Abstract:
:Attenuated total reflection Fourier transform infrared (ATR-FTIR) difference spectroscopy has been performed on samples of bovine cytochrome c oxidase that have been deposited as a thin film on the surface of a silicon microprism. The technique has several advantages over transmission methods in terms of amount of material required, the time required to reach sufficient optical stability, and the range of reactants that can be repetitively added and removed. The ATR-FTIR method has been used to record redox difference spectra of cytochrome c oxidase in the unligated and cyanide-ligated states. By subtraction of the spectra, the redox FTIR difference spectrum of heme a(3) can be resolved from those of the other metal centers. This difference spectrum is compared with available vibrational and Raman data on homologous oxidases and on heme A model compounds.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Rich PR,Breton Jdoi
10.1021/bi0109717subject
Has Abstractpub_date
2002-01-22 00:00:00pages
967-73issue
3eissn
0006-2960issn
1520-4995pii
bi0109717journal_volume
41pub_type
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