified by mild alkaline methanolysis, oxidation with periodate and chromium trioxide, and permethylation studies. Based on the obtained data, we propose that glycolipid A is a monoalkylmonoacylglyceryl hexaglucoside and glycolipid B is a monoalkylmonoacylglyceryl octaglucoside. The diglyceride portion of these glycolipids consists mostly of 1-O-alkyl-2-O-acylglycerol.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Slomiany BL,Slomiany A,Glass GB

doi

10.1021/bi00637a003

subject

Has Abstract

pub_date

1977-09-06 00:00:00

pages

3954-8

issue

18

eissn

0006-2960

issn

1520-4995

journal_volume

16

pub_type

杂志文章
  • Redefinition of the carbohydrate specificity of Erythrina corallodendron lectin based on solid-phase binding assays and molecular modeling of native and recombinant forms obtained by site-directed mutagenesis.

    abstract::Binding of the N-acetyllactosamine-specific lectin from Erythrina corallodendron (ECorL) to four glycosphingolipids has been tested using the microtiter well assay. The role of several amino acids in the binding site region was studied by combining binding assays and molecular modeling for native and recombinant forms...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi962231h

    authors: Moreno E,Teneberg S,Adar R,Sharon N,Karlsson KA,Angström J

    更新日期:1997-04-15 00:00:00

  • Unbinding of oxidized cytochrome c from photosynthetic reaction center of Rhodobacter sphaeroides is the bottleneck of fast turnover.

    abstract::To understand the details of rate limitation of turnover of the photosynthetic reaction center, photooxidation of horse heart cytochrome c by reaction center from Rhodobacter spheroides in detergent dispersion has been examined by intense continuous illumination under a wide variety of conditions of cytochrome concent...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi991563u

    authors: Gerencsér L,Laczkó G,Maróti P

    更新日期:1999-12-21 00:00:00

  • Two-site binding of beta-cyclodextrin to the Alzheimer Abeta(1-40) peptide measured with combined PFG-NMR diffusion and induced chemical shifts.

    abstract::The interactions of Alzheimer's amyloid beta-peptide with cyclodextrins were studied by (1)H NMR: the translational diffusion coefficient of the peptide and chemical shift changes were studied by the presence of variable concentrations of cyclodextrins. For the full-length peptide, Abeta(1-40), the combined results of...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi036254p

    authors: Danielsson J,Jarvet J,Damberg P,Gräslund A

    更新日期:2004-05-25 00:00:00

  • Distance between the visible copper and cytochrome a in bovine heart cytochrome oxidase.

    abstract::Electron paramagnetic resonance (EPR) at 15 K was used to probe the magnetic interaction between the visible copper CuA2+ and ferric cytochrome a in the carbon monoxide compound of beef heart cytochrome oxidase. At pH 8.6, the midpoint potentials (Em's) for one-electron oxidation of CuA+ and cytochrome a2+ were found ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00330a028

    authors: Goodman G,Leigh JS Jr

    更新日期:1985-04-23 00:00:00

  • Effect of solvent diffusion on the apomyoglobin-water interface.

    abstract::Few techniques can identify interactions between proteins and individual water molecules when the protein is in solution. The present work has sought to bridge the gap between the molecular level studies and the search for a physical property of the solution (bathing the proteins) that would regulate the protein hydra...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi012210+

    authors: Twist C,Royer C,Alpert B

    更新日期:2002-08-13 00:00:00

  • Mechanism of bacteriophage T4 DNA holoenzyme assembly: the 44/62 protein acts as a molecular motor.

    abstract::The role of ATP hydrolysis by the 44/62 protein in formation of the stable holoenzyme DNA replication complex has been further elucidated by specifically examining the role that the 44/62 protein plays in loading the 45 protein onto the DNA substrate. A stable phospho-45 protein or phosphorylated holoenzyme complex wa...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi962139l

    authors: Berdis AJ,Benkovic SJ

    更新日期:1997-03-11 00:00:00

  • Structural insight into how Streptomyces coelicolor maltosyl transferase GlgE binds α-maltose 1-phosphate and forms a maltosyl-enzyme intermediate.

    abstract::GlgE (EC 2.4.99.16) is an α-maltose 1-phosphate:(1→4)-α-d-glucan 4-α-d-maltosyltransferase of the CAZy glycoside hydrolase 13_3 family. It is the defining enzyme of a bacterial α-glucan biosynthetic pathway and is a genetically validated anti-tuberculosis target. It catalyzes the α-retaining transfer of maltosyl units...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi500183c

    authors: Syson K,Stevenson CE,Rashid AM,Saalbach G,Tang M,Tuukkanen A,Svergun DI,Withers SG,Lawson DM,Bornemann S

