Abstract:
:A heterologous expression system was used to evaluate activation of BlaR1, a sensor/signal transducer protein of Staphylococcus aureus with a central role in resistance to β-lactam antibiotics. In the absence of other S. aureus proteins that might respond to antibiotics and participate in signal transduction events, we documented that BlaR1 fragmentation is autolytic, that it occurs in the absence of antibiotics, and that BlaR1 directly degrades BlaI, the gene repressor of the system. Furthermore, we disclosed that this proteolytic activity is metal ion-dependent and that it is not modulated directly by acylation of the sensor domain by β-lactam antibiotics.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Llarrull LI,Mobashery Sdoi
10.1021/bi300429psubject
Has Abstractpub_date
2012-06-12 00:00:00pages
4642-9issue
23eissn
0006-2960issn
1520-4995journal_volume
51pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Multiple forms of the acute-phase serum protein SAA were isolated from the lipoprotein fraction of plasma from a single individual. These protein forms were purified by size-exclusion, ion-exchange, and reverse-phase high-pressure liquid chromatography, and then the tryptic peptides were subjected to amino acid sequen...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00405a044
更新日期:1988-03-08 00:00:00
abstract::The substrate specificity of the beta-glucosidase (CelB) from the hyperthermophilic archaeon Pyrococcus furiosus, a family 1 glycosyl hydrolase, has been studied at a molecular level. Following crystallization and X-ray diffraction of this enzyme, a 3.3 A resolution structural model has been obtained by molecular repl...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi992463r
更新日期:2000-05-02 00:00:00
abstract::A cDNA coding for a P450 expressed in human lung was isolated from a lambda gt11 library constructed from human lung mRNA using a cDNA probe to rat P450 IVA1. The cDNA-deduced amino acid sequence of this P450, designated IVB1, consisted of 511 amino acids and had a calculated molecular weight of 59,558. The IVB1 amino...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00446a014
更新日期:1989-10-03 00:00:00
abstract::B₁₂-dependent enzymes employ radical species with exceptional prowess to catalyze some of the most chemically challenging, thermodynamically unfavorable reactions. However, dealing with highly reactive intermediates is an extremely demanding task, requiring sophisticated control strategies to prevent unwanted side rea...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500050k
更新日期:2014-06-17 00:00:00
abstract::The thermodynamics of L-tryptophan and operator DNA binding to the tryptophan repressor of Escherichia coli were analyzed by titration microcalorimetry and van't Hoff analysis of footprinting titrations, respectively. At 25 degrees C in 10 mM sodium phosphate, pH 7.6, and 0.1 M NaCl, the binding of L-tryptophan to the...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00079a029
更新日期:1993-07-20 00:00:00
abstract::The solution structure of the dicerium(III) complex of the N-terminal domain of calmodulin (Ce2-TR1C hereafter) has been solved employing paramagnetic T1 relaxation enhancements and pseudocontact shifts introduced by the Ce3+ ions, together with conventional NOE constraints. The use of pseudocontact shift constraints ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi971022+
更新日期:1997-09-30 00:00:00
abstract::Photolyases reverse the effects of UV light on cells by converting cyclobutane dipyrimidine photoproducts (pyrimidine dimers, Pyr mean value of Pyr) into pyrimidine monomers in a light-dependent reaction. Previous work has suggested that, based on substrate preference, there are two classes of photolyase: DNA photolya...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00099a019
更新日期:1991-09-03 00:00:00
abstract::Site-directed mutants were prepared of four consecutive and highly conserved residues (His-411, Asp-412, Thr-413, Tyr-414) of an extramembrane loop that connects putative transmembrane helices IX and X of subunit I of Rhodobacter sphaeroides cytochrome c oxidase. The modified enzymes were purified and analyzed by opti...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00171a019
更新日期:1994-02-08 00:00:00
abstract::The binding of agonists, antagonists, and the acetylcholinesterase inhibitor, eserine, to the nicotinic acetylcholine receptor from Torpedo californica has been monitored by the fluorescence changes of two extrinsic probes that have been covalently attached to the receptor protein. Although both probes, 5-(iodoacetami...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00084a031
