当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Binding of a photoaffinity analogue of glutathione to MRP1 (ABCC1) within two cytoplasmic regions (L0 and L1) as well as transmembrane domains 10-11 and 16-17.

Binding of a photoaffinity analogue of glutathione to MRP1 (ABCC1) within two cytoplasmic regions (L0 and L1) as well as transmembrane domains 10-11 and 16-17.

Abstract：

:MRP1 (or ABCC1) is an ABC membrane protein that transports a wide range of natural products as well as glutathione (GSH)-, glucuronate-, and sulfate-conjugated metabolites. In addition, free GSH is required for MRP1 to transport several chemotherapeutic drugs. However, the mechanisms regulating the influence of GSH on MRP1 is poorly understood, and the location of GSH binding site(s) within MRP1 have yet to be determined. To address these issues, we have synthesized a [(125)I] labeled azido-derivative of GSH (IAAGSH) to photoaffinity label MRP1. Our results revealed that IAAGSH labeled MRP1 with high specificity, and binding was inhibited by MRP1 substrates leukotriene C(4) and MK571. Interestingly, verapamil and vincristine enhanced IAAGSH photolabeling of MRP1, in agreement with observations that both drugs enhance GSH transport. We observed GSH to be the best inhibitor of photoaffinity labeling, as compared to oxidized glutathione (GSSG) and four different GSH alkyl derivatives. These observations indicate that IAAGSH interacted with MRP1 in a similar manner as unmodified GSH. Moreover, using eight MRP1-HA variants, each containing hemagglutinin A (HA) epitopes inserted at different sites in MRP1, we mapped the GSH binding sites in MRP1. Our GSH analogue photoaffinity labeled four MRP1 polypeptides that were located within two cytoplasmic domains in linker sequences (L0 and L1) as well as transmembrane domains 10-11 and 16-17. The photoaffinity labeling of polypeptides within L0 and L1 domains is further confirmed using two MRP1-specific monoclonal antibodies (MRPr1 and QCRL1) with epitopes within the linker domains. Taken together, this study provides the most precise information to date on the location of GSH binding sites in MRP1.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Karwatsky J,Daoud R,Cai J,Gros P,Georges E

doi

10.1021/bi0268807

subject

Has Abstract

pub_date

2003-03-25 00:00:00

pages

3286-94

issue

11

eissn

0006-2960

issn

1520-4995

journal_volume

42

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Organization of the core lipids of lipoproteins from normal and cholesterol-fed rabbits. A proton nuclear magnetic resonance study.

    abstract：:When rabbits are fed a diet supplemented with cholesterol, their plasma cholesterol levels increase markedly, and they develop atherosclerosis. Most of the plasma cholesterol exists as cholesteryl esters in very low density lipoproteins (VLDL) and intermediate-density lipoproteins (IDL). The triglyceride content of th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00268a025

    authors： Kroon PA,Seidenberg J

    更新日期：1982-12-07 00:00:00

  • Phospholipids occupy the internal lumen of the c ring of the ATP synthase of Escherichia coli.

    abstract：:The occupancy of the central cavity of the membrane-embedded c ring of the ATP synthase of Escherichia coli was investigated with a photo-cross-linking approach. Single cysteine mutants were created at c subunit positions 4, 8, and 11, which are oriented to the inside of the ring. These cysteines were alkylated with r...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi052304+

    authors： Oberfeld B,Brunner J,Dimroth P

    更新日期：2006-02-14 00:00:00

  • Demonstration of the GC-rich common arm in yeast ribosomal 5.8S RNA via 500-MHz proton nuclear magnetic resonance and Overhauser enhancements.

    abstract：:In this paper we report the first 1H NMR study of the base-paired secondary structure of yeast 5.8S RNA. On the basis of a combination of homonuclear Overhauser enhancements and temperature dependence of the proton 500-MHz NMR spectrum, we are able to identify and assign eight of the nine base pairs in the most therma...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00373a018

    authors： Lee KM,Marshall AG

    更新日期：1986-12-16 00:00:00

  • Three-dimensional structure of the apo form of the N-terminal EGF-like module of blood coagulation factor X as determined by NMR spectroscopy and simulated folding.