    更新日期:2014-04-22 00:00:00

  • Biogenesis of processing-competent secretory organelles in vitro.

    abstract::Propeptide processing occurs in specific compartments of the secretory pathway, but how these processing-competent organelles are generated from their processing-incompetent precursor compartments is unknown. To dissect the process biochemically, we have developed a novel cell-free system reconstituting the production...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi0112762

    authors: Andresen JM,Moore HP

    更新日期:2001-10-30 00:00:00

  • An alpha-helical burst in the src SH3 folding pathway.

    abstract::Src SH3 is a small all-beta-sheet protein composed of a single domain. We studied the folding behavior of src SH3 at various conditions by circular dichroism (CD), fluorescence, and X-ray solution scattering methods. On the src SH3 folding pathway, an alpha-helix-rich intermediate appeared not only at subzero temperat...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi0618262

    authors: Li J,Shinjo M,Matsumura Y,Morita M,Baker D,Ikeguchi M,Kihara H

    更新日期:2007-05-01 00:00:00

  • Subunit dissociation as a possible mechanism of glucocorticoid receptor activation.

    abstract::For the elucidation of the mechanism of steroid hormone receptor activation, the hydrodynamic properties of the unactivated and activated forms of the nonproteolyzed glucocorticoid receptor from the mouse AtT-20 pituitary tumor cell line were determined. The unactivated, molybdate-stabilized receptor has the following...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00277a038

    authors: Vedeckis WV

    更新日期:1983-04-12 00:00:00

  • Isolation and characterization of papaya peptidase A from commercial chymopapain.

    abstract::Chromatography on a column of SP-Sephadex shows that commercial chymopapain contains three components with proteolytic activity. Each behaves as a single protein upon rechromatography and electrophoresis on polyacrylamide gels. The major component, which represents 31% of the activity applied to the column and is the ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00687a028

    authors: Robinson GW

    更新日期:1975-08-12 00:00:00

  • The ice-binding site of sea raven antifreeze protein is distinct from the carbohydrate-binding site of the homologous C-type lectin.

    abstract::Antifreeze proteins lower the freezing point of their solution by binding to ice and inhibiting its growth. One of several structurally different antifreeze proteins in fishes (type II) is homologous to the carbohydrate-recognition domain of Ca2+-dependent lectins and adopts the same three-dimensional fold. Type II an...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi9820513

    authors: Loewen MC,Gronwald W,Sönnichsen FD,Sykes BD,Davies PL

    更新日期:1998-12-22 00:00:00

  • Role of arginine residue in saccharopine dehydrogenase (L-lysine forming) from baker's yeast.

    abstract::The baker's yeast saccharopine dehydrogenase (EC 1.5.1.7) was inactivated by 2,3-butanedione following pseudo-first-order reaction kinetics. The pseudo-first-order rate constant for inactivation was linearly related to the butanedione concentration, and a value of 7.5 M-1 min-1 was obtained for the second-order rate c...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00506a004

    authors: Fujioka M,Takata Y

    更新日期:1981-02-03 00:00:00

  • Orientation-conserved transfer of symmetric Krebs cycle intermediates in mammalian tissue.

    abstract::Metabolism of [2-13C]-, [3-13C]-, and [1,2,3-13C]propionate in perfused rat livers and [2-13C]-acetate in perfused rat hearts has been examined in tissue extracts by 13C NMR. Label from [2-13C]-propionate was preferentially incorporated into the C2 carbon of lactate, alanine, and aspartate in liver tissue while label ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00186a029

    authors: Sherry AD,Sumegi B,Miller B,Cottam GL,Gavva S,Jones JG,Malloy CR

    更新日期:1994-05-24 00:00:00

  • Modification of Escherichia coli ribosomes with the fluorescent reagent N-[[(iodoacetyl)amino]ethyl]-5-naphthylamine-1-sulfonic acid. Identification of derivatized L31' and studies on its intraribosomal properties.

    abstract::N-[[(Iodoacetyl)amino]ethyl]-5-naphthylamine-1-sulfonic acid (IAEDANS) is a fluorescent reagent which reacts covalently with the free thiol groups of proteins. When the reagent is reacted with the Escherichia coli ribosome under mild conditions, gel electrophoresis shows modification of predominantly two proteins, S18...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00346a042

    authors: Hanas JS,Simpson MV

    更新日期:1985-12-03 00:00:00

  • Structure of alpha-glycerophosphate oxidase from Streptococcus sp.: a template for the mitochondrial alpha-glycerophosphate dehydrogenase.

