当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Factor IX activation by factor XIa proceeds without release of a free intermediate.

Factor IX activation by factor XIa proceeds without release of a free intermediate.

Abstract：

:Factor IX activation by factor XIa is thought to proceed through the singly-cleaved free intermediate, factor IX alpha. However, we observed no intermediate development during factor IX activation by factor XIa when using a low substrate to enzyme ratio (44:1 mol/mol). This result can be explained by one of two mechanisms: (1) factor XIa-catalyzed activation proceeds via a singly-cleaved free intermediate with a much higher efficiency of cleavage than factor IX zymogen, or (2) the reaction occurs without free intermediate generation, whereby factor XIa makes both proteolytic cleavages in a single substrate molecule before releasing the final product (processive mechanism). We compared the factor XIa cleavage rates of free factor IX alpha and factor IXa alpha with that of factor IX zymogen. In contrast to the requirements of mechanism (1), the cleavage rate constants of factor IX zymogen, factor IX alpha, and factor IXa alpha were similar: 0.38 +/- 0.02 s(-1), 0.34 +/- 0.05 s(-1), and 0.27 +/- 0.01 s(-1), respectively. It seems likely that factor XIa-generated intermediates observed under some reaction conditions are produced through the occasional failure of a processive mechanism. Indeed, in reactions using a high substrate to enzyme ratio (1900:1 mol/mol), we observed some factor IX alpha development; however, the pattern of intermediate and product development over time was inconsistent with a mechanism involving an obligate intermediate. Rather, it corresponded to behavior expected from a processive mechanism undergoing a consistent low failure. We conclude that factor XIa-catalyzed activation of factor IX proceeds via a processive mechanism without release of a free intermediate.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Wolberg AS,Morris DP,Stafford DW

doi

10.1021/bi962274y

subject

Has Abstract

pub_date

1997-04-08 00:00:00

pages

4074-9

issue

14

eissn

0006-2960

issn

1520-4995

pii

bi962274y

journal_volume

36

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Recombinant human CNTF receptor alpha: production, binding stoichiometry, and characterization of its activity as a diffusible factor.

    abstract：:The primary ligand-binding protein (CNTFR alpha) of the multicomponent receptor for ciliary neurotrophic factor was produced in Escherichia coli. Using novel applications of size-exclusion chromatography and a protein gel-shift assay, we obtained quantitative separation of correctly refolded protein, as well as analyt...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00185a020

    authors： Panayotatos N,Everdeen D,Liten A,Somogyi R,Acheson A

    更新日期：1994-05-17 00:00:00

  • Identification of the six ligands to manganese(II) in transition-state-analogue complexes of creatine kinase: oxygen-17 superhyperfine coupling from selectively labeled ligands.

    abstract：:The complete coordination scheme for Mn(II) in transition-state-analogue complexes with creatine kinase has been determined by electron paramagnetic resonance (EPR) spectroscopy. Perturbations in the EPR spectra for Mn(II) due to superhyperfine coupling to 17O of selectively labeled ligands have been used to identify ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00565a002

    authors： Reed GH,Leyh TS

    更新日期：1980-11-25 00:00:00

  • Tubulin-G protein association stabilizes GTP binding and activates GTPase: cytoskeletal participation in neuronal signal transduction.

    abstract：:It has been suggested that dimeric tubulin can participate in the signal transduction process through its association with the GTP-binding (G) proteins Gs and Gi1. Using the photoaffinity GTP analog, azidoanilido-GTP, it has been shown that the transfer of nucleotide from tubulin to G alpha s and G alpha i1 is the key...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00198a052

    authors： Roychowdhury S,Rasenick MM

    更新日期：1994-08-16 00:00:00

  • An FTIR investigation of flanking sequence effects on the structure and flexibility of DNA binding sites.

