Abstract:
:To identify how many rhodopsin intermediates interact with retinal G-protein transducin, the photobleaching process of chicken rhodopsin has been investigated in the presence or absence of transducin by means of time-resolved low-temperature spectroscopy. Singular value decomposition (SVD) analysis of the spectral data showed that a new intermediate called meta Ib is present between formally identified metarhodopsin I (now referred to as meta Ia) and metarhodopsin II (meta II). Since the absorption maximum of meta Ib (460 nm) is similar to that of meta Ia (480 nm), but considerably different from that of meta II (380 nm), meta Ib should have a protonated retinylidene Schiff base as its chromophore. Whereas transducin showed no effect on the conversion process between lumirhodopsin (lumi) and meta Ia, it affected the process between meta Ia and meta Ib and that between meta Ib and meta II. These results suggest that at least two intermediates (meta Ib and meta II) interact with transducin. The addition of GTPgammaS had no effect on the meta Ib-transducin interaction, while it abolished the ability of transducin to interact with meta II. Thus, meta Ib only binds to transducin, while meta II catalyzes a GDP-GTP exchange in transducin. These results suggest that deprotonation of the Schiff base chromophore is not necessary for the binding to transducin, while changes in protein structure including Schiff base deprotonation are needed to induce the GDP-GTP exchange in transducin.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Tachibanaki S,Imai H,Mizukami T,Okada T,Imamoto Y,Matsuda T,Fukada Y,Terakita A,Shichida Ydoi
10.1021/bi970932osubject
Has Abstractpub_date
1997-11-18 00:00:00pages
14173-80issue
46eissn
0006-2960issn
1520-4995pii
bi970932ojournal_volume
36pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::A general protocol for preparation of oligonucleotides containing intrastrand cross-links between the exocyclic amino groups of adjacent deoxyguanosines has been developed. A series of 2, 3, and 4 methylene cross-links was incorporated site-specifically into an 11-mer (5'-GGCAGGTGGTG-3', cross-linked positions are und...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi010450j
更新日期:2002-03-05 00:00:00
abstract::Sensory rhodopsin II (SRII) is unique among the archaeal rhodopsins in having an absorption maximum near 500 nm, blue shifted roughly 70 nm from the other pigments. In addition, SRII displays vibronic structure in the lambda(max) absorption band, whereas the other pigments display fully broadened band maxima. The mole...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0116487
更新日期:2001-11-20 00:00:00
abstract::Solutions of porcine lactate dehydrogenase of micromolar concentration kept at 4 degrees C for several days lose the greater part of their enzymic activity but recover it when returned to room temperature. The rate of spoiling decreases and the rate of recovery increases with the concentration of the solutions. The de...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00360a024
更新日期:1986-06-17 00:00:00
abstract::Polyclonal antibody to mitochondrial P-450c27/25 reacted with two proteins of apparent molecular masses of 52 kilodaltons (kDa) and 50 kDa from the female rat liver mitochondrial proteins bound to an omega-octylaminoagarose column. The two proteins were purified to greater than 85% homogeneity by DEAE-Sephacel and hyd...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00098a007
更新日期:1991-08-27 00:00:00
abstract::A PS I membrane preparation from a PS II deficient mutant of Synechocystis sp. PCC 6803 (psb DI/DII/C) was investigated by picosecond photovoltage and fluorescence measurements. The photovoltage kinetics show two distinct phases. At low excitation energies the fast phase correlates with the fluorescence decay time con...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00195a001
更新日期:1994-07-26 00:00:00
abstract::Cryptophycin-52 (LY355703) is currently undergoing clinical evaluation for cancer chemotherapy. It is a potent suppressor of microtubule dynamics in vitro, and low picomolar concentrations appear to inhibit cancer cell proliferation at mitosis by stabilizing spindle microtubules. In the present study, using [(3)H]cryp...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0010827
更新日期:2000-11-21 00:00:00
abstract::Internal fatty acylation of proteins is a recognized means of modifying biological behavior. Escherichia coli hemolysin A (HlyA), a toxic protein, is transcribed as a nontoxic protein and made toxic by internal acylation of two lysine residue epsilon-amino groups; HlyC catalyzes the acyl transfer from acyl-acyl carrie...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990138y
更新日期:1999-07-06 00:00:00
