Native-like folding intermediates of homologous ribonucleases.

abstract：:To identify the elements which regulate the liver transcription of the human type II phospholipase A2 gene and its stimulation by interleukin 6, the 5' flanking region from -1614 to +806 and several 3' and 5' deleted fragments have been analyzed in CAT assays. Negative regulatory elements have been located in the regi...

journal_title：Biochemistry

authors： Olivier JL,Fan Q,Salvat C,Ziari M,Kong L,Mangeney M,Bereziat G

更新日期：1994-06-14 00:00:00

abstract：:We synthesized the 26-residue deoxynucleotide sequence d(TTCCT5GGAATTCCT5GGAA) which folds intramolecularly to form a dumbbell-shaped, double-hairpin structure with a gap between the 3' and the 5' ends. We used T4 polynucleotide kinase to phosphorylate the 5' end followed by T4 DNA ligase to close the 3' and 5' ends. ...

journal_title：Biochemistry

authors： Erie DA,Jones RA,Olson WK,Sinha NK,Breslauer KJ

更新日期：1989-01-10 00:00:00

abstract：:A time-resolved microscope fluorimeter based on a synchroscan streak camera and a fast pulsed laser system has been developed to measure the fluorescence lifetime decay under the fluorescence microscope. This system allows one to measure the nanosecond fluorescence lifetimes of fluorophores in a small spot (0.8-6.3 mi...

journal_title：Biochemistry

authors： Kusumi A,Tsuji A,Murata M,Sako Y,Yoshizawa AC,Kagiwada S,Hayakawa T,Ohnishi S

更新日期：1991-07-02 00:00:00

abstract：:The fluorescence spectrum of C1 inhibitor (C1-Inh) in aqueous buffer has a maximum at 324 nm which shifts to 358 nm in 6.0 M guanidinium chloride (GdmC1), indicating that fluorescent tryptophans are buried in the native protein. When titrated with GdmC1, the fluorescence intensity, polarization, and emission maximum o...

journal_title：Biochemistry

authors： Lennick M,Brew SA,Ingham KC

更新日期：1985-05-07 00:00:00

abstract：:A crystal structure of human fibrinogen has been determined at approximately 3.3 A resolution. The protein was purified from human blood plasma, first by a cold ethanol precipitation procedure and then by stepwise chromatography on DEAE-cellulose. A product was obtained that was homogeneous on SDS-polyacrylamide gels....

journal_title：Biochemistry

authors： Kollman JM,Pandi L,Sawaya MR,Riley M,Doolittle RF

更新日期：2009-05-12 00:00:00

abstract：:Conjugative transfer of F-like plasmids in Escherichia coli is repressed by a plasmid-encoded protein, FinO. FinO blocks the translation of TraJ, a positive activator of transcription of genes required for conjugation. FinO binds a traJ antisense RNA, FinP, thereby protecting it from degradation, and catalyzes FinP-tr...

journal_title：Biochemistry

authors： Ghetu AF,Gubbins MJ,Oikawa K,Kay CM,Frost LS,Glover JN

更新日期：1999-10-19 00:00:00

abstract：:HK31 (S565-K595) has previously been shown to encompass the binding domain for plasma prekallikrein (PK) within domain 6 of high molecular weight kininogen (HK). The complementary binding domain for HK within PK is mapped to PK56 (F56-G86), in the apple 1 domain, and to PK266 (K266-C295), in the apple 4 domain. Isothe...

journal_title：Biochemistry

authors： Lin Y,Shenoy SS,Harris RB,Colman RW

更新日期：1996-10-01 00:00:00

abstract：:Although the terms "excessive reactive oxygen species (ROS)" and "oxidative stress" are widely used, the implications of oxidative stress are often misunderstood. ROS are not a single species but a variety of compounds, each with unique biochemical properties and abilities to react with biomolecules. ROS cause activat...

journal_title：Biochemistry

authors： AbdulSalam SF,Thowfeik FS,Merino EJ

更新日期：2016-09-27 00:00:00

abstract：:Proton-detected heteronuclear multiple-bond 1H-13C correlations (HMBC) previously have been used for assignment purposes in a variety of isotopically enriched proteins. In the present study it is demonstrated that the technique yields an almost complete assignment of the natural abundance 13C spectrum of the protein b...

journal_title：Biochemistry

authors： Hansen PE

更新日期：1991-10-29 00:00:00

abstract：:The crystal structure of the heme-copper oxidases suggested a putative channel of oxygen entry into the heme-copper site of O(2) reduction. Changing a conserved valine near this center in cytochrome bo(3) of Escherichia coli to isoleucine caused a significant increase in the apparent K(M) for oxygen with little or no ...