    abstract：:Fourier transform infrared (FTIR) spectroscopy and a library of FTIR marker bands have been used to examine the structure and relative flexibilities conferred by different flanking sequences on the EcoRI binding site. This approach allowed us to examine unique peaks and subtle changes in the spectra of d(AAAGAATTCTTT)...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi8015235

    authors： Kahn TR,Fong KK,Jordan B,Lek JC,Levitan R,Mitchell PS,Wood C,Hatcher ME

    更新日期：2009-02-17 00:00:00

  • Development of a streak-camera-based time-resolved microscope fluorimeter and its application to studies of membrane fusion in single cells.

    abstract：:A time-resolved microscope fluorimeter based on a synchroscan streak camera and a fast pulsed laser system has been developed to measure the fluorescence lifetime decay under the fluorescence microscope. This system allows one to measure the nanosecond fluorescence lifetimes of fluorophores in a small spot (0.8-6.3 mi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00240a024

    authors： Kusumi A,Tsuji A,Murata M,Sako Y,Yoshizawa AC,Kagiwada S,Hayakawa T,Ohnishi S

    更新日期：1991-07-02 00:00:00

  • Reevaluation of the stoichiometry of cytochrome b559 in photosystem II and thylakoid membranes.

    abstract：:The stoichiometry of cytochrome b559 (one or two copies) per reaction center of photosystem II (PSII) has been the subject of considerable debate. The molar ratio of cytochrome b559 has a number of significant implications on our understanding of the functional role of cytochrome b559, the mechanism of electron donati...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00161a024

    authors： Buser CA,Diner BA,Brudvig GW

    更新日期：1992-11-24 00:00:00

  • Evolutionary optimization of the catalytic effectiveness of an enzyme.

    abstract：:The kinetic and thermodynamic features of reactions catalyzed by present-day enzymes appear to be the consequence of the evolution of these proteins toward maximal catalytic effectiveness. These features are identified and analyzed (in detail for one substrate-one product enzymes) by using ideas that link the energeti...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00450a009

    authors： Burbaum JJ,Raines RT,Albery WJ,Knowles JR

    更新日期：1989-11-28 00:00:00

  • Redox regulation of epidermal growth factor receptor signaling through cysteine oxidation.

    abstract：:Epidermal growth factor receptor (EGFR) exemplifies the family of receptor tyrosine kinases that mediate numerous cellular processes, including growth, proliferation, and differentiation. Moreover, gene amplification and EGFR mutations have been identified in a number of human malignancies, making this receptor an imp...

    journal_title：Biochemistry

    pub_type： 杂志文章,评审

    doi：10.1021/bi301441e

    authors： Truong TH,Carroll KS

    更新日期：2012-12-18 00:00:00

  • Generation of a free alpha-amino group by Raney nickel after 2-nitro-5-thiocyanobenzoic acid cleavage at cysteine residues: application to automated sequencing.

    abstract：:The selective reaction of SH containing proteins and peptides with NTCB (2-nitro-5-thiocyanobenzoic acid) has been reported (Degani, Y., & Patchornick, A. (1974) Biochemistry 13, 1; Jacobson, G.A., Schaffer, M.H., Stark, G.R., & Vanaman, T.C. (1973) J. Biol. Chem. 248, 6583). With this reagent, cysteinyl peptide bonds...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00618a022

    authors： Otieno S

    更新日期：1978-12-12 00:00:00

  • Kinetic mechanism of myosinV-S1 using a new fluorescent ATP analogue.

    abstract：:We have used a new fluorescent ATP analogue, 3'-(7-diethylaminocoumarin-3-carbonylamino)-3'-deoxyadenosine-5'-triphosphate (deac-aminoATP), to study the ATP hydrolysis mechanism of the single headed myosinV-S1. Our study demonstrates that deac-aminoATP is an excellent substrate for these studies. Although the deac-ami...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi060712n

    authors： Forgacs E,Cartwright S,Kovács M,Sakamoto T,Sellers JR,Corrie JE,Webb MR,White HD

