Effect of lipid composition on the calcium/adenosine 5'-triphosphate coupling ratio of the Ca2+-ATPase of sarcoplasmic reticulum.

abstract：:Phosphatidic (PtdOH) acid formation is recognized as an important step in numerous signaling pathways in both plants and mammals. To study the role of this lipid in signaling pathways, it is of major interest to be able to increase the amount of this lipid directly. Therefore, "caged" PtdOH was synthesized, which rele...

journal_title：Biochemistry

authors： Goedhart J,Gadella TW Jr

更新日期：2004-04-13 00:00:00

abstract：:We have investigated, both experimentally and theoretically, the efflux of carboxyfluorescein (a self-quenching fluorescent dye) from vesicles of different sizes and lipid species (POPC, DOPC) after having added the bee venom peptide melittin. This comprises quantitative analyses regarding the extent of lipid-associat...

journal_title：Biochemistry

authors： Rex S,Schwarz G

更新日期：1998-02-24 00:00:00

abstract：:A synthetic analogue of the tripeptide hemiasterlin, designated HTI-286, depolymerizes microtubules, is a poor substrate for P-glycoprotein, and inhibits the growth of paclitaxel-resistant tumors in xenograft models. Two radiolabeled photoaffinity analogues of HTI-286, designated 4-benzoyl-N,beta,beta-trimethyl-l-phen...

journal_title：Biochemistry

authors： Nunes M,Kaplan J,Wooters J,Hari M,Minnick AA Jr,May MK,Shi C,Musto S,Beyer C,Krishnamurthy G,Qiu Y,Loganzo F,Ayral-Kaloustian S,Zask A,Greenberger LM

更新日期：2005-05-10 00:00:00

abstract：:The role of pyridoxal 5'-phosphate (PLP) in the radical-mediated amino group migration catalyzed by lysine 2,3-aminomutase from Clostridia SB4 has been investigated by electron spin echo envelope modulation (ESEEM) spectroscopy. This pulsed electron paramagnetic resonance (EPR) method was used to estimate the distance...

journal_title：Biochemistry

authors： Ballinger MD,Frey PA,Reed GH,LoBrutto R

更新日期：1995-08-08 00:00:00

abstract：:Tyramine β-monooxygenase (TβM), the insect homologue of dopamine β-monooxygenase, is a neuroregulatory enzyme that catalyzes the β-hydroxylation of tyramine to yield octopamine. Mutation of the methionine (Met) ligand to Cu(M) of TβM, Met471Cys, yielded a form of TβM that is catalytically active but susceptible to ina...

journal_title：Biochemistry

authors： Osborne RL,Zhu H,Iavarone AT,Hess CR,Klinman JP

更新日期：2012-09-25 00:00:00

abstract：:Temporins are short (10-13 amino acids) and linear antimicrobial peptides first isolated from the skin of the European red frog, Rana temporaria, and are effective against Gram-positive bacteria and Candida albicans. To get insight into their mechanism(s) of action, we compared the effects on model membranes exerted b...

journal_title：Biochemistry

authors： Zhao H,Rinaldi AC,Di Giulio A,Simmaco M,Kinnunen PK

更新日期：2002-04-02 00:00:00

abstract：:Horseradish peroxidase (HRP) was oxidized by IrCl6(2-) to a mixture of compounds I and II, the rate of oxidation and the ratio of the mixture being greatly affected by pH (Hayashi & Yamazaki, 1979). Oxidation of HRP by IrCl6(2-) in the presence of fluoride was significantly accelerated. This resulted in the formation ...

journal_title：Biochemistry

authors： Farhangrazi ZS,Sinclair R,Powers L,Yamazaki I

更新日期：1995-11-21 00:00:00

abstract：:Zinc finger nucleases (ZFNs) have been used to direct precise modifications of the genetic information in living cells at high efficiency. An important consideration in the design of ZFNs is the number of zinc fingers that are required for efficient and specific cleavage. We examined dimeric ZFNs composed of [1]+[1], ...

journal_title：Biochemistry

authors： Shimizu Y,Şöllü C,Meckler JF,Adriaenssens A,Zykovich A,Cathomen T,Segal DJ

更新日期：2011-06-07 00:00:00

abstract：:Despite intense interest in the proteolysis of the β-Amyloid Precursor Protein (APP) in Alzheimer's disease, how the normal processing of this type I receptor-like glycoprotein is physiologically regulated remains ill-defined. In recent years, several candidate protein ligands for APP, including F-spondin, Reelin, β1 ...

journal_title：Biochemistry

authors： Rice HC,Young-Pearse TL,Selkoe DJ

更新日期：2013-05-14 00:00:00

abstract：:Electron nuclear double resonance (ENDOR) of protons at Type 2 and Type 1 cupric active sites correlates with the enzymatic pH dependence, the mutation of nearby conserved, nonligating residues, and electron transfer in heterologously expressed Rhodobacter sphaeroides nitrite reductase. Wild-type enzyme showed a pH 6 ...

