Mechanism of the interaction of beta(2)-glycoprotein I with negatively charged phospholipid membranes.

abstract：:The role of interleukin-8 (IL-8) and related CXC chemokines has been demonstrated in many human diseases. However, more profound studies, e.g., by blocking the effect of these inflammatory mediators, request animal models and hence the identification of all human counterparts for commonly used laboratory animals. In t...

journal_title：Biochemistry

authors： Wuyts A,Schutyser E,Menten P,Struyf S,D'Haese A,Bult H,Opdenakker G,Proost P,Van Damme J

更新日期：2000-11-28 00:00:00

abstract：:The deposition of amyloid-β (Aβ) plaques and tau-based neurofibrillary tangles is a neuropathological feature of Alzheimer's disease (AD). While studies have shown that the Aβ and tau interaction results in elevated AD pathology, the molecular linkage and mechanism of interaction of Aβ and tau are unclear. A recent st...

journal_title：Biochemistry

authors： Tripathi T,Khan H

更新日期：2020-02-04 00:00:00

abstract：:We previously showed that a bulky ring-strained cycloalkyne possessing a rhodamine fluorophore directly reacts (via strain-promoted click chemistry) with the azido group incorporated (via ligand-directed tosyl chemistry) into Asp160 in the 49 kDa subunit of complex I in bovine heart submitochondrial particles [Masuya,...

journal_title：Biochemistry

authors： Masuya T,Murai M,Ito T,Aburaya S,Aoki W,Miyoshi H

更新日期：2017-08-15 00:00:00

abstract：:Steady-state and femtosecond time-resolved optical methods have been used to compare the spectroscopic features and energy transfer dynamics of two systematically different light-harvesting complexes from the dinoflagellate Amphidinium carterae: main-form (MFPCP) and high-salt (HSPCP) peridinin-chlorophyll a-proteins....

journal_title：Biochemistry

authors： Ilagan RP,Koscielecki JF,Hiller RG,Sharples FP,Gibson GN,Birge RR,Frank HA

更新日期：2006-11-28 00:00:00

abstract：:The effect of concentrated glycerol on the thermal transitions of chymotrypsinogen and ribonuclease has been examined by differential spectrophotometry at 293 and 287 mm, respectively. It was found that for both proteins addition of glycerol raises the transition temperature, the increase in Tm being greater for ribon...

journal_title：Biochemistry

authors： Gekko K,Timasheff SN

更新日期：1981-08-04 00:00:00

abstract：:Purine repressor (PurR) binding to specific DNA is enhanced by complexing with purines, whereas lactose repressor (LacI) binding is diminished by interaction with inducer sugars despite 30% identity in their protein sequences and highly homologous tertiary structures. Nonetheless, in switching from low- to high-affini...

journal_title：Biochemistry

authors： Moraitis MI,Xu H,Matthews KS

更新日期：2001-07-10 00:00:00

abstract：:Doublet signal and singlet-like signal induced in Ca(2+)-depleted PS II were investigated by pulsed EPR in one-dimensionally oriented photosystem (PS) II membranes. The doublet signal showed marked angular dependent change in its spectrum in term of the applied magnetic field, indicating that the magnetic dipole-dipol...

journal_title：Biochemistry

authors： Mino H,Kawamori A,Ono T

更新日期：2000-09-12 00:00:00

abstract：:The Ca2+-ATPase of sarcoplasmic reticulum was purified and depleted of proteolipids by solubilization in Triton X-100 and by fractionation on a DE-52 column. The protein reconstituted by deoxycholate-cholate dialysis at low lipid to protein ratios (2-5 mg of lipid/mg of protein), with either dioleoylphosphatidylethano...

journal_title：Biochemistry

authors： Navarro J,Toivio-Kinnucan M,Racker E

更新日期：1984-01-03 00:00:00

abstract：:The oxygen transfer to p-hydroxybenzoate catalyzed by p-hydroxybenzoate hydroxylase (PHBH) has been shown to occur via a C4a-hydroperoxide of the flavin. Two factors are likely to be important in facilitating the transfer of oxygen from the C4a-hydroperoxide to the substrate. (a) The positive electrostatic potential o...

journal_title：Biochemistry

authors： Ortiz-Maldonado M,Aeschliman SM,Ballou DP,Massey V

更新日期：2001-07-31 00:00:00

abstract：:Campylobacter jejuni contains a general N-linked glycosylation pathway in which a heptasaccharide is sequentially assembled onto a polyisoprenyl diphosphate carrier and subsequently transferred to the asparagine side chain of an acceptor protein. The enzymes in the pathway function at a membrane interface and have in ...

journal_title：Biochemistry

authors： Chen MM,Weerapana E,Ciepichal E,Stupak J,Reid CW,Swiezewska E,Imperiali B

