当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Left handed double helices: effect of sequence on the spatial configuration of high anti nucleic acids.

Left handed double helices: effect of sequence on the spatial configuration of high anti nucleic acids.

Abstract：

:The conformational properties of purine-pyrimidine and pyrimidine-purine dinucleoside monophosphates in which the glycosidic torsion is fixed to congruent to 120 degree by the formation of a covalent link between the base and the sugar ring are explored by 1H NMR spectroscopy in order to obtain information about the spatial configuration of high anti nucleic acids. The intramolecular stack of the high anti dimers were found to be left handed, in contrast to that (right handed) for natural oligomers, which are low anti. Even though both the high anti pyrimidine-purine and purine-pyrimidine dimers have similar backbone torsion angles, they display widely different relative geometry between the bases; thus in the former there is extensive base-base overlap in the stack, and in the latter there is negligible intramolecular base-base overlap. In addition it was found that purine-pyrimidine systems form miniature double helices in which there is substantial interstrand purine-purine interaction; on the other hand the pyridine-purine high anti dinucleosides have no proclivity to form such base-paired complexes in solution. Mathematical polymerization of the conformation of the high anti purine-pyrimidine dinucleoside monophosphates generates a left handed helix for high anti polynucleotides. This also means that the double helix for high anti-nucleic acids containing purine-pyrimidine repeated units may also be left handed, as had been suggested [Sundaralingam, M., & Yathindra, N. (1977) Int. J. Quantum Chem., Quantum Biol. Symp. 4, 285]. It is suggested that the plasticity in the structure of genomic DNA is such that, if under certain conditions of interactions the sugar-base torsion of certain domains assume high anti values, that domain will become left handed, and this in turn can be a mechanism for the control of expression by genomic DNA.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Dhingra MM,Sarma RH,Uesugi S,Shida T,Ikehara M

doi

10.1021/bi00520a029

subject

Has Abstract

pub_date

1981-08-18 00:00:00

pages

5002-11

issue

17

eissn

0006-2960

issn

1520-4995

journal_volume

20

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Structural similarities between 6-methylsalicylic acid synthase from Penicillium patulum and vertebrate type I fatty acid synthase: evidence from thiol modification studies.

    abstract：:6-Methylsalicylic acid synthase, the multifunctional enzyme complex that catalyzes the biosynthesis of the tetraketide 6-methylsalicylic acid, was modified by thiol-specific inhibitors and cross-linking reagents. Treatment with 1,3-dibromopropan-2-one caused rapid enzyme inactivation and formation of cross-linked dime...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi960422e

    authors： Child CJ,Spencer JB,Bhogal P,Shoolingin-Jordan PM

    更新日期：1996-09-24 00:00:00

  • Cryoelectron microscopy of Escherichia coli F1 adenosinetriphosphatase decorated with monoclonal antibodies to individual subunits of the complex.

    abstract：:Monoclonal antibodies directed against epitopes on each of the five subunits (alpha, beta, gamma, delta, and epsilon) of the Escherichia coli F1 ATPase (ECF1) have been prepared and used to localize the subunits in the enzyme complex. Fab' fragments, prepared by pepsin digestion of the antibodies, were bound to ECF1 a...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00437a031

    authors： Gogol EP,Aggeler R,Sagermann M,Capaldi RA

    更新日期：1989-05-30 00:00:00

  • Properties of a major protein released from Escherichia coli by osmotic shock.

    abstract：:A large fraction of a constitutively synthesized polypeptide, comprising 5% of the total Escherichia coli protein, is released when plasmolysed cells are subjected to osmotic shock into ice-cold water. Since the protein is not liberated by the conversion of cells to spheroplasts, it is not a typical periplasmic protei...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00656a008

    authors： Jacobson GR,Takacs BJ,Rosenbusch JP

    更新日期：1976-06-01 00:00:00

  • Dynamics of ligand binding to alpha-chymotrypsin and to N-methyl-alpha-chymotrypsin.

