Abstract：
:Single lamellar phosphatidyl[methyl-2H]choline vesicles were incubated with an excess of unlabeled phosphatidylcholine vesicles or phosphatidylcholine-cholesterol vesicles containing 8 mol % glucuronosyldiglyceride. Incubation of the two vesicle populations was performed in the presence or absence of a purified phosphatidylcholine exchange protein. The negatively charged glycolipid donor vesicles could be completely removed by column chromatography on DEAE-Sephacel. Following incubation with exchange protein and subsequent fractionation, the -N(CD3)3 phosphatidylcholine acceptor vesicles exhibited a 61-73% enrichment of the unlabeled phosphatidylcholine in the outer monolayer. Upon incubation in an air atmosphere, no appreciable transbilayer movement of the outer monolayer -N(CH3)3 phosphatidylcholine was observed for at least 5 days. Between days 5 and 7, however, extensive transbilayer movement occurred, leading to an outer monolayer/inner monolayer phosphatidylcholine ratio of 2.1 on day 7. In phosphatidylcholine-6 mol % cholesterol vesicles treated similarly, the outside/inside ratio of the unlabeled phospholipid was 6.7, suggesting a much smaller percentage of transbilayer movement. The loss of transbilayer asymmetry which occurred during a 36-h period after day 5 could be estimated at the upper limit, t 1/2 approximately 7.3 h for phosphatidylcholine vesicles and t 1/2 approximately 53 h for phosphatidylcholine-cholesterol vesicles. The actual rates for transbilayer movement, however, were likely more rapid. Transbilayer movement occurred at a time period when oxidized phospholipid breakdown products had reached critical levels.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Shaw JM,Thompson TEdoi
10.1021/bi00534a017subject
Has Abstractpub_date
1982-03-02 00:00:00pages
920-7issue
5eissn
0006-2960issn
1520-4995journal_volume
21pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Clostridial neurotoxins are zinc endopeptidases, and each contains one Zn(2+)/molecule. To investigate the structural/functional role of Zn(2+) in botulinum neurotoxin light chain (the enzymatic subunit of the neurotoxin), the effect of the removal of zinc on protein folding and enzyme kinetics was investigated. The a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0007472
更新日期：2000-08-29 00:00:00
abstract：:Human alkyladenine DNA glycosylase (AAG) locates and excises a wide variety of structurally diverse alkylated and oxidized purine lesions from DNA to initiate the base excision repair pathway. Recognition of a base lesion requires flipping of the damaged nucleotide into a relatively open active site pocket between two...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi101856a
更新日期：2011-03-22 00:00:00
abstract：:SHAPE-MaP is unique among RNA structure probing strategies in that it both measures flexibility at single-nucleotide resolution and quantifies the uncertainties in these measurements. We report a straightforward analytical framework that incorporates these uncertainties to allow detection of RNA structural differences...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00977
更新日期：2015-11-24 00:00:00
abstract：:Aquifex aeolicus 3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS) is active with a variety of different divalent metal ions bound in the active site. The Cd(2+), Zn(2+), and Cu(2+) substituted enzymes display similar values of k(cat) and similar dependence of K(m)(PEP) and K(m)(A5P) on both substrate and pro...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi801955h
更新日期：2009-04-28 00:00:00
abstract：:The electron spin resonance probes 5-doxylstearate and 4-(dodecyldimethylammonio)-1-oxy-2,2,6,6-tetramethylpiperidine bromide were used to characterize the fluidity of the acyl chain and head-group regions, respectively, of defined salts of lipopolysaccharide (LPS) from Escherichia coli K12. The removal of the weakly ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00277a042
更新日期：1983-04-12 00:00:00
abstract：:Recent studies have indicated that CYP3A4 exhibits non-Michaelis-Menten kinetics for numerous substrates. Both homo- and heterotropic activation have been reported, and kinetic models have suggested multiple substrates within the active site. We provide some of the first physicochemical data supporting the hypothesis ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi051689t
更新日期：2005-11-01 00:00:00
abstract：:A wide variety of biological processes including differentiation, regeneration, and cancer progression are regulated by shedding of membrane-anchored proteins. One of the major sheddases is A Disintegrin And Metalloprotease-17 (ADAM17) whose extracellular region consists of a pro-, a catalytic, a disintegrin-, and a m...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00497
