Sequence specificity of internal methylation in B77 avian sarcoma virus RNA subunits.

abstract：:The transglutaminase Factor XIII (FXIII) catalyzes the formation of covalent cross-links between adjacent noncovalently associated fibrin chains in blood coagulation. The resulting covalently cross-linked hard clot is much more mechanically stable and resistant to proteolytic degradation. FXIII is activated by the ser...

journal_title：Biochemistry

authors： Turner BT Jr,Sabo TM,Wilding D,Maurer MC

更新日期：2004-08-03 00:00:00

abstract：:Anticoagulation factor II (ACF II) isolated from the venom of Agkistrodon acutus is an activated coagulation factor X-binding protein in a Ca(2+)-dependent fashion with marked anticoagulant activity. The equilibrium unfolding/refolding of apo-ACF II, holo-ACF II, and Tb(3+)-reconstituted ACF II in guanidine hydrochlor...

journal_title：Biochemistry

authors： Xu X,Liu Q,Xie Y

更新日期：2002-03-19 00:00:00

abstract：:We have studied the kinetics of transfer of glucocerebroside between phospholipid bilayers by using pyrene and 3H-labeled glucocerebroside incorporated into dimyristoylphosphatidylcholine (DMPC) and dipalmitoylphosphatidylcholine (DPPC) bilayers. Pyrene-labeled glucocerebroside (PyrCer) molecules are able to form an e...

journal_title：Biochemistry

authors： Correa-Freire MC,Barenholz Y,Thompson TE

更新日期：1982-03-16 00:00:00

abstract：:In our previous work [Yang, Z. W., & Babitch, J. A. (1988) Biochemistry (preceding paper in this issue)] divalent cations were found to be more effective promoters of astroglial filament formation than were monovalent cations. To determine if one or more divalent cation binding sites were the basis for this difference...

journal_title：Biochemistry

authors： Yang ZW,Kong CF,Babitch JA

更新日期：1988-09-06 00:00:00

abstract：:Lysine side chains induce conformational changes in plasminogen (Pg) that regulate the process of fibrinolysis or blood clot dissolution. A lysine side-chain mimic, epsilon amino caproic acid (EACA), enhances the activation of Pg by urinary-type and tissue-type Pg activators but inhibits Pg activation induced by strep...

journal_title：Biochemistry

authors： Lin LF,Houng A,Reed GL

更新日期：2000-04-25 00:00:00

abstract：:A variant of the Tetrahymena ribozyme that efficiently cleaves single-stranded DNA under simulated physiological conditions [Tsang, J., & Joyce, G. F. (1994) Biochemistry 33, 5966-5973] was evaluated as a potential therapeutic agent on the basis of its ability to cleave synthetic oligonucleotide substrates correspondi...

journal_title：Biochemistry

authors： Raillard SA,Joyce GF

更新日期：1996-09-10 00:00:00

abstract：:Alzheimer's disease is a debilitating neurodegenerative disorder whose pathology has been linked to the aggregation and deposition of the amyloid β-peptide (Aβ) in neural tissue. A truly effective therapeutic agent remains elusive, and attention has recently turned to the use of natural products as effective antiaggre...

journal_title：Biochemistry

authors： Lemkul JA,Bevan DR

更新日期：2012-07-31 00:00:00

abstract：:G protein-coupled receptor (GPCR) kinases (GRKs) instigate the desensitization of activated GPCRs via phosphorylation that promotes interaction with arrestins, thereby preventing the interaction of GPCRs with heterotrimeric G proteins. A current proposed model of GRK1 activation involves the binding of activated rhodo...

journal_title：Biochemistry

authors： Orban T,Huang CC,Homan KT,Jastrzebska B,Tesmer JJ,Palczewski K

更新日期：2012-04-24 00:00:00

abstract：:Some ascidians sequester vanadium and other metal ions that are bound and transported in higher organisms by transferrin. The ascidian Ciona intestinalis has a monolobal transferrin (nicatransferrin) in its plasma. The binding of vanadium(V) to nicatransferrin was investigated by using isothermal titration calorimetry...

journal_title：Biochemistry

authors： Gaffney JP,Valentine AM

更新日期：2009-12-15 00:00:00

abstract：:Serine transacetylase from Salmonella typhimurium was purified to near homogeneity. A complete initial velocity study in both reaction directions suggests that the enzyme catalyzes the conversion of acetyl CoA and L-serine to O-acetyl-L-serine (OAS) and coenzyme A (CoASH) by a ping pong bi bi kinetic mechanism. Initia...

journal_title：Biochemistry

authors： Leu LS,Cook PF

更新日期：1994-03-08 00:00:00

abstract：:Alanine scanning mutagenesis, double mutant cycles, and X-ray crystallography were used to characterize the interface between the anti-hen egg white lysozyme (HEL) antibody D1.3 and HEL. Twelve out of the 13 nonglycine contact residues on HEL, as determined by the high-resolution crystal structure of the D1.3-HEL comp...