journal_title：Biochemistry

authors： Riistama S,Puustinen A,Verkhovsky MI,Morgan JE,Wikström M

更新日期：2000-05-30 00:00:00

abstract：:ATP hydrolysis by the maltose transporter (MalFGK(2)) is regulated by maltose binding protein (MBP). Binding of maltose to MBP brings about a conformational change from open to closed that leads to a strong stimulation of the MalFGK(2) ATPase. In this study, we address the long-standing but enigmatic observation that ...

journal_title：Biochemistry

authors： Gould AD,Telmer PG,Shilton BH

更新日期：2009-08-25 00:00:00

abstract：:The structures of two RNA duplexes, whose sequences correspond to portions of the ColE1 plasmid copy control RNA I and RNA II, have been determined. Crystals containing the 18mers 5'-CA CCGUUGGUAGCGGUGC-3' and 5'-CACCGCUACCAACGGUGC-3' diffract to 1.20 A resolution while those containing the 19mers 5'-GCACCGUUGGUAGCGGU...

journal_title：Biochemistry

authors： Klosterman PS,Shah SA,Steitz TA

更新日期：1999-11-09 00:00:00

abstract：:Addition of short sequences of dCMP residues to the 3'-OH end of duplex linear DNAs allows rapid and efficient transcription to be initiated at these sites by purified mammalian RNA polymerase II [Kadesch, T. R., & Chamberlin, M. J. (1982) J. Biol. Chem. 257, 5286-5295]. The use of such tailed DNA templates should all...

journal_title：Biochemistry

authors： Dedrick RL,Chamberlin MJ

更新日期：1985-04-23 00:00:00

abstract：:The role of basic residues of the 70-80-loop, Arg(74), Arg(75), and Lys(78) (chymotrypsin numbering) in the catalytic function of activated protein C (APC) was investigated by expressing mutants of protein C in which these residues were replaced with Ala in three separate constructs. Following purification to homogene...

journal_title：Biochemistry

authors： Yang L,Manithody C,Rezaie AR

更新日期：2002-05-14 00:00:00

abstract：:The interaction of the retroviral nucleocapsid protein (NC) with nucleic acids forms the basis of its varied roles in the replication cycle, which include binding and condensing the viral RNA within the virion, stimulation of the early steps in reverse transcription, and dissociation from RNA in the replication comple...

journal_title：Biochemistry

authors： Secnik J,Gelfand CA,Jentoft JE

更新日期：1992-03-24 00:00:00

abstract：:Changes in pH can alter the structure and activity of proteins and may be used by the cell to control molecular function. This coupling can also be used in non-native applications through the design of pH-sensitive biomolecules. For example, the pH (low) insertion peptide (pHLIP) can spontaneously insert into a lipid ...

journal_title：Biochemistry

authors： Ng DP,Deber CM

更新日期：2016-08-09 00:00:00

abstract：:The activation of adenosine-5'-monophosphate aminohydrolase from rabbit skeletal muscle by H+ has been demonstrated. Evidence is presented which indicates that the binding of H+ and K+ is linked, in that the dissociation constant (KA) for K+ activation is reduced as the pH is lowered. Concomitantly, the pK of several ...

journal_title：Biochemistry

authors： Campbell JC,Suelter CH

更新日期：1977-11-01 00:00:00

abstract：:While the insertion of a hydrophobic peptide or membrane protein segment into the bilayer can be spontaneous and driven mainly by the hydrophobic effect, anionic lipids, which comprise ca. 20% of biological membranes, provide a source of electrostatic attractions for binding of proteins/peptides into membranes. To unr...

journal_title：Biochemistry

authors： Liu LP,Deber CM

更新日期：1997-05-06 00:00:00

abstract：:A procedure for universal 13C and/or 15N labeling of microbial peptides which are produced by fermentation in complex media and its application to two food-preserving bacteriocins from lactic acid bacteria are described. Isotopic enrichment of nisin A (from Lactococcus lactis) and of leucocin A (from Leuconostoc gelid...

journal_title：Biochemistry

authors： Sailer M,Helms GL,Henkel T,Niemczura WP,Stiles ME,Vederas JC

更新日期：1993-01-12 00:00:00

abstract：:Safe and effective algaecides are needed to control agriculturally and environmentally significant algal species. Four series (6, 10, 17, and 21) of 29 novel 4-aminopyrimidine derivatives were rationally designed and synthesized. A part of 10, 17, and 21 displayed potent inhibition of Escherichia coli pyruvate dehydro...

