Abstract:
:The stoichiometry of cytochrome b559 (one or two copies) per reaction center of photosystem II (PSII) has been the subject of considerable debate. The molar ratio of cytochrome b559 has a number of significant implications on our understanding of the functional role of cytochrome b559, the mechanism of electron donation in PSII, and the stoichiometry of the other redox-active, reaction center components. We have reinvestigated the stoichiometry of cytochrome b559 in PSII-enriched and thylakoid membranes, using differential absorbance and electron paramagnetic resonance spectroscopies. The data from both quantitation procedures strongly indicate only one copy of cytochrome b559 per reaction center in PSII-enriched membranes and also suggest one copy of cytochrome b559 per reaction center in thylakoid membranes.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Buser CA,Diner BA,Brudvig GWdoi
10.1021/bi00161a024subject
Has Abstractpub_date
1992-11-24 00:00:00pages
11441-8issue
46eissn
0006-2960issn
1520-4995journal_volume
31pub_type
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