Sequence specificity of internal methylation in B77 avian sarcoma virus RNA subunits.


:Following ribonuclease digestion of methyl-3H-labeled B77 avian sarcoma virus RNA subunits, methylated oligonucleotides were isolated by diethylaminoethylcellulose chromotogrpahy. Partial nucleotide sequences were deduced from the known enzymatic specificities of the ribonucleases. In addition to methylated nucleosides in the 5'-terminal cap structure, m7G(5')GmpCp, N6-methyladenosine(m6A) was found to be present in only two internal sequences of the RNA molecule, Gpm6ApC and Apm6ApC. The average numbers of methylated nucleosides per RNA subunit are about 12-13 in Gpm6ApC, 1-2 in Apm6ApC, and 2 in m7GpppGmpCp. The sequences containing m6A in B77 sarcoma virus RNA are identical to m6A-containing sequences previously reported for the bulk mRNA from HeLa cells (Wei, C.M., Gershowitz, A., and Moss, B. (1976), Biochemistry 15, 397-401). Analysis of the oligonucleotides produced by RNase A digestion indicated that the sequence of bases on the 5' side of these trinucleotides is not specific. The oligonucleotide profile, however, was highly reproducible in different virus preparations. This suggests that the methylations occur at specific positions on the RNA molecule. Some of the methylated oligonucleotides produced by RNase A digestion appear to be present in less than molar amounts. Several hypotheses are proposed to explain this result.






Dimock K,Stoltzfus CM




Has Abstract


1977-02-08 00:00:00












  • Amplitude analysis of single-wavelength time-dependent absorption data does not support the conventional sequential mechanism for the reduction of dioxygen to water catalyzed by bovine heart cytochrome c oxidase.

    abstract::The reactions of fully reduced and mixed-valence bovine heart cytochrome c oxidase with dioxygen have been reinvestigated in the absence and presence of metal ions (Zn(2+), Ni(2+), and Cd(2+)) by time-resolved optical absorption spectroscopy using the CO flow-flash technique. The time-resolved data were recorded on a ...


    pub_type: 杂志文章


    authors: Szundi I,Cappuccio J,Einarsdóttir O

    更新日期:2004-12-21 00:00:00

  • Comparison of the nerve growth factor proteins from cobra venom (Naja naja) and mouse submaxillary gland.

    abstract::The nerve growth factors (NGF's) isolated from cobra venom (Naja naja) and mouse submaxillary gland are closely related proteins. They are structurally similar in that about 60% of their amino acid residues are identical (Hogue-Angeletti, R. A., Frazier, W. A., Jacobs, J. W., Niall, H.D., and Bradshaw, R. A. (1976), B...


    pub_type: 杂志文章


    authors: Server AC,Herrup K,Shooter EM,Hogue-Angeletti RA,Frazier WA,Bradshaw RA

    更新日期:1976-01-13 00:00:00

  • Substrate specificity of homogeneous monkeypox virus uracil-DNA glycosylase.

    abstract::Weak or nonexistent smallpox immunity in today's human population raises concerns about the possibility of natural or provoked genetic modifications leading to re-emergence of variola virus and other poxviruses. Thus, the development of new antiviral strategies aimed at poxvirus infections in humans is a high priority...


    pub_type: 杂志文章


    authors: Duraffour S,Ishchenko AA,Saparbaev M,Crance JM,Garin D

    更新日期:2007-10-23 00:00:00

  • Substrate-Induced Facilitated Dissociation of the Competitive Inhibitor from the Active Site of O-Acetyl Serine Sulfhydrylase Reveals a Competitive-Allostery Mechanism.

    abstract::By classical competitive antagonism, a substrate and competitive inhibitor must bind mutually exclusively to the active site. The competitive inhibition of O-acetyl serine sulfhydrylase (OASS) by the C-terminus of serine acetyltransferase (SAT) presents a paradox, because the C-terminus of SAT binds to the active site...