    abstract：:The three-dimensional structure of a 42-residue fragment containing the N-terminal EGF-like module of blood coagulation factor X was determined by means of 2D NMR spectroscopy and computer simulation. The spectroscopic data consisted of 370 NOE distances and 27 dihedral angle constraints. These were used to generate p...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00141a004

    authors： Ullner M,Selander M,Persson E,Stenflo J,Drakenberg T,Teleman O

    更新日期：1992-07-07 00:00:00

  • Tryptophan 500 and arginine 707 define product and substrate active site binding in soybean lipoxygenase-1.

    abstract：:There is much debate whether the fatty acid substrate of lipoxygenase binds "carboxylate-end first" or "methyl-end first" in the active site of soybean lipoxygenase-1 (sLO-1). To address this issue, we investigated the sLO-1 mutants Trp500Leu, Trp500Phe, Lys260Leu, and Arg707Leu with steady-state and stopped-flow kine...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0489098

    authors： Ruddat VC,Mogul R,Chorny I,Chen C,Perrin N,Whitman S,Kenyon V,Jacobson MP,Bernasconi CF,Holman TR

    更新日期：2004-10-19 00:00:00

  • DNA dynamics in RecA-DNA filaments: ATP hydrolysis-related flexibility in DNA.

    abstract：:RecA-catalyzed DNA recombination is initiated by a mandatory, high-energy form of DNA in RecA-nucleoprotein filaments, where bases are highly unstacked and the backbone is highly unwound. Interestingly, only the energetics consequent to adenosine triphosphate (ATP) binding, rather than its hydrolysis, seems sufficient...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi034667k

    authors： Ramreddy T,Sen S,Rao BJ,Krishnamoorthy G

    更新日期：2003-10-21 00:00:00

  • Differences in secondary structure between packaged and unpackaged single-stranded DNA of bacteriophage phi X174 determined by Raman spectroscopy: a model for phi X174 DNA packaging.

    abstract：:The single-stranded packaged genome (ssDNA) of bacteriophage phi X174 is shown by Raman spectroscopy to lack both the ordered phosphodiester backbone and base stacking, which are demonstrated for unpackaged, protein-free ssDNA. In solutions of moderate ionic strength, unpackaged ssDNA contains 36 +/- 7% of deoxyribosy...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00234a004

    authors： Benevides JM,Stow PL,Ilag LL,Incardona NL,Thomas GJ Jr

    更新日期：1991-05-21 00:00:00

  • Influence of 5'-nearest neighbors on the insertion kinetics of the fluorescent nucleotide analog 2-aminopurine by Klenow fragment.

    abstract：:The effects of nearest neighbor interactions between a nucleotide base at the primer 3'-terminus and an incoming deoxyribonucleoside triphosphate on DNA polymerase catalyzed insertion were examined. Kinetics of inserting the fluorescent nucleotide analog 2-aminopurine deoxyribonucleotide (dAPMP) and dAMP opposite a te...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00092a039

    authors： Bloom LB,Otto MR,Beechem JM,Goodman MF

    更新日期：1993-10-19 00:00:00

  • Effects of temperature and viscosity on R67 dihydrofolate reductase catalysis.

    abstract：:R67 dihydrofolate reductase (DHFR) is a novel homotetrameric protein that possesses 222 symmetry and a single, voluminous active site pore. This symmetry poses numerous limitations on catalysis; for example, two dihydrofolate (DHF) molecules or two NADPH molecules, or one substrate plus one cofactor can bind. Only the...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi052504l

    authors： Chopra S,Lynch R,Kim SH,Jackson M,Howell EE

    更新日期：2006-05-30 00:00:00

  • Dynamics of ligand binding to alpha-chymotrypsin and to N-methyl-alpha-chymotrypsin.