journal_title：Biochemistry

authors： Dall'Acqua W,Goldman ER,Lin W,Teng C,Tsuchiya D,Li H,Ysern X,Braden BC,Li Y,Smith-Gill SJ,Mariuzza RA

更新日期：1998-06-02 00:00:00

abstract：:The noncatalytic beta-subunit of protein kinase CK2 has been shown to display various and in some respects antagonistic effects on the catalytic alpha-subunit [Boldyreff et al. (1993) Biochemistry 32, 12672-12677; Meggio et al. (1994) Biochemistry 33, 4336-4342]. We have now examined the ability of peptides encompassi...

journal_title：Biochemistry

authors： Marin O,Meggio F,Sarno S,Pinna LA

更新日期：1997-06-10 00:00:00

abstract：:The head of the P22 bacteriophage is interrupted by a unique dodecameric portal vertex that serves as a conduit for the entrance and exit of the DNA. Here, the in vitro unfolding/refolding processes of the portal protein of P22 were investigated at different temperatures (1, 25, and 37 degrees C) through the use of ur...

journal_title：Biochemistry

authors： Braga CA,Carvalho D,Lara FA,Cortines JR,Moore SD,Prevelige PE Jr,Foguel D

更新日期：2007-06-26 00:00:00

abstract：:The proton-translocating NADH-quinone oxidoreductase (NDH-1) of Paracoccus denitrificans is composed of 14 different subunits (Nqo1-Nqo14). Of these, seven subunits (Nqo7, Nqo8, and Nqo10-14) which are equivalent to the mitochondrial DNA-encoded subunits of complex I constitute the membrane segment of the enzyme compl...

journal_title：Biochemistry

authors： Kao MC,Di Bernardo S,Matsuno-Yagi A,Yagi T

更新日期：2003-04-22 00:00:00

abstract：:The discovery of three mutants in the -synuclein (alphaSyn) gene and the identification of alphaSyn as the major component of Lewy body have opened a new field for understanding the pathogenesis of Parkinson's disease (PD). AlphaSyn is a natively unfolded protein with unknown function and unspecified conformational he...

journal_title：Biochemistry

authors： Jiang C,Chang JY

更新日期：2007-01-16 00:00:00

abstract：:Cofilin is an actin regulatory protein that binds to both monomeric and filamentous actin, and has filament severing activity. Although crystal structures for the monomeric forms of both G-actin and cofilin have been described, the structure of the binary cofilin-G-actin complex is not available. Synchrotron protein f...

journal_title：Biochemistry

authors： Guan JQ,Vorobiev S,Almo SC,Chance MR

更新日期：2002-05-07 00:00:00

abstract：:Picosecond absorption spectroscopy has been used to investigate energy-transfer dynamics within the LH1 and LH2 light-harvesting complexes of three mutants of Rhodobacter sphaeroides. We demonstrate that both complexes are inhomogeneous; each contains a specialized pigment pool which absorbs maximally at a longer wave...

journal_title：Biochemistry

authors： Hunter CN,Bergström H,van Grondelle R,Sundström V

更新日期：1990-04-03 00:00:00

abstract：:Novel inhibitors of lupin diadenosine 5',5'''-P(1),P(4)-tetraphosphate (Ap(4)A) hydrolase have been identified by in silico screening of a large virtual chemical library. Compounds were ranked on the basis of a consensus from six scoring functions. From the top 100 ranked compounds six were selected and initially scre...

journal_title：Biochemistry

authors： Branson KM,Mertens HD,Swarbrick JD,Fletcher JI,Kedzierski L,Gayler KR,Gooley PR,Smith BJ

更新日期：2009-08-18 00:00:00

abstract：:To study the role of the N-terminal region of the estrogen receptor (ER) in transcription activation and in DNA binding, we constructed a mutant of the Xenopus laevis ER which lacks amino acids 1-159 (XER160/586). In transient transfections, XER160/586 exhibited < 10% of the activity of wild-type XER on a synthetic pr...

journal_title：Biochemistry

authors： Xing H,Mattick S,Lew D,Shapiro DJ

更新日期：1995-03-28 00:00:00

abstract：:Recently, we revealed the architecture of the clamp-loader-helicase (tau-DnaB) complex in Bacillus by atomic force microscopy imaging and constructed a structural model, whereby a pentameric clamp-loader interacts with the hexameric helicase. Crucial to this model is the assumption that the clamp-loader forms a pentam...

journal_title：Biochemistry

authors： Haroniti A,Till R,Smith MC,Soultanas P

更新日期：2003-09-23 00:00:00

abstract：:The Xenopus borealis somatic 5S ribosomal RNA gene was used as a model system to determine the mutual effects of nucleosome folding and formation of ultraviolet (UV) photoproducts (primarily cis-syn cyclobutane pyrimidine dimers, or CPDs) in chromatin. We analyzed the preferred rotational and translational settings of...

