Abstract：
:Few techniques can identify interactions between proteins and individual water molecules when the protein is in solution. The present work has sought to bridge the gap between the molecular level studies and the search for a physical property of the solution (bathing the proteins) that would regulate the protein hydration level. The properties of the solution were varied by adding nondenaturing solutes and solvents to the protein solutions and then studying their effect on the intrinsic fluorescence of apomyoglobin. The resolution of the tryptophan emission into the two component spectra corresponding to tryptophans W7 (accessible to the solvent) and W14 (buried in the protein matrix) has allowed us to probe two specific parts of the protein. Whereas W14 is not affected when the medium is altered, the analysis of W7 fluorescence has shown that cosolvent diffusion plays a dominant role in the mobility of water molecules near the protein surface.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Twist C,Royer C,Alpert Bdoi
10.1021/bi012210+subject
Has Abstractpub_date
2002-08-13 00:00:00pages
10343-50issue
32eissn
0006-2960issn
1520-4995pii
bi012210+journal_volume
41pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:SufS is a type II cysteine desulfurase and acts as the initial step in the Suf Fe-S cluster assembly pathway. In Escherichia coli, this pathway is utilized under conditions of oxidative stress and is resistant to reactive oxygen species. Mechanistically, this means SufS must shift between protecting a covalent persulf...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b01122
更新日期：2019-02-12 00:00:00
abstract：:A stereochemical analysis has been carried out on two vinylpyruvate hydratases (VPH), which convert 2-hydroxy-2,4-pentadienoate to 2-keto-4S-hydroxypentanoate in meta-fission pathways. Bacterial strains with this pathway can use aromatic compounds as sole sources of energy and carbon. The analysis was carried out usin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00552
更新日期：2016-07-26 00:00:00
abstract：:The non-typeable Haemophilus influenzae strain DH1 was isolated from a 25 year old male patient with Fisher syndrome, a postinfectious autoimmune condition characterized by the presence of anti-GQ1b IgG antibodies that target and initiate damage to peripheral nerves. DH1 was found to display an alphaNeuAc(2-8)alphaNeu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi700685s
更新日期：2007-07-10 00:00:00
abstract：:The three-dimensional chalice-like crystal structure of initiation factor 2 IF2/eIF5B from Methanobacterium thermoautotrophicum represents a novel fold and domain architecture in which the N-terminal G domain and the C-terminal C domain are separated by an approximately 40 A alpha-helix. Homologous Thermus thermophilu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi702295g
更新日期：2008-04-29 00:00:00
abstract：:We report the use of electrospray ionization (ESI) mass spectrometry (MS) in conjunction with online rapid mixing to monitor the kinetics of acid-induced ferrihemoglobin denaturation. Under equilibrium conditions, the hemoglobin mass spectrum is dominated by the intact heterotetramer. Dimeric and monomeric species are...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi048501a
更新日期：2004-11-23 00:00:00
abstract：:The behavior of photosystem II (PSII) reactions was investigated under conditions of decreasing water content by the addition of increasing concentrations of ethylene glycol (EG). The photosynthetic activities were measured for PSII samples either directly in aqueous solutions of EG or in the standard buffer medium fo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961724j
更新日期：1997-01-07 00:00:00
abstract：:Rhodanese bound to bacterial ribosomes as peptidyl-tRNA can be folded into an enzymatically active conformation by generating C-terminal extensions of the wild-type enzyme. Rhodanese was synthesized by coupled transcription/translation in a cell-free Escherichia coli system from plasmids containing the coding sequence...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00044a003
更新日期：1995-11-07 00:00:00
abstract：:Neuropeptide Y (NPY) and the pancreatic polypeptide (PP) are members of the neuropeptide Y family of hormones. They bind to the Y receptors with very different affinities: Whereas PP is highly selective for the Y(4) receptor, NPY displays highest affinites for Y(1), Y(2), and Y(5) receptor subtypes. Introducing the NP...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0501232
更新日期：2005-06-28 00:00:00
