Thermodynamics of reversible monomer-dimer association of tubulin.

abstract：:Conceptually one may envisage that substrate binding sites on the ABC transporter P-gp cycle between high- and low-affinity conformations in response to signals arising from nucleotide hydrolysis to effect active transport. A radioligand binding assay was used to characterize the interaction of [3H]vinblastine with P-...

journal_title：Biochemistry

authors： Martin C,Higgins CF,Callaghan R

更新日期：2001-12-25 00:00:00

abstract：:Acireductone dioxygenases (ARDs) are enzymes involved in the methionine recycle pathway, which regulates aspects of the cell cycle. Klebsiella pneumoniae produces two enzymes that share a common polypeptide sequence and differ only in the metal ion present. Reaction of acireductone (1,2-dihydroxy-3-keto-5-methylthiope...

journal_title：Biochemistry

authors： Al-Mjeni F,Ju T,Pochapsky TC,Maroney MJ

更新日期：2002-05-28 00:00:00

abstract：:The unfolded state and flexible linkers in the folded structure play essential roles in protein stability and folding and protein-protein interactions. Intrinsic to these roles is the fact that unfolded proteins and flexible linkers sample many different conformations. Polymer models may capture this and complement ex...

journal_title：Biochemistry

authors： Zhou HX

更新日期：2004-03-02 00:00:00

abstract：:To probe the structure and stability of the central region of lipid-free apolipoprotein (apo) A-I (residues 123-165), we studied the effects of four mutations made in this region on the conformation, stability, dimyristoylphosphatidylcholine (DMPC) binding kinetics, and size of discoidal reconstituted high-density lip...

journal_title：Biochemistry

authors： Gorshkova IN,Liu T,Zannis VI,Atkinson D

更新日期：2002-08-20 00:00:00

abstract：:The mechanisms that control N protein dependent antitermination in phage lambda have counterparts in many eukaryotic systems, including specific regulatory interactions of the antitermination protein with the nascent RNA transcript. Here we describe the specific and nonspecific RNA binding modes of antitermination pro...

journal_title：Biochemistry

authors： Van Gilst MR,Rees WA,Das A,von Hippel PH

更新日期：1997-02-11 00:00:00

abstract：:The reaction catalyzed by orotidine 5'-monophosphate decarboxylase (OMPDC) is accompanied by exceptional values for rate enhancement (k(cat)/k(non) = 7.1 × 10(16)) and catalytic proficiency [(k(cat)/K(M))/k(non) = 4.8 × 10(22) M(-1)]. Although a stabilized vinyl carbanion/carbene intermediate is located on the reactio...

journal_title：Biochemistry

authors： Iiams V,Desai BJ,Fedorov AA,Fedorov EV,Almo SC,Gerlt JA

更新日期：2011-10-04 00:00:00

abstract：:Pulsed electron paramagnetic resonance spectroscopy has been used to detect Mn(II)-ligand superhyperfine couplings in complexes with creatine kinase and in the Mn(II) metalloprotein concanavalin A. Electron spin-echo envelopes from Mn(II), bound in these complexes, are modulated by superhyperfine interactions between ...

journal_title：Biochemistry

authors： LoBrutto R,Smithers GW,Reed GH,Orme-Johnson WH,Tan SL,Leigh JS Jr

更新日期：1986-09-23 00:00:00

abstract：:N-Ethylmaleimide (NEM), which reacts readily with exposed sulfhydryl groups, has been shown to inhibit the activity of the microtubule (MT) motors kinesin, Ncd, and dynein. Currently, the mechanism of inhibition is not known for any of these proteins. To investigate the mechanism by which NEM inhibits Ncd, the recombi...

journal_title：Biochemistry

authors： Phelps KK,Walker RA

更新日期：1999-08-17 00:00:00

abstract：:Myc and Max belong to the b-HLH-LZ family of transcription factors. Heterodimerization between Myc and Max or homodimerization of Max allows these proteins to bind their cognate DNA sequence known as the E-box (CACGTG). Recent evidence has suggested that the c-Myc/Max heterodimeric b-HLH-LZ could interact to form a he...

journal_title：Biochemistry

authors： Lebel R,McDuff FO,Lavigne P,Grandbois M

更新日期：2007-09-11 00:00:00

abstract：:Solvent proton nuclear magnetic dispersion studies at 25, 100, and 300 MHz have been performed on protocatechuate 3,4-dioxygenase (PCD) and its complexes with 3-chloro-4-hydroxybenzoate and 3-fluoro-4-hydroxybenzoate. Longitudinal and transverse relaxation rates were measured for these compounds and for the apoenzyme....

