Bleomycin-DNA interactions: fluorescence and proton magnetic resonance studies.

abstract：:We have examined the DNA damage produced by reaction of peroxyl radicals with human fibroblast DNA. DNA damage consisted of both strand breaks and base modifications. The extent of strand breaks and base modifications induced as a function of peroxyl radical concentration was determined by quantitation of fragment siz...

journal_title：Biochemistry

authors： Rodriguez H,Valentine MR,Holmquist GP,Akman SA,Termini J

更新日期：1999-12-14 00:00:00

abstract：:Ultrahigh-resolution X-ray diffraction data from cryo-cooled, B. lentus subtilisin crystals has been collected to a resolution of 0.78 A. The refined model coordinates have a rms deviation of 0.22 A relative to the same structure determined at room temperature and 2.0 A resolution. Several regions of main-chain and si...

journal_title：Biochemistry

authors： Kuhn P,Knapp M,Soltis SM,Ganshaw G,Thoene M,Bott R

更新日期：1998-09-29 00:00:00

abstract：:The effects of site-directed mutagenesis were used to explore the role of residues in M4 on the apparent Ki of a selective, K+-competitive inhibitor of the gastric H+,K+ ATPase, SCH28080. A double transfection expression system is described, utilizing HEK293 cells and separate plasmids encoding the alpha and beta subu...

journal_title：Biochemistry

authors： Munson KB,Lambrecht N,Sachs G

更新日期：2000-03-21 00:00:00

abstract：:Native calf thymus DNA was reacted with N-acetoxy-N-2-acetylaminofluorene (N-AcO-AAF) and its 7-fluoro and 7-iodo derivatives. Different ways of purification of the fluorene modified DNA samples were checked in order ot obtain a nucleic acid free from all noncovalently bound fluorene residues. The decrease in melting ...

journal_title：Biochemistry

authors： Fuchs RP,Lefevre JF,Pouyet J,Daune MP

更新日期：1976-07-27 00:00:00

abstract：:A number of isomeric diacylglycerophosphocholines and diacylglycero sulfates containing O-acyl and/or S-acyl ester bonds were investigated as substrates for porcine pancreatic phospholipase A2 and its zymogen. A comparison is made with the kinetic properties of the enzyme toward the corresponding glycol detergents pre...

journal_title：Biochemistry

authors： van Oort MG,Dijkman R,Hille JD,de Haas GH

更新日期：1985-12-31 00:00:00

abstract：:The pK(a) values measured previously for the internal Lys-66, Asp-66, and Glu-66 in variants of a highly stable form of staphylococcal nuclease are shifted by as many as 5 pK(a) units relative to normal pK(a) values in water. These shifts cannot be reproduced with continuum electrostatics calculations with static stru...

journal_title：Biochemistry

authors： Karp DA,Stahley MR,García-Moreno B

更新日期：2010-05-18 00:00:00

abstract：:A neutral protease has been extracted from the media of cultured metastatic tumor cells and purified approximately 1000 times after sequential ammonium sulfate fractionization, concanavalin A column chromatography, and molecular sieve chromatography. The protease has an apparent molecular weight of 70 000--80 000, is ...

journal_title：Biochemistry

authors： Liotta LA,Tryggvason K,Garbisa S,Robey PG,Abe S

更新日期：1981-01-06 00:00:00

abstract：:Virulent strains of Clostridium sordellii cause gangrenous myonecrosis in humans. The released lethal toxin (TcsL) and hemorrhagic toxin (TcsH) are regarded as the major virulence factors. TcsL inactivates low molecular weight GTP-binding proteins of the Rho/Ras subfamilies by monoglucosylation. In cultured cell lines...

journal_title：Biochemistry

authors： Schulz F,Just I,Genth H

更新日期：2009-09-29 00:00:00

abstract：:Biochemical experiments, using the well-defined human liver CYP2C9 expressed in yeast, and molecular modeling techniques were used to derive a predictive model for substrates of CYP2C9. The ability of 10 2-aroylthiophenes related to tienilic acid to act as substrates for CYP2C9 was studied. Four of them were original ...

journal_title：Biochemistry

authors： Mancy A,Broto P,Dijols S,Dansette PM,Mansuy D

更新日期：1995-08-22 00:00:00

abstract：:We have used diethyl pyrocarbonate (DEP), which carbethoxylates adenine bases, and dimethyl sulfate (DMS), which methylates guanine residues and single-stranded cytosines, to probe bases in open complexes between RNA polymerase and the lac UV5 promoter in vitro. We compared the kinetics of reactivity between bases in ...

