Abstract:
:The subgel phases of a homologous series of saturated straight-chain diacylphosphatidylcholines with hydrocarbon chains consisting of 10-18 carbon atoms were studied by Fourier-transform infrared spectroscopy. All of these lipids initially form a subgel phase which is spectroscopically similar to that obtained when fully hydrated multilamellar dispersions of dipalmitoylphosphatidylcholine are incubated at 0-4 degrees C for 2-4 days. However, further low-temperature incubation of those phosphatidylcholines with acyl chains of 16 or fewer carbon atoms results in the sequential formation of 1 or more additional, spectroscopically distinct subgel phases, with the number of such phases increasing as hydrocarbon chain length decreases. Our data indicate that the formation of all of these subgel phases involves both reorientation of the acyl chains and major changes in hydration and/or hydrogen-bonding interactions at the polar/apolar interfacial region of the lipid bilayer. We suggest that the driving force behind the formation of these Lc phases is the formation of an extended hydrogen-bonding network in the interfacial region of the bilayer and that the optimization of this network probably requires some distortion of the optimal packing of the acyl chains. As a result, an increase in acyl chain length makes the formation of these Lc phases less favorable and eventually prevents optimization of the hydrogen-bonding network at the bilayer polar/apolar interface.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Lewis RN,McElhaney RNdoi
10.1021/bi00486a024subject
Has Abstractpub_date
1990-08-28 00:00:00pages
7946-53issue
34eissn
0006-2960issn
1520-4995journal_volume
29pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The explosion of protein sequence information requires that current strategies for function assignment evolve to complement experimental approaches with computationally based function prediction. This necessitates the development of strategies based on the identification of sequence markers in the form of specificity ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi201181q
更新日期:2011-10-18 00:00:00
abstract::Recent models for water oxidation in photosystem II postulate that the tyrosine Y(Z) radical, Y(Z)(*), abstracts both an electron and a proton from the Mn cluster during one or more steps in the catalytic cycle. This coupling of proton- and electron-transfer events is postulated to provide the necessary driving force ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi992749w
更新日期:2000-05-30 00:00:00
abstract::The mechanism by which the contractile machinery of muscle is assembled and maintained is not well-understood. Members of the cysteine-rich protein (CRP) family have been implicated in these processes. Three vertebrate CRPs (CRP1-3) that exhibit developmentally regulated muscle-specific expression have been identified...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi982036y
更新日期:1999-05-04 00:00:00
abstract::Dihydroorotate dehydrogenases (DHODs) oxidize dihydroorotate (DHO) to orotate using the FMN prosthetic group to abstract a hydride equivalent from C6 and a protein residue (Ser for Class 2 DHODs) to deprotonate C5. The fundamental question of whether the scission of the two DHO C-H bonds is concerted or stepwise was a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi060919g
更新日期:2006-12-19 00:00:00
abstract::Human plasma phospholipid transfer protein (PLTP) exchanges phospholipids between lipoproteins and remodels high-density lipoproteins (HDLs). We determined phospholipid transfer activity and HDL binding ability in wild-type PLTP and in 16 PLTP variants created by replacing 12 charged amino acids by site-directed mutag...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi027006g
更新日期:2003-04-22 00:00:00
abstract::Antithrombin III (ATIII) is a plasma-borne serine protease inhibitor that apparently forms covalent complexes with thrombin. The interaction between ATIII and thrombin is enhanced several thousandfold by the glycosaminoglycan, heparin. We have previously proposed that the heparin binding site of ATIII resides within a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00490a010
更新日期:1990-09-25 00:00:00
abstract::Nuclear DNA of the marine chromophytic alga Olisthodiscus luteus was analyzed in this study. Reassociation kinetics analysis has shown that 440-nucleotide DNA fragments from the genome of this alga contain 4% foldback, 58% repetitive, and 34% single-copy sequences. Precise analysis using isolated single-copy DNA revea...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00527a023
更新日期:1981-11-24 00:00:00