更新日期：2007-12-18 00:00:00

abstract：:The time course of changes in the intravesicular Ca2+ concentration ([Ca2+]i) in terminal cisternal sarcoplasmic reticulum vesicles upon the induction of Ca2+ release was investigated by using tetramethylmurexide (TMX) as an intravesicular Ca2+ probe. Upon the addition of polylysine at the concentration that led to th...

journal_title：Biochemistry

authors： Ikemoto N,Antoniu B,Kang JJ,Mészáros LG,Ronjat M

更新日期：1991-05-28 00:00:00

abstract：:Three methods, fluorescence anisotropy of rhodamine-labeled profilin, intrinsic fluorescence and nucleotide exchange, give the same affinity, Kd = 0.1 microM, for Acanthamoeba profilins binding amoeba actin monomers with bound Mg-ATP. Replacement of serine 38 with cysteine created a unique site where labeling with rho...

journal_title：Biochemistry

authors： Vinson VK,De La Cruz EM,Higgs HN,Pollard TD

更新日期：1998-08-04 00:00:00

abstract：:Recent studies on cytochrome oxidase have indicated that the putative "peroxy" intermediate in the catalytic cycle (P(R)) is a mixture of intermediates, including P and F [Sucheta, A., et al. (1998) Biochemistry 37, 17905-17914], and the bench-made P and F forms appear to have the same redox state (Fe(a3)(4+)=O(2-)), ...

journal_title：Biochemistry

authors： Van Eps N,Szundi I,Einarsdóttir O

更新日期：2003-05-06 00:00:00

abstract：:We are attempting to understand the processes required to accurately replicate the repetitive DNA sequences whose instability is associated with several human diseases. Here we test the hypothesis that the contribution of exonucleolytic proofreading to frameshift fidelity during replication of repetitive DNA sequences...

journal_title：Biochemistry

authors： Kroutil LC,Register K,Bebenek K,Kunkel TA

更新日期：1996-01-23 00:00:00

abstract：:The importance of the interdomain bridging interactions observed only in the R-state structure of Escherichia coli aspartate transcarbamylase between Glu-50 of the carbamoyl phosphate domain with both Arg-167 and Arg-234 of the aspartate domain has been investigated by using site-specific mutagenesis. Two mutant versi...

journal_title：Biochemistry

authors： Newton CJ,Kantrowitz ER

更新日期：1990-02-13 00:00:00

abstract：:The genome of all retroviruses consists of two identical RNAs noncovalently linked near their 5' end. Dimerization of genomic RNA is thought to modulate several steps in the retroviral life cycle, such as recombination, translation, and encapsidation. The kissing-loop model of HIV-1 genome dimerization [Laughrea, M., ...

journal_title：Biochemistry

authors： Laughrea M,Jetté L

更新日期：1996-02-06 00:00:00

abstract：:The concentration of hyaluronic acid, chondroitin sulfate, and heparan sulfate was measured in rat brain at 2-day intervals from birth to 1 month of age, and in 40-day-old and adult animals. The levels of all three glycosaminoglycans increased after birth to reach a peak at 7 days after which they declined steadily, a...

journal_title：Biochemistry

authors： Margolis RU,Margolis RK,Chang LB,Preti C

更新日期：1975-01-14 00:00:00

abstract：:PQQ is an exogenous, tricyclic, quino-cofactor for a number of bacterial dehydrogenases. The final step of PQQ formation is catalyzed by PqqC, a cofactorless oxidase. This study focuses on the activation of molecular oxygen in an enzyme active site without metal or cofactor and has identified a specific oxygen binding...

journal_title：Biochemistry

authors： RoseFigura JM,Puehringer S,Schwarzenbacher R,Toyama H,Klinman JP

更新日期：2011-03-08 00:00:00

abstract：:Vitronectin is a multifunctional plasma glycoprotein which may regulate the systems related to protease cascades such as the coagulation, fibrinolysis, and complement systems as well as cell adhesion. Solid-phase assays and affinity chromatography on immobilized glycolipids indicated that vitronectin purified under de...

journal_title：Biochemistry

authors： Yoneda A,Ogawa H,Kojima K,Matsumoto I

更新日期：1998-05-05 00:00:00

abstract：:Two novel heme-containing peroxidases, one able to incorporate halogens into aromatic substrates and the other able to remove them, have recently been isolated from marine sources and initially characterized by Chen et al. [(1991) J. Biol. Chem. 266, 23909-23915; (1996) J. Biol. Chem. 271, 4609-4612]. The haloperoxida...

journal_title：Biochemistry

authors： Roach MP,Chen YP,Woodin SA,Lincoln DE,Lovell CR,Dawson JH

更新日期：1997-02-25 00:00:00

abstract：:Synaptotagmin 1 (syt1) is a synaptic vesicle-anchored membrane protein that acts as the calcium sensor for the synchronous component of neuronal exocytosis. Using site-directed spin labeling, the position and membrane interactions of a fragment of syt1 containing its two C2 domains (syt1C2AB) were assessed in bilayers...

