Abstract:
:Following ribonuclease digestion of methyl-3H-labeled B77 avian sarcoma virus RNA subunits, methylated oligonucleotides were isolated by diethylaminoethylcellulose chromotogrpahy. Partial nucleotide sequences were deduced from the known enzymatic specificities of the ribonucleases. In addition to methylated nucleosides in the 5'-terminal cap structure, m7G(5')GmpCp, N6-methyladenosine(m6A) was found to be present in only two internal sequences of the RNA molecule, Gpm6ApC and Apm6ApC. The average numbers of methylated nucleosides per RNA subunit are about 12-13 in Gpm6ApC, 1-2 in Apm6ApC, and 2 in m7GpppGmpCp. The sequences containing m6A in B77 sarcoma virus RNA are identical to m6A-containing sequences previously reported for the bulk mRNA from HeLa cells (Wei, C.M., Gershowitz, A., and Moss, B. (1976), Biochemistry 15, 397-401). Analysis of the oligonucleotides produced by RNase A digestion indicated that the sequence of bases on the 5' side of these trinucleotides is not specific. The oligonucleotide profile, however, was highly reproducible in different virus preparations. This suggests that the methylations occur at specific positions on the RNA molecule. Some of the methylated oligonucleotides produced by RNase A digestion appear to be present in less than molar amounts. Several hypotheses are proposed to explain this result.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Dimock K,Stoltzfus CMdoi
10.1021/bi00622a021subject
Has Abstractpub_date
1977-02-08 00:00:00pages
471-8issue
3eissn
0006-2960issn
1520-4995journal_volume
16pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The kinetics of the interconversions of the subgel and gel phases in dipalmitoylphosphatidylcholine have been studied by using differential dilatometry, differential scanning calorimetry (DSC), and neutral buoyancy centrifugation as a function of incubation temperature and deuteriation of the solvent. As seen by other...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00388a016
更新日期:1987-07-14 00:00:00
abstract::Stopped-flow kinetic measurements of the association of filipin with sterols in intact cells and isolated membranes of Mycoplasma capricolum were used to study the effects of varying the phospholipid in the membrane. The phospholipid composition and content of the membrane were varied by growing cells in an albumin-co...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00511a019
更新日期:1981-04-14 00:00:00
abstract::Phosphatidic (PtdOH) acid formation is recognized as an important step in numerous signaling pathways in both plants and mammals. To study the role of this lipid in signaling pathways, it is of major interest to be able to increase the amount of this lipid directly. Therefore, "caged" PtdOH was synthesized, which rele...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0351460
更新日期:2004-04-13 00:00:00
abstract::Characterization of the complete gene sequence encoding the alpha-galactosidase from Phanerochaete chrysosporium confirms that this enzyme is a member of glycosyl hydrolase family 27 [Henrissat, B., and Bairoch, A. (1996) Biochem. J. 316, 695-696]. This family, together with the family 36 alpha-galactosidases, forms g...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0008074
更新日期:2000-08-15 00:00:00
abstract::RNA pur*pur-pyr and pyr*pur-pyr (pur = purine, pyr = pyrimidine) triple helices consisting of a Watson-Crick base-paired 28mer hairpin duplex and a Hoogsteen base-paired purine or pyrimidine 12mer are targeted with photoactivated cleavage by the metal complex Rh(phen)2phi3+ (phen = phenanthroline, phi = 9, 10-phenanth...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi980509v
更新日期:1998-06-23 00:00:00
abstract::The solution structure of an alpha-insect toxin from Buthus martensii Karsch, BmKalphaIT01, has been determined by two-dimensional NMR spectroscopy and molecular modeling techniques. Combining the sequence homology comparison and toxicity bioassays, BmKalphaIT01 has been suggested to be a natural mutant of alpha-insec...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi7006788
更新日期:2007-10-09 00:00:00
abstract::The pH dependence of the transient aerobic kinetics of cytochromes c and a has been investigated with cytochrome oxidase reconstituted in phospholipid vesicles in the absence and presence of an uncoupler and an ionophore. The cytochrome a reduction level immediately after the burst phase was 60-80% and was not signifi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00434a007
更新日期:1989-04-18 00:00:00
