Physical dissection of the structural elements responsible for regulatory properties and intersubunit interactions of protein kinase CK2 beta-subunit.


:The noncatalytic beta-subunit of protein kinase CK2 has been shown to display various and in some respects antagonistic effects on the catalytic alpha-subunit [Boldyreff et al. (1993) Biochemistry 32, 12672-12677; Meggio et al. (1994) Biochemistry 33, 4336-4342]. We have now examined the ability of peptides encompassing the N- and C-terminal regions of the beta-subunit (beta[1-77] and beta[155-215]) to mimic the functions of the whole-length beta-subunit. Peptide beta[155-215] possesses only the positive features of the beta-subunit in that it prevents thermal inactivation and stimulates basal activity of the alpha-subunit, while it does not inhibit but rather stimulates calmodulin phosphorylation. In sharp contrast, peptide beta[1-77] neither protects the alpha-subunit nor stimulates its basal activity, while acting as a powerful and specific inhibitor of calmodulin phosphorylation. Peptide beta[155-215], but not peptide beta[1-77], stably interacts with alpha-subunit and also displays remarkable self-associating properties. A shorter derivative of beta[155-215], beta[170-215], displaying weaker stimulatory properties fails to stably interact with the alpha-subunit and to give rise to dimeric/multimeric forms. These data show that the elements responsible for the negative regulation are concentrated in the N-terminal moiety of the beta-subunit, whereas the C-terminal region retains the beneficial properties of the beta-subunit and is capable of self-association and binding of the alpha-subunit. Residues between 155 and 170 are necessary for the latter functions.






Marin O,Meggio F,Sarno S,Pinna LA




Has Abstract


1997-06-10 00:00:00














  • Prion diseases and their biochemical mechanisms.

    abstract::Prion diseases, also known as the transmissible spongiform encephalopathies (TSEs), are a group of fatal neurodegenerative disorders that affect humans and animals. These diseases are intimately associated with conformational conversion of the cellular prion protein, PrP(C), into an oligomeric beta-sheet-rich form, Pr...


    pub_type: 杂志文章,评审


    authors: Cobb NJ,Surewicz WK

    更新日期:2009-03-31 00:00:00

  • NMR structure of the extended Myb cognate sequence and modeling studies on specific DNA-Myb complexes.

    abstract::The recognition sequence of the Myb protein has been recently described to be pyAACKGHH (where py = T/C, K = G/T, and H = A/C/T), modifying the earlier identification as pyAACKG [Ording, E., et al. (1994) Eur. J. Biochem. 222, 113-120]. We had earlier determined the solution structure of the minimal cognate sequence T...


    pub_type: 杂志文章


    authors: Radha PK,Patel PK,Hosur RV

    更新日期:1998-07-14 00:00:00

  • Biochemical and biological characterization of neutrophil chemotactic protein, a novel rabbit CXC chemokine from alveolar macrophages.

    abstract::The role of interleukin-8 (IL-8) and related CXC chemokines has been demonstrated in many human diseases. However, more profound studies, e.g., by blocking the effect of these inflammatory mediators, request animal models and hence the identification of all human counterparts for commonly used laboratory animals. In t...


    pub_type: 杂志文章


    authors: Wuyts A,Schutyser E,Menten P,Struyf S,D'Haese A,Bult H,Opdenakker G,Proost P,Van Damme J

    更新日期:2000-11-28 00:00:00

  • Restricted motion of the lipoyl-lysine swinging arm in the pyruvate dehydrogenase complex of Escherichia coli.

    abstract::The three lipoyl (E2plip) domains of the dihydrolipoyl acetyltransferase component of the pyruvate dehydrogenase (PDH) complex of Escherichia coli house the lipoyl-lysine side chain essential for active-site coupling and substrate channelling within the complex. The structure of the unlipoylated form of the innermost ...