journal_title：Biochemistry

authors： Zhao Y,Lukoyanov DA,Toropov YV,Wu K,Shapleigh JP,Scholes CP

更新日期：2002-06-11 00:00:00

abstract：:Recent studies of proteins with reversed charged residues have demonstrated that electrostatic interactions on the surface can contribute significantly to protein stability. We have used the approach of reversing negatively charged residues using Arg to evaluate the effect of the electrostatics context on the transiti...

journal_title：Biochemistry

authors： Mancusso R,Cruz E,Cataldi M,Mendoza C,Fuchs J,Wang H,Yang X,Tasayco ML

更新日期：2004-04-06 00:00:00

abstract：:3-(Trifluoromethyl)-3-(m-iodophenyl)diazirine (TID) has recently been found to be a noncompetitive inhibitor of the nicotinic acetylcholine receptor (nAcChoR) by both photolabeling and flux assays (White et al., 1991). However, these experiments were done when TID was in equilibrium with the nAcChoR, and thus only its...

journal_title：Biochemistry

authors： Wu G,Raines DE,Miller KW

更新日期：1994-12-27 00:00:00

abstract：:The bacterial reaction center (RC) converts light into chemical energy through the reduction of an internal quinone molecule Q(B) to Q(B)H(2). In the native RC, proton transfer is coupled to electron transfer and is not rate-controlling. Consequently, proton transfer is not directly observable, and its rate was unknow...

journal_title：Biochemistry

authors： Paddock ML,Adelroth P,Feher G,Okamura MY,Beatty JT

更新日期：2002-12-17 00:00:00

abstract：:The neonatal Fc receptor (FcRn) binds maternal immunoglobulin G (IgG) during the acquisition of passive immunity by the fetus or newborn. In adult mammals, FcRn also binds IgG and returns it to the bloodstream, thus protecting IgG from a default degradative pathway. Biosensor assays have been used to characterize the ...

journal_title：Biochemistry

authors： Vaughn DE,Bjorkman PJ

更新日期：1997-08-05 00:00:00

abstract：:N-(1-Pyrenyl)iodoacetamide has been used to introduce fluorescent probes into Escherichia coli RNA polymerase. After an incubation time of 15 min, approximately 2 pyrene equiv was introduced per enzyme molecule. There was no further increase in modification after more extended periods of incubation. Neither calf thymu...

journal_title：Biochemistry

authors： Johnson RS,Bowers M,Eaton Q

更新日期：1991-01-08 00:00:00

abstract：:Terminase is an enzyme from bacteriophage lambda that is required for insertion of the viral genome into an empty pro-capsid. This enzyme is composed of the viral proteins gpNu1 (20.4 kDa) and gpA (73.3 kDa) in a holoenzyme complex. Current models for terminase assembly onto DNA suggest that gpNu1 binds to three repea...

journal_title：Biochemistry

authors： Yang Q,de Beer T,Woods L,Meyer JD,Manning MC,Overduin M,Catalano CE

更新日期：1999-01-05 00:00:00

abstract：:The insulin hexamer is an allosteric protein which displays positive and negative cooperativity and half-site reactivity that is modulated by strong homotropic and heterotropic ligand binding interactions at two different loci. These loci consist of phenolic pockets situated on the dimer-dimer interfaces of T-R and R-...

journal_title：Biochemistry

authors： Huang ST,Choi WE,Bloom C,Leuenberger M,Dunn MF

更新日期：1997-08-12 00:00:00

abstract：:Glycosylation is a common modification that can endow proteins with altered physical and biological properties. Ribonuclease 1 (RNase 1), which is the human homologue of the archetypal enzyme RNase A, undergoes N-linked glycosylation at asparagine residues 34, 76, and 88. We have produced the three individual glycofor...

journal_title：Biochemistry

authors： Kilgore HR,Latham AP,Ressler VT,Zhang B,Raines RT

更新日期：2020-09-01 00:00:00

abstract：:Cyanobacteriochromes (CBCRs) are cyanobacterial photoreceptors distantly related to phytochromes. Like phytochromes, CBCRs photointerconvert between two photostates that accompany photoisomerization of their bilin chromophores. While phytochromes typically exhibit red/far-red photocycles, CBCR photocycles are much mor...

journal_title：Biochemistry

authors： Gottlieb SM,Kim PW,Corley SC,Madsen D,Hanke SJ,Chang CW,Rockwell NC,Martin SS,Lagarias JC,Larsen DS

更新日期：2014-02-18 00:00:00

abstract：:A general kinetic model for catalysis by interfacial enzymes is developed. It couples the Michaelis-Menten catalytic turnover cycle at the interface with that in the aqueous phase through the distribution equilibria between the interface and the surrounding aqueous phase. Analysis under two limiting conditions fully d...