    abstract：:Ks values for binding of selected substrates, competitive inhibitors, and a noncompetitive inhibitor were found to be similar for alpha-chymotrypsin and N-methyl-alpha-chymotrypsin. The rates and steps of binding of a competitive inhibitor and a noncompetitive inhibitor were also found to be similar for alpha-chymotry...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00262a017

    authors： Maehler R,Whitaker JR

    更新日期：1982-09-14 00:00:00

  • Phosphorylation of c-Abl by protein kinase Pak2 regulates differential binding of ABI2 and CRK.

    abstract：:The tyrosine kinase c-Abl is implicated in a variety of cellular processes that are tightly regulated by c-Abl kinase activity and/or by interactions between c-Abl and other signaling molecules. The interaction of c-Abl with the Abl interactor protein Abi2 is shown to be negatively regulated by phosphorylation of seri...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi701533j

    authors： Jung JH,Pendergast AM,Zipfel PA,Traugh JA

    更新日期：2008-01-22 00:00:00

  • Structure around the cleavage site in the thrombin receptor determined by NMR spectroscopy.

    abstract：:NMR spectroscopic experiments were performed to study the structure of synthetic peptides identical to two extracellular regions of the human thrombin receptor. The smaller molecule, comprising 14 amino acids, was biologically active and was equivalent to the "tethered ligand" exposed after cleavage of the receptor by...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00186a005

    authors： Smith KJ,Trayer IP,Grand RJ

    更新日期：1994-05-24 00:00:00

  • T4 RNA Ligase joins 2'-deoxyribonucleoside 3',5'-bisphosphates to oligodeoxyribonucleotides.

    abstract：:T4 RNA ligase catalyzes the ATP-dependent addition of a single 2'-deoxyribonucleoside 3',5'-bisphosphate to the 3'-hydroxyl of an oligodeoxyribonucleotide. The bisphosphate is joined to the deoxyoligomer by a 3' leads to 5' phosphodiester bond and the product, which is terminated by a 3'-phosphate, is one nucleotide l...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00617a004

    authors： Hinton DM,Baez JA,Gumport RI

    更新日期：1978-11-28 00:00:00

  • Photoaffinity labeling of oxidosqualene cyclase and squalene cyclase by a benzophenone-containing inhibitor.

    abstract：:A new orally active oxidosqualene:lanosterol cyclase (OSLC) inhibitor (Ro48-8071; Morand, O. H. et al. (1997) J. Lipid Res. 38, 373-390) showed potent noncompetitive inhibition of bacterial squalene:hopene cyclase (SHC) from Alicyclobacillus acidocaldarius (IC50 = 9.0 nM, KI = 6.6 nM) and OSLC (IC50 = 40 nM, KI = 22 n...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi980366c

    authors： Abe I,Zheng YF,Prestwich GD

    更新日期：1998-04-28 00:00:00

  • Solution conformation of asparagine-linked oligosaccharides: alpha(1-2)-, alpha(1-3)-, beta(1-2)-, and beta(1-4)-linked units.

    abstract：:The solution conformation is presented for representatives of each of the major classes of asparaginyl oligosaccharides. In this report the conformation of alpha(1-3)-, alpha(1-2)-, beta(1-2)-, and beta(1-4)-linked units is described. The conformational properties of these glycopeptides were determined by high-resolut...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00284a021

    authors： Brisson JR,Carver JP

    更新日期：1983-07-19 00:00:00

  • Delta T 14/Delta D 15 Azotobacter vinelandii ferredoxin I: creation of a new CysXXCysXXCys motif that ligates a [4Fe-4S] cluster.

    abstract：:In clostridial-type ferredoxins, each of the two [4Fe-4S]2+/+ clusters receives three of its four ligands from a CysXXCysXXCys motif. Azotobacter vinelandii ferredoxin I (AvFdI) is a seven-iron ferredoxin that contains one [4Fe-4S]2+/+ cluster and one [3Fe-4S]+/0 cluster. During the evolution of the 7Fe azotobacter-ty...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9810499

    authors： Kemper MA,Gao-Sheridan HS,Shen B,Duff JL,Tilley GJ,Armstrong FA,Burgess BK