    abstract：:Ks values for binding of selected substrates, competitive inhibitors, and a noncompetitive inhibitor were found to be similar for alpha-chymotrypsin and N-methyl-alpha-chymotrypsin. The rates and steps of binding of a competitive inhibitor and a noncompetitive inhibitor were also found to be similar for alpha-chymotry...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00262a017

    authors： Maehler R,Whitaker JR

    更新日期：1982-09-14 00:00:00

  • Calcium-induced structural transition in the regulatory domain of human cardiac troponin C.

    abstract：:While calcium binding to troponin C (TnC) triggers the contraction of both skeletal and cardiac muscle, there is clear evidence that different mechanisms may be involved. For example, activation of heart myofilaments occurs with binding to a single regulatory site on TnC, whereas activation of fast skeletal myofilamen...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi971223d

    authors： Spyracopoulos L,Li MX,Sia SK,Gagné SM,Chandra M,Solaro RJ,Sykes BD

    更新日期：1997-10-07 00:00:00

  • Human secreted carbonic anhydrase: cDNA cloning, nucleotide sequence, and hybridization histochemistry.

    abstract：:Complementary DNA clones coding for the human secreted carbonic anhydrase isozyme (CA VI) have been isolated and their nucleotide sequences determined. These clones identify a 1.45-kb mRNA that is present in high levels in parotid submandibular salivary glands but absent in other tissues such as the sublingual gland, ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00216a035

    authors： Aldred P,Fu P,Barrett G,Penschow JD,Wright RD,Coghlan JP,Fernley RT

    更新日期：1991-01-15 00:00:00

  • Structures of human carbonic anhydrase II/inhibitor complexes reveal a second binding site for steroidal and nonsteroidal inhibitors.

    abstract：:Carbonic anhydrase (CA) catalyzes the reversible hydration of carbon dioxide to hydrogen carbonate, and its role in maintaining pH balance has made it an attractive drug target. Steroidal sulfamate esters, inhibitors of the cancer drug target steroid sulfatase (STS), are sequestered in vivo by CA II in red blood cells...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi902178w

    authors： Cozier GE,Leese MP,Lloyd MD,Baker MD,Thiyagarajan N,Acharya KR,Potter BV

    更新日期：2010-04-27 00:00:00

  • In vitro conversion of formate to serine: effect of tetrahydropteroylpolyglutamates and serine hydroxymethyltransferase on the rate of 10-formyltetrahydrofolate synthetase.

    abstract：:Serine hydroxymethyltransferase and C1-tetrahydrofolate synthase catalyze four reactions which convert formate and glycine to serine. The one-carbon carrier in these reactions if tetrahydropteroylglutamate which is regenerated in the coupled reaction and thus can be used in catalytic concentrations with respect to ser...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00450a028

    authors： Strong WB,Schirch V

    更新日期：1989-11-28 00:00:00

  • 13C, 15N, and 31P NMR studies on 6-hydroxy-L-nicotine oxidase from Arthrobacter oxidans.

    abstract：:The interaction between the apoprotein of 6-hydroxy-L-nicotine oxidase from Arthrobacter oxidans and the prosthetic group FAD has been investigated by 13C, 15N, and 31P NMR techniques. The FAD prosthetic group was selectively enriched in 13C and 15N isotopes by adding isotopically labeled riboflavin derivatives to the...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00428a016

    authors： Pust S,Vervoort J,Decker K,Bacher A,Müller F

    更新日期：1989-01-24 00:00:00

  • Isolation and in vitro phosphorylation of sensory transduction components controlling anaerobic induction of light harvesting and reaction center gene expression in Rhodobacter capsulatus.

    abstract：:Anaerobic induction of light harvesting and reaction center gene expression involves two transacting factors termed RegA and RegB. Sequence and mutational analysis has indicated that RegA and RegB constitute cognate components of a prokaryotic sensory transduction cascade with RegB comprising a membrane-spanning senso...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00002a002

    authors： Inoue K,Kouadio JL,Mosley CS,Bauer CE

    更新日期：1995-01-17 00:00:00

  • Molten globule formation in apomyoglobin monitored by the fluorescent probe Nile Red.

