当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Pulsed electron paramagnetic resonance studies of the lysine 2,3-aminomutase substrate radical: evidence for participation of pyridoxal 5'-phosphate in a radical rearrangement.

Pulsed electron paramagnetic resonance studies of the lysine 2,3-aminomutase substrate radical: evidence for participation of pyridoxal 5'-phosphate in a radical rearrangement.

Abstract：

:The role of pyridoxal 5'-phosphate (PLP) in the radical-mediated amino group migration catalyzed by lysine 2,3-aminomutase from Clostridia SB4 has been investigated by electron spin echo envelope modulation (ESEEM) spectroscopy. This pulsed electron paramagnetic resonance (EPR) method was used to estimate the distance between the unpaired electron in the alpha-radical of beta-lysine, a steady-state intermediate in the reaction, and deuterium at the C4' position of the cofactor, PLP. [4'-2H]PLP was synthesized and exchanged into the enzyme. The steady-state radical was generated in the labeled samples and in samples with unlabeled PLP by addition of L-lysine.H2SO4 to activated enzyme. ESEEM spectra of the samples prepared with [4'-2H]PLP exhibited distinctive low-frequency modulations that were not present in spectra of matched samples with unlabeled PLP. Fourier transformation of the modulations yielded a prominent doublet signal centered about the Larmor frequency of deuterium. The magnitude of the doublet splitting of the 2H ESEEM signal exhibited angle selection across the CW EPR powder pattern. The observed angle selection, as well as simulation of the time domain spectra, indicated that the doublet splitting was due to the combined effects of the 2H hyperfine and nuclear quadrupole interactions. The influences of the quadrupole interaction and of isotropic and dipolar hyperfine interactions were explored by simulations of the ESEEM spectra. The analysis indicates a distance of < 3.5 A between the 2H at C4' of PLP and the radical center at C alpha lysine. The data are most compatible with an aldimine linkage between PLP and the beta-nitrogen of beta-lysine.(ABSTRACT TRUNCATED AT 250 WORDS)

journal_name

Biochemistry

journal_title

Biochemistry

authors

Ballinger MD,Frey PA,Reed GH,LoBrutto R

doi

10.1021/bi00031a033

subject

Has Abstract

pub_date

1995-08-08 00:00:00

pages

10086-93

issue

31

eissn

0006-2960

issn

1520-4995

journal_volume

34

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Identification of catalytic residues in the As(III) S-adenosylmethionine methyltransferase.

    abstract：:The enzyme As(III) S-adenosylmethionine methyltransferase (EC 2.1.1.137) (ArsM or AS3MT) is found in members of every kingdom, from bacteria to humans. In these enzymes, there are three conserved cysteine residues at positions 72, 174, and 224 in the CmArsM orthologue from the thermophilic eukaryotic alga Cyanidioschy...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi201500c

    authors： Marapakala K,Qin J,Rosen BP

    更新日期：2012-02-07 00:00:00

  • We FRET so You Don't Have To: New Models of the Lipoprotein Lipase Dimer.

    abstract：:Lipoprotein lipase (LPL) is a dimeric enzyme that is responsible for clearing triglyceride-rich lipoproteins from the blood. Although LPL plays a key role in cardiovascular health, an experimentally derived three-dimensional structure has not been determined. Such a structure would aid in understanding mutations in LP...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.7b01009

    authors： Hayne CK,Yumerefendi H,Cao L,Gauer JW,Lafferty MJ,Kuhlman B,Erie DA,Neher SB

    更新日期：2018-01-16 00:00:00

  • Neurotoxins active on insects: amino acid sequences, chemical modifications, and secondary structure estimation by circular dichroism of toxins from the scorpion Androctonus australis Hector.

    abstract：:Two scorpion neurotoxins active only on insects, the insect toxins AaH IT1 and AaH IT2, were purified from the venom of scorpions Androctonus australis Hector collected in Tozeur (Tunisia) and characterized. AaH IT2 was sequenced and found to differ in four amino acid positions from AaH IT, the single previously seque...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00458a021

    authors： Loret EP,Mansuelle P,Rochat H,Granier C

    更新日期：1990-02-13 00:00:00

  • The axial tyrosinate Fe3+ ligand in protocatechuate 3,4-dioxygenase influences substrate binding and product release: evidence for new reaction cycle intermediates.