abstract：:A preparation consisting of isolated dimeric peripheral antenna complexes from green plant photosystem I (light-harvesting complex I or LHCI) has been characterized by means of (polarized) steady-state absorption and fluorescence spectroscopy at low temperatures. We show that this preparation can be described reasonab...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0007369
更新日期：2000-07-25 00:00:00
abstract：:Interaction between aggregates of amyloid beta protein (Abeta) and membranes has been hypothesized by many to be a key event in the mechanism of neurotoxicity associated with Alzheimer's disease (AD). Proposed membrane-related mechanisms of neurotoxicity include ion channel formation, membrane disruption, changes in m...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9006397
更新日期：2009-09-22 00:00:00
abstract：:The presence of high titers of anti-cardiolipin antibodies (ACA's) of autoimmune origin, which are known to bind to plasma beta2-glycoprotein I (aka apolipoprotein H), correlates clinically with autoimmune recurrent thrombosis. Soluble beta2-glycoprotein I binds to solid-phase ACA (immobilized on a surface plasmon res...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9807207
更新日期：1998-11-17 00:00:00
abstract：:The aspartate receptors of Escherichia coli and Salmonella typhimurium which mediate chemotactic responsiveness to aspartate have 79% amino acid sequence identity but exhibited apparently quite different aspartate binding plots. The Scatchard plot of the Salmonella receptor was concave upward whereas the E. coli recep...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00169a002
更新日期：1994-01-25 00:00:00
abstract：:Dihydroorotates deuteriated at both C5 and C6 have been prepared and used to probe the mechanism of the bovine liver mitochondrial dihydroorotate dehydrogenase. Primary deuterium isotope effects on kcat are observed with both (6RS)-[5(S)-2H]- and (6RS)-[6-2H] dihydroorotates (3 and 6, respectively); these effects are ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00429a041
更新日期：1989-02-07 00:00:00
abstract：:The beta-glucosidase from Agrobacterium sp. catalyzes the hydrolysis of beta-glucosides via a covalent alpha-D-glucopyranosyl-enzyme intermediate involving Glu358. Hydrolysis of 2,4-dinitrophenyl beta-D-glucopyranoside by the low activity Glu358Asp mutant of Agrobacterium beta-glucosidase is accompanied by time-depend...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00044a033
更新日期：1995-11-07 00:00:00
abstract：:Amyloid diseases make up a set of fatal disorders in which proteins aggregate to form fibrils that deposit in tissues throughout the body. Amyloid-associated diseases are challenging to study because amyloid formation occurs on time scales that span several orders of magnitude and involve heterogeneous, interconvertin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.9b00769
更新日期：2019-11-05 00:00:00
abstract：:Thermobifida fusca Cel9A-90, an unusual family 9 enzyme, is a processive endoglucanase containing a catalytic domain closely linked to a family 3c cellulose binding domain (Cel9A-68) followed by a fibronectin III-like domain and a family 2 cellulose binding domain. To study its catalytic mechanism, 12 mutant genes wit...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049394n
更新日期：2004-08-03 00:00:00
abstract：:6-Hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK) catalyzes the pyrophosphoryl transfer from ATP to 6-hydroxymethyl-7,8-dihydropterin (HP), the first reaction in the folate biosynthetic pathway. Arginine residues 82 and 92, strictly conserved in 35 HPPK sequences, play dynamic roles in the catalytic cycle of ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0267994
更新日期：2003-02-18 00:00:00
abstract：:Human erythrocyte band 3 inhibits glycolytic enzymes, including aldolase, by binding these cytoplasmic enzymes at its N-terminus. Phosphorylation of Y8 disrupts inhibition, and there is evidence that in vivo glycolysis levels in erythrocytes are regulated in part by a phosphorylation/dephosphorylation signaling pathwa...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00051a005
更新日期：1995-12-26 00:00:00
abstract：:It is known that aconitase from mammalian mitochondria is only partially active as isolated but may be activated by incubation with iron, ascorbate, and a thiol, or with dithionite. It has been suggested that the added Fe in the activation mixture is essential for activation and that it is incorporated in the enzyme [...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00529a023
更新日期：1981-12-22 00:00:00