    abstract：:The interaction of nile red (NR) with apomyoglobin (ApoMb) in the native (pH 7) and molten globule (pH 4) states was investigated using experimental and computational methods. NR binds to hydrophobic locations in ApoMb with higher affinity (K(d) = 25 +/- 5 microM) in the native state than in the molten globule state (...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi051905y

    authors： Polverini E,Cugini G,Annoni F,Abbruzzetti S,Viappiani C,Gensch T

    更新日期：2006-04-25 00:00:00

  • Trehalose-containing lipooligosaccharides of Mycobacterium gordonae: presence of a mono-O-methyltetra-O-acyltrehalose "core" and branching in the oligosaccharide backbone.

    abstract：:Past evidence has indicated that Mycobacterium gordonae, as isolated from soil and as an occasional opportunistic pathogen, exists as a serocomplex. We now demonstrate that the basis of seroreactivity and diversity is a novel series of alkali-labile, trehalose-containing lipooligosaccharides (LOS). The structures from...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00210a020

    authors： Besra GS,McNeil MR,Khoo KH,Dell A,Morris HR,Brennan PJ

    更新日期：1993-11-30 00:00:00

  • The newly discovered Parkinson's disease associated Finnish mutation (A53E) attenuates α-synuclein aggregation and membrane binding.

    abstract：:α-Synuclein (α-Syn) oligomerization and amyloid formation are associated with Parkinson's disease (PD) pathogenesis. Studying familial α-Syn mutants associated with early onset PD has therapeutic importance. Here we report the aggregation kinetics and other biophysical properties of a newly discovered PD associated Fi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi5010365

    authors： Ghosh D,Sahay S,Ranjan P,Salot S,Mohite GM,Singh PK,Dwivedi S,Carvalho E,Banerjee R,Kumar A,Maji SK

    更新日期：2014-10-21 00:00:00

  • Gonadotropin releasing hormone activates the lipoxygenase pathway in cultured pituitary cells: role in gonadotropin secretion and evidence for a novel autocrine/paracrine loop.

    abstract：:The formation and role of arachidonic acid (AA) and its metabolites during gonadotropin releasing hormone- (GnRH-) induced gonadotropin secretion were investigated in primary cultures of rat pituitary cells. Prelabeled cells ([3H]AA) responded to GnRH challenge with increased formation (about 2-fold) of the leukotrien...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00139a004

    authors： Dan-Cohen H,Sofer Y,Schwartzman ML,Natarajan RD,Nadler JL,Naor Z

    更新日期：1992-06-23 00:00:00

  • Evidence for the two phosphate binding sites of an analogue of the thioacyl intermediate for the Trypanosoma cruzi glyceraldehyde-3-phosphate dehydrogenase-catalyzed reaction, from its crystal structure.

    abstract：:Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of d-glyceraldehyde 3-phosphate (GAP) into d-glycerate 1,3-bisphosphate (1,3-diPG) in the presence of NAD(+) and inorganic phosphate (P(i)). Within the active site, two anion-binding sites were ascribed to the binding o...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0206107

    authors： Castilho MS,Pavão F,Oliva G,Ladame S,Willson M,Périé J

    更新日期：2003-06-17 00:00:00

  • A structure-based approach to a synthetic vaccine for HIV-1.

    abstract：:The generation of neutralizing antibodies by peptide immunization is dependent on achieving conformational compatibility between antibodies and native protein. Consequently, approaches are needed for developing conformational mimics of protein neutralization sites. We replace putative main-chain hydrogen bonds (NH -->...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0003691

    authors： Cabezas E,Wang M,Parren PW,Stanfield RL,Satterthwait AC

    更新日期：2000-11-28 00:00:00

  • Purified native subunits of bacterial luciferase are active in the bioluminescence reaction but fail to assemble into the alpha beta structure.