    abstract：:The essential active site Fe3+ of protocatechuate 3,4-dioxygenase [3, 4-PCD, subunit structure (alphabetaFe3+)12] is bound by axial ligands, Tyr447 (147beta) and His462 (162beta), and equatorial ligands, Tyr408 (108beta), His460 (160beta), and a solvent OH- (Wat827). Recent X-ray crystallographic studies have shown th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi972047b

    authors： Frazee RW,Orville AM,Dolbeare KB,Yu H,Ohlendorf DH,Lipscomb JD

    更新日期：1998-02-24 00:00:00

  • Role of metal ions in catalysis by enolase: an ordered kinetic mechanism for a single substrate enzyme.

    abstract：:Spectroscopic and kinetic methods have been used to explore the roles of divalent metal ions in the enolase-catalyzed dehydration of 2-phosphoglycerate (2-PGA). Enolase requires 2 equiv of metal ion per active site for maximal activity. Previous crystallographic studies [Larsen, T. M., Wedekind, J. E., Rayment, I., an...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0103922

    authors： Poyner RR,Cleland WW,Reed GH

    更新日期：2001-07-10 00:00:00

  • Infrared spectroscopic study of a "hard-to-exchange" phospholipid population in the vicinity of CaATPase.

    abstract：:CaATPase from rabbit sarcoplasmic reticulum (SR) has been isolated, purified, and reconstituted into unilamellar vesicles with 1,2-dipentadecanoylphosphatidylcholine (DPePC) and acyl-chain-perdeuterated 1,2-dipalmitoylphosphatidylcholine (DPPC-d62). For total lipid:protein mole ratios between 25 and 70, a constant "ha...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00075a024

    authors： Senak L,Mendelsohn R

    更新日期：1993-06-22 00:00:00

  • Light-induced conformational changes in photosynthetic reaction centers: impact of detergents and lipids on the electronic structure of the primary electron donor.

    abstract：:Light-induced hypsochromic shifts of the Q(y) absorption band of the bacteriochlorophyll dimer (P) from 865 to 850 nm were identified using continuous illumination of dark-adapted reaction centers (RCs) from Rhodobacter capsulatus when dispersed in the most commonly used detergent, the zwitterionic lauryl N-dimethylam...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi200595z

    authors： Deshmukh SS,Akhavein H,Williams JC,Allen JP,Kalman L

    更新日期：2011-06-14 00:00:00

  • Spin equilibrium in human methemoglobin: effects of inositol hexaphosphate and bezafibrate as measured by resonance Raman spectroscopy.

    abstract：:The spin equilibria of several derivatives of human methemoglobin were probed by resonance Raman scattering. The intensity of lines in the Raman spectrum gives a measure of the high-spin (S = 5/2) to low-spin (S = 1/2) ratio which agrees well with the spin equilibria determined from direct magnetic susceptibility meas...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00438a056

    authors： Noble RW,DeYoung A,Rousseau DL

    更新日期：1989-06-13 00:00:00

  • Altering the Enantioselectivity of Tyrosyl-tRNA Synthetase by Insertion of a Stereospecific Editing Domain.

    abstract：:Translation of mRNAs by the ribosome is stereospecific, with only l-amino acids being incorporated into the nascent polypeptide chain. This stereospecificity results from the exclusion of d-amino acids at three steps during protein synthesis: (1) the aminoacylation of tRNA by aminoacyl-tRNA synthetases, (2) binding of...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b01167

    authors： Richardson CJ,First EA

    更新日期：2016-03-15 00:00:00

  • A New Covalent Inhibitor of Class C β-Lactamases Reveals Extended Active Site Specificity.