journal_title：Biochemistry

authors： Felton RH,Gordon SL,Sowell AL,May SW

更新日期：1984-08-14 00:00:00

abstract：:The heat-stable, protein inhibitor of the cyclic adenosine monophosphate (cAMP) dependent protein kinase [Walsh, D. A., Ashby, C. D., Gonzalez, C., Calkins, D., Fischer, E., & Krebs, E (1971a) J. Biol. Chem. 246, 1977-1985] has been purified to homogeneity from rabbit skeletal muscle by preparative electrophoresis. Em...

journal_title：Biochemistry

authors： McPherson JM,Whitehouse S,Walsh DA

更新日期：1979-10-30 00:00:00

abstract：:The instantaneous electrochromic response of carotenoids associated with the B800-850 light-harvesting complex of Rhodobacter sphaeroides has been used widely as an intrinsic probe of membrane potential. In the present study, the structural basis for this phenomenon was examined by phospholipase A2 digestion of chroma...

journal_title：Biochemistry

authors： Olivera LM,Niederman RA

更新日期：1993-01-26 00:00:00

abstract：:X-ray studies of the proofreading 3',5'-exonuclease site of the large (Klenow) fragment of DNA polymerase I have detected a binuclear metal complex consisting of a pentacoordinate metal (site A) which shares a ligand, Asp-355, with an octahedral metal (site B) [Freemont, P. S., Friedman, J. M., Beese, L. S., Sanderson...

journal_title：Biochemistry

authors： Han H,Rifkind JM,Mildvan AS

更新日期：1991-11-19 00:00:00

abstract：:The temperature dependence of the packing (order) and fluidity (microviscosity) of rabbit small, intestinal brush border vesicle membranes and of liposomes made from their extracted lipids has been investigated by using a variety of lipid spin probes. The lipids in the brush border membrane are present essentially as ...

journal_title：Biochemistry

authors： Hauser H,Gains N,Semenza G,Spiess M

更新日期：1982-10-26 00:00:00

abstract：:Phosphatidylserine (PS) in the plasma membrane of nonactivated human platelets is almost entirely located on the cytoplasmic side. Stimulation of platelets with the Ca2+ ionophore A23187 or combined action of collagen plus thrombin results in a rapid loss of the asymmetric distribution of PS. Also, treatment with the ...

journal_title：Biochemistry

authors： Bevers EM,Tilly RH,Senden JM,Comfurius P,Zwaal RF

更新日期：1989-03-21 00:00:00

abstract：:The PRL (phosphatase of regenerating liver) phosphatases represent a distinct class of protein tyrosine phosphatases, which are implicated in tumorigenesis and metastasis processes. Accumulating evidence indicates that alteration of PRL1 expression affects cell motility and tumor metastasis, although the biochemical p...

journal_title：Biochemistry

authors： Luo Y,Liang F,Zhang ZY

更新日期：2009-03-03 00:00:00

abstract：:Locked nucleic acid (LNA) is a nucleic acid analogue containing one or more LNA nucleotide monomers with a bicyclic furanose unit locked in an RNA mimicking sugar conformation. LNA oligonucleotides display unprecedented hybridization affinity toward complementary single-stranded RNA and complementary single- or double...

journal_title：Biochemistry

authors： Vester B,Wengel J

更新日期：2004-10-26 00:00:00

abstract：:The bacterial alpha-lytic protease (alphaLP) is synthesized as a precursor containing a large N-terminal pro region (Pro) transiently required for correct folding of the protease [Silen, J. L., and Agard, D. A. (1989) Nature 341, 462-464]. Upon folding, the precursor is autocatalyticly cleaved to yield a tight-binding...

journal_title：Biochemistry

authors： Anderson DE,Peters RJ,Wilk B,Agard DA

更新日期：1999-04-13 00:00:00

abstract：:Multiple forms of the acute-phase serum protein SAA were isolated from the lipoprotein fraction of plasma from a single individual. These protein forms were purified by size-exclusion, ion-exchange, and reverse-phase high-pressure liquid chromatography, and then the tryptic peptides were subjected to amino acid sequen...

journal_title：Biochemistry

authors： Dwulet FE,Wallace DK,Benson MD

更新日期：1988-03-08 00:00:00

abstract：:Determining the sequence of bond cleavages, and consequently the nature of intermediates, in enzyme-catalyzed reactions is a major goal of mechanistic enzymology. When significant primary isotope effects on V/K are observed for two different bond cleavages, both bonds may be broken in the same transition state or they...