abstract::The structure of [14-38]Abu, a variant of bovine pancreatic trypsin inhibitor (BPTI) with only the 14-38 disulfide bridge intact, has been analyzed by two-dimensional 1H and 1H-15N NMR. Except for the 18-24, 29-35 antiparallel beta-sheet, residues in all regions of the molecule give two exchange cross peaks for each 1...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00036a015
更新日期:1995-09-12 00:00:00
abstract::A heterologous expression system was used to evaluate activation of BlaR1, a sensor/signal transducer protein of Staphylococcus aureus with a central role in resistance to β-lactam antibiotics. In the absence of other S. aureus proteins that might respond to antibiotics and participate in signal transduction events, w...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300429p
更新日期:2012-06-12 00:00:00
abstract::We have analyzed by band-shift assays HeLa cell protein-DNA interactions on a stable cruciform DNA molecule. The stable cruciform was formed by heteroduplexing the HindIII-SphI fragment of SV40 virus DNA that contains the origin of replication with a derivative mutant containing a heterologous substitution at the cent...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00251a030
更新日期:1994-11-29 00:00:00
abstract::Isolation and characterization of a novel radiation-induced product, i.e., the 8-hydroxyguanine residue, produced in deoxyribonucleic acid (DNA), 2'-deoxyguanosine, and 2'-deoxyguanosine 5'-monophosphate by gamma-irradiation in aqueous solution, are described. For this purpose, gamma-irradiated DNA was first hydrolyze...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00337a032
更新日期:1985-07-30 00:00:00
abstract::Several bifunctional alkylating agents of the aziridinylbenzoquinone class have been evaluated as potential antitumor agents. 3,6-Bis[(2-hydroxyethyl)amino]-2,5- diaziridinyl-1,4-benzoquinone (BZQ), 2,5-diaziridinyl-1,4-benzoquinone (DZQ), 3,6-bis(carboxyamino)-2,5-diaziridinyl- 1,4-benzoquinone (AZQ), and six analogu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00114a016
更新日期:1991-12-17 00:00:00
abstract::The Rieske protein from Thermus thermophilus (TtRp) and a truncated version of the protein (truncTtRp), produced to achieve a low-pH crystallization condition, have been characterized using UV-visible and circular dichroism spectroscopies. TtRp and truncTtRp undergo a change in the UV-visible spectra with increasing p...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi901126u
更新日期:2009-10-20 00:00:00
abstract::Factor IX activation by factor XIa is thought to proceed through the singly-cleaved free intermediate, factor IX alpha. However, we observed no intermediate development during factor IX activation by factor XIa when using a low substrate to enzyme ratio (44:1 mol/mol). This result can be explained by one of two mechan...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi962274y
更新日期:1997-04-08 00:00:00
abstract::We measure the effects of low concentrations of helix-stabilizing cosolvents, including 2,2,2-trifluoroethanol (TFE), on the thermodynamics and kinetics of folding of the dimeric alpha-helical coiled coil derived from the leucine zipper region of bZIP transcriptional activator GCN4. The change in kinetic behavior upon...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981641y
更新日期:1998-10-13 00:00:00
abstract::Carbohydrate-binding module (CBM) family 13 includes the "R-type" or "ricin superfamily" beta-trefoil lectins. The C-terminal CBM, CBM13, of xylanase 10A from Streptomyces lividans is a family 13 CBM that is not only structurally similar to the "R-type" lectins but also somewhat functionally similar. The primary funct...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi015865j
更新日期:2002-04-02 00:00:00
abstract::We have used a fluorescent analogue of ATP, mantATP [2'(3')-O-(N-methylanthraniloyl)-adenosine 5'-triphosphate; Hiratsuka T. (1983) Biochim. Biophys. Acta 742, 496-508], and made a detailed kinetic study of the interaction of mantATP and mantADP with S1 and acto-S1. We have shown that these analogues behave like ATP a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00216a017
更新日期:1991-01-15 00:00:00
abstract::Single-molecule imaging of the entrance of a protein into the hydrophobic environment of a cell membrane was investigated. The pre-stem of LukF, one of the two components of the pore-forming toxin staphylococcal gamma-hemolysin, was specifically labeled with 6-bromoacetyl-2-dimethylaminonaphthalene (Badan), an environ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0514156
更新日期:2006-02-28 00:00:00
abstract::Mutation of polycystin-1 (PC1) is the major cause of autosomal dominant polycystic kidney disease. PC1 has a predicted molecular mass of ~460 kDa comprising a long multidomain extracellular N-terminal region, 11 transmembrane regions, and a short C-terminal region. Because of its size, PC1 has proven difficult to hand...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300134b