    abstract：:O-Aryloxycarbonyl hydroxamates have previously been shown to efficiently inactivate class C β-lactamases by cross-linking serine and lysine residues in the active site. A new analogue of these inhibitors, D-(R)-O-(phenoxycarbonyl)-N-[(4-amino-4-carboxy-1-butyl)oxycarbonyl]hydroxylamine, designed to inactivate certain ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b01149

    authors： Tilvawala R,Cammarata M,Adediran SA,Brodbelt JS,Pratt RF

    更新日期：2015-12-22 00:00:00

  • An additional copy of the homologous region (hr1) sequence in the Autographa californica multinucleocapsid polyhedrosis virus genome promotes hyperexpression of foreign genes.

    abstract：:The Autographa californica multinucleocapsid nuclear polyhedrosis virus genome contains nine homologous region (hr1, hr1a, hr2, hr2a, hr3, hr4a, hr4b, hr4c, and hr5) sequences that are thought to be involved in viral replication and activation of transcription. Our results show that the 750 bp hr1 sequence is capable ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi049953q

    authors： Venkaiah B,Viswanathan P,Habib S,Hasnain SE

    更新日期：2004-06-29 00:00:00

  • Binding of a photoaffinity analogue of glutathione to MRP1 (ABCC1) within two cytoplasmic regions (L0 and L1) as well as transmembrane domains 10-11 and 16-17.

    abstract：:MRP1 (or ABCC1) is an ABC membrane protein that transports a wide range of natural products as well as glutathione (GSH)-, glucuronate-, and sulfate-conjugated metabolites. In addition, free GSH is required for MRP1 to transport several chemotherapeutic drugs. However, the mechanisms regulating the influence of GSH on...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0268807

    authors： Karwatsky J,Daoud R,Cai J,Gros P,Georges E

    更新日期：2003-03-25 00:00:00

  • Identification and characterization of PshB, the dicluster ferredoxin that harbors the terminal electron acceptors F(A) and F(B) in Heliobacterium modesticaldum.

    abstract：:The Type I homodimeric photosynthetic reaction center found in anaerobic gram-positive bacteria of the genus Heliobacteriaceae incorporates FA- and FB-like iron-sulfur clusters similar to those found in Photosystem I as terminal electron acceptors. We recently isolated the PshB protein that harbors the iron-sulfur clu...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0622165

    authors： Heinnickel M,Shen G,Golbeck JH

    更新日期：2007-03-06 00:00:00

  • DNA hairpin structures in solution: 500-MHz two-dimensional 1H NMR studies on d(CGCCGCAGC) and d(CGCCGTAGC).

    abstract：:A hairpin structure contains two conformationally distinct domains: a double-helical stem with Watson-Crick base pairs and a single-stranded loop that connects the two arms of the stem. By extensive 1D and 2D 500-MHz 1H NMR studies in H2O and D2O, it has been demonstrated that the DNA oligomers d(CGCCGCAGC) and d(CGCC...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00398a027

    authors： Gupta G,Sarma MH,Sarma RH,Bald R,Engelke U,Oei SL,Gessner R,Erdmann VA

    更新日期：1987-12-01 00:00:00

  • Reversible self-association of bovine growth hormone during equilibrium unfolding.

    abstract：:Previous investigations have shown that bovine growth hormone (bGH, somatotropin) unfolds through a reversible multistate process with at least one stable equilibrium intermediate. In extending our knowledge of the folding process for bGH, we demonstrate that a self-associated form of partially denatured bGH is formed...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00369a029

    authors： Havel HA,Kauffman EW,Plaisted SM,Brems DN

    更新日期：1986-10-21 00:00:00

  • Sulphydryl group modification of aspartate aminotransferase with 3-bromo-1,1,1-trifluoropropanone during catalysis.

    abstract：:After protection of cysteine-45 and -82 with iodoacetamide or N-ethylmaleimide, and in the presence of saturating concentrations of substrates, the supernatant isozyme of pig heart aspartate transaminase has been covalently modified at cysteine-390 with 3-bromo-1,1,1-trifluoropropanone. The modified enzyme retains 60-...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00627a004

    authors： Critz WJ,Martinez-Carrion M

    更新日期：1977-04-19 00:00:00

  • An RNA polymerase mutant with reduced accuracy of chain elongation.