    更新日期：1998-09-15 00:00:00

  • Gonadotropin releasing hormone activates the lipoxygenase pathway in cultured pituitary cells: role in gonadotropin secretion and evidence for a novel autocrine/paracrine loop.

    abstract：:The formation and role of arachidonic acid (AA) and its metabolites during gonadotropin releasing hormone- (GnRH-) induced gonadotropin secretion were investigated in primary cultures of rat pituitary cells. Prelabeled cells ([3H]AA) responded to GnRH challenge with increased formation (about 2-fold) of the leukotrien...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00139a004

    authors： Dan-Cohen H,Sofer Y,Schwartzman ML,Natarajan RD,Nadler JL,Naor Z

    更新日期：1992-06-23 00:00:00

  • Endogenous K+/H+ exchange activity in the Sf9 insect cell line.

    abstract：:The fluorescent pH indicator 2',7'-bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF) was used to investigate changes in the intracellular pH (pHi) of individual Sf9 cells in response to changes in the composition of the external medium. Under standard conditions, the resting pHi was 0.2-0.3 unit lower than the extracel...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00046a023

    authors： Vachon V,Paradis MJ,Marsolais M,Schwartz JL,Laprade R

    更新日期：1995-11-21 00:00:00

  • Phosphorylation and functions of inhibitor-2 family of proteins.

    abstract：:Protein phosphatase-1 (PP1) is an essential protein Ser/Thr phosphatase that is extraordinarily conserved from yeast to human, and Inhibitor-2 (I-2) is the most ancient of the heat-stable proteins specific for PP1. We identified novel I-2 homologues in Caenorhabditis elegans (Ce) and Xenopus laevis (Xe) and compared t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi602369m

    authors： Li M,Satinover DL,Brautigan DL

    更新日期：2007-03-06 00:00:00

  • Effect of phospholipid oxidation products on transbilayer movement of phospholipids in single lamellar vesicles.

    abstract：:Single lamellar phosphatidyl[methyl-2H]choline vesicles were incubated with an excess of unlabeled phosphatidylcholine vesicles or phosphatidylcholine-cholesterol vesicles containing 8 mol % glucuronosyldiglyceride. Incubation of the two vesicle populations was performed in the presence or absence of a purified phosph...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00534a017

    authors： Shaw JM,Thompson TE

    更新日期：1982-03-02 00:00:00

  • 1H NMR dipolar echo decay spectroscopy: a sensitive probe of membrane structure.

    abstract：:The structural and motional properties of membrane lipids in various physical states and macroscopic organizations are elucidated by 1H NMR dipolar echo decay spectroscopy (DECODE). Multilamellar lipid dispersions in the gel (L beta') and liquid-crystalline (L alpha) states and a nonbilayer, hexagonal (HII) phase are ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00488a027

    authors： Janes N,Rubin E,Taraschi TF

    更新日期：1990-09-11 00:00:00

  • Role of protein-water interactions and electrostatics in alpha-synuclein fibril formation.

    abstract：:Deposition of misfolded alpha-synuclein is a critical factor in several neurodegenerative disorders. Filamentous alpha-synuclein is the major component of Lewy bodies and Lewy neurites, the intracellular inclusions in the dopaminergic neurons of the substantia nigra, which are considered the pathological hallmark of P...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi034938r

    authors： Munishkina LA,Henriques J,Uversky VN,Fink AL

    更新日期：2004-03-23 00:00:00

  • In vitro inhibition of MAP kinase (ERK1/ERK2) activity by phosphorylated glia maturation factor (GMF).