    pub_type: 杂志文章


    authors: Jones DD,Stott KM,Howard MJ,Perham RN

    更新日期:2000-07-25 00:00:00

  • Cutinase from Fusarium solani pisi hydrolyzing triglyceride analogues. Effect of acyl chain length and position in the substrate molecule on activity and enantioselectivity.

    abstract::Triglyceride analogues were synthesized in which one of the primary acyl ester functions has been replaced by an alkyl group and the secondary acyl ester bond has been replaced by an acyl amino bond. The chain length at either position was varied, and both (R)- and (S)-enantiomers of each compound were synthesized. Th...


    pub_type: 杂志文章


    authors: Mannesse ML,Cox RC,Koops BC,Verheij HM,de Haas GH,Egmond MR,van der Hijden HT,de Vlieg J

    更新日期:1995-05-16 00:00:00

  • Structure of alpha-glycerophosphate oxidase from Streptococcus sp.: a template for the mitochondrial alpha-glycerophosphate dehydrogenase.

    abstract::The FAD-dependent alpha-glycerophosphate oxidase (GlpO) from Enterococcus casseliflavus and Streptococcus sp. was originally studied as a soluble flavoprotein oxidase; surprisingly, the GlpO sequence is 30-43% identical to those of the alpha-glycerophosphate dehydrogenases (GlpDs) from mitochondrial and bacterial sour...


    pub_type: 杂志文章


    authors: Colussi T,Parsonage D,Boles W,Matsuoka T,Mallett TC,Karplus PA,Claiborne A

    更新日期:2008-01-22 00:00:00

  • Ca(2+)-dependent binding of endonexin (annexin IV) to membranes: analysis of the effects of membrane lipid composition and development of a predictive model for the binding interaction.

    abstract::Endonexin (annexin IV) is a member of the annexin family of homologous proteins that bind membranes and aggregate vesicles in a calcium-dependent fashion. This study examines the lipid modulation and mechanism of the binding of endonexin to membranes using a fluorescence energy transfer assay to measure bovine endonex...


    pub_type: 杂志文章


    authors: Junker M,Creutz CE

    更新日期:1994-08-02 00:00:00

  • Cyclobutane-type pyrimidine photodimer formation and excision in human skin fibroblasts after irradiation with 313-nm ultraviolet light.

    abstract::The formation and excision of 313-nm light-induced cyclobutane-type pyrimidine photodimers were determined in confluent cultures of human fibroblasts. A new method was developed for the resolution and determination of cytosine-thymine (CT) and thymine-thymine dimers (TT) by using sodium borohydride reduction and high-...


    pub_type: 杂志文章


    authors: Niggli HJ,Cerutti PA

    更新日期:1983-03-15 00:00:00

  • Biosynthesis of prolyl hydroxylase: evidence for two separate dolichol-media pathways of glycosylation.

    abstract::Prolyl hydroxylase is a glycoprotein containing two nonidentical subunits, alpha and beta. The alpha subunit of prolyl hydroxylase isolated from 13-day-old chick embryos contains a single high mannose oligosaccharide having seven mannosyl residues. Two forms of alpha subunit have been shown to exist in enzyme purified...


    pub_type: 杂志文章


    authors: Kedersha NL,Tkacz JS,Berg RA

    更新日期:1985-10-08 00:00:00

  • Inhibition of InhA, the enoyl reductase from Mycobacterium tuberculosis, by triclosan and isoniazid.

    abstract::Structural and genetic studies indicate that the antibacterial compound triclosan, an additive in many personal care products, is an inhibitor of EnvM, the enoyl reductase from Escherichia coli. Here we show that triclosan specifically inhibits InhA, the enoyl reductase from Mycobacterium tuberculosis and a target for...


    pub_type: 杂志文章


    authors: Parikh SL,Xiao G,Tonge PJ

    更新日期:2000-07-04 00:00:00

  • Quaternary structure of the HSC70 cochaperone HIP.

