Preferential location of bulged guanosine internal to a G.C tract by 1H NMR.

abstract：:Structural and genetic studies indicate that the antibacterial compound triclosan, an additive in many personal care products, is an inhibitor of EnvM, the enoyl reductase from Escherichia coli. Here we show that triclosan specifically inhibits InhA, the enoyl reductase from Mycobacterium tuberculosis and a target for...

journal_title：Biochemistry

authors： Parikh SL,Xiao G,Tonge PJ

更新日期：2000-07-04 00:00:00

abstract：:Microtubules, ordinarily cold-labile structures, are made entirely resistant to cold temperature by the presence of substoichiometric amounts of STOP (stable tubule only polypeptide), a microtubule-associated protein. We have produced a monoclonal antibody which specifically recognizes a 145-kDa protein previously imp...

journal_title：Biochemistry

authors： Pirollet F,Rauch CT,Job D,Margolis RL

更新日期：1989-01-24 00:00:00

abstract：:A new type of thrombin exo-site inhibitor has been designed with enhanced inhibitory potency and increased metabolic stability. With the aid of the model of the structure of the thrombin-hirudin fragment complex [Yue, S.-Y., DiMaio, J., Szewczuk, Z., Purisima, E. O., Ni, F., & Konishi, Y. (1992) Protein Eng. 5, 77-85]...

journal_title：Biochemistry

authors： Szewczuk Z,Gibbs BF,Yue SY,Purisima EO,Konishi Y

更新日期：1992-09-29 00:00:00

abstract：:We have synthesized Deltalac-acetogenins that are new acetogenin mimics possessing two n-alkyl tails without an alpha,beta-unsaturated gamma-lactone ring and suggested that their inhibition mechanism may be different from that of common acetogenins [Hamada et al. (2004) Biochemistry 43, 3651-3658]. To elucidate the in...

journal_title：Biochemistry

authors： Ichimaru N,Murai M,Abe M,Hamada T,Yamada Y,Makino S,Nishioka T,Makabe H,Makino A,Kobayashi T,Miyoshi H

更新日期：2005-01-18 00:00:00

abstract：:A cell-free system for synthesizing protein from wheat germ was used to translate the messenger RNA extracted from 16-day embryonic chick calvaria. A part of the product had properties similar to collagenous peptides and served as a substrate for prolyl hydroxylase, an enzyme specific for collagen. The level of potass...

journal_title：Biochemistry

authors： Benveniste K,Wilczek J,Ruggieri A,Stern R

更新日期：1976-02-24 00:00:00

abstract：:In order to evaluate the contributions that histidine residues might play both in the catalytic activities of endonuclease V and in binding to nontarget DNA, the technique of oligonucleotide site directed mutagenesis was used to create mutations at each of the four histidine residues in the endonuclease V gene. Althou...

journal_title：Biochemistry

authors： Augustine ML,Hamilton RW,Dodson ML,Lloyd RS

更新日期：1991-08-13 00:00:00

abstract：:The origin of the "S3" EPR signal from calcium-depleted photosystem 2 samples has been investigated. This signal is observed after freezing samples under illumination and has been assigned to an interaction between the manganese cluster and an oxidized histidine radical [Boussac et al. (1990) Nature 347; 303-306]. In ...

journal_title：Biochemistry

authors： Hallahan BJ,Nugent JH,Warden JT,Evans MC

更新日期：1992-05-19 00:00:00

abstract：:Classical strategies for structure analysis of proteins interacting with a lipid phase typically correlate ensemble secondary structure content measurements with changes in the spectroscopic responses of localized aromatic residues or reporter molecules to map regional solvent environments. Deep-UV resonance Raman (DU...

journal_title：Biochemistry

authors： Halsey CM,Oshokoya OO,Jiji RD,Cooley JW

更新日期：2011-08-02 00:00:00

abstract：:c-Myc is a member of a family of sequence specific-DNA binding proteins that are thought to regulate the transcription of genes involved in normal cell growth, differentiation, and apoptosis. In order to understand how human c-myc functions as a transcription factor, we have studied the mechanism of action and structu...

journal_title：Biochemistry

authors： McEwan IJ,Dahlman-Wright K,Ford J,Wright AP

更新日期：1996-07-23 00:00:00

abstract：:It is well-known, but little understood, that the nucleotide sequences between phased A(4-6)-tracts (at 10-11 bp intervals) have only a slight effect on overall curvature. To explore this phenomenon, we have examined the gel-migration properties of sequences containing both A-tracts as well as G-tracts (i.e., sequence...

journal_title：Biochemistry

authors： Merling A,Sagaydakova N,Haran TE

更新日期：2003-05-06 00:00:00

abstract：:The membrane-bound beta-subunit of oxaloacetate decarboxylase from Klebsiella pneumoniae catalyzes the decarboxylation of carboxybiotin, which is coupled to Na(+) translocation and consumes a periplasmically derived proton. Upon site-directed mutagenesis of 20 polar and/or conserved residues within putative membrane-i...

