Human transforming growth factor type beta 2: production by a prostatic adenocarcinoma cell line, purification, and initial characterization.

abstract：:Four polypeptide chains composing acetylcholine receptors from the electric organ of Torpedo californica were purified by preparative electrophoresis in sodium dodecyl sulfate. Their apparent mole ratio alpha/beta/gamma/delta is 2:1:1:1. These chains are not readily distinguished by amino acid or carbohydrate composit...

journal_title：Biochemistry

authors： Lindstrom J,Merlie J,Yogeeswaran G

更新日期：1979-10-16 00:00:00

abstract：:Protein kinases are key regulators of diverse signaling networks critical for growth and development. Protein kinase A (PKA) is an important kinase prototype that phosphorylates protein targets at Ser and Thr residues by converting ATP to ADP. Mg(2+) ions play a crucial role in regulating phosphoryl transfer and can l...

journal_title：Biochemistry

authors： Khavrutskii IV,Grant B,Taylor SS,McCammon JA

更新日期：2009-12-08 00:00:00

abstract：:Thiolases are CoA-dependent enzymes which catalyze the formation of a carbon-carbon bond in a Claisen condensation step and its reverse reaction via a thiolytic degradation mechanism. Mitochondrial acetoacetyl-coenzyme A (CoA) thiolase (T2) is important in the pathways for the synthesis and degradation of ketone bodie...

journal_title：Biochemistry

authors： Haapalainen AM,Meriläinen G,Pirilä PL,Kondo N,Fukao T,Wierenga RK

更新日期：2007-04-10 00:00:00

abstract：:Thermodynamic and structural evidence indicates that the DNA binding domains of lac repressor (lacI) exhibit significant conformational adaptability in operator binding, and that the marginally stable helix-turn-helix (HTH) recognition element is greatly stabilized by operator binding. Here we use circular dichroism a...

journal_title：Biochemistry

authors： Felitsky DJ,Record MT Jr

更新日期：2003-02-25 00:00:00

abstract：:Spectroscopic methods are used to probe the interactions of several anilinoacridine analogues with calf thymus DNA over a wide range of temperatures and sodium chloride concentrations. The structurally similar compounds m-AMSA, AMSA (both active as antitumor agents), and o-AMSA (inactive as an antitumor agent) have be...

journal_title：Biochemistry

authors： Wadkins RM,Graves DE

更新日期：1991-04-30 00:00:00

abstract：:Retinoid X receptors (RXRs) are nuclear receptors that can activate transcription as homodimers or as obligate heterodimeric partners of other nuclear receptors. While the crystal structures of the RXR ligand-binding domains (LBD) have been previously determined, the dynamics of activation is less well characterized a...

journal_title：Biochemistry

authors： Lu J,Cistola DP,Li E

更新日期：2006-02-14 00:00:00

abstract：:We have determined the nucleotide sequence and the derived amino acid sequence of the phosphoprotein-encoding region of the chicken vitellogenin III gene. The sequence of this minor vitellogenin could be aligned with exon 22 up to exon 27 of the previously sequenced major vitellogenin II gene (van het Schip et al., 19...

journal_title：Biochemistry

authors： Byrne BM,de Jong H,Fouchier RA,Williams DL,Gruber M,Ab G

更新日期：1989-03-21 00:00:00

abstract：:Intensive study has been devoted to understanding the kinetic and structural bases underlying the exceptionally high fidelity (low error frequencies) of the typical DNA polymerase. Commonly proposed explanations have included (i) the concept of fidelity check points, in which the correctness of a nascent base pair mat...

journal_title：Biochemistry

authors： Showalter AK,Tsai MD

更新日期：2002-08-27 00:00:00

abstract：:A recombinant system was developed for generation of steroid-receptor complexes in vitro. The DNA- and steroid-binding domains of the rat mineralocorticoid receptor were expressed in Escherichia coli as a fusion protein with glutathione S-transferase. The identity of the expressed recombinant protein was confirmed by ...

journal_title：Biochemistry

authors： Caamaño CA,Morano MI,Patel PD,Watson SJ,Akil H

更新日期：1993-08-24 00:00:00

abstract：:Chicken nucleoside triphosphate diphosphohydrolase 8 (NTPDase8) is a cell surface ectonucleotidase with a large extracellular domain (ECD) containing the active site and is anchored to the membrane by two transmembrane domains (TMDs) at the N- and C-termini. Unlike other cell surface NTPDases that have been characteri...