    abstract::HSC70 interacting protein (HIP) is an essential cytoplasmic cochaperone involved in the regulation of HSC70 chaperone activity and the maturation of progesterone receptor. To determine the quaternary structure and the gross conformation of the protein in solution, a wide array of biochemical and biophysical techniques...


    pub_type: 杂志文章


    authors: Velten M,Villoutreix BO,Ladjimi MM

    更新日期:2000-01-18 00:00:00

  • In vitro activity differences between proteins of the ADF/cofilin family define two distinct subgroups.

    abstract::The actin depolymerizing factor (ADF)/cofilins are an essential group of proteins that are important regulators of actin filament turnover in vivo. Although protists and yeasts express only a single member of this family, metazoans express two or more members in many cell types. In cells expressing both ADF and cofili...


    pub_type: 杂志文章


    authors: Chen H,Bernstein BW,Sneider JM,Boyle JA,Minamide LS,Bamburg JR

    更新日期:2004-06-08 00:00:00

  • Fluorescence spectroscopic studies of pressure effects on Na+,K(+)-ATPase reconstituted into phospholipid bilayers and model raft mixtures.

    abstract::To contribute to the understanding of membrane protein function upon application of pressure as relevant for understanding, for example, the physiology of deep sea organisms or for baroenzymological biotechnical processes, we investigated the influence of hydrostatic pressure on the activity of Na+,K+-ATPase enriched ...


    pub_type: 杂志文章


    authors: Powalska E,Janosch S,Kinne-Saffran E,Kinne RK,Fontes CF,Mignaco JA,Winter R

    更新日期:2007-02-13 00:00:00

  • The malonyl/acetyltransferase and beta-ketoacyl synthase domains of the animal fatty acid synthase can cooperate with the acyl carrier protein domain of either subunit.

    abstract::The active form of the animal fatty acid synthase (FAS) is a dimer of identical multifunctional polypeptides, each containing seven discrete functional domains, that cooperate to form two centers for palmitate synthesis. To assess the importance of domain cooperation across the subunit interface in the reaction mechan...


    pub_type: 杂志文章


    authors: Joshi AK,Witkowski A,Smith S

    更新日期:1998-02-24 00:00:00

  • Transient aggregation and stable dimerization induced by introducing an Alzheimer sequence into a water-soluble protein.

    abstract::Transient contacts between denatured polypeptide chains are likely to play an important part in the initial stages of protein aggregation and fibrillation. To analyze the nature of such contacts, we have carried out a protein engineering study of the 102-residue protein U1A, which aggregates transiently in the wild-ty...


    pub_type: 杂志文章


    authors: Otzen DE,Miron S,Akke M,Oliveberg M

    更新日期:2004-10-19 00:00:00

  • A bifunctional fusion protein containing Fc-binding fragment B of staphylococcal protein A amino terminal to antidigoxin single-chain Fv.

    abstract::A bifunctional molecule was genetically engineered which contained an amino-terminal effector domain that bound immunoglobulin Fc (fragment B of staphylococcal protein A) and a carboxyl-terminal domain that bound digoxin [a single-chain Fv (sFv)]. Effector and sFv binding properties were virtually identical with those...


    pub_type: 杂志文章


    authors: Tai MS,Mudgett-Hunter M,Levinson D,Wu GM,Haber E,Oppermann H,Huston JS

    更新日期:1990-09-04 00:00:00

  • Lipid bilayer partitioning and stability of camptothecin drugs.

    abstract::The intense intrinsic fluorescence emissions from several clinically relevant camptothecin drugs have been exploited in order to determine (1) the structural basis of drug binding to lipid bilayers, (2) the lipid bilayer stability of each drug's alpha-hydroxylactone moiety, a pharmacophore which is essential for antit...


    pub_type: 杂志文章


    authors: Burke TG,Mishra AK,Wani MC,Wall ME

    更新日期:1993-05-25 00:00:00

  • Mechanism of inhibition of the (Ca2(+)-Mg2+)-ATPase by nonylphenol.