journal_title：Biochemistry

authors： Berg OG,Rogers J,Yu BZ,Yao J,Romsted LS,Jain MK

更新日期：1997-11-25 00:00:00

abstract：:Paramagnetic agents produce line broadening and thus cancellation of anti phase cross-peak components in two-dimensional correlated nuclear magnetic resonance spectra. The specificity of this effect was examined to determine its utility for identifying surface residues of proteins. Ubiquitin and hen egg white lysozyme...

journal_title：Biochemistry

authors： Petros AM,Mueller L,Kopple KD

更新日期：1990-10-30 00:00:00

abstract：:SH2 domain-containing inositol 5-phosphatases 1 (SHIP1) and 2 (SHIP2) are structurally similar proteins that catalyze the degradation of lipid secondary messenger phosphatidylinositol 3,4,5-triphosphate to produce phosphatidylinositol 3,4-diphosphate. Despite their high sequence identity (51%), SHIP1 and SHIP2 share l...

journal_title：Biochemistry

authors： Zhang Y,Wavreille AS,Kunys AR,Pei D

更新日期：2009-11-24 00:00:00

abstract：:The disulfide structure of sillucin, a highly knotted, cysteine-rich, antimicrobial peptide, isolated from Rhizomucor pusillus, has been determined to be Cys2--Cys7, Cys12--Cys24, Cys13--Cys30, and Cys14--Cys21 by disulfide mass mapping based on partial reduction and CN-induced cleavage enabled by cyanylation. The den...

journal_title：Biochemistry

authors： Qi J,Wu J,Somkuti GA,Watson JT

更新日期：2001-04-17 00:00:00

abstract：:cis-3-Chloroacrylic acid dehalogenase (cis-CaaD) from Pseudomonas pavonaceae 170 and a homologue from Corynebacterium glutamicum designated Cg10062 are 34% identical in sequence (54% similar). The former catalyzes a key step in a bacterial catabolic pathway for the nematocide 1,3-dichloropropene, whereas the latter ha...

journal_title：Biochemistry

authors： Schroeder GK,Huddleston JP,Johnson WH Jr,Whitman CP

更新日期：2013-06-18 00:00:00

abstract：:In the processes of DNA replication, recombination, and repair, duplex DNA can transiently form branched structures, such as Holliday junctions, as well as base pair mismatches and bulges. These stages have altered ligand and protein binding properties from normal double helical DNA. A variety of ligands have been rep...

journal_title：Biochemistry

authors： Hernández LI,Zhong M,Courtney SH,Marky LA,Kallenbach NR

更新日期：1994-11-08 00:00:00

abstract：:Quorum sensing, the ability of bacteria to sense their own population density through the synthesis and detection of small molecule signals, has received a great deal of attention in recent years. Acyl homoserine lactones (AHLs) are a major class of quorum sensing signaling molecules. In nature, some bacteria that do ...

journal_title：Biochemistry

authors： Chowdhary PK,Keshavan N,Nguyen HQ,Peterson JA,González JE,Haines DC

更新日期：2007-12-18 00:00:00

abstract：:8-OxoGua (8-oxo-7,8-dihydroguanine) is produced in nucleic acids as well as in nucleotide pools of cells, by reactive oxygen species normally formed during cellular metabolic processes. MutT protein of Escherichia coli specifically degrades 8-oxoGua-containing deoxyribo- and ribonucleoside triphosphates to correspondi...

journal_title：Biochemistry

authors： Ito R,Hayakawa H,Sekiguchi M,Ishibashi T

更新日期：2005-05-03 00:00:00

abstract：:The proline catabolic enzyme Δ(1)-pyrroline-5-carboxylate dehydrogenase (ALDH4A1) catalyzes the NAD(+)-dependent oxidation of γ-glutamate semialdehyde to l-glutamate. In Saccharomyces cerevisiae, ALDH4A1 is encoded by the PUT2 gene and known as Put2p. Here we report the steady-state kinetic parameters of the purified ...

journal_title：Biochemistry

authors： Pemberton TA,Srivastava D,Sanyal N,Henzl MT,Becker DF,Tanner JJ

更新日期：2014-03-04 00:00:00

abstract：:The gene encoding enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system from an Escherichia coli enzyme I mutant was cloned and sequenced. The mutation was shown to be a guanine to adenine transition resulting in an altered protein in which glycine-338 was replaced by aspartic acid. The enzyme I structur...

journal_title：Biochemistry

authors： Seok YJ,Lee BR,Gazdar C,Svenson I,Yadla N,Peterkofsky A

更新日期：1996-01-09 00:00:00

abstract：:It has been shown (Poole et al., 1991) that deletion of residues 44-49 from the sequence of staphylococcal nuclease (E43 SNase) results in an enzyme (E43 delta SNase) that is significantly more active than D43 SNase, an enzyme that differs from the wild-type enzyme by deletion of a single methylene group. In addition,...

journal_title：Biochemistry

authors： Baldisseri DM,Torchia DA,Poole LB,Gerlt JA

更新日期：1991-04-16 00:00:00