Abstract:
:In contrast to FK506 binding proteins and cyclophilins, the parvulin family of peptidyl-prolyl cis/trans isomerases (PPIases; E.C. 5.2.1.8) cannot be inhibited by either FK506 or cyclosporin A. We have found that juglone, 5-hydroxy-1,4-naphthoquinone, irreversibly inhibits the enzymatic activity of several parvulins, like the E. coli parvulin, the yeast Ess1/Ptf1, and human Pin1, in a specific manner, thus allowing selective inactivation of these enzymes in the presence of other PPIases. The mode of action was studied by analyzing the inactivation kinetics and the nature of products of the reaction of E. coli parvulin and its Cys69Ala variant with juglone. For all parvulins investigated, complete inactivation was obtained by a slow process that is characterized by pseudo-first-order rate constants in the range of 5.3 x 10(-)4 to 4. 5 x 10(-)3 s-1. The inactivated parvulin contains two juglone molecules that are covalently bound to the side chains of Cys41 and Cys69 because of a Michael addition of the thiol groups to juglone. Redox reactions did not contribute to the inactivation process. Because thiol group modification was shown to proceed 5-fold faster than the rate of enzyme inactivation, it was considered as a necessary but not sufficient condition for inactivation. When measured by far-UV circular dichroism (CD), the rate of structural alterations following thiol group modification parallels exactly the rate of inactivation. Thus, partial unfolding of the active site of the parvulins was thought to be the cause of the deterioration of PPIase activity.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Hennig L,Christner C,Kipping M,Schelbert B,Rücknagel KP,Grabley S,Küllertz G,Fischer Gdoi
10.1021/bi973162psubject
Has Abstractpub_date
1998-04-28 00:00:00pages
5953-60issue
17eissn
0006-2960issn
1520-4995pii
bi973162pjournal_volume
37pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The substrate-bound form of the enzyme heme oxygenase (HO), which catalyzed the stereospecific alpha-meso bridge cleavage of hemin to yield biliverdin IX alpha, has been investigated by 1H NMR in both its primarily high-spin and its cyanide-inhibited low-spin forms. Both derivatives yield 1H NMR spectra indicative of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00187a033
更新日期:1994-05-31 00:00:00
abstract::The selective paramagnetic relaxation of oligonucleotide protons of d(GTGCAC)2 by delta- and lambda-Ni(phen)3(3+) and delta- and lambda-Cr(phen)3(3+) has been examined to obtain some structural insight into the noncovalent binding of tris(phenanthroline) metal complexes to DNA. The experiments demonstrate that the rel...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00459a007
更新日期:1990-02-20 00:00:00
abstract::The equilibrium between the rat brain tubulin alpha beta dimer and the dissociated alpha and beta monomers has been studied by analytical ultracentrifugation with use of a new method employing short solution columns, allowing rapid equilibration and hence short runs, minimizing tubulin decay. Simultaneous analysis of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00228a023
更新日期:1991-04-09 00:00:00
abstract::Following ribonuclease digestion of methyl-3H-labeled B77 avian sarcoma virus RNA subunits, methylated oligonucleotides were isolated by diethylaminoethylcellulose chromotogrpahy. Partial nucleotide sequences were deduced from the known enzymatic specificities of the ribonucleases. In addition to methylated nucleoside...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00622a021
更新日期:1977-02-08 00:00:00
abstract::The reduction of substrates catalyzed by nitrogenase utilizes an electron transfer (ET) chain comprised of three metalloclusters distributed between the two component proteins, designated as the Fe protein and the MoFe protein. The flow of electrons through these three metalloclusters involves ET from the [4Fe-4S] clu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi201003a
更新日期:2011-11-01 00:00:00
abstract::An assay was developed to study the spontaneous transfer and transbilayer movement (flip-flop) of lipid analogs labeled with the fluorescent fatty acid, 5-(5,7-dimethyl BODIPY)-1-pentanoic acid (C5-DMB-) in large unilamellar lipid vesicles comprised of 1-palmitoyl-2-oleoyl phosphatidylcholine (POPC). The assay is base...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi970145r
更新日期:1997-07-22 00:00:00
abstract::Binding of the N-acetyllactosamine-specific lectin from Erythrina corallodendron (ECorL) to four glycosphingolipids has been tested using the microtiter well assay. The role of several amino acids in the binding site region was studied by combining binding assays and molecular modeling for native and recombinant forms...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi962231h
