Photolysis of caged phosphatidic acid induces flagellar excision in Chlamydomonas.

abstract：:Ultraviolet thermal denaturation studies substantiate our earlier hypothesis that substitution of a L-nucleotide residue for a D-nucleotide within a DNA duplex permits a stable structure in which all bases are paired through Watson-Crick hydrogen bonds (Damha, M. J., Giannaris, P. A., Marfey, P., & Reid, L. S. (1991) ...

journal_title：Biochemistry

authors： Damha MJ,Giannaris PA,Marfey P

更新日期：1994-06-28 00:00:00

abstract：:Anticoagulation factor II (ACF II) isolated from the venom of Agkistrodon acutus is an activated coagulation factor X-binding protein in a Ca(2+)-dependent fashion with marked anticoagulant activity. The equilibrium unfolding/refolding of apo-ACF II, holo-ACF II, and Tb(3+)-reconstituted ACF II in guanidine hydrochlor...

journal_title：Biochemistry

authors： Xu X,Liu Q,Xie Y

更新日期：2002-03-19 00:00:00

abstract：:The thermotropic behavior of 20 chemically related glycosphingolipids (GSLs) of high purity, containing neutral and anionic carbohydrate residues in their oligosaccharide chains, was studied by high-sensitivity differential scanning calorimetry. In general, the polar head group of GSLs appears to be one of the major d...

journal_title：Biochemistry

authors： Maggio B,Ariga T,Sturtevant JM,Yu RK

更新日期：1985-02-26 00:00:00

abstract：:S100A1, a member of the S100 protein family, is an EF-hand containing Ca(2+)-binding protein (93 residues per subunit) with noncovalent interactions at its dimer interface. Each subunit of S100A1 has four alpha-helices and a small antiparallel beta-sheet consistent with two helix-loop-helix calcium-binding domains [Ba...

journal_title：Biochemistry

authors： Rustandi RR,Baldisseri DM,Inman KG,Nizner P,Hamilton SM,Landar A,Landar A,Zimmer DB,Weber DJ

更新日期：2002-01-22 00:00:00

abstract：:The structure of cytochrome f includes an internal chain of five water molecules and six hydrogen-bonding side chains, which are conserved throughout the phylogenetic range of photosynthetic organisms from higher plants, algae, and cyanobacteria. The in vivo electron transfer capability of Chlamydomonas reinhardtii cy...

journal_title：Biochemistry

authors： Sainz G,Carrell CJ,Ponamarev MV,Soriano GM,Cramer WA,Smith JL

更新日期：2000-08-08 00:00:00

abstract：:DNA polymerases slide on DNA during replication, and the interface must be mobile for various conformational changes. The role of lubricant interfacial water is not understood. In this report, we systematically characterize the water dynamics at the interface and in the active site of a tight binding polymerase (pol β...

journal_title：Biochemistry

authors： Yang Y,Qin Y,Ding Q,Bakhtina M,Wang L,Tsai MD,Zhong D

更新日期：2014-08-26 00:00:00

abstract：:X-ray crystal structures of enzyme-ligand complexes are widely believed to mimic states in the catalytic cycle, but this presumption has seldom been carefully scrutinized. In the case of Tritrichomonas foetus inosine 5'-monophosphate dehydrogenase (IMPDH), 10 structures of various enzyme-substrate-inhibitor complexes ...

journal_title：Biochemistry

authors： Josephine HR,Ravichandran KR,Hedstrom L

更新日期：2010-12-21 00:00:00

abstract：:A specific requirement for lipids, particularly cardiolipin (CL), in cytochrome c oxidase (CcO) has been reported in many previous studies using mainly in vitro lipid removal approaches in mammalian systems. Our accompanying paper shows that CcO produced in markedly CL-depleted Rhodobacter sphaeroides displays wild-ty...

journal_title：Biochemistry

authors： Zhang X,Hiser C,Tamot B,Benning C,Reid GE,Ferguson-Miller SM

更新日期：2011-05-17 00:00:00

abstract：:The lipid A disaccharide of the Escherichia coli envelope is synthesized from the two fatty acylated glucosamine derivatives UDP-N2,O3-bis[(R)-3-hydroxytetradecanoyl]-alpha-D- glucosamine (UDP-2,3-diacyl-GlcN) and N2,O3-bis[(R)-3-hydroxytetradecanoyl]-alpha-D-glucosamine 1-phosphate (2,3-diacyl-GlcN-1-P) [Ray, B. L., ...

journal_title：Biochemistry

authors： Anderson MS,Robertson AD,Macher I,Raetz CR

更新日期：1988-03-22 00:00:00

abstract：:The interaction of poly[(G-C)] and poly[d(G-m5C)] with the antitumor antibiotic elsamicin A, which binds to alternating guanine + cytosine tracts in DNA, has been studied under the B and Z conformations. Both the rate and the extent of the B-to-Z transition are diminished by the antibiotic, as inferred by spectroscopi...