更新日期：2015-09-29 00:00:00
abstract：:Misfolded proteins in the endoplasmic reticulum (ER) are degraded by N-terminal threonine proteases within the 26S proteasome. Each protease is formed by an activated beta subunit, beta5/X, beta1/Y, or beta2/Z, that exhibits chymotrypsin-like, peptidylglutamyl-peptide hydrolyzing, or trypsin-like activity, respectivel...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi011322y
更新日期：2001-11-06 00:00:00
abstract：:Chemical biosensors, for which chemical detection triggers a fluorescent signal, have the potential to accelerate the screening of noncolorimetric chemicals produced by microbes, enabling the high-throughput engineering of enzymes and metabolic pathways. Here, we engineer a G-protein-coupled receptor (GPCR)-based sens...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b00605
更新日期：2017-10-17 00:00:00
abstract：:Protein tyrosine kinases (PTKs) are important regulators of mammalian cell function and their own activities are tightly regulated. Underlying their tight regulation, all PTKs contain multiple regulatory domains in addition to a catalytic domain. C-terminal Src kinase (Csk) contains a catalytic domain and a regulatory...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi048142j
更新日期：2005-02-08 00:00:00
abstract：:Rat mucosal-type mast cells of the RBL-2H3 line express a glycoprotein termed the MAst cell Function-associated Antigen (MAFA). When MAFA is clustered by its specific monoclonal antibody G63, secretion normally triggered by aggregating these cells' type I Fcepsilon receptor (FcepsilonRI) is substantially inhibited. Th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi011566i
更新日期：2002-01-22 00:00:00
abstract：:Arginase is a binuclear Mn(2+) metalloenzyme that catalyzes the hydrolysis of L-arginine to L-ornithine and urea. X-ray crystal structures of arginase complexed to substrate analogues N(omega)-hydroxy-L-arginine and N(omega)-hydroxy-nor-L-arginine, as well as the products L-ornithine and urea, complete a set of struct...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi002318+
更新日期：2001-03-06 00:00:00
abstract：:The early redox events involved in iron reduction and mobilization in mammalian ferritin have been investigated by several techniques. Sedimentation velocity measurements of ferritin samples with altered core sizes, prepared by partial reduction and Fe2+ chelation, suggest two different events occur during iron loss f...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00430a033
更新日期：1989-02-21 00:00:00
abstract：:PDZ (acronym of the synapse-associated protein PSD-95/SAP90, the septate junction protein Discs-large, and the tight junction protein ZO-1) domains are abundant small globular protein interaction domains that mainly recognize the carboxyl termini of their target proteins. Detailed knowledge on PDZ domain binding speci...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi700954e
更新日期：2007-11-27 00:00:00
abstract：:SopE and SopE2 are delivered by the Salmonella type III secretion system into eukaryotic cells to promote cell invasion. SopE and SopE2 are potent guanine nucleotide exchange factors (GEFs) for Rho GTPases Cdc42 and Rac1 and constitute a novel class of Rho GEFs. Although the sequence of SopE-like GEFs is not at all ho...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0490744
更新日期：2004-09-28 00:00:00
abstract：:Furamidine and related diamidines represent a promising series of drugs active against widespread parasites, in particular the Pneumocystic carinii pathogen. In this series, the phenylfuranbenzimidazole diamidine derivative DB293 was recently identified as the first unfused aromatic dication capable of forming stacked...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0108453
更新日期：2001-08-21 00:00:00
abstract：:We investigated how the intrinsic sequence-dependent properties probed via the phosphate linkages (BI ↔ BII equilibrium) influence the preferred shape of free DNA, and how this affects the nucleosome formation. First, this exploits NMR solution studies of four B-DNA dodecamers that together cover 39 base pairs of the ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi500504y
更新日期：2014-09-09 00:00:00
abstract：:Terreic acid is a metabolite with antibiotic properties produced by the fungus Aspergillus terreus. We found that terreic acid is a covalent inhibitor of the bacterial cell wall biosynthetic enzyme MurA from Enterobacter cloacae and Escherichia coli in vitro. The crystal structure of the MurA dead-end complex with ter...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi100365b
更新日期：2010-05-18 00:00:00
abstract：:The L-type Ca(2+) channel Ca(v)1.2 forms macromolecular signaling complexes that comprise the β(2) adrenergic receptor, trimeric G(s) protein, adenylyl cyclase, and cAMP-dependent protein kinase (PKA) for efficient signaling in heart and brain. The protein phosphatases PP2A and PP2B are part of this complex. PP2A coun...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi101018c
