Structural insight into how Streptomyces coelicolor maltosyl transferase GlgE binds α-maltose 1-phosphate and forms a maltosyl-enzyme intermediate.

abstract：:Tryptic digestion studies of the human erythrocyte glucose carrier have shown that a reactive and transport-sensitive exofacial sulfhydryl is located in the carboxy-terminal half of the molecule, corresponding to Cys347, Cys421, or Cys429. In the present studies, the erythrocyte glucose carrier labeled on the exofacia...

journal_title：Biochemistry

authors： May JM,Buchs A,Carter-Su C

更新日期：1990-11-13 00:00:00

abstract：:The thermodynamics and kinetics of magnesium binding to tRNA(Phe)(yeast) have been studied directly by 25Mg NMR. In 0.17 M Na+(aq), tRNA(Phe) exists in its native conformation and the number of strong binding sites (Ka greater than or equal to 10(4)) was estimated to be 3-4 by titration experiments, in agreement with ...

journal_title：Biochemistry

authors： Reid SS,Cowan JA

更新日期：1990-06-26 00:00:00

abstract：:P450cam from Pseudomonas putida is the best characterized member of the vast family of cytochrome P450s, and it has long been believed to have a more rigid and closed active site relative to other P450s. Here we report X-ray structures of P450cam crystallized in the absence of substrate and at high and low [K(+)]. The...

journal_title：Biochemistry

authors： Lee YT,Wilson RF,Rupniewski I,Goodin DB

更新日期：2010-04-27 00:00:00

abstract：:Microdomains known as "rafts" have been isolated from many cell types as detergent-resistant membranes (DRMs) and are enriched in sphingolipids and cholesterol. However, there has been considerable controversy over whether such domains are found in native membranes or are artificially generated by the purification pro...

journal_title：Biochemistry

authors： Luria A,Vegelyte-Avery V,Stith B,Tsvetkova NM,Wolkers WF,Crowe JH,Tablin F,Nuccitelli R

更新日期：2002-11-05 00:00:00

abstract：:NMR spectroscopic experiments were performed to study the structure of synthetic peptides identical to two extracellular regions of the human thrombin receptor. The smaller molecule, comprising 14 amino acids, was biologically active and was equivalent to the "tethered ligand" exposed after cleavage of the receptor by...

journal_title：Biochemistry

authors： Smith KJ,Trayer IP,Grand RJ

更新日期：1994-05-24 00:00:00

abstract：:D(--)-beta-Hydroxybutyrate dehydrogenase of beef heart mitochondria is inhibited by modifiers of disulfides, thiols, and vicinal dithiols [Phelps, D. C., & Hatefi, Y. (1981) Biochemistry 20 (preceding paper in this issue)]. The vicinal dithiol can be reversibly oxidized by diamide, resulting in activity inhibition, an...

journal_title：Biochemistry

authors： Phelps DC,Hatefi Y

更新日期：1981-02-03 00:00:00

abstract：:The Na+-translocating NADH:ubiquinone oxidoreductase (Na+-NQR) from Vibrio harveyi was purified and studied by EPR and visible spectroscopy. Two EPR signals in the NADH-reduced enzyme were detected: one, a radical signal, and the other a line around g = 1.94, which is typical for a [2Fe-2S] cluster. An E(m) of -267 mV...

journal_title：Biochemistry

authors： Bogachev AV,Bertsova YV,Barquera B,Verkhovsky MI

更新日期：2001-06-19 00:00:00

abstract：:Tafazzin is the mitochondrial enzyme that catalyzes transacylation between a phospholipid and a lysophospholipid in remodeling. Mutations in tafazzin cause Barth syndrome, a potentially life-threatening disease with the major symptom being cardiomyopathy. In the tafazzin-deficient heart, cardiolipin (CL) acyl chains b...

journal_title：Biochemistry

authors： Kimura T,Kimura AK,Ren M,Berno B,Xu Y,Schlame M,Epand RM

更新日期：2018-04-10 00:00:00

abstract：:It is now appreciated that the relationships of proteins, particularly enzymes, within a protein superfamily can be understood not only in terms of their sequence similarities and three-dimensional structures but also by chemical threads that relate their functional attributes. The mechanistic ties among superfamily m...

journal_title：Biochemistry

authors： Armstrong RN

更新日期：2000-11-14 00:00:00

abstract：:The involvement of Cl(-) and several other monovalent anions in photosynthetic oxygen evolution was studied using photosystem II membranes depleted of Cl(-) by dialysis. The results of these studies differ significantly from results obtained using other depletion methods. Binding studies with glycerol as a cryoprotect...