    abstract：:A new Escherichia coli RNA polymerase mutant was isolated which exhibited reduced accuracy of chain elongation in vivo and in vitro. The novel isolation procedure consisted of simultaneous selection for rifampicin resistance and screening for increased leakiness of an early, strongly polar nonsense mutation of lacZ, o...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00368a013

    authors： Blank A,Gallant JA,Burgess RR,Loeb LA

    更新日期：1986-10-07 00:00:00

  • A Modified ToxT Inhibitor Reduces Vibrio cholerae Virulence in Vivo.

    abstract：:We have previously designed and synthesized small-molecule inhibitors that reduce Vibrio cholerae virulence in vitro by targeting the transcription factor ToxT. Here we report the synthesis and biological activity of derivatives of our previous bicyclic, fatty acid-like inhibitors. All of the synthesized derivatives s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b00667

    authors： Woodbrey AK,Onyango EO,Kovacikova G,Kull FJ,Gribble GW

    更新日期：2018-09-25 00:00:00

  • Electrostatic effects in myoglobin. Application of the modified Tanford-Kirkwood theory to myoglobins from horse, California grey whale, harbor seal, and California sea lion.

    abstract：:The modified Tanford-Kirkwood electrostatic theory (Shire et al., 1974a) was applied to ferrimyoglobins from the following animal species: sperm whale (Physeter catodon), horse, California grey whale (Eschrichtius gibbosus), harbor seal (Phoca vitulina), and California sea lion (Zalophus californianus). Computations w...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00678a002

    authors： Shire SJ,Hanania GI,Gurd FR

    更新日期：1975-04-08 00:00:00

  • Carbon monoxide binding properties of hemoglobin M Iwate.

    abstract：:The kinetic and equilibrium CO binding properties of hemoglobin (Hb) M Iwate (alpha2 87 His leads to Tyr beta2) have been investigated. The results show that the alpha(Met) beta2 (CO) tetramer of this protein has a low affinity for CO, as indicated by the stopped-flow and flash-photolysis kinetic, as well as the CO bi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00669a022

    authors： Salhany JM,Castillo CL,Ogawa S

    更新日期：1976-11-30 00:00:00

  • Microtubule dynamic instability does not result from stabilization of microtubules by tubulin-GDP-Pi subunits.

    abstract：:The proposal that microtubule dynamic instability results from stabilization of microtubule ends by tubulin-GDP-Pi subunits (where Pi is inorganic phosphate) [Melki et al. (1996) Biochemistry 35, 12038] was based on studies of GTP hydrolysis and microtubule assembly that showed that tubulin-GDP-Pi subunits can transie...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi972602+

    authors： Caplow M,Shanks J

    更新日期：1998-09-15 00:00:00

  • Evidence for a singlet intermediate in catalysis by Escherichia coli DNA photolyase and evaluation of substrate binding determinants.

    abstract：:Escherichia coli DNA photolyase contains 1,5-dihydro-FAD (FADH2) plus 5,10-methenyl-tetrahydrofolate (5,10-CH+-H4folate). Both chromophores are fluorescent, and either can function as a sensitizer in catalysis. At 77 K separate fluorescence emission bands are observed for FADH2 (lambda max = 505 nm, shoulder at 540 nm...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00425a007

    authors： Jordan SP,Jorns MS

    更新日期：1988-12-13 00:00:00

  • Truncation of the amino terminus of human apolipoprotein A-I substantially alters only the lipid-free conformation.

    abstract：:An amino-terminal deletion mutant (residues 1-43) of human apolipoprotein A-I (apo hA-I) has been produced from a bacterial expression system to explore the structural and functional role of these amino acids, encoded by exon 3, in apo hA-I. Lipid binding of apo delta (1-43)A-I and lipid binding of apo hA-I are very s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi961876e

    authors： Rogers DP,Brouillette CG,Engler JA,Tendian SW,Roberts L,Mishra VK,Anantharamaiah GM,Lund-Katz S,Phillips MC,Ray MJ

