Abstract:
:The following polynucleotides containing the antibiotic tubercidin (Tu; 4-amino-7-beta-D-ribofuranosylpyrrolo-[2,3-d]pyrimidine) were enzymatically synthesized by polymerization of adenosine 5'-diphosphate-tubercidin 5'-diphosphate mixtures with polynucleotide phosphorylase: poly(A2,Tu), poly(A,Tu2), and poly(Tu). The incorporation of the antibiotic was favored by the enzyme. The polymers are compared to poly(adenylic acid) [poly(A)] with respect to their structure, conformation, and ability to direct polylysine synthesis in a ribosome-dependent protein synthesis system. From physical data (thermal melting, NMR, and circular dichroism) it is concluded that tubercidin destabilizes the structure of the polynucleotide chain and that this may be due to an altered polarization of the nucleobases and their enhanced rotation around the N-glycosylic bond. Since there is an apparent correlation between thermal unfolding of the polymers and their ability to mediate polylysine synthesis, it is suggested that partial destacking of the messenger ribonucleic acid favors its binding to the ribosome and/or its ability to enhance codon-anticodon-specific protein synthesis.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Seela F,Tran TQ,Mentzel H,Erdmann VAdoi
10.1021/bi00512a030subject
Has Abstractpub_date
1981-04-28 00:00:00pages
2559-64issue
9eissn
0006-2960issn
1520-4995journal_volume
20pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::HTC cells have been labeled by short exposures to [3H]thymidine in order to identify newly synthesized DNA. By either isolating nuclei directly or isolating them after an extensive fixation with formaldehyde, we have been able to identify two phases in the maturation process of newly replicated chromatin. The first ph...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00302a036
更新日期:1984-03-27 00:00:00
abstract::Affinity-labeling experiments with 125I-endothelin derivatives using bifunctional cross-linking reagents were carried out in an attempt to identify the polypeptide component(s) of the endothelin/sarafotoxin receptors in rat brain tissues. In rat cerebellum, cortex, and caudate putamen, endothelin 1 specifically labele...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00479a012
更新日期:1990-07-10 00:00:00
abstract::We report the 1H and 15N chemical shift assignments along with an NMR-derived preliminary structure for DnaJ(1-78), a highly conserved N-terminal domain of DnaJ, the Escherichia coli Hsp40 homolog. This 9 kDa domain is believed to cooperate with DnaK, the E. coli Hsp70 homolog, in regulating a variety of cellular func...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00016a033
更新日期:1995-04-25 00:00:00
abstract::Benzoylformate decarboxylase (BFDC) and pyruvate decarboxylase (PDC) are both thiamin diphosphate-dependent enzymes. The two share a common three-dimensional structure and catalyze a similar chemical reaction, i.e., decarboxylation of 2-keto acids. However, they vary significantly in their substrate utilization patter...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9008402
更新日期:2009-09-08 00:00:00
abstract::Nitric oxide generated by bacterial nitric oxide synthase (NOS) increases the susceptibility of Gram-positive pathogens Staphylococcus aureus and Bacillus anthracis to oxidative stress, including antibiotic-induced oxidative stress. Not surprisingly, NOS inhibitors also improve the effectiveness of antimicrobials. Dev...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00431
更新日期:2015-07-07 00:00:00
abstract::Modulation of endogenous gene function, through sequence-specific recognition of double helical DNA via oligonucleotide-directed triplex formation, is a promising approach. Compared to the formation of pyrimidine motif triplexes, which require relatively low pH, purine motif appears to be the most gifted for their sta...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi1012589
更新日期:2011-05-17 00:00:00
abstract::The synthesis of sphingomyelin was studied in baby hamster kidney cells and in subcellular fractions derived from rat liver. During pulse-chase experiments with [3H]choline in tissue culture cells, the specific radioactivity of sphingomyelin continued to increase after the specific activities of phosphocholine and cyt...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00540a027
更新日期:1982-05-25 00:00:00
abstract::The interaction of bleomycin A2 with DNA has been examined by fluorescence spectroscopy and proton magnetic resonance techniques. Fluorescence bands observed at 353 and 405 nm in the spectrum of bleomycin were assigned to the bithiazole and 4-aminopyrimidine rings, respectively. Quenching of bithiazole fluorescence by...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00635a021