更新日期:2012-04-03 00:00:00
abstract::The cystic fibrosis transmembrane conductance regulator forms an anion-selective channel. We previously showed that charge selectivity, the ability to discriminate between anions and cations, occurs near the cytoplasmic end of the channel. The molecular determinants of charge selectivity, however, are unknown. We inve...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990155n
更新日期:1999-04-27 00:00:00
abstract::Neuraminidase is one of the two glycoprotein spikes protruding from the influenza virus membrane. We have determined by X-ray crystallography the native structure of B/Lee/40 neuraminidase (NA) and the structures of its crystals soaked with a substrate, N-acetylneuraminyllactose (NANL), and an inhibitor, 2-deoxy-2,3-d...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00193a002
更新日期:1994-07-12 00:00:00
abstract::Two-dimensional homonuclear and heteronuclear NMR and minimized potential energy calculations have been combined to define the structure of the antitumor agent mitomycin C (MC) cross-linked to deoxyguanosines on adjacent base pairs in the d(T1-A2-C3-G4-T5-A6).d(T7-A8-C9-G10-T11-A12) duplex. The majority of the mitomyc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00463a032
更新日期:1990-03-20 00:00:00
abstract::The hydrogen exchange kinetics of the complex of trypsin-soybean trypsin inhibitor (Kunitz) have been compared to the calculated sum of the exchange kinetics for the inhibitor and trypsin measured separately. The exchange rates observed for the complex are substantially less than the sum of the exchange rates in the t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00686a020
更新日期:1975-07-29 00:00:00
abstract::The repeating units of mouse, rat, and alpha-monkey satellites have been cloned. All three show properties that are characteristic of curved DNA: (i) their migration in polyacrylamide gels is slower than predicted from their sequences, and (ii) they appear as curved molecules when visualized by electron microscopy. Al...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00461a019
更新日期:1990-03-06 00:00:00
abstract::In the anaerobic sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough (DvH), the genome sequencing revealed the presence of three operons encoding formate dehydrogenases. fdh1 encodes an alphabetagamma trimeric enzyme containing 11 heme binding sites; fdh2 corresponds to an alphabetagamma trimeric enzyme wi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0515366
更新日期:2005-11-15 00:00:00
abstract::Nuclear magnetic resonance (NMR) has been used to monitor the conformation and dynamics of the d(C1-G2-A3-T4-T5-A6-T6-A5-A4-T3-C2-G1) self-complementary dodecanucleotide duplex (henceforth called Pribnow 12-mer), which contains a TATAAT Pribnow box and a central core of eight dA X dT base pairs. The exchangeable imino...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00325a019
更新日期:1985-02-12 00:00:00
abstract::Homeodomain-only protein (HOP) is an 8-kDa transcriptional corepressor that is essential for the normal development of the mammalian heart. Previous studies have shown that HOP, which consists entirely of a putative homeodomain, acts downstream of Nkx2.5 and associates with the serum response factor (SRF), repressing ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi060641s
更新日期:2006-09-05 00:00:00
abstract::While the critical role of the bacterial luciferase alpha subunit in catalysis has been amply documented, the beta subunit was only known to be involved in thermal stability and substrate binding. Two conserved histidyl residues at position 81 and 82 of the beta subunit of Vibrio harveyi luciferase were each mutated t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00206a023
更新日期:1994-10-11 00:00:00
abstract::Polyalanine (polyA) is the third-most prevalent homopeptide repeat in eukaryotes, behind polyglutamine and polyasparagine. Abnormal expansion of the polyA repeat is linked to at least nine human diseases, and the disease mechanism likely involves enhanced length-dependent aggregation. Because of the simplicity of its ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi201155g
更新日期:2011-11-01 00:00:00
abstract::In iron-deficient conditions, Pseudomonas aeruginosa secretes a major fluorescent siderophore named pyoverdin (Pvd), which after chelating iron(III) is transported back into the cell via its outer membrane receptor FpvA. FpvA is a TonB-dependent transport protein and has the ability to bind Pvd in its apo- or iron-loa...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049768c
更新日期:2004-06-22 00:00:00