更新日期:1997-04-15 00:00:00
abstract::To gain insight into the folding of large proteins, we constructed a bovine beta-lactoglobulin (beta-lg) dimeric mutant, A34C/C121A beta-lg. In the mutant, a free thiol group of wild-type beta-lg at Cys121 was removed and two beta-lg molecules were linked by a disulfide bridge through Cys34 created at the dimer's inte...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi8001715
更新日期:2008-06-03 00:00:00
abstract::Effects of eight differently substituted 1,4-benzoquinones (BQs) on the quantum yield of photoactivation of oxygen evolution (reconstitution of the Mn cluster) were examined with wheat photosystem II (PSII) membranes depleted of the Mn cluster by treatment with 1.0 mM NH2OH. Illumination with 10 flashes at 0.25-s inte...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00117a032
更新日期:1992-01-21 00:00:00
abstract::Catalase-peroxidases (KatGs) are unique bifunctional heme peroxidases with an additional posttranslationally formed redox-active Met-Tyr-Trp cofactor that is essential for catalase activity. On the basis of studies of bacterial KatGs, controversial mechanisms of hydrogen peroxide oxidation were proposed. The recent di...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00436
更新日期:2016-06-28 00:00:00
abstract::Because oxygen and selenium are in the same group (Family VI) in the periodic table, the site-specific mutagenesis at the atomic level by replacing RNA oxygen with selenium can provide insights on the structure and function of catalytic RNAs. We report here the first Se-derivatized ribozymes transcribed with all nucle...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi060455m
更新日期:2006-07-25 00:00:00
abstract::The mechanism by which newly synthesized sterols are transported from their site of synthesis, the endoplasmic reticulum (ER), to the sterol-enriched plasma membrane (PM) is not fully understood. Studies in mammalian cells suggest that newly synthesized cholesterol is transported to the PM in Golgi-bypassing vesicles ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi048296z
更新日期:2005-04-19 00:00:00
abstract::Unilamellar liposomes can be fused at low pH with the plasma membrane of cells that express the hemagglutinin glycoprotein of influenza virus on their surface [van Meer, G., & Simons, K. (1983) J. Cell Biol. 97, 1365-1374]. Here, we have resolved this fusion process into two kinetically distinct steps. The first and m...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00335a030
更新日期:1985-07-02 00:00:00
abstract::The modulatory action of Ca2+-calmodulin on multiple targets is inhibited by trifluoperazine, which competes with target proteins for calmodulin binding. The structure of calmodulin crystallized with two trifluoperazine molecules is determined by X-ray crystallography at 2.74 A resolution. The X-ray data together with...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi980795a
更新日期:1998-11-03 00:00:00
abstract::Photosynthetic water oxidation takes place at the Mn4CaO5 cluster in photosystem II. Around the Mn4CaO5 cluster, a hydrogen bond network is formed by several water molecules, including four water ligands. To clarify the role of this water network in the mechanism of water oxidation, we investigated the effects of the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b01120
更新日期:2016-01-26 00:00:00
abstract::The unfolded state and flexible linkers in the folded structure play essential roles in protein stability and folding and protein-protein interactions. Intrinsic to these roles is the fact that unfolded proteins and flexible linkers sample many different conformations. Polymer models may capture this and complement ex...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi036269n
更新日期:2004-03-02 00:00:00
abstract::Organisms and cellular systems which have adapted to stresses such as high temperature, desiccation, and urea-concentrating environments have responded by concentrating particular organic solutes known as osmolytes. These osmolytes are believed to confer protection to enzyme and other macromolecular systems against su...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00138a006
更新日期:1992-06-16 00:00:00
abstract::A 19-amino acid residue peptide, Gly-Trp-Leu-Lys-Ile-Lys-Ala-Ala-Met-Arg-Trp-Gly-Phe-Phe-Val-Arg-Lys-Lys- Ala, corresponding to the basic amphiphilic alpha-helix (BAA) motif at the C-terminus of a recombinant tobacco calmodulin-binding protein, TCB60, was synthesized. The interaction of the synthetic binding domain wi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9621674
更新日期:1997-02-25 00:00:00