journal_title：Biochemistry

authors： Buckle M,Buc H

更新日期：1989-05-16 00:00:00

abstract：:Glucose oxidation to CO2 was investigated in isolated perifused rat epididymal fat cells. Insulin stimulated rates of oxidation up to 30-fold. Multiple pulses of insulin or prolonged perifusion with the hormone led to a time-dependent desensitization of the cells. The action of insulin could be mimicked by H2O2. Rever...

journal_title：Biochemistry

authors： Lipkin EW,Teller DC,de Haën C

更新日期：1983-02-15 00:00:00

abstract：:Restrictocin and related fungal endoribonucleases from the α-sarcin family site-specifically cleave the sarcin/ricin loop (SRL) on the ribosome to inhibit translation and ultimately trigger cell death. Previous studies showed that the SRL folds into a bulged-G motif and tetraloop, with restrictocin achieving a specifi...

journal_title：Biochemistry

authors： Plantinga MJ,Korennykh AV,Piccirilli JA,Correll CC

更新日期：2011-04-12 00:00:00

abstract：:The interaction of the retroviral nucleocapsid protein (NC) with nucleic acids forms the basis of its varied roles in the replication cycle, which include binding and condensing the viral RNA within the virion, stimulation of the early steps in reverse transcription, and dissociation from RNA in the replication comple...

journal_title：Biochemistry

authors： Secnik J,Gelfand CA,Jentoft JE

更新日期：1992-03-24 00:00:00

abstract：:Trinucleotide repeat expansion diseases (TREDs) are correlated with elongation of CNG DNA and RNA repeats to pathological level. This paper shows, for the first time, complete data concerning thermodynamic stabilities of RNA with CNG trinucleotide repeats. Our studies include the stability of oligoribonucleotides comp...

journal_title：Biochemistry

authors： Broda M,Kierzek E,Gdaniec Z,Kulinski T,Kierzek R

更新日期：2005-08-16 00:00:00

abstract：:We have examined the effects on redox kinetics of changing the reduction potential of the mu-oxo-bridged binuclear iron center in octameric hemerythrin (Hr) from Phascolopsis gouldii. The opportunity to examine such effects is provided by the availability of mu-sulfidomethemerythrin (mu-S2-metHr), whose [Fe(III),Fe(II...

journal_title：Biochemistry

authors： Pearce LL,Utecht RE,Kurtz DM Jr

更新日期：1987-12-29 00:00:00

abstract：:One of the most promising approaches to anti-hepatitis C virus drug discovery is the development of inhibitors of the virally encoded protease NS3. This chymotrypsin-like serine protease is essential for the maturation of the viral polyprotein, and processing requires complex formation between NS3 and its cofactor NS4...

journal_title：Biochemistry

authors： Bianchi E,Orrù S,Dal Piaz F,Ingenito R,Casbarra A,Biasiol G,Koch U,Pucci P,Pessi A

更新日期：1999-10-19 00:00:00

abstract：:A complex of photoreceptor phoborhodopsin (ppR; also called sensory rhodopsin II) and its cognate halobacterial transducer II (pHtrII) existing in the plasma membrane mediates the light signal to the cytoplasm in the earliest step of negative phototaxis in Natronomonas pharaonis. We have investigated the dynamics of t...

journal_title：Biochemistry

authors： Taniguchi Y,Ikehara T,Kamo N,Yamasaki H,Toyoshima Y

更新日期：2007-05-08 00:00:00

abstract：:We have used a site-specific approach to investigate the mutagenic potential of (+)- and (-)-trans-anti-benzo[a]pyrene diol epoxide (BPDE) DNA adducts. Oligodeoxyribonucleotides (5'TCCTCCTG1G2-CCTCTC), modified at the exocyclic amino groups of G1 or G2, were incorporated into a single-stranded shuttle vector and intro...

journal_title：Biochemistry

authors： Moriya M,Spiegel S,Fernandes A,Amin S,Liu T,Geacintov N,Grollman AP

更新日期：1996-12-24 00:00:00

abstract：:An endogenous calcium-activated sulfhydryl protease in chick oviduct cytosol has been utilized to study the structure of the chick oviduct progesterone receptor subunits, progestophilins A (79 000 g/mol) and B (117 000 g/mol). The protease is not a normal component of the native progesterone receptor aggregate (6 and ...

journal_title：Biochemistry

authors： Vedeckis WV,Schrader WT,O'Malley BW

更新日期：1980-01-22 00:00:00

abstract：:The eukaryotic transcriptional factor TFIIS enhances transcript elongation by RNA polymerase II. Here we describe two functional domains in the 280 amino acid human TFIIS protein: residues within positions 100-230 are required for binding to polymerase, and residues 230-280, which form a zinc finger, are required in c...

