Abstract：
:Low-temperature electronic absorption and room temperature circular dichroism (CD) and magnetic circular dichroism (MCD) spectra are reported for ascorbate oxidase. Bands attributable to d-d electronic transitions in the type 1 (blue) coppers (2B2 ground state) have been observed at 5800, 10000, and 12000 cm-1 (1725, 1000, and 835 nm). The three bands are assigned to the transitions 2B2 leads to 2E, 2B2 leads to 2B1, and 2B2 leads to 2A1, respectively, in the slightly flattened tetrahedral blue copper centers. The ligand field theoretical treatment predicts a type 1 copper reduction potential of 417 mV. The MCD spectrum of ascorbate oxidase contains one major band at 14000 cm-1 (710 nm) and a very weak feature at 13 200 cm-1 (550 nm). The intensities of the CD and MCD bands are consistent with two or three type 1 coppers in each enzyme molecule, and the near-infrared spectroscopic results suggest that the electronic structures of the blue coppers are closely similar.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Dooley DM,Dawson JH,Stephens PJ,Gray HBdoi
10.1021/bi00510a044subject
Has Abstractpub_date
1981-03-31 00:00:00pages
2024-8issue
7eissn
0006-2960issn
1520-4995journal_volume
20pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Smooth muscle cells maintain filaments of actin and myosin in the presence of ATP, although dephosphorylated myosin filaments and actin-myosin interactions are unstable under those conditions in vitro. Several proteins that stabilize myosin filaments and that stabilize actin-myosin interactions have been identified. F...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi401013g
更新日期：2013-10-29 00:00:00
abstract：:Endothelium-derived epoxyeicosatrienoic acids (EETs) relax vascular smooth muscle by activating potassium channels and causing membrane hyperpolarization. Recent evidence suggests that EETs act via a membrane binding site or receptor. To further characterize this binding site or receptor, we synthesized 20-iodo-14,15-...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi102070w
更新日期：2011-05-10 00:00:00
abstract：:A newly recognized type of protein-ligand interaction phenomenon has resulted in the preparation of simple, nonionic, and highly specific gel derivatives for selective adsorption chromatography. The essential structure of the immobilized ligand can be represented as agarose-CH2CH2SO2CH2CH2SCH2CH2OH, which was prepared...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00396a049
更新日期：1987-11-03 00:00:00
abstract：:Substitution of cysteine for proline-39 in Escherichia coli dihydrofolate reductase by oligonucleotide-directed mutagenesis positions the new cysteine adjacent to already existing cysteine-85. When the mutant protein is expressed in the E. coli cytosol, the cysteine sulfur atoms are found, by X-ray crystallographic an...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00382a017
更新日期：1987-04-21 00:00:00
abstract：:In our previous papers, seven structural genes (NQO1-7) of the energy-transducing NADH-quinone (Q) oxidoreductase of Paracoccus denitrificans were characterized [Xu, X., Matsuno-Yagi, A., & Yagi, T. (1991a) Biochemistry 30, 8678-8684; (1991b) Biochemistry 30, 6422-6428; (1992a) Biochemistry 31, 6925-6932; (1992b) Arch...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00054a030
更新日期：1993-01-26 00:00:00
abstract：:A cDNA designated hIIB1, representing the entire coding sequence of a P450 in the IIB gene family, was isolated from a human liver lambda gt11 library by using the rat IIB1 cDNA as a probe. The hIIB1 protein, deduced from the cDNA sequence, contained 491 amino acids, had a calculated molecular weight of 56,286, and di...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00444a029
更新日期：1989-09-05 00:00:00
abstract：:Locked nucleic acid (LNA) is a nucleic acid analogue containing one or more LNA nucleotide monomers with a bicyclic furanose unit locked in an RNA mimicking sugar conformation. LNA oligonucleotides display unprecedented hybridization affinity toward complementary single-stranded RNA and complementary single- or double...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/bi0485732
更新日期：2004-10-26 00:00:00
abstract：:Integrin beta subunits contain a highly conserved I-like domain that is known to be important for ligand binding. Unlike integrin I domains, the I-like domain requires integrin alpha and beta subunit association for optimal folding. Pactolus is a novel gene product that is highly homologous to integrin beta subunits b...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi016047u