journal_title：Biochemistry

authors： Jiménez-García E,Portugal J

更新日期：1992-11-24 00:00:00

abstract：:Amyloid beta peptide (A beta) is released into the media of a variety of cells in culture during normal metabolism. The discovery of several missense mutations within or flanking the A beta region of the beta amyloid precursor protein (beta APP) in familial Alzheimer's disease provides strong evidence for a role of al...

journal_title：Biochemistry

authors： Querfurth HW,Selkoe DJ

更新日期：1994-04-19 00:00:00

abstract：:The channel-forming protein OmpF porin from Escherichia coli spans the bacterial outer membrane. Each of the three monomers comprises a hollow, 16-stranded beta-barrel. These are associated to homotrimers which are unusually stable, due mostly to hydrophobic interactions between the beta-barrels. In addition, a loop, ...

journal_title：Biochemistry

authors： Phale PS,Philippsen A,Kiefhaber T,Koebnik R,Phale VP,Schirmer T,Rosenbusch JP

更新日期：1998-11-10 00:00:00

abstract：:Resonance energy transfer measurements provide a way to estimate distances between chromophores attached to different sites of macromolecules. There are two unknowns involved in resonance energy transfer measurements, the distance between two chromophores and their relative orientation. When static orientational disor...

journal_title：Biochemistry

authors： Wu P,Brand L

更新日期：1992-09-01 00:00:00

abstract：:To identify the elements which regulate the liver transcription of the human type II phospholipase A2 gene and its stimulation by interleukin 6, the 5' flanking region from -1614 to +806 and several 3' and 5' deleted fragments have been analyzed in CAT assays. Negative regulatory elements have been located in the regi...

journal_title：Biochemistry

authors： Olivier JL,Fan Q,Salvat C,Ziari M,Kong L,Mangeney M,Bereziat G

更新日期：1994-06-14 00:00:00

abstract：:Overall activation volumes for multistep reactions are not usually pressure independent. The present investigation gives a quantitative description of this effect under Theory. Simple relations are obtained which can easily be applied to experimental data and which allow more insight into the dynamics of enzyme reacti...

journal_title：Biochemistry

authors： Butz P,Greulich KO,Ludwig H

更新日期：1988-03-08 00:00:00

abstract：:In a family of proteins, often the three-dimensional structure has been experimentally determined only for one member or a few members of the family. Homology modeling can be used to model the structures of all other members of the family and thus allow comparison of these structures. This approach was applied to hete...

journal_title：Biochemistry

authors： Kosloff M,Elia N,Selinger Z

更新日期：2002-12-10 00:00:00

abstract：:The noncovalent interaction of 2-aminodipyrido[1,2-a:3',2'-d]imidazole (Glu-P-2) and its derivatives, which are potent mutagens isolated from L-glutamic acid pyrolysate, with calf thymus DNA was studied by steady-state and nanosecond fluorescence spectroscopies. The fluorescence of these compounds exhibits static quen...

journal_title：Biochemistry

authors： Itoh M,Kurokawa H,Usui M,Ohno M,Shimoda E,Hashimoto Y,Shudo K

更新日期：1985-11-05 00:00:00

abstract：:Infrared spectroscopy has been used to monitor residual ordered structure in the denatured state of wild-type and two mutants of iso-1-cytochrome c. The technique used involves a careful digital subtraction procedure that removes spectral contributions from buffer, water vapor, and the denaturant guanidine hydrochlori...

journal_title：Biochemistry

authors： Bowler BE,Dong A,Caughey WS

更新日期：1994-03-08 00:00:00

abstract：:The preferential interactions of proteins with solvent components were studied in concentrated aqueous solutions of the sulfate, acetate, and chloride salts of Mg2+, Ba2+, Ca2+, Mn2+ and Ni2+ [except for CaSO4, BaSO4, Mn-(OAc)2, and Ni(OAc)2], and results were compared with those of the Na+ salts. It was found that, f...

journal_title：Biochemistry

authors： Arakawa T,Timasheff SN

更新日期：1984-12-04 00:00:00

abstract：:The deposition of fibrils and amorphous aggregates of transthyretin (TTR) in patient tissues is a hallmark of TTR amyloid disease, but the molecular details of amyloidogenesis are poorly understood. Tetramer dissociation is typically rate-limiting for TTR amyloid fibril formation, so we have used a monomeric variant o...

journal_title：Biochemistry

authors： Hurshman AR,White JT,Powers ET,Kelly JW

更新日期：2004-06-15 00:00:00

abstract：:A new specific voltage-sensitive calcium channel (VSCC) blocker has been isolated from the venom of the fish-hunting cone snail Conus consors. This peptide, named omega-Ctx CNVIIA, consists of 27 amino acid residues folded by 3 disulfide bridges. Interestingly, loop 4, which is supposed to be crucial for selectivity, ...