    abstract::The effects of nonylphenol and 3,5-dibutyl-4-hydroxytoluene (BHT) on the activity of the (Ca2(+)-Mg2+)-ATPase of skeletal muscle sarcoplasmic reticulum have been studied. At high concentrations, both inhibit the ATPase activity of the ATPase either in native lipid or in bilayers of dioleoylphosphatidylcholine but, at ...


    pub_type: 杂志文章


    authors: Michelangeli F,Orlowski S,Champeil P,East JM,Lee AG

    更新日期:1990-03-27 00:00:00

  • A transforming growth factor-alpha-Pseudomonas exotoxin hybrid protein undergoes pH-dependent conformational changes conducive to membrane interaction.

    abstract::TP40 is a chimeric protein containing transforming growth factor alpha (TGF-alpha) at the N-terminus and a derivative of a 40,000-Da segment (PE40 delta cys) of Pseudomonas exotoxin (PE). PE40 delta cys contains domains Ib, II, and III of PE in which the cysteines are mutated to alanines. The rationale for inclusion o...


    pub_type: 杂志文章


    authors: Sanyal G,Marquis-Omer D,Gress JO,Middaugh CR

    更新日期:1993-04-06 00:00:00

  • 1H NMR assignment and global fold of napin BnIb, a representative 2S albumin seed protein.

    abstract::Napin BnIb is a representative member of the 2S albumin seed proteins, which consists of two polypeptide chains of 3.8 and 8.4 kDa linked by two disulfide bridges. In this work, a complete assignment of the 1H spectra of napin BnIb has been carried out by two-dimensional NMR sequence-specific methods and its secondary...


    pub_type: 杂志文章


    authors: Rico M,Bruix M,González C,Monsalve RI,Rodríguez R

    更新日期:1996-12-10 00:00:00

  • Mapping the G-actin binding surface of cofilin using synchrotron protein footprinting.

    abstract::Cofilin is an actin regulatory protein that binds to both monomeric and filamentous actin, and has filament severing activity. Although crystal structures for the monomeric forms of both G-actin and cofilin have been described, the structure of the binary cofilin-G-actin complex is not available. Synchrotron protein f...


    pub_type: 杂志文章


    authors: Guan JQ,Vorobiev S,Almo SC,Chance MR

    更新日期:2002-05-07 00:00:00

  • Identification of the catalytic nucleophile of tRNA (m5U54)methyltransferase.

    abstract::A covalent complex between tRNA (m5U54)methyltransferase, 5-fluorouridine tRNA(Phe), and S-adenosyl-L-[methyl-3H]methionine was formed in vitro and purified. Previously, it was shown that in this complex the 6-position of fluorouridine-54 is covalently linked to a catalytic nucleophile and the 5-position is bound to t...


    pub_type: 杂志文章


    authors: Kealey JT,Santi DV

    更新日期:1991-10-08 00:00:00

  • A Rational Design Strategy for the Selective Activity Enhancement of a Molecular Chaperone toward a Target Substrate.

    abstract::Molecular chaperones facilitate the folding and assembly of proteins and inhibit their aberrant aggregation. They thus offer several opportunities for biomedical and biotechnological applications, as for example they can often prevent protein aggregation more effectively than other therapeutic molecules, including sma...


    pub_type: 杂志文章


    authors: Aprile FA,Sormanni P,Vendruscolo M

    更新日期:2015-08-18 00:00:00

  • Hypertriglyceridemic VLDL decreases plasminogen binding to endothelial cells and surface-localized fibrinolysis.

    abstract::The effect of normo (NTG)- and hypertriglyceridemic (HTG)-VLDL on cultured human umbilical vein endothelial cell (HUVEC) surface-localized fibrinolysis was examined following pre-incubation with NTG-, HTG-VLDL, LDL (1-20 micrograms/mL) or buffer (control). Ligand binding assays, using 125I-labeled tcu-PA, t-PA, or Glu...