    abstract::The FAD-dependent alpha-glycerophosphate oxidase (GlpO) from Enterococcus casseliflavus and Streptococcus sp. was originally studied as a soluble flavoprotein oxidase; surprisingly, the GlpO sequence is 30-43% identical to those of the alpha-glycerophosphate dehydrogenases (GlpDs) from mitochondrial and bacterial sour...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi701685u

    authors: Colussi T,Parsonage D,Boles W,Matsuoka T,Mallett TC,Karplus PA,Claiborne A

    更新日期:2008-01-22 00:00:00

  • Effects of replacement of a double bond by a cyclopropane ring in phosphatidylethanolamines: a 2H NMR study of phase transitions and molecular organization.

    abstract::The thermotropic behavior and molecular properties of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) and 1-palmitoyl-2-dihydrosterculoyl-sn-glycero-3-phosphoethanolamine (PDSPE) have been investigated by 2H NMR spectroscopy using samples selectively labeled at the 5'-, 9'-, 10'-, and 16'-positions of the...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00325a038

    authors: Perly B,Smith IC,Jarrell HC

    更新日期:1985-02-12 00:00:00

  • The membrane-bound quinohemoprotein alcohol dehydrogenase from Gluconacetobacter diazotrophicus PAL5 carries a [2Fe-2S] cluster.

    abstract::Gluconacetobacter diazotrophicus stands out among the acetic acid bacteria as it fixes dinitrogen and is a true endophyte. It has a set of constitutive enzymes to oxidize ethanol and acetaldehyde which is upregulated during N(2)-dependent growth. The membrane-bound alcohol dehydrogenase (ADH) is a heterodimer (subunit...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi9015007

    authors: Gómez-Manzo S,Solano-Peralta A,Saucedo-Vázquez JP,Escamilla-Marván JE,Kroneck PM,Sosa-Torres ME

    更新日期:2010-03-23 00:00:00

  • Do the structures of big ET-1 and big ET-3 adopt a similar overall fold? Consequences for endothelin converting enzyme specificity.

    abstract::Big ET-1 and big ET-3 are precursor peptides which render endothelin-1 (ET-1) and endothelin-3 (ET-3) relatively unreactive and resistant to proteolytic cleavage. Big ET-1 is cleaved in vivo by ECE-1 (endothelin-converting enzyme), and big ET-3 is also cleaved but apparently to a significantly lesser extent by this en...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi981689b

    authors: Cronin NB,Wallace BA

    更新日期:1999-02-09 00:00:00

  • The hydroxyl of threonine 13 of the bovine 70-kDa heat shock cognate protein is essential for transducing the ATP-induced conformational change.

    abstract::The mechanism by which ATP binding transduces a conformational change in 70-kDa heat shock proteins that results in release of bound peptides remains obscure. Wei and Hendershot demonstrated that mutating Thr37 of hamster BiP to glycine impeded the ATP-induced conformational change, as monitored by proteolysis [(1995)...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi981510x

    authors: Sousa MC,McKay DB

    更新日期:1998-11-03 00:00:00

  • Structural and functional determinants of human plasma phospholipid transfer protein activity as revealed by site-directed mutagenesis of charged amino acids.

    abstract::Human plasma phospholipid transfer protein (PLTP) exchanges phospholipids between lipoproteins and remodels high-density lipoproteins (HDLs). We determined phospholipid transfer activity and HDL binding ability in wild-type PLTP and in 16 PLTP variants created by replacing 12 charged amino acids by site-directed mutag...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi027006g

    authors: Ponsin G,Qu SJ,Fan HZ,Pownall HJ

    更新日期:2003-04-22 00:00:00

  • Kinetics and thermodynamics of phalloidin binding to actin filaments from three divergent species.

    abstract::We compared the kinetics and thermodynamics of rhodamine phalloidin binding to actin purified from rabbit skeletal muscle, Acanthamoeba castellanii, and Saccharomyces cerevisiae in 50 mM KCl, 1 mM MgCl2, and pH 7.0 buffer at 22 degrees C. Filaments of S. cerevisiae actin bind rhodamine phalloidin more weakly than Acan...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi961047t

    authors: De La Cruz EM,Pollard TD

    更新日期:1996-11-12 00:00:00

  • Electrostatic evaluation of the signature motif (H/V)CX5R(S/T) in protein-tyrosine phosphatases.