    更新日期：1997-01-14 00:00:00

  • Inhibition of peptidyl-prolyl cis/trans isomerase activity by substrate analog structures: thioxo tetrapeptide-4-nitroanilides.

    abstract：:The ubiquitous cyclophilins belong to peptidyl-prolyl cis/trans isomerases (PPIases; EC 5.2.1.8). They are able to catalyze the cis/trans isomerization about peptidyl-prolyl amide bonds. The mode of action of human cytosolic cyclophilin (Cyp18cy) has been studied on substrate analog tetrapeptide-4-nitroanilides contai...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00040a012

    authors： Schutkowski M,Wöllner S,Fischer G

    更新日期：1995-10-10 00:00:00

  • Narrowing substrate specificity in a directly evolved enzyme: the A293D mutant of aspartate aminotransferase.

    abstract：:Several mutant Escherichia coli aspartate aminotransferases (eAATases) have been characterized in the attempt to evolve or rationally redesign the substrate specificity of eAATase into that of E. coli tyrosine aminotransferase (eTATase). These include HEX (designed), HEX + A293D (design followed by directed evolution)...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0487544

    authors： Chow MA,McElroy KE,Corbett KD,Berger JM,Kirsch JF

    更新日期：2004-10-12 00:00:00

  • Photosensitized inactivation of stem bromelain. Oxidation of histidine, methionine, and tryptophan residues.

    abstract：:Pineapple stem bromelain was photooxidized in the presence of Methylene Blue used as a sensitizer. The essential sulfhydryl group of the enzyme protein rapidly became inaccessible to react with 5,5'-dithiobis(nitrobenzoic acid), but the reactivity was readily regained to the original level upon treatment with dithioth...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00673a009

    authors： Murachi T,Tsudzuki T,Okumura K

    更新日期：1975-01-28 00:00:00

  • Molecular structure and polymorphic map of the human phenylalanine hydroxylase gene.

    abstract：:Human phenylalanine hydroxylase is a liver-specific enzyme that catalyzes the conversion of phenylalanine to tyrosine. Absence of enzymatic activity results in phenylketonuria, a genetic disorder that causes development of severe mental retardation in untreated children. In this paper we report the cloning and structu...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00352a001

    authors： DiLella AG,Kwok SC,Ledley FD,Marvit J,Woo SL

    更新日期：1986-02-25 00:00:00

  • Affinity cleavage at the metal-binding site of phosphoenolpyruvate carboxykinase.

    abstract：:Chicken liver phosphoenolpyruvate carboxykinase (PEPCK) was rapidly inactivated by micromolar concentrations of ferrous sulfate in the presence of ascorbate at pH 7.4. Omitting ascorbate or replacing the Fe2+ with Mn2+ or Mg2+ gives no inactivation. Mn2+, Mg2+, or Co2+ at 100-fold molar excess over Fe2+ offered comple...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi970574p

    authors： Hlavaty JJ,Nowak T

    更新日期：1997-12-09 00:00:00

  • Kinetics and processivity of ATP hydrolysis and DNA unwinding by the RecBC enzyme from Escherichia coli.

    abstract：:The RecB and RecC subunits of the RecBCD enzyme from Escherichia coli were purified from cells containing plasmids overproducing these proteins [Boehmer, P.E., & Emmerson, P.T. (1991) Gene 102, 1-6]. RecB hydrolyzes ATP in the presence of either single- or double-stranded DNA. RecC stimulates ATP hydrolysis by RecB, p...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00069a024

    authors： Korangy F,Julin DA

    更新日期：1993-05-11 00:00:00

  • Alternating zinc fingers in the human male-associated protein ZFY: HX3H and HX4H motifs encode a local structural switch.

    abstract：:The two-finger repeat in the human male-associated protein ZFY provides a model for comparative 2D-NMR studies of classical and variant Zn fingers. This repeat is defined in part by an alternation in spacing between consensus (HX3H) and variant (HX4H) histidine spacings. To investigate the effects of a "switch" betwee...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00103a002

    authors： Kochoyan M,Keutmann HT,Weiss MA

    更新日期：1991-10-01 00:00:00