Distinct reactions catalyzed by bacterial and yeast trans-aconitate methyltransferases.

abstract：:Furanacryloyl-Phe-Gly-Gly has been shown to be a convenient substrate for angiotensin converting enzyme (dipeptidyl carboxypeptidase, EC 3.4.15.1). A detailed kinetic analysis of the hydrolysis of this substrate indicates normal Michaelis-Menten behavior with kcat = 19000 min-1 and KM = 3.0 x 10(-4) M determined at pH...

journal_title：Biochemistry

authors： Bünning P,Holmquist B,Riordan JF

更新日期：1983-01-04 00:00:00

abstract：:The crystallographic structure of oxygenated trHbN from Mycobacterium tuberculosis showed an extended heme distal site hydrogen-bonding network that includes Y(B10), Q(E11), and the bound O(2) (Milani, M., et al. (2001) EMBO J. 20, 3902-3909). In the present work, we analyze the effects that substitutions at the B10 a...

journal_title：Biochemistry

authors： Ouellet Y,Milani M,Couture M,Bolognesi M,Guertin M

更新日期：2006-07-25 00:00:00

abstract：:The effect of the polyene antibiotic amphotericin B on the electron paramagnetic resonance spectra of lipid probes intercalated in model membranes was examined. When the antibiotic was added to the aqueous phase, no spectral effects occurred. However, when the antibiotic was incorporated during membrane preparation, c...

journal_title：Biochemistry

authors： Aracava Y,Smith IC,Schreier S

更新日期：1981-09-29 00:00:00

abstract：:The chemical mechanism of the phosphoribosyltransferases (PRTases), although largely unknown, may proceed either via a concerted direct-transfer mechanism or with a two-step mechanism involving a carboxonium-like intermediate. To study this question, we have cloned the Salmonella typhimurium pyrE gene, coding for the ...

journal_title：Biochemistry

authors： Bhatia MB,Vinitsky A,Grubmeyer C

更新日期：1990-11-20 00:00:00

abstract：:SCG10 (superior cervical ganglia neural-specific 10 protein) is a neuron specific member of the stathmin family of microtubule regulatory proteins that like stathmin can bind to soluble tubulin and depolymerize microtubules. The direct actions of SCG10 on microtubules themselves and on their dynamics have not been inv...

journal_title：Biochemistry

authors： Manna T,Grenningloh G,Miller HP,Wilson L

更新日期：2007-03-20 00:00:00

abstract：:Numerous studies have been published stressing the importance of finding ligands that can bind specifically to DNA secondary structures. Several have identified ligands that are presented as having specific binding to the G-quadruplex; however, these were not originally tested on the complementary i-motif structure. T...

journal_title：Biochemistry

authors： Abdelhamid MAS,Gates AJ,Waller ZAE

更新日期：2019-01-29 00:00:00

abstract：:The Salmonella typhimurium leu-500 promoter is active only in topA strains. In an earlier study (Chen, D., Bowater, R., Dorman, C., & Lilley, D. M. J. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 8784-8788), we showed that the activity of this promoter on a circular plasmid is a function of the transcription and translati...

journal_title：Biochemistry

authors： Chen D,Bowater RP,Lilley DM

更新日期：1993-12-07 00:00:00

abstract：:The membrane-bound closed channel structure of helices 4 and 5 (Lys-406-Asp-446) of the colicin E1 channel domain was investigated by using a site-directed fluorescence labeling technique. A bimane probe was covalently attached to a cysteine residue in a series of single-cysteine mutant proteins to scan each helix in ...

journal_title：Biochemistry

authors： Ho D,Merrill AR

更新日期：2009-02-17 00:00:00

abstract：:A large set of glycome information was obtained from egg white proteins of 88 samples from Galloanserae (63 Anseriformes and 25 Galliformes). The data were obtained on whole N-glycan structures and types of sialic acids of these egg whites by glycoblotting-based high-throughput and quantitative glycomics. The results ...

journal_title：Biochemistry

authors： Hirose K,Amano M,Hashimoto R,Lee YC,Nishimura S

更新日期：2011-05-31 00:00:00

abstract：:In cells, RNAs likely adopt numerous intermediate conformations prior to formation of functional RNA-protein complexes. We used single-nucleotide resolution selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE) to probe the structure of Escherichia coli 16S rRNA in healthy growing bacteria. SHAPE-direct...

journal_title：Biochemistry

authors： McGinnis JL,Weeks KM

更新日期：2014-05-20 00:00:00

abstract：:Recent crystal structures of the small ribosomal subunit have made it possible to examine the detailed energetics of codon recognition on the ribosome by computational methods. The binding of cognate and near-cognate anticodon stem loops to the ribosome decoding center, with mRNA containing the Phe UUU and UUC codons,...