    pub_type: 杂志文章


    authors: Singh AK,Ekka MK,Kaushik A,Pandya V,Singh RP,Banerjee S,Mittal M,Singh V,Kumaran S

    更新日期:2017-09-19 00:00:00

  • Human frataxin activates Fe-S cluster biosynthesis by facilitating sulfur transfer chemistry.

    abstract::Iron-sulfur clusters are ubiquitous protein cofactors with critical cellular functions. The mitochondrial Fe-S assembly complex, which consists of the cysteine desulfurase NFS1 and its accessory protein (ISD11), the Fe-S assembly protein (ISCU2), and frataxin (FXN), converts substrates l-cysteine, ferrous iron, and el...


    pub_type: 杂志文章


    authors: Bridwell-Rabb J,Fox NG,Tsai CL,Winn AM,Barondeau DP

    更新日期:2014-08-05 00:00:00

  • Electrostatic guidance of glycosyl cation migration along the reaction coordinate of uracil DNA glycosylase.

    abstract::The DNA repair enzyme uracil DNA glycosylase has been crystallized with a cationic 1-aza-2'-deoxyribose-containing DNA that mimics the ultimate transition state of the reaction in which the water nucleophile attacks the anomeric center of the oxacarbenium ion-uracil anion reaction intermediate. Comparison with substra...


    pub_type: 杂志文章


    authors: Bianchet MA,Seiple LA,Jiang YL,Ichikawa Y,Amzel LM,Stivers JT

    更新日期:2003-11-04 00:00:00

  • Complete amino acid sequence of human liver cytosolic alanine aminotransferase (GPT) determined by a combination of conventional and mass spectral methods.

    abstract::The complete amino acid sequence of human liver cytosolic alanine aminotransferase (GPT) (EC is presented. Two primary sets of overlapping fragments were obtained by cleavage of the pyridylethylated protein at methionyl and lysyl bonds with cyanogen bromide and Achromobacter protease I, respectively. Isolated...


    pub_type: 杂志文章


    authors: Ishiguro M,Takio K,Suzuki M,Oyama R,Matsuzawa T,Titani K

    更新日期:1991-10-29 00:00:00

  • Clustered negative charges on the lipid membrane surface induce beta-sheet formation of prion protein fragment 106-126.

    abstract::The conformational conversion of prion protein (PrP) from an alpha-helix-rich normal cellular isoform (PrPC) to a beta-sheet-rich pathogenic isoform (PrPSc) is a key event in the development of prion diseases, and it takes place in caveolae, cavelike invaginations of the plasma membrane. A peptide homologous to residu...


    pub_type: 杂志文章


    authors: Miura T,Yoda M,Takaku N,Hirose T,Takeuchi H

    更新日期:2007-10-16 00:00:00

  • Exploring the role and the binding affinity of a second zinc equivalent in B. cereus metallo-beta-lactamase.

    abstract::Metallo-beta-lactamases are a newly characterized family of zinc enzymes present in several pathogenic strains that represent an emerging clinical threat. Enzymes from different organisms exhibit an outstanding functional diversity, particularly in the metal ion requirements for activity. We have investigated the effe...


    pub_type: 杂志文章


    authors: Rasia RM,Vila AJ

    更新日期:2002-02-12 00:00:00

  • Identification of critical lysyl residues in the pyrophosphate-dependent phosphofructo-1-kinase of Propionibacterium freudenreichii.

    abstract::Pyrophosphate-dependent 6-phosphofructo-1-kinase (PPi-PFK) from Propionibacterium freudenreichii was inactivated by low concentrations of the lysine-specific reagent pyridoxal phosphate (PLP) after sodium borohydride reduction. The substrates fructose 6-phosphate and fructose 1,6-bisphosphate protected against inactiv...