abstract::The biosynthesis of the antitumor antibiotic sibiromycin by Streptosporangium sibiricum requires the construction of four units: the amino sugar from glucose; the anthranilate ring from DL-tryptophan probably via kynurenine; the aromatic methyl group from methionine; the propylidene proline from L-tyrosine with the lo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00586a029
更新日期:1979-09-18 00:00:00
abstract::Ferredoxin I from Desulfovibrio africanus (Da FdI) is a small acidic [4Fe-4S] cluster protein that exchanges electrons with pyruvate-ferredoxin oxidoreductase (PFOR), a key enzyme in the energy metabolism of anaerobes. The thermodynamic properties and the electron transfer between PFOR and either native or mutated FdI...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0485878
更新日期:2004-12-14 00:00:00
abstract::Heme oxygenases convert heme to free iron, CO, and biliverdin. Saccharomyces cerevisiae and Candida albicans express putative heme oxygenases that are required for the acquisition of iron from heme, a critical process for fungal survival and virulence. The putative heme oxygenases Hmx1 and CaHmx1 from S. cerevisiae an...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061429r
更新日期:2006-12-12 00:00:00
abstract::Several phosphatidylinositol 4,5-bisphosphate (PtdInsP2)-regulated actin-binding proteins and most phosphoinositide-specific phospholipases C (PI-PLCs) comprise a basic amino acid motif (KxxxKxKK, where x denotes any amino acid), which was previously suggested to represent a PtdInsP2-binding site commonly present in t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00015a023
更新日期:1995-04-18 00:00:00
abstract::The derivatives of calf thymus DNA in which n-butylamine is covalently attached as described in the preceding paper in this series [Chen, C. Y., Pheiffer, B. H., Zimmerman, S. B., & Hanlon, S. (1983) Biochemistry (preceding paper in this issue)] were examined by Raman spectroscopy. As previously mentioned, these compl...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00289a021
更新日期:1983-09-27 00:00:00
abstract::The reduction of D-amino acid oxidase (DAAO) by hydrated electrons (eaq-) has been studied in the absence and presence of benzoate by pulse radiolysis. The eaq-did not reduce the flavin moiety in DAAO and reacted with the amino acid residues in the protein. In the presence of benzoate, eaq- first reacted with benzoate...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00278a028
更新日期:1983-04-26 00:00:00
abstract::A debilitating complication of long-term hemodialysis is the deposition of beta-2-microglobulin (beta2m) as amyloid plaques in the joint space. We have recently shown that Cu(2+) can be a contributing, if not causal, factor at concentrations encountered during dialysis therapy. The basis for this effect is destabiliza...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi025944a
更新日期:2002-08-27 00:00:00
abstract::It is known that aconitase from mammalian mitochondria is only partially active as isolated but may be activated by incubation with iron, ascorbate, and a thiol, or with dithionite. It has been suggested that the added Fe in the activation mixture is essential for activation and that it is incorporated in the enzyme [...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00529a023
更新日期:1981-12-22 00:00:00
abstract::Phospholipids containing a saturated fatty acid in the primary position and an unsaturated fatty acid in the secondary position are a major structural part of biological membranes. The mixed-chain hydrophobic core of the membranes is the diacylglycerol part. To better understand the core properties of membranes we hav...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00051a015
更新日期:1995-12-26 00:00:00
abstract::NahE and PhdJ are bifunctional hydratase-aldolases in bacterial catabolic pathways for naphthalene and phenanthrene, respectively. Bacterial species with these pathways can use polycyclic aromatic hydrocarbons (PAHs) as sole sources of carbon and energy. Because of the harmful properties of PAHs and their widespread d...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b00532
更新日期:2018-06-26 00:00:00
abstract::An S3 electron paramagnetic resonance (EPR) signal is observed in a variety of photosystem 2 (PS2) samples in which the oxygen-evolving complex (OEC) has been inhibited. These signals have been proposed to be due to an interaction, S2X+, between the manganese cluster in an oxidation state equivalent to S2 and an organ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00088a032
更新日期:1993-09-21 00:00:00
abstract::A recombinant system was developed for generation of steroid-receptor complexes in vitro. The DNA- and steroid-binding domains of the rat mineralocorticoid receptor were expressed in Escherichia coli as a fusion protein with glutathione S-transferase. The identity of the expressed recombinant protein was confirmed by ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00084a028