journal_title：Biochemistry

authors： Bahnson BJ,Anderson VE

更新日期：1991-06-18 00:00:00

abstract：:Type II polyketides are a class of natural products that include pharmaceutically important aromatic compounds such as the antibiotic tetracycline and antitumor compound doxorubicin. The type II polyketide synthase (PKS) is a complex consisting of 5-10 standalone domains homologous to fatty acid synthase (FAS). Polyke...

journal_title：Biochemistry

authors： Korman TP,Tan YH,Wong J,Luo R,Tsai SC

更新日期：2008-02-19 00:00:00

abstract：:Pyruvate phosphate dikinase catalyzes the interconversion of adenosine 5'-triphosphate (ATP), orthophosphate (P(i)), and pyruvate with adenosine 5'-monophosphate (AMP), pyrophosphate (PP(i)), and phosphoenolpyruvate (PEP). The Arg 561 residue of Clostridium symbiosum PPDK is contained within a Gly-rich stretch of sequ...

journal_title：Biochemistry

authors： Yankie L,Xu Y,Dunaway-Mariano D

更新日期：1995-02-21 00:00:00

abstract：:Casein kinase II is a widely distributed protein serine/threonine kinase. The holoenzyme appears to be a tetramer, containing two alpha or alpha' subunits (or one of each) and two beta subunits. Complementary DNA clones encoding the subunits of casein kinase II were isolated from a human T-cell lambda gt10 library usi...

journal_title：Biochemistry

authors： Lozeman FJ,Litchfield DW,Piening C,Takio K,Walsh KA,Krebs EG

更新日期：1990-09-11 00:00:00

abstract：:An S3 electron paramagnetic resonance (EPR) signal is observed in a variety of photosystem 2 (PS2) samples in which the oxygen-evolving complex (OEC) has been inhibited. These signals have been proposed to be due to an interaction, S2X+, between the manganese cluster in an oxidation state equivalent to S2 and an organ...

journal_title：Biochemistry

authors： MacLachlan DJ,Nugent JH

更新日期：1993-09-21 00:00:00

abstract：:Acarbose is a naturally occurring pseudo-tetrasaccharide. It has been used in conjunction with other drugs in the treatment of diabetes where it acts as an inhibitor of intestinal glucosidases. To probe the interactions of acarbose with other carbohydrate recognition enzymes, the crystal structure of E. coli maltodext...

journal_title：Biochemistry

authors： O'Reilly M,Watson KA,Johnson LN

更新日期：1999-04-27 00:00:00

abstract：:The multimeric cytochromes described to date in sulfate- and sulfur-reducing bacteria are associated with diverse respiratory modes involving the use of elemental sulfur or oxidized sulfur compounds as terminal acceptors. They exhibit no structural similarity with the other cytochrome c classes and are characterized b...

journal_title：Biochemistry

authors： Bruschi M,Woudstra M,Guigliarelli B,Asso M,Lojou E,Petillot Y,Abergel C

更新日期：1997-09-02 00:00:00

abstract：:Rett syndrome causing missense mutations in the methyl-CpG-binding domain (MBD) of methyl CpG-binding protein 2 (MeCP2) were investigated both in silico and in vitro to reveal their effect on protein stability. It is demonstrated that the vast majority of frequently occurring mutations in the human population indeed a...

journal_title：Biochemistry

authors： Kucukkal TG,Yang Y,Uvarov O,Cao W,Alexov E

更新日期：2015-10-20 00:00:00

abstract：:The circadian clock allows organisms to accurately predict the earth's rotation and modify their behavior as a result. Genetic analyses in a variety of organisms have defined a mechanism based largely on gene expression feedback loops. However, as we delve more deeply into the mechanisms of circadian timekeeping, we a...

journal_title：Biochemistry

authors： Hoyle NP,O'Neill JS

更新日期：2015-01-20 00:00:00

abstract：:The aryl hydrocarbon receptor (AhR) is an intracellular receptor protein that regulates gene transcription in response to both man-made and natural ligands. A modular transactivaton domain (TAD) has been mapped to the 304 C-terminal amino acids and consists of acidic, Q-rich, and P/S/T-rich subdomains. We have used st...

journal_title：Biochemistry

authors： Watt K,Jess TJ,Kelly SM,Price NC,McEwan IJ

更新日期：2005-01-18 00:00:00

abstract：:The NikR transcription factor from Escherichia coli is a Ni(II)-dependent repressor that regulates production of the nickel ion transporter encoded by the nik operon. In the previous paper in this issue (Wang, S. C., Dias, A., Bloom, S. L., and Zamble, D. B. (2004) Selectivity of Metal Binding and Metal-Induced Stabil...

journal_title：Biochemistry

authors： Bloom SL,Zamble DB

更新日期：2004-08-10 00:00:00