    pub_type: 杂志文章


    authors: Green PC,Latshaw SP,Ladror US,Kemp RG

    更新日期:1992-05-26 00:00:00

  • Carbon-13 nuclear magnetic resonance studies on the interaction of glycophorin with lecithin in reconstituted vesicles.

    abstract::Glycophorin, the MN blood group substance, is a major intrinsic glycoprotein in erythrocyte membranes. The interaction of glycophorin with phosphatidylcholine, 13C-labeled in specific positions in reconstituted unilamellar vesicles, was investigated by using the 13C NMR technique. 1-Palmitoyl-2-([14-13C]linoleoyl)-sn-...


    pub_type: 杂志文章


    authors: Utsumi H,Tunggal BD,Stoffel W

    更新日期:1980-05-27 00:00:00

  • Mode of interaction of aminooxy compounds with sheep liver serine hydroxymethyltransferase.

    abstract::The interaction of aminooxy compounds such as aminooxyacetate (AAA), L-canaline, and hydroxylamine with sheep liver serine hydroxymethyltransferase (EC was studied by absorption spectra and stopped-flow spectrophotometry and compared with the unique feature of interaction of O-amino-D-serine (OADS) with the e...


    pub_type: 杂志文章


    authors: Baskaran N,Prakash V,Savithri HS,Radhakrishnan AN,Appaji Rao N

    更新日期:1989-12-12 00:00:00

  • Purification and characterization of a transmembrane domain-deleted form of lecithin retinol acyltransferase.

    abstract::Lecithin retinol acyltransferase (LRAT) catalyzes the esterification of all-trans-retinol into all-trans-retinyl ester, an essential reaction in the vertebrate visual cycle. Since all-trans-retinyl esters are the substrates for the isomerization reaction that generates 11-cis-retinoids, this esterification reaction is...


    pub_type: 杂志文章


    authors: Bok D,Ruiz A,Yaron O,Jahng WJ,Ray A,Xue L,Rando RR

    更新日期:2003-05-27 00:00:00

  • Synthetic charybdotoxin-iberiotoxin chimeric peptides define toxin binding sites on calcium-activated and voltage-dependent potassium channels.

    abstract::Charybdotoxin (ChTX) and iberiotoxin (IbTX) are highly charged peptidyl toxins which exhibit 68% sequence identity and share a similar three-dimensional structure. Despite these structural similarities, IbTX and ChTX differ in their selectivity for two types of potassium channels; large conductance calcium-activated p...


    pub_type: 杂志文章


    authors: Giangiacomo KM,Sugg EE,Garcia-Calvo M,Leonard RJ,McManus OB,Kaczorowski GJ,Garcia ML

    更新日期:1993-03-09 00:00:00

  • A family of leukemia inhibitory factor-binding peptides that can act as antagonists when conjugated to poly(ethylene glycol).

    abstract::A panel of six naïve 14-residue random peptide libraries displayed polyvalently on M13 phage was pooled and sorted against human leukemia inhibitory factor (LIF). After four rounds of selection, a single large family of peptides with the consensus sequence XCXXXXG(A/S)(D/E)(W/F)WXCF was found to bind specifically to L...


    pub_type: 杂志文章


    authors: Fairlie WD,Uboldi AD,Hemmings GJ,Smith BJ,Martin HM,Morgan PO,Baca M

    更新日期:2003-11-18 00:00:00

  • Infrared spectroscopy of polynucleotides in the carbonyl region in H2O solution: A.U systems.

    abstract::Strong absorbance of water at 1645 cm-1 has previously prevented dependable observation of infrared spectra of H2O solutions in the carbonyl region. We have obtained reliable spectra in pure water and in alcohol-water mixtures in this region and used them to study A.U polynucleotide interaction. Spectra in pure water ...


    pub_type: 杂志文章


    authors: Miles HT,Frazier J

    更新日期:1978-07-11 00:00:00

  • Polymer models of protein stability, folding, and interactions.