journal_title：Biochemistry

authors： Li CS,Lee Y,Knowles AF

更新日期：2010-01-12 00:00:00

abstract：:Escherichia coli class Ib ribonucleotide reductase (RNR) converts nucleoside 5'-diphosphates to deoxynucleoside 5'-diphosphates in iron-limited and oxidative stress conditions. We have recently demonstrated in vitro that this RNR is active with both diferric-tyrosyl radical (Fe(III)(2)-Y(•)) and dimanganese(III)-Y(•) ...

journal_title：Biochemistry

authors： Cotruvo JA,Stubbe J

更新日期：2011-03-15 00:00:00

abstract：:The evolutionarily conserved DRY motif at the end of the third helix of rhodopsin-like, class-A G protein-coupled receptors (GPCRs) is a major regulator of receptor stability, signaling activity, and β-arrestin-mediated internalization. Substitution of the DRY arginine with histidine in the human vasopressin receptor ...

journal_title：Biochemistry

authors： Burczyk M,Burkhalter MD,Blätte T,Matysik S,Caron MG,Barak LS,Philipp M

更新日期：2015-01-27 00:00:00

abstract：:The 70-kDa heat-shock protein (Hsp70) has been cloned and sequenced from bovine cardiac muscle. On the basis of sequence features, the gene corresponds to the cytoplasmic form of Hsp70. This cardiac Hsp70 cDNA clone has an open reading frame of 1926 bp coding for 641 amino acids and a predicted molecular mass of 70.25...

journal_title：Biochemistry

authors： Lakshmikuttyamma A,Selvakumar P,Anderson DH,Datla RS,Sharma RK

更新日期：2004-10-26 00:00:00

abstract：:The carbon-13 nuclear magnetic resonance (13C NMR) spectra of luteinizing hormone-releasing hormone (LH-RH) and lower homologous peptides have been assigned in aqueous solutions at various pH values. 13C spin-lattice relaxation times (T1) have been measured for all proton-bearing carbons at 25.2 and 67.9 MHz. From the...

journal_title：Biochemistry

authors： Deslauriers R,Levy GC,McGregor WH,Sarantakis K,Smith IC

更新日期：1975-09-23 00:00:00

abstract：:The gene 5 protein (g5p) encoded by filamentous Ff phages is an ssDNA-binding protein, which binds to and sequesters the nascent ssDNA phage genome in the process of phage morphogenesis. The g5p also binds with high affinity to DNA and RNA sequences that form G-quadruplex structures. However, sequences that would form...

journal_title：Biochemistry

authors： Wen JD,Gray DM

更新日期：2004-03-09 00:00:00

abstract：:Light chain amyloidoses arise from the overproduction and abnormal deposition of the immunoglobulin light chain in various organs. LEN is the variable domain of an immunoglobulin light chain originally isolated from the urine of a patient suffering from multiple myeloma, with no sign of renal dysfunction or amyloidosi...

journal_title：Biochemistry

authors： Hu D,Qin Z,Xue B,Fink AL,Uversky VN

更新日期：2008-08-19 00:00:00

abstract：:Membrane vesicles from Mycobacterium phlei contain carrier proteins for proline, glutamine, and glutamic acid. The transport of proline is Na+ dependent and required substrate oxidation. A proline carrier protein was solubilized from the membrane vesicles by treatment with cholate and Triton X-100. Electron microscopi...

journal_title：Biochemistry

authors： Lee SH,Cohen NS,Jacobs AJ,Brodie AF

更新日期：1979-05-29 00:00:00

abstract：:The X-ray crystal structure of the complex of the B-DNA dodecamer CGCGAATTCGCG with the antitumor drug netropsin has been reexamined to locate the drug accurately for computer-based drug design. The optimum solution is with the drug centered in the AATT region of the minor groove, making three good bifurcated hydrogen...

journal_title：Biochemistry

authors： Goodsell DS,Kopka ML,Dickerson RE

更新日期：1995-04-18 00:00:00

abstract：:Purine nucleoside phosphorylase (PNP) catalyzes the reversible phosphorolysis of purine ribonucleosides and 2'-deoxyribonucleosides, yielding the purine base and (2'-deoxy)ribose 1-phosphate as products. While this enzyme has been extensively studied, several questions with respect to the catalytic mechanism have rema...

journal_title：Biochemistry

authors： Isaksen GV,Hopmann KH,Åqvist J,Brandsdal BO

更新日期：2016-04-12 00:00:00

abstract：:Cytochrome b559 (cyt b559) is an intrinsic and essential component of the photosystem II (PSII) protein complex, but its function, stoichiometry, and electron-transfer kinetics in the physiological system are not well-defined. In this study, we have used flash-detection optical spectroscopy to measure the kinetics and...

