Abstract:
:Two major neutral glyceroglucolipids (A and B) have been isolated from lipid extract of human gastric content by the procedure involving column fractionation on DEAE-Sephadex, silicic acid, and thin-layer chromatography. Both glycolipids contained glucose, glyceryl ethers, and fatty acids. The structures of these glycolipids were identified by mild alkaline methanolysis, oxidation with periodate and chromium trioxide, and permethylation studies. Based on the obtained data, we propose that glycolipid A is a monoalkylmonoacylglyceryl hexaglucoside and glycolipid B is a monoalkylmonoacylglyceryl octaglucoside. The diglyceride portion of these glycolipids consists mostly of 1-O-alkyl-2-O-acylglycerol.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Slomiany BL,Slomiany A,Glass GBdoi
10.1021/bi00637a003subject
Has Abstractpub_date
1977-09-06 00:00:00pages
3954-8issue
18eissn
0006-2960issn
1520-4995journal_volume
16pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Binding of the N-acetyllactosamine-specific lectin from Erythrina corallodendron (ECorL) to four glycosphingolipids has been tested using the microtiter well assay. The role of several amino acids in the binding site region was studied by combining binding assays and molecular modeling for native and recombinant forms...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi962231h
更新日期:1997-04-15 00:00:00
abstract::To understand the details of rate limitation of turnover of the photosynthetic reaction center, photooxidation of horse heart cytochrome c by reaction center from Rhodobacter spheroides in detergent dispersion has been examined by intense continuous illumination under a wide variety of conditions of cytochrome concent...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi991563u
更新日期:1999-12-21 00:00:00
abstract::The interactions of Alzheimer's amyloid beta-peptide with cyclodextrins were studied by (1)H NMR: the translational diffusion coefficient of the peptide and chemical shift changes were studied by the presence of variable concentrations of cyclodextrins. For the full-length peptide, Abeta(1-40), the combined results of...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi036254p
更新日期:2004-05-25 00:00:00
abstract::Electron paramagnetic resonance (EPR) at 15 K was used to probe the magnetic interaction between the visible copper CuA2+ and ferric cytochrome a in the carbon monoxide compound of beef heart cytochrome oxidase. At pH 8.6, the midpoint potentials (Em's) for one-electron oxidation of CuA+ and cytochrome a2+ were found ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00330a028
更新日期:1985-04-23 00:00:00
abstract::Few techniques can identify interactions between proteins and individual water molecules when the protein is in solution. The present work has sought to bridge the gap between the molecular level studies and the search for a physical property of the solution (bathing the proteins) that would regulate the protein hydra...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi012210+
更新日期:2002-08-13 00:00:00
abstract::The role of ATP hydrolysis by the 44/62 protein in formation of the stable holoenzyme DNA replication complex has been further elucidated by specifically examining the role that the 44/62 protein plays in loading the 45 protein onto the DNA substrate. A stable phospho-45 protein or phosphorylated holoenzyme complex wa...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi962139l
更新日期:1997-03-11 00:00:00
abstract::GlgE (EC 2.4.99.16) is an α-maltose 1-phosphate:(1→4)-α-d-glucan 4-α-d-maltosyltransferase of the CAZy glycoside hydrolase 13_3 family. It is the defining enzyme of a bacterial α-glucan biosynthetic pathway and is a genetically validated anti-tuberculosis target. It catalyzes the α-retaining transfer of maltosyl units...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500183c
更新日期:2014-04-22 00:00:00
abstract::Propeptide processing occurs in specific compartments of the secretory pathway, but how these processing-competent organelles are generated from their processing-incompetent precursor compartments is unknown. To dissect the process biochemically, we have developed a novel cell-free system reconstituting the production...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0112762
更新日期:2001-10-30 00:00:00