journal_title：Biochemistry

authors： Kuo W,Herrick DZ,Cafiso DS

更新日期：2011-04-05 00:00:00

abstract：:Decarboxylated S-adenosylmethionine was found to be a substrate for the nuclear acetyltransferases that act on polyamines and on histones. The rate of acetylation of decarboxylated S-adenosylmethionine was more than twice that of spermidine at saturating substrate concentrations, and decarboxylated S-adenosylmethionin...

journal_title：Biochemistry

authors： Pegg AE,Wechter RS,Clark RS,Wiest L,Erwin BG

更新日期：1986-01-28 00:00:00

abstract：:Microfibrils having a diameter of 10-12 nm, found either in association with elastin or independently, are an important component of the extracellular matrix of many tissues. Because isolation of native proteins composing the microfibrils has proven difficult, information on structure/function relationships is limited...

journal_title：Biochemistry

authors： Bashir MM,Abrams WR,Rosenbloom J,Kucich U,Bacarra M,Han MD,Brown-Augsberger P,Mecham R,Rosenbloom J

更新日期：1994-01-18 00:00:00

abstract：:The annexin fold consists of four 70-residue domains with markedly homologous sequences and nearly identical structures. Each domain contains five helices designated A to E. Domain 2 of annexin I was obtained by chemical synthesis including ten specifically labeled residues and studied by 1H-15N NMR and circular dichr...

journal_title：Biochemistry

authors： Cordier-Ochsenbein F,Guerois R,Baleux F,Huynh-Dinh T,Chaffotte A,Neumann JM,Sanson A

更新日期：1996-08-13 00:00:00

abstract：:A 19-amino acid residue peptide, Gly-Trp-Leu-Lys-Ile-Lys-Ala-Ala-Met-Arg-Trp-Gly-Phe-Phe-Val-Arg-Lys-Lys- Ala, corresponding to the basic amphiphilic alpha-helix (BAA) motif at the C-terminus of a recombinant tobacco calmodulin-binding protein, TCB60, was synthesized. The interaction of the synthetic binding domain wi...

journal_title：Biochemistry

authors： Dash S,Niemaczura W,Harrington HM

更新日期：1997-02-25 00:00:00

abstract：:Polycyclic aromatic hydrocarbon (PAH)-DNA adducts pervert the execution or fidelity of enzymatic DNA transactions and cause mutations and cancer. Here, we examine the effects of intercalating PAH-DNA adducts on the religation reaction of vaccinia DNA topoisomerase, a prototypal type IB topoisomerase (TopIB), and the 3...

journal_title：Biochemistry

authors： Yakovleva L,Handy CJ,Yagi H,Sayer JM,Jerina DM,Shuman S

更新日期：2006-06-20 00:00:00

abstract：:An alamethicin, secreted by the fungus Trichoderma viride and containing a glutamine at position 18 instead of the usual glutamic acid, has been uniformly labeled with 15N and purified by HPLC. The extent of 15N incorporation at individual backbone and side-chain sites was found to vary from 85% to 92%, as measured by...

journal_title：Biochemistry

authors： Yee AA,O'Neil JD

更新日期：1992-03-31 00:00:00

abstract：:The antimycotic drug clotrimazole inhibits the function of the gastric H,K-ATPase in a manner similar to that observed for the Na,K-ATPase. Because of the high hydrophobicity of the compound, the interaction between clotrimazole and the ion pump occurs at the membrane domain in the apolar core of the membrane. The enz...

journal_title：Biochemistry

authors： Witzke A,Lindner K,Munson K,Apell HJ

更新日期：2010-06-01 00:00:00

abstract：:Using our hemoglobin expression system in Escherichia coli, we have constructed three recombinant hemoglobins (rHbs) with amino acid substitutions located in the alpha(1)beta(1) and alpha(1)beta(2) subunit interfaces and in the distal heme pocket of the alpha-chain: rHb(alphaV96W, betaN108K), rHb(alphaL29F, alphaV96W,...

journal_title：Biochemistry

authors： Jeong ST,Ho NT,Hendrich MP,Ho C

更新日期：1999-10-05 00:00:00

abstract：:Complete 1H NMR sequential assignments have been made for copper(I)- and silver (I)-substituted metallothionein (MT) from Saccharomyces cerevisiae using standard 2D 1H NMR methods. The fingerprint region of the COSY spectrum of both metalloproteins shows a doubling of a few backbone proton resonances from residue K41 ...

journal_title：Biochemistry

authors： Narula SS,Winge DR,Armitage IM

更新日期：1993-07-06 00:00:00