    更新日期：2006-10-31 00:00:00

  • Transient spectroscopy of bacterial rhodopsins with an optical multichannel analyzer. 2. Effects of anions on the halorhodopsin photocycle.

    abstract：:We find that the photocycle of halorhodopsin (HR) in the presence of nitrate (but not chloride) consists of two parallel series of reactions. The first is essentially the same as that which occurs in the presence of chloride: HRhv----HRK----HRKL----HRL----HRO----HR. The second photocycle, however, which we describe as...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00438a039

    authors： Zimányi L,Lanyi JK

    更新日期：1989-06-13 00:00:00

  • Conformations of primary amphipathic carrier peptides in membrane mimicking environments.

    abstract：:Two peptides designed for drug delivery were generated by the combination of a signal peptide with a nuclear localization sequence and are shown to facilitate the cellular internalization of small molecules which are covalently linked to these peptides. In order to understand the mechanism of internalization, the conf...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9708491

    authors： Chaloin L,Vidal P,Heitz A,Van Mau N,Méry J,Divita G,Heitz F

    更新日期：1997-09-16 00:00:00

  • Understanding the changes in the circular dichroism of light harvesting complex II upon varying its pigment composition and organization.

    abstract：:In this work we modeled the circular dichroism (CD) spectrum of LHCII, the main light harvesting antenna of photosystem II of higher plants. Excitonic calculations are performed for a monomeric subunit, taken from the crystal structure of trimeric LHCII from spinach [Liu, Z. F., Yan, H. C., Wang, K. B., Kuang, T. Y., ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi062031y

    authors： Georgakopoulou S,van der Zwan G,Bassi R,van Grondelle R,van Amerongen H,Croce R

    更新日期：2007-04-24 00:00:00

  • Distal pocket polarity in ligand binding to myoglobin: structural and functional characterization of a threonine68(E11) mutant.

    abstract：:Site-directed mutagenesis studies have confirmed that the distal histidine in myoglobin stabilizes bound O2 by hydrogen bonding and have suggested that it is the polar character of the imidazole side chain rather than its size that limits the rate of ligand entry into the protein. We constructed an isosteric Val68 to ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00239a025

    authors： Smerdon SJ,Dodson GG,Wilkinson AJ,Gibson QH,Blackmore RS

    更新日期：1991-06-25 00:00:00

  • Influence of ligation state and concentration of hemoglobin A on its cross-linking by glycolaldehyde: functional properties of cross-linked, carboxymethylated hemoglobin.

    abstract：:The ligation state of hemoglobin during its cross-linking by glycolaldehyde influences the ultimate oxygen affinity of the cross-linked protein. Thus, if the cross-linking is performed with carbonmonoxy-hemoglobin, the oxygen affinity increases slightly to a P50 of 7 mmHg from a P50 of 9 mmHg for unmodified hemoglobin...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00417a064

    authors： Manning LR,Manning JM

    更新日期：1988-08-23 00:00:00

  • The limits of promiscuity: isoform-specific dimerization of filamins.

    abstract：:Filamins are a family of actin cross-linking proteins that are primarily localized in the cortical cytoplasm of all mammalian cells. Until now, three major isoforms (filamins a, b, and c) have been identified, that were shown to be differentially expressed and/or localized in different tissues. An amino-terminal doubl...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi026501+

    authors： Himmel M,Van Der Ven PF,Stöcklein W,Fürst DO

    更新日期：2003-01-21 00:00:00

  • Solution structure of the 45-residue MgATP-binding peptide of adenylate kinase as examined by 2-D NMR, FTIR, and CD spectroscopy.

    abstract：:The structure of a synthetic peptide corresponding to residues 1-45 of rabbit muscle adenylate kinase has been studied in aqueous solution by two-dimensional NMR, FTIR, and CD spectroscopy. This peptide, which binds MgATP and is believed to represent most of the MgATP-binding site of the enzyme [Fry, D.C., Kuby, S.A.,...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00410a009

    authors： Fry DC,Byler DM,Susi H,Brown EM,Kuby SA,Mildvan AS

    更新日期：1988-05-17 00:00:00

  • Activation of epidermal growth factor receptor by epidermal growth factor.