abstract::The biochemical characteristics of the formation and disappearance of intercalator-induced DNA double-strand breaks (DSB) were studied in nuclei from mouse leukemia L1210 cells by using filter elution methodology [Bradley, M. O., & Kohn, K.W. (1979) Nucleic Acids Res. 7, 793-804]. The three intercalators used were 4'-...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00309a013
更新日期:1984-07-03 00:00:00
abstract::RNA pur*pur-pyr and pyr*pur-pyr (pur = purine, pyr = pyrimidine) triple helices consisting of a Watson-Crick base-paired 28mer hairpin duplex and a Hoogsteen base-paired purine or pyrimidine 12mer are targeted with photoactivated cleavage by the metal complex Rh(phen)2phi3+ (phen = phenanthroline, phi = 9, 10-phenanth...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi980509v
更新日期:1998-06-23 00:00:00
abstract::The recently isolated Escherichia coli murB gene (Pucci et al., 1992) has been cloned into an expression vector and the encoded UDP-N-acetylenolpyruvylglucosamine reductase (EC 1.1.1.158) was overproduced to about 10% of soluble cell protein. The encoded 38-kDa protein has been purified to near homogeneity. It was fou...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00059a019
更新日期:1993-03-02 00:00:00
abstract::The behavior of fluorescent and spin-label probes is examined in several fluid and gel phospholipid phases, with particular focus on the Ca2+-induced gel phase in phosphatidylserine (PS). These probes have behavior characteristic of the type of probe and of the type of lipid environment. Anthroyloxy- and doxyl-labeled...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00380a039
更新日期:1987-03-24 00:00:00
abstract::Dopamine (DA) is an unstable neurotransmitter that readily oxidizes to the DA quinone and forms reactive oxygen species, such as superoxide and hydrogen peroxide. The oxidized dopamine also forms thiol conjugates with sulfhydryl groups on cysteine, glutathione, and proteins. In the present study, we determined the red...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0614434
更新日期:2006-12-19 00:00:00
abstract::The technique of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) has been applied to a mesophilic (E. coli) dihydrofolate reductase under conditions that allow direct comparison to a thermophilic (B. stearothermophilus) ortholog, Ec-DHFR and Bs-DHFR, respectively. The analysis of hydrogen-deuterium e...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200640s
更新日期:2011-09-27 00:00:00
abstract::The seven serotypes (A-G) of botulinum neurotoxins (BoNTs) function through their proteolytic cleavage of one of three proteins (SNAP-25, Syntaxin, and VAMP) that form the SNARE complex required for synaptic vesicle fusion. The different BoNTs have very specific protease recognition requirements, between 15 and 50 ami...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi052518r
更新日期:2006-03-14 00:00:00
abstract::Protein misfolding and aggregation is a ubiquitous phenomenon associated with a wide range of diseases. The synuclein family comprises three small naturally unfolded proteins implicated in neurodegenerative diseases and some forms of cancer. α-Synuclein is a soluble protein that forms toxic inclusions associated with ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300478w
更新日期:2012-06-12 00:00:00
abstract::Eukaryotic assimilatory nitrate reductase (NR) is a dimeric multidomain molybdo-heme-flavo protein that catalyzes the first and rate-limiting step in the nitrate assimilation of plants, algae, and fungi. Nitrate reduction takes place at the N-terminal molybdenum cofactor-containing domain. Reducing equivalents are der...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi1003487
更新日期:2010-09-21 00:00:00
abstract::A putative catalytic triad consisting of tyrosine, serine, and lysine residues was identified in the ketoreductase (KR) domains of modular polyketide synthases (PKSs) based on homology modeling to the short chain dehydrogenase/reductase (SDR) superfamily of enzymes. This was tested by constructing point mutations for ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0268706
更新日期:2003-01-14 00:00:00
abstract::Porcine parathyroid gland slices were incubated with 3H-labeled amino acids in order to label tissue proteins. After incubation a crude hormonal extract was prepared and analyzed by chromatography on carboxymethylcellulose. Among the three radioactive peaks which were detected in the eluate, two were identified as par...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00687a018
更新日期:1975-08-12 00:00:00
abstract::Here we report that the Methanocaldococcus jannaschii enzyme derived from the MJ0309 gene is an Fe(II) dependent agmatinase (SpeB). This is the first report of an iron-dependent agmatinase. We demonstrate that aerobically isolated recombinant enzyme contains two disulfide bonds and only a trace amount of any metal and...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300039f