更新日期:1993-08-24 00:00:00
abstract::Mutations in the Kirsten ras (KRAS) gene are present in almost all pancreatic adenocarcinomas, and one common mutation is at codon 12: GGT (Gly) is transformed into GAT (Asp). In this work we have targeted the KRAS coding sequence embracing the GAT mutation with a sense PNA molecule (P14), with the aim of downregulati...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0505215
更新日期:2005-08-09 00:00:00
abstract::Voltage-dependent L-type calcium (Ca) channels are heteromultimeric proteins that are regulated through phosphorylation by cAMP-dependent protein kinase (PKA). We demonstrated that the beta 2 subunit was a substrate for PKA in intact cardiac myocytes through back-phosphorylation experiments. In addition, a heterologou...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990896o
更新日期:1999-08-10 00:00:00
abstract::High-resolution proton nuclear magnetic resonance (1H NMR) has been used to study the conformation and dynamics of biosynthetic analogues of actinomycin D that have been substituted at one or both of the 3'-amino acids with azetidine-2-carboxylic acid, pipecolic acid, or 4-ketoproline for one or both of the prolines. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00540a007
更新日期:1982-05-25 00:00:00
abstract::Isothiouronium compounds [Hoving, S., Bar-Shimon, M., Tijmes, J. J., Goldshleger, R., Tal, D. M., and Karlish, S. J. (1995) J. Biol. Chem. 270, 29788-29793] act as high-affinity competitive antagonists for Na(+) and K(+) (Rb(+)) on the renal Na(+)/K(+)-ATPase where they favor the E1 conformation. We have now character...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi026071n
更新日期:2003-04-01 00:00:00
abstract::The stability of three forms of glucoamylase from Aspergillus niger has been investigated by differential scanning and isothermal titration calorimetry: Glucoamylase 1 (GA1), which consists of a catalytic domain and a starch-binding domain (SBD) connected by a heavily O-glycosylated linker region; glucoamylase 2 (GA2)...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990185q
更新日期:1999-05-11 00:00:00
abstract::The preparation of native alpha- and beta-chains of carp hemoglobin and the preparation of the hybrid hemoglobin alpha(carp):beta(human), hybrid I, are described. CO association and dissociation kinetics were determined for the hybrid hemoglobin alpha(carp):beta(human) as a function of fractional saturation by stopped...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00305a011
更新日期:1984-05-08 00:00:00
abstract::First identified on the surface of lymphoids as a type II transmembrane protein, CD38 has now been established to have dual functions not only as a receptor but also as a multifunctional enzyme,catalyzing the synthesis of and hydrolysis of a general calcium messenger molecule, cyclic ADP-ribose(cADPR). The receptorial...
journal_title:Biochemistry
pub_type: 杂志文章
doi:
更新日期:2008-12-30 00:00:00
abstract::Redox transitions in the Rhodobacter capsulatus cytochrome bc(1) complex were investigated by perfusion-induced attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy combined with synchronous visible spectroscopy, in both the wild type and a cytochrome c(1) point mutant, M183K, in which the mi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049211x
更新日期:2004-07-27 00:00:00
abstract::Absorbance difference spectra were recorded at 20 degrees C from 30 ns to milliseconds after photolysis of lauryl maltoside suspensions of artificial visual pigments derived from 9-cis isomers of 5-ethylretinal, 8,16-methanoretinal (a 6-s-trans-bicyclic analogue), or 5-demethyl-8-methylretinal. In all three pigments, ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011461f
更新日期:2002-02-12 00:00:00
abstract::The membrane-bound quinoprotein glucose dehydrogenase (mGDH) in Escherichia coli contains pyrroloquinoline quinone (PQQ) and participates in the direct oxidation of D-glucose to D-gluconate by transferring electrons to ubiquinone (UQ). To elucidate the mechanism of ubiquinone reduction by mGDH, we applied a pulse radi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi051347n
更新日期:2005-10-18 00:00:00
abstract::In clostridial-type ferredoxins, each of the two [4Fe-4S]2+/+ clusters receives three of its four ligands from a CysXXCysXXCys motif. Azotobacter vinelandii ferredoxin I (AvFdI) is a seven-iron ferredoxin that contains one [4Fe-4S]2+/+ cluster and one [3Fe-4S]+/0 cluster. During the evolution of the 7Fe azotobacter-ty...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9810499
更新日期:1998-09-15 00:00:00