    abstract：:The binding of epidermal growth factor (EGF) to epidermal growth factor receptor (EGF receptor) induces dimerization of the receptor and activation of its protein tyrosine kinase. Each of these three steps was followed as a function of the concentrations of EGF and of EGF receptor. Binding of EGF was followed by sedim...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9602268

    authors： Sherrill JM,Kyte J

    更新日期：1996-05-07 00:00:00

  • Enzyme kinetics in solvents of increased viscosity. Dynamic aspects of carbonic anhydrase catalysis.

    abstract：:The dependence of enzymatic catalysis on diffusion rates in solution was examined with regard to high specific activity carbonic anhydrase (CA II) by varying the viscosity of the reaction medium with added glycerol, sucrose, and ficoll (a copolymer of sucrose and epichlorohydrin). Responses of the Michaelis-Menten par...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00383a028

    authors： Pocker Y,Janjić N

    更新日期：1987-05-05 00:00:00

  • Site-directed mutagenesis, kinetic, and spectroscopic studies of the P-loop residues in a low molecular weight protein tyrosine phosphatase.

    abstract：:The structure of the specific phosphate binding loop (P-loop) of bovine protein tyrosine phosphatase (BPTP) is very similar to that present in high M(r) PTPases. Site-directed mutagenesis was used to explore the role of several conserved residues involved in forming the P-loop of BPTP. Thus, Ser-19 and Ser-43 were ind...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9605651

    authors： Evans B,Tishmack PA,Pokalsky C,Zhang M,Van Etten RL

    更新日期：1996-10-22 00:00:00

  • Cytochrome P450 from Photobacterium profundum SS9, a piezophilic bacterium, exhibits a tightened control of water access to the active site.

    abstract：:We report cloning, expression in Escherichia coli, and purification of cytochrome P450 from a deep-sea bacterium Photobacterium profundum strain SS9 (P450-SS9). The enzyme, which is predominately high spin (86%) in the absence of any added ligand, binds fatty acids and their derivatives and exhibits the highest affini...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi101466y

    authors： Sineva EV,Davydov DR

    更新日期：2010-12-21 00:00:00

  • Structure elucidation and preliminary assessment of hydrolase activity of PqsE, the Pseudomonas quinolone signal (PQS) response protein.

    abstract：:In bacteria, the transcription of virulence genes is usually controlled by a cell density-dependent process known as "quorum sensing" (QS). QS relies on small diffusible signaling molecules that cross the bacterial cell wall and activate target transcription factors after a threshold concentration has been reached. Be...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi900123j

    authors： Yu S,Jensen V,Seeliger J,Feldmann I,Weber S,Schleicher E,Häussler S,Blankenfeldt W

    更新日期：2009-11-03 00:00:00

  • Fluorescent probes as a measure of conformational alterations induced by nucleophilic modification and proteolysis of bovine alpha 2-macroglobulin.

    abstract：:Conformational alterations occurring in bovine alpha 2-macroglobulin (alpha 2M) resulting from proteolysis and nucleophilic modification have been monitored by UV difference spectra, circular dichroism, and changes in the fluorescence of 6-(p-toluidino)-2-naphthalenesulfonate (TNS) and bis(8-anilino-1-naphthalenesulfo...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00321a061

    authors： Strickland DK,Steiner JP,Feldman SR,Pizzo SV

    更新日期：1984-12-18 00:00:00

  • RNase T1 variant RV cleaves single-stranded RNA after purines due to specific recognition by the Asn46 side chain amide.