更新日期:1993-08-24 00:00:00
abstract::The structure of the copper site in oxidized and reduced Rhus vernicifera stellacyanin has been studied by X-ray absorption (XAFS) spectroscopy at different pH values. Data for the oxidized protein are consistent with the fourth ligand being an O- or N-donating ligand rather than a cysteine from the disulfide bridge. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00001a026
更新日期:1995-01-10 00:00:00
abstract::The ligand binding relationship between the acetylcholine transporter (AcChT) and the vesamicol receptor (VR) and the kinetics of active transport were studied in synaptic vesicles purified from the Torpedo electric organ using analogues of AcCh and vesamicol. Methoxyvesamicol, which should exhibit better equilibratio...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00140a010
更新日期:1992-06-30 00:00:00
abstract::We have examined the C cluster in type II CO dehydrogenase (CODH) from Carboxydothermus hydrogenformans using Ni K-edge X-ray absorption near edge spectroscopy and extended X-ray absorption fine structure (EXAFS) spectroscopy. The enzyme was studied under three conditions: "as-isolated" and after treatment with CO or ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi036104e
更新日期:2004-07-20 00:00:00
abstract::Ryanodine receptor (RyR) mutations linked with some congenital skeletal and cardiac diseases are localized to three easily definable regions: region 1 (N-terminal domain), region 2 (central domain), and a rather broad region 3 containing the channel pore. As shown in our recent studies, the interdomain interaction bet...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061557f
更新日期:2007-04-10 00:00:00
abstract::Pseudomonas aeruginosa PA4872 was identified by sequence analysis as a structurally and functionally novel member of the PEP mutase/isocitrate lyase superfamily and therefore targeted for investigation. Substrate screens ruled out overlap with known catalytic functions of superfamily members. The crystal structure of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi701954p
更新日期:2008-01-08 00:00:00
abstract::The photocycle of the blue-light photoreceptor protein Photoactive Yellow Protein (PYP) was studied at reduced relative humidity (RH). Photocycle kinetics and spectra were measured in thin films of PYP in which the relative humidity was set at values between 29 and 98% RH with saturated solutions of various salts. We ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi050237d
更新日期:2005-06-28 00:00:00
abstract::The complex of dioldehydrase with adenosylcobalamin (coenzyme B12) and potassium ion reacts with molecular oxygen in the absence of a substrate to oxidize coenzyme and inactivate the complex. In this article, high performance liquid chromatography and mass spectral analysis are used to identify the nucleoside products...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi700078z
更新日期:2007-06-19 00:00:00
abstract::An issue that is central to understanding cellular fatty acid (FA) metabolism is whether physiologic transport of FA across cell membranes requires protein mediation or can be satisfied by the rate of spontaneous movement through the lipid phase. For this reason, considerable effort has been devoted to determining the...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi962007s
更新日期:1997-05-13 00:00:00
abstract::The members of the ets gene family of transcription factors are characterized by a conserved 85-residue DNA-binding region, termed the ETS domain, that lacks sequence homology to structurally characterized DNA-binding motifs. The secondary structure of the ETS domain of murine Ets-1 was determined on the basis of NMR ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00250a001
更新日期:1994-11-22 00:00:00
abstract::The high resolution proton nuclear magnetic resonance (NMR) spectra of the synthetic DNA block polymer d(C15A15)-d(T15G15) were studied in order to more completely understand telestability in DNA, and to provide fundamental NMR data on DNA helices and random coils. Spectra were measured in the spectral region from 0 t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00622a031
更新日期:1977-02-08 00:00:00