journal_title：Biochemistry

authors： Buser CA,Diner BA,Brudvig GW

更新日期：1992-11-24 00:00:00

abstract：:An antibody population that reacted with the high-affinity lysine binding site of human plasminogen was elicited by immunizing rabbits with an elastase degradation product containing kringles 1-3 (EDP I). This antibody was immunopurified by affinity chromatography on plasminogen-Sepharose and elution with 0.2 M 6-amin...

journal_title：Biochemistry

authors： Miles LA,Plow EF

更新日期：1986-11-04 00:00:00

abstract：:Electrogenic events associated with the activity of the melibiose permease (MelB), a transporter from Escherichia coli, were investigated. Proteoliposomes containing purified MelB were adsorbed to a solid supported lipid membrane, activated by a substrate concentration jump, and transient currents were measured. When ...

journal_title：Biochemistry

authors： Meyer-Lipp K,Ganea C,Pourcher T,Leblanc G,Fendler K

更新日期：2004-10-05 00:00:00

abstract：:Molecular dynamics (MD) simulations of Thermus thermophilus chorismate mutase substrate complex (TtCM x S) have been carried out at 298 K, 333 K, and the temperature of optimum activity: 343 K. The enzyme exists as trimeric subunits with active sites shared between two neighboring subunits. Two features distinguish in...

journal_title：Biochemistry

authors： Zhang X,Bruice TC

更新日期：2006-07-18 00:00:00

abstract：:The product of transesterification of phospholipid acyl chains and unesterified cholesterol (UC) by the enzyme lecithin:cholesterol acyltransferase (LCAT) is cholesteryl ester (CE). Activation of LCAT by apolipoprotein (apo) A-I on nascent (discoidal) high-density lipoproteins (HDL) is essential for formation of matur...

journal_title：Biochemistry

authors： Jones MK,Catte A,Li L,Segrest JP

更新日期：2009-12-01 00:00:00

abstract：:A cDNA coding for a P450 expressed in human lung was isolated from a lambda gt11 library constructed from human lung mRNA using a cDNA probe to rat P450 IVA1. The cDNA-deduced amino acid sequence of this P450, designated IVB1, consisted of 511 amino acids and had a calculated molecular weight of 59,558. The IVB1 amino...

journal_title：Biochemistry

authors： Nhamburo PT,Gonzalez FJ,McBride OW,Gelboin HV,Kimura S

更新日期：1989-10-03 00:00:00

abstract：:Exoenzyme S (ExoS) is a bifunctional virulence factor directly translocated into eukaryotic cells by the type III secretory process of Pseudomonas aeruginosa. Bacterial translocation of ExoS into epithelial cells is associated with diverse effects on cell function, including inhibition of growth, alterations in cell m...

journal_title：Biochemistry

authors： Fraylick JE,Riese MJ,Vincent TS,Barbieri JT,Olson JC

更新日期：2002-07-30 00:00:00

abstract：:Beta-lactamases are the most widespread resistance mechanism to beta-lactam antibiotics, such as the penicillins and cephalosporins. Transition-state analogues that bind to the enzymes with nanomolar affinities have been introduced in an effort to reverse the resistance conferred by these enzymes. To understand the or...

journal_title：Biochemistry

authors： Roth TA,Minasov G,Morandi S,Prati F,Shoichet BK

更新日期：2003-12-16 00:00:00

abstract：:NMR spectroscopy is particularly adept at site-specifically monitoring dynamic processes in proteins, such as protein folding, domain movements, ligand binding, and side-chain rotations. By coupling the favorable spectroscopic properties of highly dynamic side-chain methyl groups with transverse-relaxation-optimized s...

journal_title：Biochemistry

authors： Boswell ZK,Latham MP

更新日期：2019-01-22 00:00:00

abstract：:We present a new design of mixed-backbone antisense oligonucleotides (ASOs) containing both DNA and peptide nucleic acid (PNA). Previous generations of PNA-DNA chimeras showed low binding affinity, reducing their potential as therapeutics. The addition of a 5'-wing of locked nucleic acid as well as the combination of ...

journal_title：Biochemistry

authors： Debacker AJ,Sharma VK,Meda Krishnamurthy P,O'Reilly D,Greenhill R,Watts JK

更新日期：2019-02-12 00:00:00

abstract：:FLI1 (Friend leukemia integration 1) is a metazoan transcription factor that is upregulated in a number of cancers. In addition, rearrangements of the fli1 gene cause sarcomas, leukemias, and lymphomas. These rearrangements encode oncogenic transcription factors, in which the DNA binding domain (DBD or ETS domain) of ...

journal_title：Biochemistry

authors： Hou C,Tsodikov OV

更新日期：2015-12-22 00:00:00