    abstract：:We report that recombinant glia maturation factor (GMF), a 17-kDa brain protein, inhibits the activity of mitogen-activated protein (MAP) kinase in the test tube assay, in particular the ERK1/ERK2 isoforms. A preliminary phosphorylation of GMF by protein kinase A (PKA) dramatically increases its inhibitory effect by o...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi960034c

    authors： Zaheer A,Lim R

    更新日期：1996-05-21 00:00:00

  • Involvement of tryptophan residues at the coenzyme A binding site of carbon monoxide dehydrogenase from Clostridium thermoaceticum.

    abstract：:Carbon monoxide dehydrogenase (CODH) from Clostridium thermoaceticum plays a central role in the newly discovered acetyl-CoA pathway [Wood, H.G., Ragsdale, S.W., & Pezacka, E. (1986) FEMS Microbiol. Rev. 39, 345-362]. The enzyme catalyzes the formation of acetyl-CoA from methyl, carbonyl, and CoA groups, and it has sp...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00417a045

    authors： Shanmugasundaram T,Kumar GK,Wood HG

    更新日期：1988-08-23 00:00:00

  • Emerging Functional Differences between the Synaptotagmin and Ferlin Calcium Sensor Families.

    abstract：:The ferlin family proteins have emerged as multi-C2 domain regulators of calcium-triggered membrane fusion and fission events. While initially determined to share many of the features of members of the synaptotagmin family of calcium sensors, ferlins in more recent studies have been found to interact directly with non...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.7b00928

    authors： Johnson CP

    更新日期：2017-12-12 00:00:00

  • Structural and biochemical studies of a fluoroacetyl-CoA-specific thioesterase reveal a molecular basis for fluorine selectivity.

    abstract：:We have initiated a broad-based program aimed at understanding the molecular basis of fluorine specificity in enzymatic systems, and in this context, we report crystallographic and biochemical studies on a fluoroacetyl-coenzyme A (CoA) specific thioesterase (FlK) from Streptomyces cattleya. Our data establish that FlK...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi101102u

    authors： Weeks AM,Coyle SM,Jinek M,Doudna JA,Chang MC

    更新日期：2010-11-02 00:00:00

  • Direct visualization of the binding of c-Myc/Max heterodimeric b-HLH-LZ to E-box sequences on the hTERT promoter.

    abstract：:Myc and Max belong to the b-HLH-LZ family of transcription factors. Heterodimerization between Myc and Max or homodimerization of Max allows these proteins to bind their cognate DNA sequence known as the E-box (CACGTG). Recent evidence has suggested that the c-Myc/Max heterodimeric b-HLH-LZ could interact to form a he...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi700076m

    authors： Lebel R,McDuff FO,Lavigne P,Grandbois M

    更新日期：2007-09-11 00:00:00

  • Membrane Docking of the Synaptotagmin 7 C2A Domain: Electron Paramagnetic Resonance Measurements Show Contributions from Two Membrane Binding Loops.

    abstract：:The synaptotagmin (Syt) family of proteins plays an important role in vesicle docking and fusion during Ca(2+)-induced exocytosis in a wide variety of cell types. Its role as a Ca(2+) sensor derives primarily from its two C2 domains, C2A and C2B, which insert into anionic lipid membranes upon binding Ca(2+). Syt isofo...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b00421

    authors： Osterberg JR,Chon NL,Boo A,Maynard FA,Lin H,Knight JD

    更新日期：2015-09-22 00:00:00

  • Biochemical basis for the functional switch that regulates hepatocyte growth factor receptor tyrosine kinase activation.

    abstract：:Ligand-induced dimerization of receptor tyrosine kinases (RTKs) modulates a system of linked biochemical reactions, sharply switching the RTK from a quiescent state to an active state that becomes phosphorylated and triggers intracellular signaling pathways. To improve our understanding of this molecular switch, we de...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi701892f

    authors： Sheth PR,Hays JL,Elferink LA,Watowich SJ

    更新日期：2008-04-01 00:00:00

  • Studies of synthetic peptides of human apolipoprotein A-I containing tandem amphipathic alpha-helixes.