更新日期：2002-04-02 00:00:00
abstract：:The EphA2 receptor plays key roles in many physiological and pathological events, including cancer. The process of receptor endocytosis and the consequent degradation have attracted attention as possible means of overcoming the negative outcomes of EphA2 in cancer cells and decreasing tumor malignancy. A recent study ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300141h
更新日期：2012-03-13 00:00:00
abstract：:Phage T4 gene 32 protein (gp32) is a zinc metalloprotein which binds cooperatively and preferentially to single-stranded nucleic acids and functions as a replication and recombination accessory protein. Zn(II) coordination by gp32 employs a His-Cys3 metal ligand donor set derived from the His64-X12-Cys77-X9-Cys87-X2-C...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9617769
更新日期：1997-01-28 00:00:00
abstract：:The bovine protein tyrosine phosphatase (BPTP) is a member of the class of low-molecular weight protein tyrosine phosphatases (PTPases) found to be ubiquitous in mammalian cells. The catalytic site of BPTP contains a CX(5)R(S/T) phosphate-binding motif or P-loop (residues 12-19) which is the signature sequence for all...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi990381x
更新日期：1999-09-07 00:00:00
abstract：:Trypanosomes are purine-auxotrophic parasites that depend upon nucleoside hydrolase (NH) activity to salvage nitrogenous bases necessary for nucleic acid and cofactor synthesis. Nonspecific and purine-specific NHs have been widely studied, yet little is known about the 6-oxopurine-specific isozymes, although they are ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi100697d
更新日期：2010-10-19 00:00:00
abstract：:We have studied carbon monoxide (CO) migration and binding in the nonsymbiotic hemoglobins AHb1 and AHb2 of Arabidopsis thaliana using Fourier transform infrared (FTIR) spectroscopy combined with temperature derivative spectroscopy (TDS) at cryogenic temperatures. Both proteins have similar amino acid sequences but di...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi100768g
更新日期：2010-09-07 00:00:00
abstract：:The following polynucleotides containing the antibiotic tubercidin (Tu; 4-amino-7-beta-D-ribofuranosylpyrrolo-[2,3-d]pyrimidine) were enzymatically synthesized by polymerization of adenosine 5'-diphosphate-tubercidin 5'-diphosphate mixtures with polynucleotide phosphorylase: poly(A2,Tu), poly(A,Tu2), and poly(Tu). The...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00512a030
更新日期：1981-04-28 00:00:00
abstract：:The proposal that microtubule dynamic instability results from stabilization of microtubule ends by tubulin-GDP-Pi subunits (where Pi is inorganic phosphate) [Melki et al. (1996) Biochemistry 35, 12038] was based on studies of GTP hydrolysis and microtubule assembly that showed that tubulin-GDP-Pi subunits can transie...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972602+
更新日期：1998-09-15 00:00:00
abstract：:The NADH absorbance spectrum of nicotinoprotein (NADH-containing) alcohol dehydrogenase from Amycolatopsis methanolica has a maximum at 326 nm. Reduced enzyme-bound pyridine dinucleotide could be reversibly oxidized by acetaldehyde. The fluorescence excitation spectrum for NADH bound to the enzyme has a maximum at 325...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972115u
更新日期：1998-03-03 00:00:00
abstract：:The single reactive sulfhydryl group, located in the active site of each subunit of dimeric creatine kinase from rabbit muscle (isozyme MM), was selectively labeled with 3-(4-maleimidylphenyl)-7-(diethylamino)-4-methylcoumarin (CPM). Isozyme BB, purified to homogeneity from rabbit brain, was conjugated with the sulfhy...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00292a018
更新日期：1983-11-08 00:00:00
abstract：:Runx proteins constitute a family of mammalian transcription factors that interact with DNA through their evolutionarily conserved Runt domain. CBFbeta, alternatively denoted PEBP2beta, is the non-DNA-binding heterodimer partner and acts to enhance the DNA binding affinity of Runx proteins. Runx proteins and CBFbeta a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi010713+
更新日期：2001-09-25 00:00:00
abstract：:A study of the voltage induction of transient pores in phospholipid bilayer vesicles is reported. Unilamellar vesicles (dipalmitoylphosphatidylcholine), with a size distribution of 100 +/- 30 nm, were prepared by the method of Enoch & Strittmatter [Enoch, H., & Strittmatter, P. (1979) Proc. Natl. Acad. Sci. U.S.A. 76,...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00509a022
更新日期：1981-03-17 00:00:00