journal_title：Biochemistry

authors： Almlöf M,Andér M,Aqvist J

更新日期：2007-01-09 00:00:00

abstract：:The complete primary and three-dimensional solution structures of subtilosin A (1), a bacteriocin from Bacillus subtilis, were determined by multidimensional NMR studies on peptide produced using isotopically labeled [(13)C,(15)N]medium derived from Anabaena sp. grown on sodium [(13)C]bicarbonate and [(15)N]nitrate. A...

journal_title：Biochemistry

authors： Kawulka KE,Sprules T,Diaper CM,Whittal RM,McKay RT,Mercier P,Zuber P,Vederas JC

更新日期：2004-03-30 00:00:00

abstract：:Applications of sensitive new technologies, in particular, two-dimensional NMR spectroscopy, have allowed detection of folded structures in short peptide fragments of proteins in aqueous solution under conditions where native proteins fold. These structures are in rapid dynamic exchange with unfolded states. These obs...

journal_title：Biochemistry

authors： Wright PE,Dyson HJ,Lerner RA

更新日期：1988-09-20 00:00:00

abstract：:Transfection of cos-1 cells with either prostaglandin endoperoxide H synthase-1 (PGHS-1) or -2 (PGHS-2) results in a mixed population of cells containing a diverse range of expressed enzyme. The use of fluorescent substrates and antibodies, in conjunction with fluorescence microscopy, provides the means to quantitate ...

journal_title：Biochemistry

authors： Morita I,Smith WL,DeWitt DL,Schindler M

更新日期：1995-05-30 00:00:00

abstract：:The binding equilibrium of phospholipase A2 (PLA2) to the substrate interface influences many aspects of the overall kinetics of interfacial catalysis by this enzyme. For example, the interpretation of kinetic data on substrate specificity was difficult when there was a significant kinetic contribution from the interf...

journal_title：Biochemistry

authors： Ghomashchi F,Yu BZ,Berg O,Jain MK,Gelb MH

更新日期：1991-07-23 00:00:00

abstract：:Parkinson's disease (PD) involves loss of dopaminergic neurons in the substantia nigra and is characterized by intracellular inclusions, Lewy bodies, consisting primarily of aggregated alpha-synuclein. Two substitution mutations (A53T and A30P) in alpha-synuclein gene have been identified in familial early-onset PD. T...

journal_title：Biochemistry

authors： Moussa CE,Wersinger C,Tomita Y,Sidhu A

更新日期：2004-05-11 00:00:00

abstract：:The inhibitory potency of 3'-azido-3'-deoxythymidine 5'-triphosphate (AZTTP) against HIV-1 reverse transcriptase (HIV-1 RT) has been further evaluated. The results indicate that the previously reported low Ki values for AZTTP against HIV-1 RT (2.35 nM) are due neither to the to the direct tight binding of AZTTP to HIV...

journal_title：Biochemistry

authors： Ma QF,Bathurst IC,Barr PJ,Kenyon GL

更新日期：1992-02-11 00:00:00

abstract：:A preparation consisting of isolated dimeric peripheral antenna complexes from green plant photosystem I (light-harvesting complex I or LHCI) has been characterized by means of (polarized) steady-state absorption and fluorescence spectroscopy at low temperatures. We show that this preparation can be described reasonab...

journal_title：Biochemistry

authors： Ihalainen JA,Gobets B,Sznee K,Brazzoli M,Croce R,Bassi R,van Grondelle R,Korppi-Tommola JE,Dekker JP

更新日期：2000-07-25 00:00:00

abstract：:(Z)-3-(Fluoromethyl)phosphoenolpyruvate has been synthesized in nine chemical steps from glyoxylic acid. The compound is stable at pH 3, but at pH 8 it decomposes within seconds to give 2-oxo-3-butenoate. When 3-(fluoromethyl)phosphoenolpyruvate is added to a solution of phosphoenolpyruvate carboxylase or pyruvate kin...

journal_title：Biochemistry

authors： Wirsching P,O'Leary MH

更新日期：1988-02-23 00:00:00

abstract：:At pH 5.5, binding of bleomycin relaxed supercoiled ColE1 DNA without breaking it. Binding of tripeptide S, a fragment of the drug containing the bithiazole rings, also relaxed and then recoiled supercoiled DNA, at pH 5.5 and at pH 8.0, where bleomycin is normally active. The unwinding angle was 12 degrees. Both compo...