abstract::Src SH3 is a small all-beta-sheet protein composed of a single domain. We studied the folding behavior of src SH3 at various conditions by circular dichroism (CD), fluorescence, and X-ray solution scattering methods. On the src SH3 folding pathway, an alpha-helix-rich intermediate appeared not only at subzero temperat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0618262
更新日期:2007-05-01 00:00:00
abstract::For the elucidation of the mechanism of steroid hormone receptor activation, the hydrodynamic properties of the unactivated and activated forms of the nonproteolyzed glucocorticoid receptor from the mouse AtT-20 pituitary tumor cell line were determined. The unactivated, molybdate-stabilized receptor has the following...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00277a038
更新日期:1983-04-12 00:00:00
abstract::Chromatography on a column of SP-Sephadex shows that commercial chymopapain contains three components with proteolytic activity. Each behaves as a single protein upon rechromatography and electrophoresis on polyacrylamide gels. The major component, which represents 31% of the activity applied to the column and is the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00687a028
更新日期:1975-08-12 00:00:00
abstract::Antifreeze proteins lower the freezing point of their solution by binding to ice and inhibiting its growth. One of several structurally different antifreeze proteins in fishes (type II) is homologous to the carbohydrate-recognition domain of Ca2+-dependent lectins and adopts the same three-dimensional fold. Type II an...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9820513
更新日期:1998-12-22 00:00:00
abstract::The baker's yeast saccharopine dehydrogenase (EC 1.5.1.7) was inactivated by 2,3-butanedione following pseudo-first-order reaction kinetics. The pseudo-first-order rate constant for inactivation was linearly related to the butanedione concentration, and a value of 7.5 M-1 min-1 was obtained for the second-order rate c...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00506a004
更新日期:1981-02-03 00:00:00
abstract::Metabolism of [2-13C]-, [3-13C]-, and [1,2,3-13C]propionate in perfused rat livers and [2-13C]-acetate in perfused rat hearts has been examined in tissue extracts by 13C NMR. Label from [2-13C]-propionate was preferentially incorporated into the C2 carbon of lactate, alanine, and aspartate in liver tissue while label ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00186a029
更新日期:1994-05-24 00:00:00
abstract::N-[[(Iodoacetyl)amino]ethyl]-5-naphthylamine-1-sulfonic acid (IAEDANS) is a fluorescent reagent which reacts covalently with the free thiol groups of proteins. When the reagent is reacted with the Escherichia coli ribosome under mild conditions, gel electrophoresis shows modification of predominantly two proteins, S18...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00346a042
更新日期:1985-12-03 00:00:00
abstract::The FAD-dependent alpha-glycerophosphate oxidase (GlpO) from Enterococcus casseliflavus and Streptococcus sp. was originally studied as a soluble flavoprotein oxidase; surprisingly, the GlpO sequence is 30-43% identical to those of the alpha-glycerophosphate dehydrogenases (GlpDs) from mitochondrial and bacterial sour...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi701685u
更新日期:2008-01-22 00:00:00
abstract::The thermotropic behavior and molecular properties of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) and 1-palmitoyl-2-dihydrosterculoyl-sn-glycero-3-phosphoethanolamine (PDSPE) have been investigated by 2H NMR spectroscopy using samples selectively labeled at the 5'-, 9'-, 10'-, and 16'-positions of the...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00325a038
更新日期:1985-02-12 00:00:00
abstract::Gluconacetobacter diazotrophicus stands out among the acetic acid bacteria as it fixes dinitrogen and is a true endophyte. It has a set of constitutive enzymes to oxidize ethanol and acetaldehyde which is upregulated during N(2)-dependent growth. The membrane-bound alcohol dehydrogenase (ADH) is a heterodimer (subunit...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9015007
更新日期:2010-03-23 00:00:00
abstract::Big ET-1 and big ET-3 are precursor peptides which render endothelin-1 (ET-1) and endothelin-3 (ET-3) relatively unreactive and resistant to proteolytic cleavage. Big ET-1 is cleaved in vivo by ECE-1 (endothelin-converting enzyme), and big ET-3 is also cleaved but apparently to a significantly lesser extent by this en...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981689b
更新日期:1999-02-09 00:00:00