journal_title：Biochemistry

authors： Agarwal K,Baek KH,Jeon CJ,Miyamoto K,Ueno A,Yoon HS

更新日期：1991-08-06 00:00:00

abstract：:Bacteriophage T7 codes for a single-stranded DNA binding protein. This protein is the product of gene 2.5 and has been found previously to stimulate specifically the activity of the phage-coded DNA polymerase. We report here that the T7 DNA binding protein also stimulates the activity of the phage-coded exonuclease. T...

journal_title：Biochemistry

authors： Roberts L,Sadowski P,Wong JT

更新日期：1982-11-09 00:00:00

abstract：:The HIV-1 fusion peptide serves as a useful model system for understanding viral/target cell fusion, at least to the lipid-mixing stage. Previous solid-state NMR studies have shown that the membrane-bound HIV-1 fusion peptide adopts an extended conformation in a lipid mixture close to that of host cells of the virus. ...

journal_title：Biochemistry

authors： Yang J,Weliky DP

更新日期：2003-10-14 00:00:00

abstract：:Eukaryotic initiation factor 5 (eIF5) plays multiple roles in translation initiation. Its N-terminal domain functions as a GTPase-activator protein (GAP) for GTP bound to eIF2, while its C-terminal region nucleates the interactions between multiple translation factors, including eIF1, which acts to inhibit GTP hydroly...

journal_title：Biochemistry

authors： Conte MR,Kelly G,Babon J,Sanfelice D,Youell J,Smerdon SJ,Proud CG

更新日期：2006-04-11 00:00:00

abstract：:The unfolding of human plasma alpha 1-acid glycoprotein (AGP) induced by heat or guanidine hydrochloride was studied under equilibrium conditions. In thermal unfolding, an intermediate state was detected by the appearance of unusual positive difference absorption bands in the 287-295-nm region, which occurred at lower...

journal_title：Biochemistry

authors： Rojo-Domínguez A,Zubillaga-Luna R,Hernández-Arana A

更新日期：1990-09-18 00:00:00

abstract：:Despite a number of studies, the formation of the Michaelis complexes between ferredoxin-NADP (+) reductases and NADP(H) eluded detailed investigations by rapid kinetic techniques because of their high formation rates. Moreover, the reversible nature of the reaction of hydride ion transfer between these enzymes and NA...

journal_title：Biochemistry

authors： Pennati A,Zanetti G,Aliverti A,Gadda G

更新日期：2008-03-18 00:00:00

abstract：:Twenty-five different temperature-sensitive point mutations at 20 sites in the lysozyme gene of bacteriophage T4 have been identified. All of the mutations alter amino acid side chains that have lower than average crystallographic thermal factors and reduced solvent accessibility in the folded protein. This suggests t...

journal_title：Biochemistry

authors： Alber T,Sun DP,Nye JA,Muchmore DC,Matthews BW

更新日期：1987-06-30 00:00:00

abstract：:An inhibitor of the insulin receptor tyrosine kinase (IRTK), (hydroxy-2-naphthalenyl-methyl) phosphonic acid, was designed and synthesized and was shown to be an inhibitor of the biological effects of insulin in vitro. With a wheat germ purified human placental insulin receptor preparation, this compound inhibited the...

journal_title：Biochemistry

authors： Saperstein R,Vicario PP,Strout HV,Brady E,Slater EE,Greenlee WJ,Ondeyka DL,Patchett AA,Hangauer DG

更新日期：1989-06-27 00:00:00

abstract：:The Escherichia coli Ada protein repairs methylphosphotriesters in DNA through direct, irreversible transfer to a cysteine residue on the protein, Cys 69. Methylation of Cys 69 increases the sequence-specific DNA-binding activity of Ada by 10(3)-fold, enabling the methylated protein to activate transcription of a meth...

journal_title：Biochemistry

authors： Sun LJ,Yim CK,Verdine GL

更新日期：2001-09-25 00:00:00

abstract：:The left-handed polyproline II (P(II)) helix is a structure that has been given a great deal of attention lately because of its role in a wide variety of physiologically important processes and potential significance in protein unfolded states. Recent work by several authors has shown that residues besides proline can...

journal_title：Biochemistry

authors： Chellgren BW,Creamer TP

更新日期：2004-05-18 00:00:00

abstract：:Biochemical and immunochemical data from the present investigation reveal the existence of a p85/p110 phosphoinositide 3-kinase (PI 3-kinase) in rat liver nuclei. 32P-Labeling of membrane phosphoinositides by incubating intact nuclei with [gamma-32P]ATP results in the formation of [32P]phosphatidyl-inositol 3,4, 5-tri...

journal_title：Biochemistry

authors： Lu PJ,Hsu AL,Wang DS,Yan HY,Yin HL,Chen CS

更新日期：1998-04-21 00:00:00