journal_title：Biochemistry

authors： Jockel P,Schmid M,Choinowski T,Dimroth P

更新日期：2000-04-18 00:00:00

abstract：:The effect of protonation events on the charge equilibrium between tyrosine-D and the water-oxidizing complex in photosystem II has been studied by time-resolved measurements of the EPR signal IIslow at room temperature. The flash-induced oxidation of YD by the water-oxidizing complex in the S2 state is a monophasic p...

journal_title：Biochemistry

authors： Vass I,Styring S

更新日期：1991-01-22 00:00:00

abstract：:Cryptophycin-52 (LY355703) is currently undergoing clinical evaluation for cancer chemotherapy. It is a potent suppressor of microtubule dynamics in vitro, and low picomolar concentrations appear to inhibit cancer cell proliferation at mitosis by stabilizing spindle microtubules. In the present study, using [(3)H]cryp...

journal_title：Biochemistry

authors： Panda D,Ananthnarayan V,Larson G,Shih C,Jordan MA,Wilson L

更新日期：2000-11-21 00:00:00

abstract：:The solution conformation of an agalactosyl penta-antennary "bisected" N-linked glycan from hen ovomucoid has been determined using a combination of 1H-NMR NOE measurements and restrained molecular dynamics (MD) simulations. The majority of glycosidic linkages exhibited restricted torsional fluctuations about the glob...

journal_title：Biochemistry

authors： Rutherford TJ,Neville DC,Homans SW

更新日期：1995-10-31 00:00:00

abstract：:In an attempt to understand the multifunctional involvement of beta(2)-glycoprotein I (beta(2)GPI) in autoimmune diseases, thrombosis, atherosclerosis, and inflammatory processes, substantial interest is focused on the interaction of beta(2)GPI with negatively charged ligands, in particular, with acidic phospholipids....

journal_title：Biochemistry

authors： Hammel M,Schwarzenbacher R,Gries A,Kostner GM,Laggner P,Prassl R

更新日期：2001-11-27 00:00:00

abstract：:Site-directed mutagenesis has been used to construct three recombinant mutant hemoglobins (rHbs), rHb(beta L105W), rHb(alpha D94A/betaL105W), and rHb(alpha D94A). rHb(beta L105W) is designed to form a new hydrogen bond from beta 105Trp to alpha 94Asp in the alpha(1)beta(2) subunit interface to lower the oxygen binding...

journal_title：Biochemistry

authors： Fang TY,Simplaceanu V,Tsai CH,Ho NT,Ho C

更新日期：2000-11-14 00:00:00

abstract：:We have examined in detail the kinetics of binding of the serpin alpha 2-antiplasmin to the serine proteases alpha-chymotrypsin and plasmin. These represent model systems for serpin binding. We find, in contrast to earlier published results with alpha 2-antiplasmin and plasmin, that binding is reversible, and slow bin...

journal_title：Biochemistry

authors： Longstaff C,Gaffney PJ

更新日期：1991-01-29 00:00:00

abstract：:Endogenous protein kinase inhibitors are essential for a wide range of physiological functions. These endogenous inhibitors may mimic peptide substrates as in the case of the heat-stable protein kinase inhibitor (PKI), or they may mimic nucleotide triphosphates. Natural product inhibitors, endogenous to the unique org...

journal_title：Biochemistry

authors： Narayana N,Diller TC,Koide K,Bunnage ME,Nicolaou KC,Brunton LL,Xuong NH,Ten Eyck LF,Taylor SS

更新日期：1999-02-23 00:00:00

abstract：:Currently, the relatively high cost of enzymes such as glycoside hydrolases that catalyze cellulose hydrolysis represents a barrier to commercialization of a biorefinery capable of producing renewable transportable fuels such as ethanol from abundant lignocellulosic biomass. Among the many families of glycoside hydrol...

journal_title：Biochemistry

authors： Harris PV,Welner D,McFarland KC,Re E,Navarro Poulsen JC,Brown K,Salbo R,Ding H,Vlasenko E,Merino S,Xu F,Cherry J,Larsen S,Lo Leggio L

更新日期：2010-04-20 00:00:00

abstract：:Oxalate:ferredoxin oxidoreductase (OOR) is an unusual member of the thiamine pyrophosphate (TPP)-dependent 2-oxoacid:ferredoxin oxidoreductase (OFOR) family in that it catalyzes the coenzyme A (CoA)-independent conversion of oxalate into 2 equivalents of carbon dioxide. This reaction is surprising because binding of C...