abstract::The mechanism by which ATP binding transduces a conformational change in 70-kDa heat shock proteins that results in release of bound peptides remains obscure. Wei and Hendershot demonstrated that mutating Thr37 of hamster BiP to glycine impeded the ATP-induced conformational change, as monitored by proteolysis [(1995)...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981510x
更新日期:1998-11-03 00:00:00
abstract::Human plasma phospholipid transfer protein (PLTP) exchanges phospholipids between lipoproteins and remodels high-density lipoproteins (HDLs). We determined phospholipid transfer activity and HDL binding ability in wild-type PLTP and in 16 PLTP variants created by replacing 12 charged amino acids by site-directed mutag...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi027006g
更新日期:2003-04-22 00:00:00
abstract::We compared the kinetics and thermodynamics of rhodamine phalloidin binding to actin purified from rabbit skeletal muscle, Acanthamoeba castellanii, and Saccharomyces cerevisiae in 50 mM KCl, 1 mM MgCl2, and pH 7.0 buffer at 22 degrees C. Filaments of S. cerevisiae actin bind rhodamine phalloidin more weakly than Acan...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961047t
更新日期:1996-11-12 00:00:00
abstract::The catalytic activity of protein-tyrosine phosphatases (PTPs) is mediated by a cysteine side chain which carries out a nucleophilic attack initiating the phosphate cleavage. Experimentally, it has been observed that the active site cysteine has a remarkably low pKa. In the present study, we have investigated the orig...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi971187i
更新日期:1998-04-21 00:00:00
abstract::p-(Chloromercuri)benzoic acid inhibited the catalytic activity of Bacillus stearothermophilus farnesyl diphosphate synthase (FPP synthase), which contains only two cysteine residues at positions 73 and 289. In order to explore the role of the cysteine residues, either or both of them were replaced with phenylalanine o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00208a015
更新日期:1994-10-25 00:00:00
abstract::Faithful translation of the genetic code is mainly based on the specificity of tRNA aminoacylation catalyzed by aminoacyl-tRNA synthetases. These enzymes are comprised of a catalytic core and several appended domains. Bacterial glutamyl-tRNA synthetases (GluRS) contain five structural domains, the two distal ones inte...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi801690f
更新日期:2009-06-30 00:00:00
abstract::The assignment of the 1H, 15N, 13CO, and 13C resonances of recombinant human interleukin-4 (IL-4), a protein of 133 residues and molecular mass of 15.4 kDa, is presented based on a series of 11 three-dimensional (3D) double- and triple-resonance heteronuclear NMR experiments. These studies employ uniformly labeled 15N...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00132a026
更新日期:1992-05-05 00:00:00
abstract::Elastin binding proteins from plasma membranes of elastin-producing cells were isolated by affinity chromatography on immobilized elastin peptides. Three proteins of 67, 61, and 55 kDa were released from the elastin resin by guanidine/detergent, soluble elastin peptides, synthetic peptide VGVAPG, or galactoside sugars...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00435a014
更新日期:1989-05-02 00:00:00
abstract::The importance of the evolutionarily conserved Gly-4 residue for the affinity and kinetics of interaction of cystatin A with several cysteine proteinases was assessed by site-directed mutagenesis. Even the smallest replacement, by Ala, resulted in approximately 1000-, approximately 10- and approximately 6000-fold decr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi980026r
更新日期:1998-05-19 00:00:00
abstract::The DNA sequence of the human beta-globin region, comprising over 67 kilobase pairs, has been analyzed for the occurrence of strings of contiguous purine or pyrimidine residues. Tracts of 10 or more contiguous residues are found 4 times more frequently than would be expected with a random distribution of bases, so tha...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00398a050
更新日期:1987-12-01 00:00:00
abstract::Calcium dynamics and its linked molecular interactions cause a variety of biological responses; thus, exploiting techniques for detecting both concurrently is essential. Here we describe a method for measuring the cytosolic Ca(2+) concentration ([Ca(2+)](i)) and protein-protein interactions within the same cell, using...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200287x
更新日期:2011-05-31 00:00:00