abstract::We have studied the pathway and kinetics of refolding of recombinant human brain-derived neurotrophic factor (BDNF), which is a very tightly-associated dimer in its native state. When BDNF unfolded in 6 M guanidine hydrochloride is diluted 20-fold into phosphate-buffered saline, a partially folded intermediate is rapi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00091a036
更新日期:1993-10-12 00:00:00
abstract::Triple helix-forming oligonucleotides (TFOs) bind with high affinity and specificity to homopurine-homopyrimidine sequences in DNA and have been shown to inhibit transcription of target genes in various experimental systems. In the present study, we evaluated the ability of 3'-amino-modified phosphodiester TFOs direct...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi992185w
更新日期:2000-05-02 00:00:00
abstract::High signal-to-noise Fourier transform infrared (FTIR) spectra of the 5-hydroxytryptamine (serotonin) receptor (5-HT(3)R) and the nicotinic acetylcholine receptor (nAChR) were obtained by microscope FTIR spectroscopy using micrometer-sized, fully hydrated protein films. Because this novel procedure requires only nanog...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035113k
更新日期:2003-12-02 00:00:00
abstract::HbPresbyterian (beta 108Asn --> Lys, HbP) contains an additional positive charge (per alpha beta dimer) in the middle of the central cavity and exhibits a lower oxygen affinity than wild-type HbA in the presence of chloride. However, very little is known about the molecular origins of its altered functional properties...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9827464
更新日期:1999-08-31 00:00:00
abstract::Human erythrocyte spectrin prepared from fresh blood is a mixture of different association states. Depending on the manner of preparation, the two-chain dimer or the tetramer predominates. These forms are not in rapid thermodynamic equilibrium. The molecular weight of the dimer by sedimentation and diffusion and by li...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00644a028
更新日期:1977-12-13 00:00:00
abstract::The X-ray crystal structures of Escherichia coli gamma-aminobutyrate aminotransferase unbound and bound to the inhibitor aminooxyacetate are reported. The enzyme crystallizes from ammonium sulfate solutions in the P3(2)21 space group with a tetramer in the asymmetric unit. Diffraction data were collected to 2.4 A reso...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049218e
更新日期:2004-08-31 00:00:00
abstract::The structure of empty capsids of Kilham rat virus, an autonomous parvovirus with icosahedral symmetry, was investigated by small-angle neutron scattering. From the forward scatter, the molecular weight was determined to be 4.0 X 10(6), and from the Guinier region, the radius of gyration was found to be 105 A in D2O a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00321a044
更新日期:1984-12-18 00:00:00
abstract::CO dehydrogenase from Clostridium thermoaceticum is a nickel-containing enzyme that catalyzes both the reversible conversion of CO2 to CO (for incorporation into the carbonyl group of acetate) and the synthesis of acetyl-CoA from methyl corrinoid, CO, and CoASH. The latter activity is conveniently assayed by monitorin...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00420a019
更新日期:1988-10-04 00:00:00
abstract::The structure of a synthetic peptide corresponding to residues 1-45 of rabbit muscle adenylate kinase has been studied in aqueous solution by two-dimensional NMR, FTIR, and CD spectroscopy. This peptide, which binds MgATP and is believed to represent most of the MgATP-binding site of the enzyme [Fry, D.C., Kuby, S.A.,...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00410a009
更新日期:1988-05-17 00:00:00
abstract::Phytochromes are widespread red/far-red photosensory proteins well known as critical regulators of photomorphogenesis in plants. It is often assumed that natural selection would have optimized the light sensing efficiency of phytochromes to minimize nonproductive photochemical deexcitation pathways. Surprisingly, the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500108s
更新日期:2014-05-06 00:00:00
abstract::The aminoacylation kinetics of 19 different variants of yeast tRNATyr with nucleotide substitutions in positions 33-35 were determined. Substitution of the conserved uridine-33 does not alter the rate of aminoacylation. However, substitution of the anticodon position 34 or position 35 reduces Km from 2- to 10-fold and...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00367a072
更新日期:1986-09-23 00:00:00