journal_title：Biochemistry

authors： Liu X,Mann DB,Suquet C,Springer DL,Smerdon MJ

更新日期：2000-01-25 00:00:00

abstract：:The periplasmic protein CusF, as a part of the CusCFBA efflux complex, plays a role in resistance to elevated levels of copper and silver in Escherichia coli. Although homologues have been identified in other Gram-negative bacteria, the substrate of CusF and its precise role in metal resistance have not been described...

journal_title：Biochemistry

authors： Kittleson JT,Loftin IR,Hausrath AC,Engelhardt KP,Rensing C,McEvoy MM

更新日期：2006-09-19 00:00:00

abstract：:Thymidylate synthase from phage T4 (T4TS) is part of a complex of several enzymes required for coordinate DNA synthesis in infected Escherichia coli cells. It has been proposed that similar complexes of enzymes related to DNA synthesis are also functional in eukaryotes [Pardee, A. B. (1989) Science 246, 603-608]. To d...

journal_title：Biochemistry

authors： Finer-Moore JS,Maley GF,Maley F,Montfort WR,Stroud RM

更新日期：1994-12-27 00:00:00

abstract：:Anatoxin-a and homoanatoxin-a are two potent cyanobacterial neurotoxins. We recently reported the identification of the gene cluster responsible for the biosynthesis of these toxins as well as the in-vitro reconstitution of the first steps of this biosynthesis. We now report experimental evidence supporting the propos...

journal_title：Biochemistry

authors： Mann S,Lombard B,Loew D,Méjean A,Ploux O

更新日期：2011-08-23 00:00:00

abstract：:During initiation of minus-strand synthesis by HIV-1 reverse transcriptase, a 3'-DNA-RNA-5' junction is formed involving the 3'-end of tRNAlys,3. The HIV-RT-associated RNase H cleaves the RNA template strand specifically, opposite the newly synthesized DNA strand. We have determined the crystal structure at 1.9 A reso...

journal_title：Biochemistry

authors： Mueller U,Maier G,Mochi Onori A,Cellai L,Heumann H,Heinemann U

更新日期：1998-09-01 00:00:00

abstract：:We have developed a homogeneous time-resolved fluorescence resonance energy transfer (FRET)-based assay that detects the formation of HIV-1 integrase (IN) dimers. The assay utilizes IN monomers that express two different epitope tags that are recognized by their respective antibodies, coupled to distinct fluorophores....

journal_title：Biochemistry

authors： Tsiang M,Jones GS,Hung M,Samuel D,Novikov N,Mukund S,Brendza KM,Niedziela-Majka A,Jin D,Liu X,Mitchell M,Sakowicz R,Geleziunas R

更新日期：2011-03-15 00:00:00

abstract：:We report here on the Raman spectra of NADH, 3-acetylpyridine adenine dinucleotide, APAD+, and a fragment of these molecules, adenosine 5'-diphosphate ribose (ADPR) bound to the mitochondrial (mMDH) and cytoplasmic (or soluble, sMDH) forms of malate dehydrogenase. We observe changes in the Raman spectrum of the adenos...

journal_title：Biochemistry

authors： Deng H,Burgner J,Callender R

更新日期：1991-09-10 00:00:00

abstract：:The rate of the reaction between p-nitroperoxybenzoic acid and cytochrome c peroxidase (CcP) has been investigated as a function of pH and ionic strength. The pH dependence of the reaction between CcP and peracetic acid has also been determined. The rate of the reactions are influenced by two heme-linked ionizations i...

journal_title：Biochemistry

authors： Palamakumbura AH,Foshay MC,Vitello LB,Erman JE

更新日期：1999-11-23 00:00:00

abstract：:During catalysis by liver alcohol dehydrogenase (ADH), a water bound to the catalytic zinc is replaced by the oxygen of the substrates. The mechanism might involve a pentacoordinated zinc or a double-displacement reaction with participation by a nearby glutamate residue, as suggested by studies of human ADH3, yeast AD...

journal_title：Biochemistry

authors： Plapp BV,Savarimuthu BR,Ferraro DJ,Rubach JK,Brown EN,Ramaswamy S

更新日期：2017-07-18 00:00:00

abstract：:Illuminating Ca2+-depleted photosystem (PS) II membranes generated two new EPR signals at g = 11 and 15 by perpendicular and parallel polarization modes, respectively. Two turnovers of the oxygen evolving center (OEC) beyond the modified S2' state are required for the appearance of these signals. The formation of the ...

journal_title：Biochemistry

authors： Mino H,Kawamori A,Matsukawa T,Ono T

更新日期：1998-03-03 00:00:00