journal_title：Biochemistry

authors： Olesen K,Andréasson LE

更新日期：2003-02-25 00:00:00

abstract：:The proton nuclear Overhauser effects of bovine alpha-lactalbumin were studied at 200 MHz by irradiation of an upfield ring current shifted methylene at -2.45 ppm (assigned to Ile-95) and two aromatic protons, Tyr-103 (8.36 ppm) and Trp-60 (5.85 ppm). The experimental results were consistent with a putative three-dime...

journal_title：Biochemistry

authors： Koga K,Berliner LJ

更新日期：1985-12-03 00:00:00

abstract：:The unique structural properties of the ferritin protein cages have provided impetus to focus on the methodical study of these self-assembling nanosystems. Among these proteins, Escherichia coli bacterioferritin (EcBfr), although architecturally very similar to other members of the family, shows structural instability...

journal_title：Biochemistry

authors： Ardejani MS,Li NX,Orner BP

更新日期：2011-05-17 00:00:00

abstract：:The three-dimensional structure of toxic shock syndrome toxin 1 (TSST-1) from Staphylococcus aureus has been determined and refined to an R value of 0.226 for data between 8- and 2.5-A resolution. Overall, the structure of TSST-1 is similar to that of another superantigen, staphylococcal enterotoxin B (SEB). The key d...

journal_title：Biochemistry

authors： Prasad GS,Earhart CA,Murray DL,Novick RP,Schlievert PM,Ohlendorf DH

更新日期：1993-12-21 00:00:00

abstract：:The Wilson disease copper-transporting ATPase plays a critical role in the intracellular trafficking of copper. Mutations in this protein lead to the accumulation of a toxic level of copper in the liver, kidney, and brain followed by extensive tissue damage and death. The ATPase has a novel amino-terminal domain ( app...

journal_title：Biochemistry

authors： DiDonato M,Hsu HF,Narindrasorasak S,Que L Jr,Sarkar B

更新日期：2000-02-22 00:00:00

abstract：:To better understand how amino acid sequences specify unique tertiary folds, we have used random mutagenesis and phage display selection to evolve proteins with a high degree of sequence identity but different tertiary structures (homologous heteromorphs). The starting proteins in this evolutionary process were the Ig...

journal_title：Biochemistry

authors： Alexander PA,Rozak DA,Orban J,Bryan PN

更新日期：2005-11-01 00:00:00

abstract：:Bovine seminal ribonuclease (BS-RNase), the only dimeric protein among the pancreatic-like ribonucleases, is endowed with special structural features and with biological functions beyond enzymatic activity. In solution, the protein exists as an equilibrium mixture of two forms, with or without exchange (or swapping) o...

journal_title：Biochemistry

authors： Avitabile F,Alfano C,Spadaccini R,Crescenzi O,D'Ursi AM,D'Alessio G,Tancredi T,Picone D

更新日期：2003-07-29 00:00:00

abstract：:The interaction of poly[(G-C)] and poly[d(G-m5C)] with the antitumor antibiotic elsamicin A, which binds to alternating guanine + cytosine tracts in DNA, has been studied under the B and Z conformations. Both the rate and the extent of the B-to-Z transition are diminished by the antibiotic, as inferred by spectroscopi...

journal_title：Biochemistry

authors： Jiménez-García E,Portugal J

更新日期：1992-11-24 00:00:00

abstract：:The ubiquitous calcium-binding protein calmodulin (CaM) regulates a wide variety of cellular events by binding to and activating many distinct target enzymes. The CaM-binding domains of most of these enzymes are contained in a contiguous stretch of amino acids with a length of approximately 20 residues. In this work, ...

journal_title：Biochemistry

authors： Zhang M,Fabian H,Mantsch HH,Vogel HJ

更新日期：1994-09-13 00:00:00

abstract：:The peptide cyclo(hex-Amb(1)-Cys(2))-Thr(3)-Val(4)-Thr(5)-Nph(6)-NH2 was previously shown to be a slow, tight-binding inhibitor (Ki = 37 nM) of the yeast oligosaccharyl transferase (OT) [Hendrickson et al. (1996) J. Am. Chem. Soc. 118, 7636-7637]. This enzyme catalyzes the transfer of a carbohydrate moiety to an aspar...

journal_title：Biochemistry

authors： Kellenberger C,Hendrickson TL,Imperiali B

更新日期：1997-10-14 00:00:00

abstract：:Azotobacter vinelandii bacterioferritin (AvBF) containing 800-1500 Co or Mn atoms as Co(III) and Mn(III) oxyhydroxide cores (Co-AvBF, Mn-AvBF) was synthesized by the same procedure used previously for horse spleen ferritin (HoSF). The kinetics of reduction of Co-AvBF and Mn-AvBF by ascorbic acid are first-order in eac...

