An allosteric intramolecular PDZ-PDZ interaction modulates PTP-BL PDZ2 binding specificity.

abstract：:The method of transient electric birefringence has been applied to study the conformation of protein-sodium dodecyl sulfate complexes. A model of a deformable prolate ellipsoid has been proposed for the protein-dodecyl sulfate complex. This model is compared to the models proposed by J. A. Reynolds and C. Tanford (197...

journal_title：Biochemistry

authors： Wright AK,Thompson MR,Miller RL

更新日期：1975-07-15 00:00:00

abstract：:Low-temperature electronic absorption and room temperature circular dichroism (CD) and magnetic circular dichroism (MCD) spectra are reported for ascorbate oxidase. Bands attributable to d-d electronic transitions in the type 1 (blue) coppers (2B2 ground state) have been observed at 5800, 10000, and 12000 cm-1 (1725, ...

journal_title：Biochemistry

authors： Dooley DM,Dawson JH,Stephens PJ,Gray HB

更新日期：1981-03-31 00:00:00

abstract：:The unique characteristics of DNA charge transport (CT) have prompted an examination of roles for this chemistry within a biological context. Not only can DNA CT facilitate long-range oxidative damage of DNA, but redox-active proteins can couple to the DNA base stack and participate in long-range redox reactions using...

journal_title：Biochemistry

authors： Grodick MA,Muren NB,Barton JK

更新日期：2015-02-03 00:00:00

abstract：:The three-dimensional high-resolution structure of rhodopsin is unknown, as is the case for almost all integral membrane patients. As part of an alternative approach to determine of membrane protein structure, we are pursuing the structure of cytoplasmic domains of this G-protein receptor. A peptide, rhoIII, with the ...

journal_title：Biochemistry

authors： Yeagle PL,Alderfer JL,Albert AD

更新日期：1995-11-14 00:00:00

abstract：:The ErbB family of tyrosine kinase receptors is a key element in preserving cell growth homeostasis. This family is comprised of four single-transmembrane domain proteins designated ErbB-1-4. Ligand binding initiates dimerization followed by tyrosine phosphorylation and signaling, which when uncontrolled lead to cance...

journal_title：Biochemistry

authors： Bublil EM,Cohen T,Arnusch CJ,Peleg A,Pines G,Lavi S,Yarden Y,Shai Y

更新日期：2016-10-04 00:00:00

abstract：:The ability to undergo large-scale domain rearrangements is essential for the substrate-binding function of periplasmic binding proteins (PBPs), which are indispensable for nutrient uptake in Gram-negative bacteria. Crystal structures indicate that PBPs typically adopt either an "open" unliganded configuration or a "c...

journal_title：Biochemistry

authors： Bermejo GA,Strub MP,Ho C,Tjandra N

更新日期：2010-03-09 00:00:00

abstract：:Serotonergic signaling appears to play a key role in the generation and modulation of various cognitive, behavioral, and developmental processes. The serotonin(1A) receptor is an important member of the superfamily of seven transmembrane domain G-protein-coupled receptors and is the most extensively studied among the ...

journal_title：Biochemistry

authors： Pucadyil TJ,Kalipatnapu S,Harikumar KG,Rangaraj N,Karnik SS,Chattopadhyay A

更新日期：2004-12-21 00:00:00

abstract：:Despite a number of studies, the formation of the Michaelis complexes between ferredoxin-NADP (+) reductases and NADP(H) eluded detailed investigations by rapid kinetic techniques because of their high formation rates. Moreover, the reversible nature of the reaction of hydride ion transfer between these enzymes and NA...

journal_title：Biochemistry

authors： Pennati A,Zanetti G,Aliverti A,Gadda G

更新日期：2008-03-18 00:00:00

abstract：:We extended the technique of frequency-domain fluorometry to an upper frequency limit of 2000 MHz. This was accomplished by using the harmonic content of a laser pulse train (3.76 MHz, 5 ps) from a synchronously pumped and cavity-dumped dye laser. We used a microchannel plate photomultiplier as the detector to obtain ...

journal_title：Biochemistry

authors： Lakowicz JR,Laczko G,Gryczynski I

更新日期：1987-01-13 00:00:00

abstract：:Crystallographic analysis revealed that the nicotinamide ring of NAD can bind with multiconformations to aldehyde dehydrogenase (ALDH) (Ni, L., Zhou, J., Hurley, T. D., and Weiner, H. (1999) Protein Sci. 8, 2784-2790). Electron densities can be defined for two conformations, neither of which appears to be compatible w...