    pub_type: 杂志文章


    authors: Li XN,Koons JC,Benza RL,Parks JM,Varma VK,Bradley WA,Gianturco SH,Taylor KB,Grammer JR,Tabengwa EM,Booyse FM

    更新日期:1996-05-14 00:00:00

  • Structure of the nucleoid-associated protein Cnu reveals common binding sites for H-NS in Cnu and Hha.

    abstract::Cnu is a nucleoid protein that has a high degree of sequence homology with Hha/YmoA family proteins, which bind to chromatin and regulate the expression of Escherichia coli virulence genes in response to changes in temperature or ionic strength. Here, we determined its solution structure and dynamic properties and map...


    pub_type: 杂志文章


    authors: Bae SH,Liu D,Lim HM,Lee Y,Choi BS

    更新日期:2008-02-19 00:00:00

  • Carboxylate ions are strong allosteric ligands for the HisB10 sites of the R-state insulin hexamer.

    abstract::The insulin hexamer is an allosteric protein which displays positive and negative cooperativity and half-site reactivity that is modulated by strong homotropic and heterotropic ligand binding interactions at two different loci. These loci consist of phenolic pockets situated on the dimer-dimer interfaces of T-R and R-...


    pub_type: 杂志文章


    authors: Huang ST,Choi WE,Bloom C,Leuenberger M,Dunn MF

    更新日期:1997-08-12 00:00:00

  • Effects of an anti-beta monoclonal antibody on the interaction of the Escherichia coli RNA polymerase with the lac and TAC promoters.

    abstract::The effects of an inhibitory monoclonal antibody (mAb) raised against the beta subunit of the Escherichia coli RNA polymerase were determined on the kinetics and structural interactions during formation of the open promoter complex (RPo). Analysis of the kinetics of abortive initiation on linear and supercoiled templa...


    pub_type: 杂志文章


    authors: Rockwell P,Krakow JS

    更新日期:1988-05-03 00:00:00

  • Remodeling hexose-1-phosphate uridylyltransferase: mechanism-inspired mutation into a new enzyme, UDP-hexose synthase.

    abstract::Hexose-1-phosphate uridylyltransferase catalyzes the interconversion of UDP-galactose and glucose-1-P with UDP-glucose and galactose-1-P by a double-displacement mechanism through a covalent intermediate (E-UMP), in which UMP is bonded to one of two histidine residues at the active site, H164 or H166. To identify whic...


    pub_type: 杂志文章


    authors: Kim J,Ruzicka F,Frey PA

    更新日期:1990-11-27 00:00:00

  • Implication of His68 in the substrate site of Bacillus subtilis adenylosuccinate lyase by mutagenesis and affinity labeling with 2-[(4-bromo-2,3-dioxobutyl)thio]adenosine 5'-monophosphate.

    abstract::Adenylosuccinate lyase of Bacillus subtilis is inactivated by 2-[(4-bromo-2,3-dioxobutyl)thio]adenosine 5'-monophosphate (2-BDB-TAMP) at pH 7.0. As the reagent concentration is increased, a maximum rate constant is approached, indicative of reversible enzyme-reagent complex formation (KR = 68 +/- 9 microM) prior to ir...


    pub_type: 杂志文章


    authors: Lee TT,Worby C,Bao ZQ,Dixon JE,Colman RF

    更新日期:1998-06-09 00:00:00

  • Movement of the Rieske iron-sulfur protein in the p-side bulk aqueous phase: effect of lumenal viscosity on redox reactions of the cytochrome b6f complex.

    abstract::Based on the atomic structures of the mitochondrial cytochrome bc(1) complex, it has been proposed that the soluble domain of the [2Fe-2S] Rieske iron-sulfur protein (ISP) must rotate by ca. 60 degrees and translate through an appreciable distance between two binding sites, proximal to cytochrome c(1) and to the lumen...


    pub_type: 杂志文章


    authors: Heimann S,Ponamarev MV,Cramer WA

    更新日期:2000-03-14 00:00:00