journal_title：Biochemistry

authors： Povirk LF,Hogan M,Dattagupta N

更新日期：1979-01-09 00:00:00

abstract：:Radical S-adenosyl-L-methionine, cobalamin-dependent methyltransferases have been proposed to catalyze the methylations of unreactive carbon or phosphorus atoms in antibiotic biosynthetic pathways. To date, none of these enzymes has been purified or shown to be active in vitro. Here we demonstrate the activity of the ...

journal_title：Biochemistry

authors： Werner WJ,Allen KD,Hu K,Helms GL,Chen BS,Wang SC

更新日期：2011-10-25 00:00:00

abstract：:We show here that expression of chromogranins in non-neuroendocrine NIH3T3 cells significantly increased the amount of IP(3)-mediated intracellular Ca(2+) mobilization in these cells, whereas suppression of them in neuroendocrine PC12 cells decreased the amount of mobilized Ca(2+). We have therefore investigated the r...

journal_title：Biochemistry

authors： Huh YH,Jeon SH,Yoo JA,Park SY,Yoo SH

更新日期：2005-04-26 00:00:00

abstract：:A strategy to design potent antagonists of human coagulation factor VIIa (FVIIa) by linking two proteins that independently inhibit activity and bind at separate, nonoverlapping sites is presented. A bifunctional inhibitor (KDTF5), comprising a Kunitz-type domain engineered to inhibit the FVIIa active site and a solub...

journal_title：Biochemistry

authors： Lee GF,Lazarus RA,Kelley RF

更新日期：1997-05-13 00:00:00

abstract：:The reaction center protein D1 in photosystem II shows a high turnover during illumination. The degradation of the D1 protein is preceded by photoinhibition of the electron transport in photosystem II. There are two distinct mechanisms for this: acceptor-side- and donor-side-induced photoinhibition. Here, donor-side-i...

journal_title：Biochemistry

authors： Jegerschöld C,Styring S

更新日期：1996-06-18 00:00:00

abstract：:Cations often deform the structure of regulatory proteins to affect a functional response, but for other protein functions a more passive effect is desired. For instance, it is shown here that in the conductance of Na+ by the gramicidin channel there appears to be no significant structural deformation of either the si...

journal_title：Biochemistry

authors： Tian F,Lee KC,Hu W,Cross TA

更新日期：1996-09-17 00:00:00

abstract：:MutY protein, a DNA glycosylase found in Escherichia coli, recognizes dA:dG, dA:8-oxodG, and dA:dC mismatches in duplex DNA, excising the adenine moiety. We have investigated the mechanism of action of MutY, addressing several points of disagreement raised by previous studies of this enzyme. MutY forms a covalent inte...

journal_title：Biochemistry

authors： Zharkov DO,Grollman AP

更新日期：1998-09-08 00:00:00

abstract：:Gluconacetobacter diazotrophicus stands out among the acetic acid bacteria as it fixes dinitrogen and is a true endophyte. It has a set of constitutive enzymes to oxidize ethanol and acetaldehyde which is upregulated during N(2)-dependent growth. The membrane-bound alcohol dehydrogenase (ADH) is a heterodimer (subunit...

journal_title：Biochemistry

authors： Gómez-Manzo S,Solano-Peralta A,Saucedo-Vázquez JP,Escamilla-Marván JE,Kroneck PM,Sosa-Torres ME

更新日期：2010-03-23 00:00:00

abstract：:Comparisons of catalytic mechanisms have not previously been performed for homologous enzymes from hyperthermophilic and mesophilic sources. Here, the beta-glucosidase from the hyperthermophilic archaeon Pyrococcus furiosus was recombinantly produced in Escherichia coli and shown to have biophyscial and biochemical pr...

journal_title：Biochemistry

authors： Bauer MW,Kelly RM

更新日期：1998-12-08 00:00:00

abstract：:The Caenorhabditis elegans Werner syndrome protein, WRN-1, a member of the RecQ helicase family, has a 3'-5' DNA helicase activity. Worms with defective wrn-1 exhibit premature aging phenotypes and an increased level of genome instability. In response to DNA damage, WRN-1 participates in the initial stages of checkpoi...

journal_title：Biochemistry

authors： Hyun M,Park S,Kim E,Kim DH,Lee SJ,Koo HS,Seo YS,Ahn B

更新日期：2012-02-21 00:00:00

abstract：:Some aminoacyl-tRNA synthetases (AARSs) employ an editing mechanism to ensure the fidelity of protein synthesis. Leucyl-tRNA synthetase (LeuRS), isoleucyl-tRNA synthetase (IleRS), and valyl-tRNA synthetase (ValRS) share a common insertion, called the CP1 domain, which is responsible for clearing misformed products. Th...

journal_title：Biochemistry

authors： Mascarenhas AP,Martinis SA

更新日期：2008-04-22 00:00:00