abstract：:Cold-shock proteins (Csps) are expressed at lower-than-optimum temperatures, and they function as RNA chaperones; however, no structural studies on psychrophilic Csps have been reported. Here, we aimed to investigate the structure and dynamics of the Csp of psychrophile Colwellia psychrerythraea 34H, ( Cp-Csp). Althou...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b00144
更新日期：2018-07-03 00:00:00
abstract：:Coenzyme A (CoASH) is the major low-molecular weight thiol in Staphylococcus aureus and a number of other bacteria; the crystal structure of the S. aureus coenzyme A-disulfide reductase (CoADR), which maintains the reduced intracellular state of CoASH, has recently been reported [Mallett, T.C., Wallen, J.R., Karplus, ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi062299p
更新日期：2007-03-20 00:00:00
abstract：:Membrane vesicles isolated from Escherichia coli ML 308--225 have been analyzed by crossed immunoelectrophoresis, and immunoprecipitates corresponding to the following cellular components have been identified: ATPase (EC 3.6.1,3), two or three NADH dehydrogenases (EC 1.6.99.3), D-lactate dehydrogenase (EC 1.1.1.27), g...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00575a004
更新日期：1979-04-17 00:00:00
abstract：:An endonuclease II preparation from Escherichia coli makes single strand breaks in DNA which has been treated with the carcinogen 7-bromomethyl-12-methylbenz[alpha]anthracene. In addition, the enzyme preparation excises N6-(12-methylbenz[alpha]anthracenyl-7-methyl)adenine and N2-(12-methylbenz[alpha]anthracenyl-7-met...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00697a002
更新日期：1975-12-30 00:00:00
abstract：:We have investigated the effect of increasing percentages of methylated pyrimidines on the structure of poly(dA-dU).poly(dA-dU). This was done by synthesizing analogous polynucleotides that contained deoxythymidine residues as well as deoxyuridine residues and observing their 31P NMR spectra in increasing amounts of C...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00341a033
更新日期：1985-09-24 00:00:00
abstract：:Reductive dehalogenation such as that catalyzed by iodotyrosine deiodinase (IYD) is highly unusual in aerobic organisms but necessary for iodide salvage from iodotyrosine generated during thyroxine biosynthesis. Equally unusual is the dependence of this process on flavin. Rapid kinetics have now been used to define th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00410
更新日期：2015-07-28 00:00:00
abstract：:The recruitment and organization of clathrin at endocytic sites first to form coated pits and then clathrin-coated vesicles depend on interactions between the clathrin N-terminal domain (TD) and multiple clathrin binding sequences on the cargo adaptor and accessory proteins that are concentrated at such sites. Up to f...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00065
更新日期：2015-04-28 00:00:00
abstract：:The class D β-lactamase OXA-143 has been described as an efficient penicillinase, oxacillinase, and carbapenemase. The D224A variant, known as OXA-231, was described in 2012 as exhibiting less activity toward imipenem and increased oxacillinase activity. Additionally, the P227S mutation was reported as a case of conve...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.9b00365
更新日期：2019-08-27 00:00:00
abstract：:Multisite mutagenesis of single-chain and monomeric forms of human interleukin 5 (IL-5) was performed to investigate mechanistic features of receptor activation and the possibility of differentiating sites of activation from those for receptor interaction. The normally dimeric human IL-5 contains two domains, each con...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi001467p
更新日期：2000-12-05 00:00:00
abstract：:The gene for human pancreatic cholesterol esterase consists of 11 exons and 10 introns and is 9.2 kb in length. The last and longest exon (841 nucleotides) is unique to the human gene. Functional amino acids are encoded on separate exons. The leader sequence is encoded by a single exon which carries two additional N-t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00141a017
更新日期：1992-07-07 00:00:00
abstract：:The pH-dependent behavior of the ferric forms of two soluble truncations of Rhizobium meliloti FixL, FixL (heme and kinase domains, functional), and FixLN (heme domain) are examined by UV-visible, resonance Raman, and electron paramagnetic resonance spectroscopy. Global analysis of UV-visible data indicates that the p...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi981439v
更新日期：1998-09-29 00:00:00