更新日期:1977-08-09 00:00:00
abstract::The genome of all retroviruses consists of two identical RNAs noncovalently linked near their 5' end. Dimerization of genomic RNA is thought to modulate several steps in the retroviral life cycle, such as recombination, translation, and encapsidation. The kissing-loop model of HIV-1 genome dimerization [Laughrea, M., ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi951838f
更新日期:1996-02-06 00:00:00
abstract::Despite recognition that the plasma membrane (PM) is comprised of lipid raft domains that are key organizing sites of multiple signaling pathways and other cell functions, limited information is available regarding the structure and function in sterol dynamics of these microdomains. To begin to resolve these issues, M...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0602720
更新日期:2006-10-03 00:00:00
abstract::Dihydroorotates deuteriated at both C5 and C6 have been prepared and used to probe the mechanism of the bovine liver mitochondrial dihydroorotate dehydrogenase. Primary deuterium isotope effects on kcat are observed with both (6RS)-[5(S)-2H]- and (6RS)-[6-2H] dihydroorotates (3 and 6, respectively); these effects are ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00429a041
更新日期:1989-02-07 00:00:00
abstract::The temperature dependence of the reduction potential of the CuA site in carbon monoxide inhibited cytochrome c oxidase has been measured with a spectroelectrochemical method adapted to the relatively weak near-infrared absorption of this copper ion. These measurements, together with parallel measurements on the 604-n...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00349a024
更新日期:1986-01-14 00:00:00
abstract::The kinetics of the phospholipase A2-catalyzed hydrolysis of bilayer vesicles and mixed micelles of several oxyglycero and thioglycero analogues of phospholipids have been studied. The results with vesicles show that, depending on the source of the enzyme, the rates of hydrolysis of the oxy-containing long-chain phosp...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00149a014
更新日期:1992-09-01 00:00:00
abstract::Site-directed mutagenesis has been used to construct three recombinant mutant hemoglobins (rHbs), rHb(beta L105W), rHb(alpha D94A/betaL105W), and rHb(alpha D94A). rHb(beta L105W) is designed to form a new hydrogen bond from beta 105Trp to alpha 94Asp in the alpha(1)beta(2) subunit interface to lower the oxygen binding...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001115i
更新日期:2000-11-14 00:00:00
abstract::Novel sulfated tetrasaccharide structures containing 3-O-sulfated GlcA were isolated recently from king crab cartilage chondroitin sulfate K [Sugahara, K., Tanaka, Y., Yamada, S., Seno, N., Kitagawa, H., Haslam, S. M., Morris, H. R., & Dell, A. (1996) J. Biol. Chem. 271, 26745-26754]. In this study, we prepared a seri...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi962740j
更新日期:1997-04-01 00:00:00
abstract::Transcarboxylase is made up of a central hexameric subunit (S20,W similar 12 S), three peripheral dimeric metallo subunits (S20,W similar to 5 S), and six biotinyl carboxyl carrier subunits (S20,W similar to 1.3 S). The results presented here show that the carboxyl carrier subunit is required for assembly of the 12S a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00679a010
更新日期:1975-04-22 00:00:00
abstract::Previously, we have described significant effects of human insulin on glucose metabolism in the yeast Saccharomyces cerevisiae under conditions of growth limitation. These regulations apparently rely on a transmembrane receptor capable of binding human insulin and responding by tyrosine/serine phosphorylation of a spe...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9920432
更新日期:2000-02-15 00:00:00
abstract::Fluorescence spectroscopy has been used to monitor the partitioning of a series of exchangeable neutral phospholipid probes, labeled with carbazole, indolyl or diphenylhexatrienyl moieties, between large unilamellar vesicles containing 1-palmitoyl-2-oleoylphosphatidylcholine (POPC), 1,2-dioleoyloxy-3-(trimethylammonio...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00244a018
更新日期:1991-07-30 00:00:00
abstract::The interaction between cytochrome c oxidase complex and adenosine triphosphate synthase (F1F0) complex in the purified, dispersed state and embedded in phospholipid vesicles was studied by differential scanning calorimetry and by spin-label electron paramagnetic resonance. The detergent-dispersed cytochrome oxidase a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00127a036