    abstract::The catalytic activity of protein-tyrosine phosphatases (PTPs) is mediated by a cysteine side chain which carries out a nucleophilic attack initiating the phosphate cleavage. Experimentally, it has been observed that the active site cysteine has a remarkably low pKa. In the present study, we have investigated the orig...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi971187i

    authors: Peters GH,Frimurer TM,Olsen OH

    更新日期:1998-04-21 00:00:00

  • Structural and functional roles of the cysteine residues of Bacillus stearothermophilus farnesyl diphosphate synthase.

    abstract::p-(Chloromercuri)benzoic acid inhibited the catalytic activity of Bacillus stearothermophilus farnesyl diphosphate synthase (FPP synthase), which contains only two cysteine residues at positions 73 and 289. In order to explore the role of the cysteine residues, either or both of them were replaced with phenylalanine o...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00208a015

    authors: Koyama T,Obata S,Saito K,Takeshita-Koike A,Ogura K

    更新日期:1994-10-25 00:00:00

  • A C-truncated glutamyl-tRNA synthetase specific for tRNA(Glu) is stimulated by its free complementary distal domain: mechanistic and evolutionary implications.

    abstract::Faithful translation of the genetic code is mainly based on the specificity of tRNA aminoacylation catalyzed by aminoacyl-tRNA synthetases. These enzymes are comprised of a catalytic core and several appended domains. Bacterial glutamyl-tRNA synthetases (GluRS) contain five structural domains, the two distal ones inte...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi801690f

    authors: Dubois DY,Blais SP,Huot JL,Lapointe J

    更新日期:2009-06-30 00:00:00

  • 1H, 15N, 13C, and 13CO assignments of human interleukin-4 using three-dimensional double- and triple-resonance heteronuclear magnetic resonance spectroscopy.

    abstract::The assignment of the 1H, 15N, 13CO, and 13C resonances of recombinant human interleukin-4 (IL-4), a protein of 133 residues and molecular mass of 15.4 kDa, is presented based on a series of 11 three-dimensional (3D) double- and triple-resonance heteronuclear NMR experiments. These studies employ uniformly labeled 15N...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00132a026

    authors: Powers R,Garrett DS,March CJ,Frieden EA,Gronenborn AM,Clore GM

    更新日期:1992-05-05 00:00:00

  • Elastin binds to a multifunctional 67-kilodalton peripheral membrane protein.

    abstract::Elastin binding proteins from plasma membranes of elastin-producing cells were isolated by affinity chromatography on immobilized elastin peptides. Three proteins of 67, 61, and 55 kDa were released from the elastin resin by guanidine/detergent, soluble elastin peptides, synthetic peptide VGVAPG, or galactoside sugars...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00435a014

    authors: Mecham RP,Hinek A,Entwistle R,Wrenn DS,Griffin GL,Senior RM

    更新日期:1989-05-02 00:00:00

  • The role of Gly-4 of human cystatin A (stefin A) in the binding of target proteinases. Characterization by kinetic and equilibrium methods of the interactions of cystatin A Gly-4 mutants with papain, cathepsin B, and cathepsin L.

    abstract::The importance of the evolutionarily conserved Gly-4 residue for the affinity and kinetics of interaction of cystatin A with several cysteine proteinases was assessed by site-directed mutagenesis. Even the smallest replacement, by Ala, resulted in approximately 1000-, approximately 10- and approximately 6000-fold decr...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi980026r

    authors: Estrada S,Nycander M,Hill NJ,Craven CJ,Waltho JP,Björk I

    更新日期:1998-05-19 00:00:00

  • The DNA sequence of the human beta-globin region is strongly biased in favor of long strings of contiguous purine or pyrimidine residues.

    abstract::The DNA sequence of the human beta-globin region, comprising over 67 kilobase pairs, has been analyzed for the occurrence of strings of contiguous purine or pyrimidine residues. Tracts of 10 or more contiguous residues are found 4 times more frequently than would be expected with a random distribution of bases, so tha...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00398a050

    authors: Behe MJ

    更新日期:1987-12-01 00:00:00

  • Quantitative measurement of Ca(2+)-dependent calmodulin-target binding by Fura-2 and CFP and YFP FRET imaging in living cells.

    abstract::Calcium dynamics and its linked molecular interactions cause a variety of biological responses; thus, exploiting techniques for detecting both concurrently is essential. Here we describe a method for measuring the cytosolic Ca(2+) concentration ([Ca(2+)](i)) and protein-protein interactions within the same cell, using...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi200287x

    authors: Mori MX,Imai Y,Itsuki K,Inoue R

    更新日期:2011-05-31 00:00:00