    abstract：:Attempts to alter the guanine specificity of ribonuclease T1 (RNase T1) by rational or random mutagenesis have failed so far. The RNase T1 variant RV (Lys41Glu, Tyr42Phe, Asn43Arg, Tyr45Trp, and Glu46Asn) designed by combination of a random and a rational mutagenesis approach, however, exhibits a stronger preference t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi035961f

    authors： Czaja R,Struhalla M,Höschler K,Saenger W,Sträter N,Hahn U

    更新日期：2004-03-16 00:00:00

  • Short-chain phosphatidylethanolamines: physical properties and susceptibility of the monomers to phospholipase A2 action.

    abstract：:The homologous series of optically active short-chain phosphatidylethanolamines (PE) from dibutyryl-PE to dioctanoyl-PE was synthesized. In addition, two monomeric short-chain phospholipid analogues that are not degraded by phospholipase A2 (1,2-bis[(butylcarbamyl)oxy]-sn-glycero-3-phosphocholine and the corresponding...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00336a058

    authors： Plückthun A,Rohlfs R,Davidson FF,Dennis EA

    更新日期：1985-07-16 00:00:00

  • Homogeneity and variability in the structure of azurin molecules studied by fluorescence decay and circular polarization.

    abstract：:The fluorescence decay of apoazurin derived from Pseudomonas aeruginosa is monoexponential. By this criterion the population of molecules of apoazurin is homogeneous. The emission anisotropy factor and the absorption anisotropy factor at the red edge of the absorption band assume similar values, showing that the trypt...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00680a018

    authors： Grinvald A,Schlessinger J,Pecht I,Steinberg IZ

    更新日期：1975-05-06 00:00:00

  • Crystal structures of two potent nonamidine inhibitors bound to factor Xa.

    abstract：:There has been intense interest in the development of factor Xa inhibitors for the treatment of thrombotic diseases. Our laboratory has developed a series of novel non-amidine inhibitors of factor Xa. This paper presents two crystal structures of compounds from this series bound to factor Xa. The first structure is de...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0264061

    authors： Adler M,Kochanny MJ,Ye B,Rumennik G,Light DR,Biancalana S,Whitlow M

    更新日期：2002-12-31 00:00:00

  • Phosphorus-31 nuclear magnetic resonance studies of the binding of nucleotides to NADP+-specific isocitrate dehydrogenase.

    abstract：:The interaction of the 2'-phosphate-containing nucleotides (NADP+, NADPH, 2'-phosphoadenosine 5'-diphosphoribose, and adenosine 2',5'-bisphosphate) with NADP+ -specific isocitrate dehydrogenase was studied by using 31P NMR spectroscopy. The separate resonances corresponding to free and bound nucleotides, characteristi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00303a015

    authors： Mas MT,Colman RF

    更新日期：1984-04-10 00:00:00

  • Effect of phosphorylation on hydrogen-bonding interactions of the active site histidine of the phosphocarrier protein HPr of the phosphoenolpyruvate-dependent phosphotransferase system determined by 15N NMR spectroscopy.

    abstract：:The phosphocarrier protein HPr of the phosphoenolpyruvate-dependent sugar transport system of Escherichia coli can exist in a phosphorylated and a nonphosphorylated form. During phosphorylation, the phosphoryl group is carried on a histidine residue, His15. The hydrogen-bonding state of this histidine was examined wit...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00487a026

    authors： van Dijk AA,de Lange LC,Bachovchin WW,Robillard GT

    更新日期：1990-09-04 00:00:00

  • Distinct reactions catalyzed by bacterial and yeast trans-aconitate methyltransferases.

    abstract：:The trans-aconitate methyltransferase from the bacterium Escherichia coli catalyzes the monomethyl esterification of trans-aconitate and related compounds. Using two-dimensional (1)H/(13)C nuclear magnetic resonance spectroscopy, we show that the methylation is specific to one of the three carboxyl groups and further ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0022902

    authors： Cai H,Strouse J,Dumlao D,Jung ME,Clarke S

    更新日期：2001-02-20 00:00:00