    abstract::The unfolded state and flexible linkers in the folded structure play essential roles in protein stability and folding and protein-protein interactions. Intrinsic to these roles is the fact that unfolded proteins and flexible linkers sample many different conformations. Polymer models may capture this and complement ex...


    pub_type: 杂志文章


    authors: Zhou HX

    更新日期:2004-03-02 00:00:00

  • Identification and characterization of O-acetylpeptidoglycan esterase: a novel enzyme discovered in Neisseria gonorrhoeae.

    abstract::Modification of the bacterial cell wall heteropolymer peptidoglycan by addition of an acetyl group to the C-6 hydroxyl group of N-acetylmuramoyl residues is known to inhibit the activity of muramidases (lysozymes) of innate immune systems. The O-acetylation of peptidoglycan also precludes the action of intrinsic lytic...


    pub_type: 杂志文章


    authors: Weadge JT,Clarke AJ

    更新日期:2006-01-24 00:00:00

  • Sequence-selective binding of amiloride to DNA.

    abstract::Nuclease footprinting techniques have been employed to investigate the interaction between the diuretic drug amiloride, a sodium channel blocker with potential therapeutic use in the treatment of cystic fibrosis, and three DNA fragments of defined sequence. Using either DNAse I or micrococcal nuclease as probes, an un...


    pub_type: 杂志文章


    authors: Bailly C,Cuthbert AW,Gentle D,Knowles MR,Waring MJ

    更新日期:1993-03-16 00:00:00

  • Exposure of galactosylceramide to galactose oxidase in liposomes: dependence on lipid environment and ceramide composition.

    abstract::Factors which influence the accessibility, or exposure, of the carbohydrate head group of the glycolipid galactosylceramide (GalCer) at the membrane surface have been examined in lipid model membranes using the technique of galactose oxidase-tritiated sodium borohydride labeling. Both the ceramide composition of GalCe...


    pub_type: 杂志文章


    authors: Stewart RJ,Boggs JM

    更新日期:1993-06-01 00:00:00

  • Mechanism of binding of the inhibitor (E)-3-(furan-2-yl)-N-hydroxyacrylamide to a histone deacetylase-like amidohydrolase.

    abstract::Histone deacetylases have proven to be attractive novel targets for the treatment of cancer. The first inhibitor of histone deacetylases was approved for the treatment of cutaneous T-cell lymphoma in 2006. The identification of new lead structures with improved effectiveness and fewer side effects is necessary. This r...


    pub_type: 杂志文章


    authors: Sykora J,Meyer-Almes FJ

    更新日期:2010-02-23 00:00:00

  • A new omega-conotoxin that targets N-type voltage-sensitive calcium channels with unusual specificity.

    abstract::A new specific voltage-sensitive calcium channel (VSCC) blocker has been isolated from the venom of the fish-hunting cone snail Conus consors. This peptide, named omega-Ctx CNVIIA, consists of 27 amino acid residues folded by 3 disulfide bridges. Interestingly, loop 4, which is supposed to be crucial for selectivity, ...


    pub_type: 杂志文章


    authors: Favreau P,Gilles N,Lamthanh H,Bournaud R,Shimahara T,Bouet F,Laboute P,Letourneux Y,Ménez A,Molgó J,Le Gall F

    更新日期:2001-12-04 00:00:00

  • The photosystem of Rhodobacter sphaeroides assembles with zinc bacteriochlorophyll in a bchD (magnesium chelatase) mutant.

    abstract::A Rhodobacter sphaeroides bchD (magnesium chelatase) mutant was studied to determine the properties of its photosystem in the absence of bacteriochlorophyll (BChl). Western blots of reaction center H, M, and L (RC H/M/L) proteins from mutant membranes showed levels of 12% RC H, 32% RC L, and 46% RC M relative to those...


    pub_type: 杂志文章


    authors: Jaschke PR,Beatty JT

    更新日期:2007-10-30 00:00:00

  • Protein kinase C acylation by palmitoyl coenzyme A facilitates its translocation to membranes.