abstract::The rapid phosphorylation and dephosphorylation of a variety of proteins downstream of the endothelin receptors A and B was investigated following stimulation of human lung fibroblasts with endothelin-1. Changes in the phosphorylation of proteins involved in the cell cycle, cytoskeleton, membrane channels, transcripti...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035414u
更新日期:2003-12-02 00:00:00
abstract::The C2 domain is a Ca2+-dependent, membrane-targeting motif originally discovered in protein kinase C and recently identified in numerous eukaryotic signal-transducing proteins, including cytosolic phospholipase A2 (cPLA2) of the vertebrate inflammation pathway. Intracellular Ca2+ signals recruit the C2 domain of cPLA...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9717340
更新日期:1997-10-07 00:00:00
abstract::The reduction of substrates catalyzed by nitrogenase normally requires nucleotide-dependent Fe protein delivery of electrons to the MoFe protein, which contains the active site FeMo cofactor. Here, it is reported that independent substitution of three amino acids (β-98(Tyr→His), α-64(Tyr→His), and β-99(Phe→His)) locat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00140
更新日期:2015-04-21 00:00:00
abstract::The nitrile stretching vibration is increasingly used as a sensitive infrared probe of local protein environments. However, site-specific incorporation of a nitrile moiety into proteins is difficult. Here we show that various aromatic nitriles can be easily incorporated into peptides and proteins via either thiol alky...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi101711a
更新日期:2010-12-14 00:00:00
abstract::Acyl carrier protein (ACP) is a cofactor in a variety of biosynthetic pathways, including fatty acid metabolism. Thus, it is of interest to determine structures of physiologically relevant ACP-fatty acid complexes. We report here the NMR solution structures of spinach ACP with decanoate (10:0-ACP) and stearate (18:0-A...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi052062d
更新日期:2006-04-25 00:00:00
abstract::The cytological architecture of the synaptonemal complex (SC), a meiosis-specific proteinaceous structure, is evolutionarily conserved among eukaryotes. However, little is known about the biochemical properties of SC components or the mechanisms underlying their roles in meiotic chromosome synapsis and recombination. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi4005528
更新日期:2013-08-06 00:00:00
abstract::Replication in archaea is carried out by proteins that are homologues of eukaryotic counterparts. However, the archaeal systems tend to be much simpler with fewer different genes encoding the core functions than in eukaryotic counterparts. In many archaea, there is a single minichromosome maintenance (MCM) homologue, ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi801479s
更新日期:2008-12-16 00:00:00
abstract::The fluorescence spectrum of C1 inhibitor (C1-Inh) in aqueous buffer has a maximum at 324 nm which shifts to 358 nm in 6.0 M guanidinium chloride (GdmC1), indicating that fluorescent tryptophans are buried in the native protein. When titrated with GdmC1, the fluorescence intensity, polarization, and emission maximum o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00331a025
更新日期:1985-05-07 00:00:00
abstract::Two highly conserved amino acid residues, an arginine and a glutamine, located near the C-terminal end of the gamma subunit, form a "catch" by hydrogen bonding with residues in an anionic loop on one of the three catalytic beta subunits of the bovine mitochondrial F1-ATPase [Abrahams, J. P., Leslie, A. G., Lutter, R.,...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi701581y
更新日期:2008-01-15 00:00:00
abstract::Beta-amylase (EC 3.2.1.2) is starch-hydrolyzing exo-type enzyme that can catalyze the successive liberation of beta-maltose from the nonreducing ends of alpha-1,4-linked glucopyranosyl polymers. There is a well-known phenomenon called multiple or repetitive attack where the enzyme releases several maltose molecules in...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061605w
更新日期:2007-01-23 00:00:00
abstract::Cytochrome P450BM-3 is a fatty acid monooxygenase that contains the catalytic P450 heme domain covalently attached to a diflavin P450 reductase domain. The function of the linker region connecting the C-terminal end of the heme domain to the N-terminal end of the reductase domain has been studied by deleting parts of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00035a031
更新日期:1995-09-05 00:00:00
abstract::We have studied a group of fusion peptides of influenza hemagglutinin in which the N-terminal amino acid, Gly (found in the wild-type peptide), has been systematically substituted with Ala, Ser, Val, or Glu. The activity of the intact hemagglutinin protein with these same substitutions has already been reported. As a ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0107187
更新日期:2001-07-31 00:00:00