journal_title：Biochemistry

authors： Favreau P,Gilles N,Lamthanh H,Bournaud R,Shimahara T,Bouet F,Laboute P,Letourneux Y,Ménez A,Molgó J,Le Gall F

更新日期：2001-12-04 00:00:00

abstract：:Toxic shock syndrome toxin-1 (TSST-1), the potent staphylococcal exoprotein linked to most cases of the toxic shock syndrome, is a V beta-restricted T-cell mitogen (a so-called "superantigen"). TSST-ovine (TSST-O) is a natural variant of TSST-1, and is produced by certain ovine mastitis-associated strains of Staphyloc...

journal_title：Biochemistry

authors： Deresiewicz RL,Woo J,Chan M,Finberg RW,Kasper DL

更新日期：1994-11-01 00:00:00

abstract：:The structure of isocitrate dehydrogenase (IDH) with a bound complex of isocitrate, NADP+, and Ca2+ was solved at 2.5-A resolution and compared by difference mapping against previously determined enzymatic complexes. Calcium replaces magnesium in the binding of metal-substrate chelate complex, resulting in a substanti...

journal_title：Biochemistry

authors： Stoddard BL,Dean A,Koshland DE Jr

更新日期：1993-09-14 00:00:00

abstract：:Tec family nonreceptor tyrosine kinases are key immunological enzymes that control processes that range from T and B cell development to reorganization of the actin cytoskeleton. The full-length Tec kinases have been resistant to crystallization. This lack of structural data and the paucity of in vitro biochemical dat...

journal_title：Biochemistry

authors： Joseph RE,Min L,Andreotti AH

更新日期：2007-05-08 00:00:00

abstract：:Cadherins are single pass transmembrane glycoproteins which mediate calcium dependent cell-cell adhesion by homophilic interactions. To reveal the molecular details of calcium binding and homoassociation, we recombinantly expressed in Escherichia coli a domain pair consisting of the first two domains of E-cadherin (EC...

journal_title：Biochemistry

authors： Koch AW,Pokutta S,Lustig A,Engel J

更新日期：1997-06-24 00:00:00

abstract：:Site-specifically modified oligodeoxynucleotides were used to explore the influence of neighboring base sequence context on the mutagenic potential of N-(deoxyguanosin-8-yl)-2-acetylaminofluorene (dG-AAF) and N-(deoxyguanosin-8-yl)-2-aminofluorene (dG-AF) in mammalian cells. Oligodeoxynucleotides ((5)(')TCCTCCTNXNCTCT...

journal_title：Biochemistry

authors： Shibutani S,Suzuki N,Tan X,Johnson F,Grollman AP

更新日期：2001-03-27 00:00:00

abstract：:The understudied nonribosomal-peptide-synthetase-independent siderophore (NIS) synthetase family has been increasingly associated with virulence in bacterial species due to its key role in the synthesis of hydroxamate and carboxylate "stealth" siderophores. We have identified a model family member, DesD, from Streptom...

journal_title：Biochemistry

authors： Hoffmann KM,Goncuian ES,Karimi KL,Amendola CR,Mojab Y,Wood KM,Prussia GA,Nix J,Yamamoto M,Lathan K,Orion IW

更新日期：2020-09-22 00:00:00

abstract：:Designing subtype-selective agonists for neuronal nicotinic acetylcholine receptors is a challenging and significant goal aided by intricate knowledge of each subtype's binding patterns. We previously reported that in α6β2 receptors, acetylcholine makes a functional cation-π interaction with Trp149, but nicotine and T...

journal_title：Biochemistry

authors： Post MR,Lester HA,Dougherty DA

更新日期：2017-04-04 00:00:00

abstract：:Aspartate transcarbamoylase from Escherichia coli is a dodecameric enzyme consisting of two trimeric catalytic subunits and three dimeric regulatory subunits. The X-ray structure of this enzyme indicates that the side chains of His-41, Asp-100, and Asp-90 from one catalytic chain form interactions with the side chains...

journal_title：Biochemistry

authors： Baker DP,Kantrowitz ER

更新日期：1993-09-28 00:00:00

abstract：:The light-induced QA-/QA FTIR difference spectrum of the photoreduction of the primary quinone (QA) in reaction centers (RCs) from Rhodobacter sphaeroides exhibits a set of complex differential bands between 1750 and 1715 cm(-1). Several of these features correspond in frequency to bands that bleach in the HA-/HA FTIR...

journal_title：Biochemistry

authors： Breton J,Nabedryk E,Allen JP,Williams JC

更新日期：1997-04-15 00:00:00