    abstract::Protein kinase C (PKC) translocation to specific subcellular membrane loci after cellular stimulation is mediated, in part, through its interaction with diacylglycerol, phosphatidylserine, and calcium. Herein, we present multiple lines of evidence which demonstrate that purified rabbit brain PKC undergoes specific acy...


    pub_type: 杂志文章


    authors: Ford DA,Horner CC,Gross RW

    更新日期:1998-08-25 00:00:00

  • Processes of base-pair opening and proton exchange in Z-DNA.

    abstract::Using proton magnetic resonance, we have investigated imino and amino proton exchange in the Z form of the four oligomers d(Cbr8GCGCbr8G), d(CGm5CGCG), d(CG)6, and d(CG)12. In the latter two oligomers, all five exchangeable protons have been assigned. We find that proton acceptors such as NH3 or the basic form of Tris...


    pub_type: 杂志文章


    authors: Kochoyan M,Leroy JL,Guéron M

    更新日期:1990-05-22 00:00:00

  • Systematic evaluation of candidate ligands regulating ectodomain shedding of amyloid precursor protein.

    abstract::Despite intense interest in the proteolysis of the β-Amyloid Precursor Protein (APP) in Alzheimer's disease, how the normal processing of this type I receptor-like glycoprotein is physiologically regulated remains ill-defined. In recent years, several candidate protein ligands for APP, including F-spondin, Reelin, β1 ...


    pub_type: 杂志文章


    authors: Rice HC,Young-Pearse TL,Selkoe DJ

    更新日期:2013-05-14 00:00:00

  • Complete structure of the adhesin receptor polysaccharide of Streptococcus oralis ATCC 55229 (Streptococcus sanguis H1).

    abstract::This report describes the determination of the complete primary structure of the adhesin receptor polysaccharide of Streptococcus oralis ATCC 55229 (previously characterized as Streptococcus sanguis H1), a Gram-positive bacteria implicated in dental plaque formation. The polysaccharide was isolated from S. oralis ATCC...


    pub_type: 杂志文章


    authors: Glushka J,Cassels FJ,Carlson RW,van Halbeek H

    更新日期:1992-11-10 00:00:00

  • Assembly of A beta amyloid protofibrils: an in vitro model for a possible early event in Alzheimer's disease.

    abstract::Amyloid fibrils comprising primarily the peptides A beta 40 and A beta 42 are a defining feature of the Alzheimer's disease (AD) brain, and convergent evidence suggests that the process of their formation plays a central role in the AD pathogenic pathway. Elucidation of fibril assembly is critical for the discovery of...


    pub_type: 杂志文章


    authors: Harper JD,Wong SS,Lieber CM,Lansbury PT Jr

    更新日期:1999-07-13 00:00:00

  • Discovery and characterization of cooperative ligand binding in the adaptive region of interleukin-2.

    abstract::The cytokine hormone interleukin-2 (IL-2) contains a highly adaptive region that binds small, druglike molecules. The binding properties of this adaptive region have been explored using a "tethering" method that relies on the formation of a disulfide bond between the protein and small-molecule ligands. Using tethering...


    pub_type: 杂志文章


    authors: Hyde J,Braisted AC,Randal M,Arkin MR

    更新日期:2003-06-03 00:00:00

  • Deuterium kinetic isotope effects and the mechanism of the bacterial luciferase reaction.

    abstract::A combined experimental and theoretical investigation of the deuterium isotope effects on the bacterial luciferase reaction is described. The experimental studies focus on determining if the unusual aldehydic deuterium isotope effect of approximately 1.5 observed in these reactions is an intrinsic isotope effect resul...


    pub_type: 杂志文章


    authors: Francisco WA,Abu-Soud HM,DelMonte AJ,Singleton DA,Baldwin TO,Raushel FM

    更新日期:1998-02-24 00:00:00