journal_title：Biochemistry

authors： Pierce E,Mansoorabadi SO,Can M,Reed GH,Ragsdale SW

更新日期：2017-06-06 00:00:00

abstract：:A stereochemical analysis has been carried out on two vinylpyruvate hydratases (VPH), which convert 2-hydroxy-2,4-pentadienoate to 2-keto-4S-hydroxypentanoate in meta-fission pathways. Bacterial strains with this pathway can use aromatic compounds as sole sources of energy and carbon. The analysis was carried out usin...

journal_title：Biochemistry

authors： Johnson WH Jr,Stack TM,Taylor SM,Burks EA,Whitman CP

更新日期：2016-07-26 00:00:00

abstract：:Phosphorylated proteins may play an important role in regulating the metabolism or function of rod photoreceptors. In mammalian retinas, a photoreceptor protein of 33 000 (33K) molecular weight is phosphorylated in a cyclic nucleotide dependent manner in vitro. Since light initiates the activation of a photoreceptor-s...

journal_title：Biochemistry

authors： Lee RH,Brown BM,Lolley RN

更新日期：1984-04-24 00:00:00

abstract：:RalA and RalB are members of the Ras family of small G proteins and are activated downstream of Ras via RalGEFs. The RalGEF-Ral axis represents one of the major effector pathways controlled by Ras and as such is an important pharmacological target. RalA and RalB are approximately 80% identical at the amino acid level;...

journal_title：Biochemistry

authors： Campbell LJ,Peppa M,Crabtree MD,Shafiq A,McGough NF,Mott HR,Owen D

更新日期：2015-02-17 00:00:00

abstract：:A set of peptides derived from the N-terminal domain of the ribosomal protein L9 (NTL9) have been characterized in an effort to define the minimum unit of this domain required to fold and to provide model peptides for the analysis of electrostatic interactions in the unfolded state. NTL9 is a 56-residue alpha-beta pro...

journal_title：Biochemistry

authors： Horng JC,Moroz V,Rigotti DJ,Fairman R,Raleigh DP

更新日期：2002-11-12 00:00:00

abstract：:Inherently curved DNA segments, associated with short runs of adenines, have been identified in many gene regulatory regions, yet their physiological significance remains unknown. The observations reported in this study indicate that intrinsically bent nucleic acid fragments are characterized by substantially attenuat...

journal_title：Biochemistry

authors： Shatzky-Schwartz M,Hiller Y,Reich Z,Ghirlando R,Weinberger S,Minsky A

更新日期：1992-03-03 00:00:00

abstract：:Vangl1 and Vangl2 are membrane proteins that play an important role in neurogenesis, and Vangl1/Vangl2 mutations cause neural tube defects in mice and humans. At the cellular level, Vangl proteins regulate the establishment of planar cell polarity (PCP), a process requiring membrane assembly of asymmetrically distribu...

journal_title：Biochemistry

authors： Iliescu A,Gravel M,Horth C,Apuzzo S,Gros P

更新日期：2011-03-29 00:00:00

abstract：:Glutamate mutase is one of a group of adenosylcobalamin-dependent enzymes that catalyze unusual isomerizations that proceed through organic radical intermediates generated by homolytic fission of the coenzyme's unique cobalt-carbon bond. These enzymes are part of a larger family of enzymes that catalyze radical chemis...

journal_title：Biochemistry

authors： Cheng MC,Marsh EN

更新日期：2007-01-23 00:00:00

abstract：:Phosphorescence from the lone tryptophan residue has been studied to monitor the interaction of myelin basic protein with phosphatidylserine vesicles. Spectral shifts in the phosphorescence of the protein in a glycerol-buffer (70:30 w/w) solvent at low temperature are consistent with fluorescence data obtained under a...

journal_title：Biochemistry

authors： Vadas EB,Melançon P,Braun PE,Galley WC

更新日期：1981-05-26 00:00:00

abstract：:In iron-deficient conditions, Azomonas macrocytogenes ATCC 12334 excretes a fluorescent siderophore called azoverdin, which is composed of a six-amino-acid peptide chain linked to a chromophore. Azoverdin chelates iron(III) very strongly, solubilizing it and transporting it back into the cells using an outer-membrane ...

journal_title：Biochemistry

authors： Wasielewski E,Atkinson RA,Abdallah MA,Kieffer B

更新日期：2002-10-15 00:00:00

abstract：:Type I protein arginine methyltransferases (PRMTs) catalyze asymmetric dimethylation of various proteins, and their dysregulations often correlate with tumorigenesis or developmental deficiency. Recent studies have focused on the in vivo substrate identification and the enzyme mechanism with peptide substrates. Howeve...

journal_title：Biochemistry

authors： Lee WC,Lin WL,Matsui T,Chen ES,Wei TY,Lin WH,Hu H,Zheng YG,Tsai MD,Ho MC

更新日期：2015-12-29 00:00:00