journal_title：Biochemistry

authors： Hammen PK,Allali-Hassani A,Hallenga K,Hurley TD,Weiner H

更新日期：2002-06-04 00:00:00

abstract：:Although host protein synthesis is preferentially inhibited, there is a steady decline in the ability of Chinese hamster ovary (CHO) cells infected with vesicular stomatitis virus (VSV) to synthesize both host and viral proteins. We previously reported finding an mRNA-ribonucleoprotein particle (mRNP) that contained a...

journal_title：Biochemistry

authors： Rosen CA,Siekierka J,Ennis HL,Cohen PS

更新日期：1984-05-22 00:00:00

abstract：:The virally encoded integrase protein carries out retroviral integration, which requires specific interactions with the two ends of the viral DNA, and also with host DNA that is the target of integration. We attached a photo-cross-linking agent to specific viral and target DNA sites to identify regions of the integras...

journal_title：Biochemistry

authors： Heuer TS,Brown PO

更新日期：1998-05-12 00:00:00

abstract：:Proteins from the enhanced intracellular survival (Eis) family are versatile acetyltransferases that acetylate amines at multiple positions of several aminoglycosides (AGs). Their upregulation confers drug resistance. Homologues of Eis are present in diverse bacteria, including many pathogens. Eis from Mycobacterium t...

journal_title：Biochemistry

authors： Green KD,Biswas T,Chang C,Wu R,Chen W,Janes BK,Chalupska D,Gornicki P,Hanna PC,Tsodikov OV,Joachimiak A,Garneau-Tsodikova S

更新日期：2015-05-26 00:00:00

abstract：:A faithful transcription system for ribosomal RNA genes has been developed by using components from the small free-living amoeba Acanthamoeba castellanii. The system utilizes protein-free recombinant DNA as a template and in addition requires a crude cell-free extract containing RNA polymerase I and a transcription in...

journal_title：Biochemistry

authors： Paule MR,Iida CT,Perna PJ,Harris GH,Brown Shimer SL,Kownin P

更新日期：1984-08-28 00:00:00

abstract：:A conserved, crystallographically defined bile acid binding site was originally identified in the membrane domain of mammalian and bacterial cytochrome c oxidase (CcO). Current studies show other amphipathic molecules including detergents, fatty acids, steroids, and porphyrins bind to this site and affect the already ...

journal_title：Biochemistry

authors： Hiser C,Buhrow L,Liu J,Kuhn L,Ferguson-Miller S

更新日期：2013-02-26 00:00:00

abstract：:Human protein Z is a vitamin K-dependent plasma glycoprotein, deficiency of which leads to a mild bleeding tendency. Protein Z appears to assist hemostasis by binding thrombin and promoting its association with phospholipid vesicles. In this study, to characterize the gene for protein Z, its organization and structure...

journal_title：Biochemistry

authors： Fujimaki K,Yamazaki T,Taniwaki M,Ichinose A

更新日期：1998-05-12 00:00:00

abstract：:As a prerequisite to mammalian fertilization, the sperm acrosomal vesicle fuses with the plasma membrane and the acrosome contents are exocytosed. Induction occurs through engagement of the sperm receptors by multiple sugar residues. Multivalent polymers displaying mannose, fucose, or GlcNAc are effective synthetic in...

journal_title：Biochemistry

authors： Huang H,Rodolis MT,Bhatia SR,Sampson NS

更新日期：2017-06-06 00:00:00

abstract：:Neutral glycosphingolipids and gangliosides in rabbit thymus, spleen, bone marrow, and erythrocyte ghosts were analyzed by conventional chemical and enzymatic procedures and negative ion fast atom bombardment mass spectrometry (FABMS). Thymus gangliosides showed a characteristic composition. Major gangliosides compris...

journal_title：Biochemistry

authors： Iwamori M,Kiguchi K,Kanno J,Kitagawa M,Nagai Y

更新日期：1986-02-25 00:00:00

abstract：:West Nile virus (WNV) is a mosquito-borne flavivirus with a rapidly expanding global distribution. Infection can cause severe neurological disease and fatality in humans. Efforts are ongoing to develop antiviral drugs that inhibit the WNV protease, a viral enzyme required for polyprotein processing. Unfortunately, lit...

journal_title：Biochemistry

authors： Garces AP,Watowich SJ

更新日期：2013-10-01 00:00:00

abstract：:A single histidine residue has been placed at either the N-terminus or the C-terminus of each of the two alpha-helices of barnase. The pKa of that histidine residue in each of the four mutants has been determined by 1H NMR. The pKas of the two residues at the C-terminus are, on average, 0.5 unit higher, and those of t...