更新日期：2010-12-07 00:00:00
abstract：:GTPases are key players during cellular signaling. Phosphorylation of Rab proteins, which belong to the Ras superfamily of small GTPases regulating intracellular transport, has been implicated in the pathogenesis of Parkinson's disease. For Rab8a, it was shown that serine 111 phosphorylation (pS111) is dependent on th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.9b00516
更新日期：2019-08-20 00:00:00
abstract：:Cyanobacteriochromes (CBCRs) are photosensory proteins related to the red/far-red phytochromes. Like phytochromes, CBCRs use linear tetrapyrrole (bilin) chromophores covalently attached via a thioether linkage to a conserved Cys residue also found in plant and cyanobacterial phytochromes. Unlike almost all phytochrome...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300171s
更新日期：2012-05-01 00:00:00
abstract：:Three spin-labeled derivatives of the voltage-gated peptide alamethicin were prepared with nitroxides at the C-terminal phenyalaninol, and at positions 9 and 15 in the amino acid sequence. In addition, three spin-labeled derivatives of an analog of alamethicin where alpha-methylalanine residues are replaced by leucine...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi951985d
更新日期：1996-01-16 00:00:00
abstract：:The membrane interaction properties of two single-residue variants, R6W and L5S, of the 17-amino acid neuropeptide dynorphin A (DynA) were studied by circular dichroism (CD) and nuclear magnetic resonance (NMR) spectroscopy. Corresponding gene mutations have recently been discovered in humans and causatively linked to...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi4004205
更新日期：2013-06-18 00:00:00
abstract：:The NikR transcription factor from Escherichia coli is a Ni(II)-dependent repressor that regulates production of the nickel ion transporter encoded by the nik operon. In the previous paper in this issue (Wang, S. C., Dias, A., Bloom, S. L., and Zamble, D. B. (2004) Selectivity of Metal Binding and Metal-Induced Stabil...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049404k
更新日期：2004-08-10 00:00:00
abstract：:The Pseudomonas aeruginosa FpvA receptor is a TonB-dependent outer membrane transport protein that catalyzes uptake of ferric pyoverdin across the outer membrane. Surprisingly, FpvA expressed in P. aeruginosa grown in an iron-deficient medium copurifies with a ligand X that we have characterized by UV, fluorescence, a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi990421x
更新日期：1999-07-20 00:00:00
abstract：:It has been suggested that the down-regulation of hepatic HMG-CoA reductase by dietary cholesterol requires modification of the cholesterol molecule before it can exert its suppressive action. In a recent study [Lund, E., Breuer, O., & Björkhem, I. (1992) J. Biol. Chem. 267, 25092-25097], we showed that side-chain hyd...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00167a038
更新日期：1994-01-11 00:00:00
abstract：:Site-directed mutants of human carbonic anhydrase III were used to examine the role of Thr-199 and its interaction with Phe-198 in the catalyzed hydration of CO2. Threonine-199 is a hydrogen bond acceptor for the zinc-bound water, and Phe-198 forms part of the hydrophobic side of the active-site cavity of carbonic anh...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00082a004
更新日期：1993-08-10 00:00:00
abstract：:Molecular chaperones facilitate the folding and assembly of proteins and inhibit their aberrant aggregation. They thus offer several opportunities for biomedical and biotechnological applications, as for example they can often prevent protein aggregation more effectively than other therapeutic molecules, including sma...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00459
更新日期：2015-08-18 00:00:00
abstract：:Receptor tyrosine kinase (RTK) activation is associated with modulation of heptahelical receptor-stimulated adenylyl cyclase responses. The mechanisms underlying the RTK-mediated enhancement of adenylyl cyclase function remain unclear. In the present studies, we show that the tyrosine kinase-dependent enhancement of a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0015818
更新日期：2001-02-13 00:00:00
abstract：:Steroid-receptor complexes formed at low temperature and ionic strength are unable to bind to target cell nuclei. After a temporary exposure to high ionic strength and/or temperature they become activated (i.e., able to bind to nuclei). However, there exists an equilibrium between activated and nonactivated complexes;...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00664a025
更新日期：1976-09-21 00:00:00
