Abstract:
:An S3 electron paramagnetic resonance (EPR) signal is observed in a variety of photosystem 2 (PS2) samples in which the oxygen-evolving complex (OEC) has been inhibited. These signals have been proposed to be due to an interaction, S2X+, between the manganese cluster in an oxidation state equivalent to S2 and an organic radical, either oxidized histidine [Boussac et al. (1990) Nature 347, 303-306] or the tyrosine radical Yz+ [Hallahan et al. (1992) Biochemistry 31, 4562-4573]. We report that treatment of PS2 with acetate at pH 5.5 leads to a slowing of the reduction of Yz+ and allows the trapping of an S3-type state on freezing to 77 K following illumination at 277 K. The S3 EPR signal in acetate-treated PS2 has a broader and more complex line shape but otherwise has similar properties to other S3 signals. The addition to acetate-treated samples in the S1 state of the herbicide 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), which allows only a single turnover of the reaction center, causes a large reduction in the yield of the S3 signal. Various anion and cation treatments change the S3 signal line shape and are used to show that acetate probably acts by binding and displacing chloride. We propose that a variety of treatments which affect calcium and chloride cofactor binding cause a modification of the S2 state of the manganese cluster, slow the reduction of Yz+, and allow an S3 EPR signal to be observed following illumination.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Biochemistryjournal_title
Biochemistryauthors
MacLachlan DJ,Nugent JHdoi
10.1021/bi00088a032subject
Has Abstractpub_date
1993-09-21 00:00:00pages
9772-80issue
37eissn
0006-2960issn
1520-4995journal_volume
32pub_type
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