    abstract：:In mature human apolipoprotein A-I (apo A-I), the amino acid residues 1-43 are encoded by exon 3, whereas residues 44-243 are encoded by exon 4 of the apo A-I gene. The region encoded by exon 4 of the apo A-I gene contains 10 tandem amphipathic alpha-helixes; their location and the class to which they belong are as fo...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi980042o

    authors： Mishra VK,Palgunachari MN,Datta G,Phillips MC,Lund-Katz S,Adeyeye SO,Segrest JP,Anantharamaiah GM

    更新日期：1998-07-14 00:00:00

  • Conformation of a rigid tetrasaccharide epitope in the capsular polysaccharide of Vibrio cholerae O139.

    abstract：:A newly reported strain of Vibrio cholerae, known as strain O139 Bengal, is the first instance of an encapsulated strain that has caused epidemic cholera. The O-antigenic capsule is the critical antigen for protective immunity. Since mapping of the antigenic epitopes will assist in the development of a protein conjuga...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9910272

    authors： Gunawardena S,Fiore CR,Johnson JA,Bush CA

    更新日期：1999-09-14 00:00:00

  • Estimating the degree of expansion in the transition state for protein unfolding: analysis of the pH dependence of the rate constant for caricain denaturation.

    abstract：:The thermal denaturation of caricain (the most alkaline of papain-related proteinases) was studied in acid media. Under all conditions tested, caricain denatured irreversibly following a single first-order reaction that involves simultaneous loss of secondary and tertiary structures. Besides, variation of the rate con...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi991658w

    authors： López-Arenas L,Solís-Mendiola S,Hernández-Arana A

    更新日期：1999-11-30 00:00:00

  • The crystal structure of the Escherichia coli maltodextrin phosphorylase-acarbose complex.

    abstract：:Acarbose is a naturally occurring pseudo-tetrasaccharide. It has been used in conjunction with other drugs in the treatment of diabetes where it acts as an inhibitor of intestinal glucosidases. To probe the interactions of acarbose with other carbohydrate recognition enzymes, the crystal structure of E. coli maltodext...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9828573

    authors： O'Reilly M,Watson KA,Johnson LN

    更新日期：1999-04-27 00:00:00

  • Escherichia coli cAMP receptor protein-DNA complexes. 1. Energetic contributions of half-sites and flanking sequences in DNA recognition.

    abstract：:In Escherichia coli, the cyclic AMP receptor protein (CRP) serves as a sensor of the intracellular level of cyclic AMP. At increasing concentrations of cyclic AMP, CRP becomes activated upon binding a cyclic AMP molecule. The activated CRP is capable of regulating the expression of more than 20 genes by binding to spe...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi972450i

    authors： Pyles EA,Chin AJ,Lee JC

    更新日期：1998-04-14 00:00:00

  • Investigation of the origin of the "S3" EPR signal from the oxygen-evolving complex of photosystem 2: the role of tyrosine Z.

    abstract：:The origin of the "S3" EPR signal from calcium-depleted photosystem 2 samples has been investigated. This signal is observed after freezing samples under illumination and has been assigned to an interaction between the manganese cluster and an oxidized histidine radical [Boussac et al. (1990) Nature 347; 303-306]. In ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00134a005

    authors： Hallahan BJ,Nugent JH,Warden JT,Evans MC

    更新日期：1992-05-19 00:00:00

  • Cold denaturation of the molten globule states of apomyoglobin and a profile for protein folding.

    abstract：:Protein folding is a process in which an extended polypeptide chain acquires compact packing through the formation of specific secondary and tertiary structures and hydrophobic interactions. Although much attention has been paid to secondary and tertiary structures, there is no definitive view about the relationship b...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00182a019

    authors： Nishii I,Kataoka M,Tokunaga F,Goto Y

    更新日期：1994-04-26 00:00:00