journal_title：Biochemistry

authors： Sancho J,Serrano L,Fersht AR

更新日期：1992-03-03 00:00:00

abstract：:Recent experiments [Wells, J., & Yount, R. (1979) Proc. Natl. Acad. Sci. U.S.A. 76, 4966] have shown it is possible to trap MgADP and other nucleotides stably at the active site of myosin by cross-linking two thiol groups. A variety of cross-linking reagents including chelation of the two thiols by cobalt (III) phenan...

journal_title：Biochemistry

authors： Dalbey RE,Wells JA,Yount RG

更新日期：1983-01-18 00:00:00

abstract：:The partition of cis-parinaric acid (9,11,13,15-cis, trans, trans,cis-octadecatetraenoic acid, cis-PnA) and trans-parinaric acid (9,11,13,15-all-trans-octadecatetraenoic acid, trans-PnA) among aqueous, solid lipid, and fluid lipid phases has been measured by three spectroscopic parameters: absorption spectral shifts, ...

journal_title：Biochemistry

authors： Sklar LA,Miljanich GP,Dratz EA

更新日期：1979-05-01 00:00:00

abstract：:Avian magnetoreception is assumed to occur in the retina. Although its molecular mechanism is unclear, magnetic field-dependent formation and the stability of radical-containing photointermediate(s) are suggested to play key roles in a hypothesis called the radical pair mechanism. Chicken cryptochrome4 (cCRY4) has bee...

journal_title：Biochemistry

authors： Otsuka H,Mitsui H,Miura K,Okano K,Imamoto Y,Okano T

更新日期：2020-09-29 00:00:00

abstract：:Apurinic/apyrimidinic endonuclease 1 (APE1) is a DNA repair protein and plays other important roles. Increased levels of APE1 in cancer have been reported. However, available methods for measuring APE1 levels are indirect and not quantitative. We previously developed an approach using liquid chromatography and tandem ...

journal_title：Biochemistry

authors： Coskun E,Jaruga P,Reddy PT,Dizdaroglu M

更新日期：2015-09-29 00:00:00

abstract：:The reactions of fully reduced and mixed-valence bovine heart cytochrome c oxidase with dioxygen have been reinvestigated in the absence and presence of metal ions (Zn(2+), Ni(2+), and Cd(2+)) by time-resolved optical absorption spectroscopy using the CO flow-flash technique. The time-resolved data were recorded on a ...

journal_title：Biochemistry

authors： Szundi I,Cappuccio J,Einarsdóttir O

更新日期：2004-12-21 00:00:00

abstract：:5-Hydroxy-1,2-naphthalenedicarboxylic anhydride is closely related to its precursor dibasic acid which is a metabolite of the carcinogenic polynuclear hydrocarbon dibenz[a,h]anthracene. The anhydride inhibited respiration of coupled mitochondria. This inhibition was relieved by 2,4-dinitrophenol. Several mitochondrial...

journal_title：Biochemistry

authors： Hadler HI,Demetriou JM

更新日期：1975-12-02 00:00:00

abstract：:Rat brain and kidney cDNA libraries were constructed and screened with a cDNA insert corresponding to the mRNA for the sheep kidney Na+,K+-ATPase catalytic subunit. The alpha-subunit cDNAs isolated from the kidney library were derived from a single class of messenger RNA, and the brain cDNAs were derived from three cl...

journal_title：Biochemistry

authors： Shull GE,Greeb J,Lingrel JB

更新日期：1986-12-16 00:00:00

abstract：:Aminoacyl-tRNA synthetases preserve the fidelity of decoding genetic information by accurately joining amino acids to their cognate transfer RNAs. Here, tRNA discrimination at the level of binding by Escherichia coli histidyl-tRNA synthetase is addressed by filter binding, analytical ultracentrifugation, and iodine fo...

journal_title：Biochemistry

authors： Bovee ML,Yan W,Sproat BS,Francklyn CS

更新日期：1999-10-12 00:00:00

abstract：:The association state of the mannitol-specific enzyme II (EIIMtl) has been studied both in the purified form and embedded in the cytoplasmic membrane. Membrane fragments obtained from mannitol-grown Escherichia coli catalyze the phosphoenolpyruvate- (PEP) dependent phosphorylation of both glucose and mannitol; thus th...

journal_title：Biochemistry

authors： Roossien FF,Robillard GT

更新日期：1984-11-20 00:00:00

abstract：:The N-terminal extracellular parts of human G-protein coupled receptor class B, for example, receptors for secretin, glucagon, or parathyroid hormone, are involved in ligand binding. To obtain structural and functional information on the N-terminal receptor fragment of human parathyroid hormone receptor 1 (PTHR1), the...

journal_title：Biochemistry

authors： Grauschopf U,Lilie H,Honold K,Wozny M,Reusch D,Esswein A,Schäfer W,Rücknagel KP,Rudolph R

更新日期：2000-08-01 00:00:00