journal_title：Biochemistry

authors： Zhang B,Harb JN,Davis RC,Choi S,Kim JW,Miller T,Chu SH,Watt GD

更新日期：2006-05-09 00:00:00

abstract：:The subgel phases of a homologous series of saturated straight-chain diacylphosphatidylcholines with hydrocarbon chains consisting of 10-18 carbon atoms were studied by Fourier-transform infrared spectroscopy. All of these lipids initially form a subgel phase which is spectroscopically similar to that obtained when fu...

journal_title：Biochemistry

authors： Lewis RN,McElhaney RN

更新日期：1990-08-28 00:00:00

abstract：:alpha-1,3-Fucosyltransferase V (FucT V) catalyzes the transfer of 1-fucose from the donor sugar guanosine 5'-diphospho-beta-1-fucose (GDP-Fuc) to an acceptor sugar. A secondary isotope effect on the fucosyltransfer reaction with guanosine 5'-diphospho-[1-2H]-beta-1-fucose (GDP-[1-2H]-Fuc) as the substrate was observed...

journal_title：Biochemistry

authors： Murray BW,Wittmann V,Burkart MD,Hung SC,Wong CH

更新日期：1997-01-28 00:00:00

abstract：:Extensive molecular modeling with molecular dynamics simulations and van der Waals energy analyses were used to elucidate the striking finding that a mutagenic benzo[a]pyrene-derived DNA lesion, the base-displaced intercalated 10R-(+)-cis-anti-B[a]P-N(2)-dG (G*), manifests large differences in nucleotide excision repa...

journal_title：Biochemistry

authors： Mu H,Kropachev K,Chen Y,Zhang H,Cai Y,Geacintov NE,Broyde S

更新日期：2013-08-20 00:00:00

abstract：:The folding behavior of bovine growth hormone (bGH) is examined by chemical and pH denaturation using several spectroscopic probes of protein secondary and tertiary structure. Partially denaturing concentrations of urea eliminate the native-state quenching of intrinsic tryptophan fluorescence, from the single protein ...

journal_title：Biochemistry

authors： Holzman TF,Dougherty JJ Jr,Brems DN,MacKenzie NE

更新日期：1990-02-06 00:00:00

abstract：:Steady-state and transient-state analysis of Coprinus cinereus peroxidase, CIP (identical to Arthromyces ramosus peroxidase), was used to characterize the kinetics of the three fundamental steps in heme peroxidase catalysis: compound I (cpd I) formation, cpd I reduction, and compound II (cpd II) reduction. The rate co...

journal_title：Biochemistry

authors： Abelskov AK,Smith AT,Rasmussen CB,Dunford HB,Welinder KG

更新日期：1997-08-05 00:00:00

abstract：:Hybridization to RNA is important for many applications, including antisense therapeutics, RNA interference, and microarray screening. Similar thermodynamic stabilities of A-U and G-U base pairs result in difficulties in selective binding to RNA. Moreover, A-U pairs are weaker than G-C pairs so that binding is sometim...

journal_title：Biochemistry

authors： Carlucci M,Kierzek E,Olejnik A,Turner DH,Kierzek R

更新日期：2009-11-24 00:00:00

abstract：:Initial rate kinetic studies and reduced coenzyme binding studies with bovine glutamate dehydrogenase have shown that an enzyme-NAD(P)H-glutamate abortive complex is a major participant in the overall enzyme-catalyzed reaction. The allosteric regulator ADP is shown to activate the enzyme by destabilizing this abortive...

journal_title：Biochemistry

authors： George A,Bell JE

更新日期：1980-12-23 00:00:00

abstract：:The mature form of subtilisin is an unusual example of a monomeric protein with a high kinetic barrier to folding and unfolding. Using site-directed mutagenesis of subtilisin BPN', we are attempting to determine the physical and energetic nature of the kinetic barrier. The high-affinity calcium-binding site A has been...

journal_title：Biochemistry

authors： Strausberg S,Alexander P,Wang L,Gallagher T,Gilliland G,Bryan P

更新日期：1993-10-05 00:00:00

abstract：:Assembly of the type I procollagen molecule begins with interactions among the C-pro alpha1(I) and C-pro alpha2(I) domains. The C-propeptide domains themselves have subdomains of distinct structures. The important questions are where chain association begins and the basis of the chain selectivity which leads to the pr...

journal_title：Biochemistry

authors： Malone JP,Alvares K,Veis A

更新日期：2005-11-22 00:00:00

abstract：:The early redox events involved in iron reduction and mobilization in mammalian ferritin have been investigated by several techniques. Sedimentation velocity measurements of ferritin samples with altered core sizes, prepared by partial reduction and Fe2+ chelation, suggest two different events occur during iron loss f...

journal_title：Biochemistry

authors： Jacobs DL,Watt GD,Frankel RB,Papaefthymiou GC

更新日期：1989-02-21 00:00:00