abstract：:Telomerase is a ribonucleoprotein that participates in the maintenance of telomere length. Its activity is up-regulated in many tumor types, suggesting that it may be a novel target for chemotherapy. The RNA component of telomerase contains an active site that plays at least two roles&sbd;binding telomere ends and tem...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970438k
更新日期：1997-09-30 00:00:00
abstract：:Membrane vesicles can be prepared from murine lymphoid cells by nitrogen cavitation and fractionated by sedimentation through nonlinear sucrose density gradients. Two subpopulations of membrane vesicles, PMI and PMII, can be distinguished on the basis of sedimentation rate. The subcellular distribution of adenylate an...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00652a026
更新日期：1976-04-06 00:00:00
abstract：:A mass transport theory of the gel chromatographic procedure of Hummel and Dreyer for measuring the binding of small ligand molecules to macromolecules has been formulated for ligand-mediated macromolecular association. It is concluded that the Hummel-Dreyer procedure is the method of choice for quantitating ligand bi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00666a011
更新日期：1976-10-19 00:00:00
abstract：:The structure/function properties of functional groups in the leadzyme have been studied by assaying the activity of analog ribozymes generated by the systematic substitution of modified nucleotides in the internal loop region of the ribozyme. Guanosine analogs introduced at positions 4 and 7 occupied by guanosine in ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi962219p
更新日期：1997-03-18 00:00:00
abstract：:A high molecular weight acyl coenzyme A (acyl-CoA) thioesterase, designated thioesterase II, has been purified 5300-fold from photoheterotrophically grown cells of Rhodopseudomonas sphaeroides. In contrast to R. sphaeroides acyl-CoA thioesterase I [Boyce, S.G., & Lueking, D.R. (1984) Biochemistry 23, 141-147], thioest...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00357a029
更新日期：1986-05-06 00:00:00
abstract：:d-2-Hydroxyglutarate dehydrogenase from Pseudomonas aeruginosa PAO1 (PaD2HGDH) catalyzes the oxidation of d-2-hydroxyglutarate to 2-ketoglutarate, which is a necessary step in the serine biosynthetic pathway. The dependence of P. aeruginosa on PaD2HGDH makes the enzyme a potential therapeutic target against P. aerugin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.0c00832
更新日期：2020-12-29 00:00:00
abstract：:The EphA2 receptor plays key roles in many physiological and pathological events, including cancer. The process of receptor endocytosis and the consequent degradation have attracted attention as possible means of overcoming the negative outcomes of EphA2 in cancer cells and decreasing tumor malignancy. A recent study ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300141h
更新日期：2012-03-13 00:00:00
abstract：:Solid-state NMR spectroscopy was employed to study the molecular dynamics of the colicin Ia channel domain in the soluble and membrane-bound states. In the soluble state, the protein executes small-amplitude librations (with root-mean-square angular fluctuations of 0-10 degrees ) in the backbone and larger-amplitude m...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0027231
更新日期：2001-06-26 00:00:00
abstract：:The stability of three forms of glucoamylase from Aspergillus niger has been investigated by differential scanning and isothermal titration calorimetry: Glucoamylase 1 (GA1), which consists of a catalytic domain and a starch-binding domain (SBD) connected by a heavily O-glycosylated linker region; glucoamylase 2 (GA2)...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi990185q
更新日期：1999-05-11 00:00:00
abstract：:The data and approach reported in paper I (Hanlon et al., 1975, preceding paper) have been used to calculate the fractional changes in secondary structure of calf thymus deoxyribonucleic acid which occur in aqueous solutions as a function of the concentration of NaCl, KCl, LiCl, CsCl, and NH4Cl. There is a continuous ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00679a018
更新日期：1975-04-22 00:00:00
abstract：:The present study was carried out to determine the energetics of Clostridium symbiosum pyruvate phosphate dikinase (PPDK) catalyzed interconversion of adenosine 5'-triphosphate (ATP), orthophosphate (Pi), and pyruvate (pyr) with adenosine 5'-monophosphate (AMP), inorganic pyrophosphate, and phosphoenolpyruvate (PEP) [...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00171a007
更新日期：1994-02-08 00:00:00