更新日期:2012-04-10 00:00:00
abstract::The prokaryotic DNA protection during starvation (Dps) proteins typically protect macromolecules against damaging agents via physical association with DNA and by oxidizing and sequestering iron. However, Deinococcus radiodurans Dps-1, which binds DNA with high affinity, fails to protect DNA against hydroxyl radicals d...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300703x
更新日期:2012-08-21 00:00:00
abstract::The HpxO enzyme from Klebsiella pneumoniae was recently proposed, on the basis of genetic studies, to catalyze the hydroxylation of uric acid to 5-hydroxyisourate as part of the purine catabolic pathway. Its primary sequence suggests that the HpxO catalytic activity depends on a flavin cofactor (FAD), contrasting with...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi900160b
更新日期:2009-04-14 00:00:00
abstract::A high-resolution solution conformation of a novel conotoxin, [Pro 7,13] alpha A-conotoxin PIVA, GCCGSYPNAACHPCSCKDROSYCGQ-NH2, has been determined by two-dimensional 1H NMR methods and distance geometry calculations. The total of 324 NOE-derived interproton distance restraints including 33 long-range NOE restraints a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi962301k
更新日期:1997-02-18 00:00:00
abstract::We have investigated the role of the labile terminal domains of the core histones on the stability of the subunits of the protein core of the nucleosome by studying the thermodynamic behavior of the products of limited trypsin digestion of these subunits. The thermal stabilities of the truncated H2A-H2B dimer and the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0110140
更新日期:2001-10-30 00:00:00
abstract::Blood coagulation is initiated by Ca(2+)-dependent binding of coagulation factor VIIa (FVIIa) to its cofactor, tissue factor (TF). The TF:FVIIa complex activates factors IX and X, ultimately leading to the formation of thrombin and the coagulation of blood. FVII consists of an N-terminal gamma-carboxyglutamic-acid-con...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi980522f
更新日期:1998-07-28 00:00:00
abstract::The cystic fibrosis transmembrane conductance regulator forms an anion-selective channel. We previously showed that charge selectivity, the ability to discriminate between anions and cations, occurs near the cytoplasmic end of the channel. The molecular determinants of charge selectivity, however, are unknown. We inve...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990155n
更新日期:1999-04-27 00:00:00
abstract::Redox transitions in the Rhodobacter capsulatus cytochrome bc(1) complex were investigated by perfusion-induced attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy combined with synchronous visible spectroscopy, in both the wild type and a cytochrome c(1) point mutant, M183K, in which the mi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049211x
更新日期:2004-07-27 00:00:00
abstract::The binding of Ca2+ and the resulting change in catalytic specificity that allows phosphorylation of the calcium ATPase of sarcoplasmic reticulum by ATP were examined by measuring the amount of phosphoenzyme formation from [32P]ATP, or 45Ca incorporation into vesicles, after the simultaneous addition of ATP and EGTA a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00423a018
更新日期:1988-11-15 00:00:00
abstract::By employing a general biosynthetic method for the elaboration of proteins containing unnatural amino acid analogues, we incorporated (aminooxy)acetic acid into positions 10 and 27 of Escherichia coli dihydrofolate reductase. Introduction of the modified amino acid into DHFR was accomplished in an in vitro protein bio...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi020352d
更新日期:2002-09-24 00:00:00
abstract::Only beta-beta cross-links form when the alpha(3)(betaE(395)C)(3)gammaK(36)C (MF(1) residue numbers) double mutant subcomplex of TF(1), the F(1)-ATPase from the thermophilic Bacillus PS3, is slowly inactivated with CuCl(2) in the presence or absence of MgATP. The same slow rate of inactivation and extent of beta-beta ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi036058i
更新日期:2004-03-09 00:00:00
abstract::Living organisms have evolved a vast array of catalytic functions that make them ideally suited for the production of medicinally and industrially relevant small-molecule targets. Indeed, native metabolic pathways in microbial hosts have long been exploited and optimized for the scalable production of both fine and co...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/bi200416g
更新日期:2011-06-21 00:00:00