更新日期:1992-03-31 00:00:00
abstract::Prolyl oligopeptidase B from Galerina marginata (GmPOPB) has recently been discovered as a peptidase capable of breaking and forming peptide bonds to yield a cyclic peptide. Despite the relevance of prolyl oligopeptidases in human biology and disease, a kinetic analysis pinpointing rate-limiting steps for a member of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00012
更新日期:2017-04-18 00:00:00
abstract::The constant (CL) fragment of the immunoglobulin light chain contains only one intrachain disulfide bond buried in the interior of the molecule. The kinetics of reduction with dithiothreitol of the disulfide bond were studied at various concentrations of guanidine hydrochloride at pH 8.0 and 25 degrees C. It was found...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00356a026
更新日期:1986-04-22 00:00:00
abstract::The temperature dependence of steady-state kinetics has been studied with horse liver alcohol dehydrogenase (HLADH) using protonated and deuterated benzyl alcohol as substrates in methanol/water mixtures between +3 and -50 degrees C. Additionally, the competitive isotope effects, k(H)/k(T) and k(D)/k(T), were measured...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi002075l
更新日期:2001-02-20 00:00:00
abstract::The microtubule-associated protein tau, in a hyperphosphorylated form, aggregates into insoluble paired-helical filaments (PHFs) in Alzheimer's disease (AD) and other tauopathies. In AD, there is approximately 8 mol of phosphate per mole of tau distributed among approximately 30 PHF phosphorylation sites as compared t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi900602h
更新日期:2009-06-30 00:00:00
abstract::Base excision repair (BER) plays a vital role in maintaining genomic integrity in mammalian cells. DNA polymerase λ (Pol λ) is believed to play a backup role to DNA polymerase β (Pol β) in base excision repair. Two oxidized abasic lesions that are produced by a variety of DNA-damaging agents, including several antitum...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi301592x
更新日期:2013-02-05 00:00:00
abstract::A high degree of selectivity toward the target site of the pest organism is a desirable attribute for new safer agrochemicals. To assist in the design of novel herbicides, we determined the crystal structures of the herbicidal target enzyme 4-hydroxyphenylpyruvate dioxygenase (HPPD; EC 1.13.11.27) from the plant Arabi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049323o
更新日期:2004-08-17 00:00:00
abstract::The mechanism of the slow refolding reactions of four different pancreatic ribonucleases from ox, sheep, red deer, and roe deer has been investigated. Refolding kinetics of these proteins were very similar. In particular, a native-like intermediate, IN, was shown to be populated on the slow refolding pathway of all ri...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00336a005
更新日期:1985-07-16 00:00:00
abstract::Suberyldicholine-induced ion translocation in the millisecond time region in acetylcholine receptor rich membrane vesicles prepared from the electric organ of Electrophorus electricus was investigated in eel Ringer's solution, pH 7.0, 1 degree C. A quench-flow technique with a time resolution of 5 ms was used to measu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00294a041
更新日期:1983-12-06 00:00:00
abstract::Acidic fibroblast growth factor (aFGF) is a signal peptide-less protein that is secreted into the extracellular compartment as part of a multiprotein release complex, consisting of aFGF, S100A13 (a calcium binding protein), and a 40 kDa (p40) form of synaptotagmin (Syt1), a protein that participates in the docking of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi7002586
更新日期:2007-08-14 00:00:00
abstract::Phototropin, a blue-light photoreceptor in plants, has two FMN-binding domains named LOV1 and LOV2. We previously observed temperature-dependent FTIR spectral changes in the C=O stretching region (amide-I vibrational region of the peptide backbone) for the LOV2 domain of Adiantum phytochrome3 (phy3-LOV2), suggesting p...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061837v
更新日期:2006-12-26 00:00:00
abstract::Iron is vital for almost all organisms because of its ability to donate and accept electrons with relative ease. It serves as a cofactor for many proteins and enzymes necessary for oxygen and energy metabolism, as well as for several other essential processes. Mammalian cells utilize multiple mechanisms to acquire iro...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/bi300752r
更新日期:2012-07-24 00:00:00