journal_title：Biochemistry

authors： Zhou Y,Feng J,He H,Hou L,Jiang W,Xie D,Feng L,Cai M,Peng H

更新日期：2017-12-12 00:00:00

abstract：:We have examined the reduction of cyanide by using the purified component proteins of nitrogenase (Av1 and Av2). The previously reported self-inhibition phenomenon was found to be an artifact. One of the two species present in cyanide solutions, CN-, was shown to be a potent reversible inhibitor (Ki = 27 microM) of to...

journal_title：Biochemistry

authors： Li J,Burgess BK,Corbin JL

更新日期：1982-08-31 00:00:00

abstract：:The polysaccharide-rich cell walls (CWs) of plants perform essential functions such as maintaining tensile strength and allowing plant growth. Using two- and three-dimensional magic-angle-spinning (MAS) solid-state NMR and uniformly (13)C-labeled Arabidopsis thaliana, we have assigned the resonances of the major polys...

journal_title：Biochemistry

authors： Dick-Pérez M,Zhang Y,Hayes J,Salazar A,Zabotina OA,Hong M

更新日期：2011-02-15 00:00:00

abstract：:Escherichia coli DNA photolyase contains a stable flavin radical that is readily photoreduced in the presence of added electron donors. Picosecond, nanosecond, and conventional flash photolysis technique have been employed to investigate the events leading to photoreduction from 40 ps to tens of milliseconds following...

journal_title：Biochemistry

authors： Heelis PF,Okamura T,Sancar A

更新日期：1990-06-19 00:00:00

abstract：:Ultraviolet (uv)-light-mediated formation of protein-DNA adducts in Chinese hamster cell chromatin was investigated in an attempt to compare chromatin alterations induced in vitro with those observed in vivo. Three independent methods of analysis indicated stable protein-DNA associations: (1) a membrane filter assay w...

journal_title：Biochemistry

authors： Strniste GF,Rall SC

更新日期：1976-04-20 00:00:00

abstract：:Polyclonal antibody to mitochondrial P-450c27/25 reacted with two proteins of apparent molecular masses of 52 kilodaltons (kDa) and 50 kDa from the female rat liver mitochondrial proteins bound to an omega-octylaminoagarose column. The two proteins were purified to greater than 85% homogeneity by DEAE-Sephacel and hyd...

journal_title：Biochemistry

authors： Addya S,Zheng YM,Shayiq RM,Fan JY,Avadhani NG

更新日期：1991-08-27 00:00:00

abstract：:Tau is a microtubule associated protein implicated in the pathogenesis of several neurodegenerative diseases. Because of the channel forming properties of other amyloid peptides, we employed planar lipid bilayers and atomic force microscopy to test tau for its ability to form ion permeable channels. Our results demons...

journal_title：Biochemistry

authors： Patel N,Ramachandran S,Azimov R,Kagan BL,Lal R

更新日期：2015-12-22 00:00:00

abstract：:A site-specific analog of ATP, 6,6'-dithiobis (inosinyl imidodiphosphate (S2P-PNP), inactivates the ATPase activities of myosin's proteolytic fragments, heavy meromyosin (HMM) and subfragment one (SF1), by formation of mixed disulfides between the 6 position of the purine ring and certain key cysteines. The stoichiome...

journal_title：Biochemistry

authors： Wagner PD,Yount RG

更新日期：1975-05-06 00:00:00

abstract：:The backbone dynamics of uniformly 15N-labeled interleukin-1 beta are investigated by using two-dimensional inverse detected heteronuclear 15N-1H NMR spectroscopy. 15N T1, T2, and NOE data at a spectrometer frequency of 600 MHz are obtained for 90% of the backbone amide groups. The data provide evidence for motions on...

journal_title：Biochemistry

authors： Clore GM,Driscoll PC,Wingfield PT,Gronenborn AM

更新日期：1990-08-14 00:00:00

abstract：:The ferritin IRE, a highly conserved (96-99% in vertebrates) mRNA translation regulatory element in animal mRNA, was studied by molecular modeling (using MC-SYM and DOCKING) and by NMR spectroscopy. Cobalt(III) hexammine was used to model hydrated Mg2+. IRE isoforms in other mRNAs regulate mRNA translation or stabilit...

journal_title：Biochemistry

authors： Gdaniec Z,Sierzputowska-Gracz H,Theil EC

更新日期：1998-02-10 00:00:00

abstract：:Attenuated total reflection Fourier transform infrared (ATR-FTIR) difference spectroscopy has been performed on samples of bovine cytochrome c oxidase that have been deposited as a thin film on the surface of a silicon microprism. The technique has several advantages over transmission methods in terms of amount of mat...

journal_title：Biochemistry

authors： Rich PR,Breton J

更新日期：2002-01-22 00:00:00