    abstract：:We have expressed the alpha and beta subunits of bacterial luciferase, encoded by luxA and luxB, from separate plasmids in Escherichia coli and developed an efficient purification scheme that yields many milligrams of protein of greater than 90% homogeneity. Earlier experiments showed that subunits synthesized separat...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00070a010

    authors： Sinclair JF,Waddle JJ,Waddill EF,Baldwin TO

    更新日期：1993-05-18 00:00:00

  • Characterization of Natural Colibactin-Nucleobase Adducts by Tandem Mass Spectrometry and Isotopic Labeling. Support for DNA Alkylation by Cyclopropane Ring Opening.

    abstract：:Colibactins are genotoxic secondary metabolites whose biosynthesis is encoded in the clb gene cluster harbored by certain strains of gut commensal Escherichia coli. Using synthetic colibactin analogues, we previously provided evidence that colibactins alkylate DNA by addition of a nucleotide to an electrophilic cyclop...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b01023

    authors： Xue M,Shine E,Wang W,Crawford JM,Herzon SB

    更新日期：2018-11-13 00:00:00

  • Comparative orientation of the fluorene residue in native DNA modified by N-acetoxy-N-2-acetylaminofluorene and two 7-halogeno derivatives.

    abstract：:Native calf thymus DNA was reacted with N-acetoxy-N-2-acetylaminofluorene (N-AcO-AAF) and its 7-fluoro and 7-iodo derivatives. Different ways of purification of the fluorene modified DNA samples were checked in order ot obtain a nucleic acid free from all noncovalently bound fluorene residues. The decrease in melting ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00660a027

    authors： Fuchs RP,Lefevre JF,Pouyet J,Daune MP

    更新日期：1976-07-27 00:00:00

  • Exonuclease-polymerase active site partitioning of primer-template DNA strands and equilibrium Mg2+ binding properties of bacteriophage T4 DNA polymerase.

    abstract：:The binding of bacteriophage T4 DNA polymerase (T4 pol) to primer-template DNA with 2-aminopurine (2AP) located at the primer terminus results in the formation of a hyperfluorescent 2AP state. Changes in this hyperfluorescent state were utilized to investigate the fractional concentration of primer-templates bound at ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi980074b

    authors： Beechem JM,Otto MR,Bloom LB,Eritja R,Reha-Krantz LJ,Goodman MF

    更新日期：1998-07-14 00:00:00

  • Rearrangement of domain elements of the Ca-ATPase in cardiac sarcoplasmic reticulum membranes upon phospholamban phosphorylation.

    abstract：:Phospholamban (PLB) is a major target of the beta-adrenergic cascade in the heart, and functions to modulate rate-limiting conformational transitions involving the transport activity of the Ca-ATPase. To investigate structural changes within the Ca-ATPase that result from the phosphorylation of PLB by cAMP-dependent p...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi990599j

    authors： Negash S,Huang S,Squier TC

    更新日期：1999-06-22 00:00:00

  • Nonequilibrium heating in LHCII complexes monitored by ultrafast absorbance transients.

    abstract：:Evidence of nonequilibrium local heating in transient spectra of LHCII, the main light-harvesting complex of plants, was studied by using various excitation intensities over a wide temperature range, from 10 K to room temperature. No obvious manifestation of local heating was found at room temperature, whereas at 10 K...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi060048a

    authors： Gulbinas V,Karpicz R,Garab G,Valkunas L

    更新日期：2006-08-08 00:00:00

  • Complex between a Multicrossover DNA Nanostructure, PX-DNA, and T7 Endonuclease I.

    abstract：:Paranemic crossover DNA (PX-DNA) is a four-stranded multicrossover structure that has been implicated in recombination-independent recognition of homology. Although existing evidence has suggested that PX is the DNA motif in homologous pairing (HP), this conclusion remains ambiguous. Further investigation is needed bu...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.9b00057

    authors： Kizer M,Huntress ID,Walcott BD,Fraser K,Bystroff C,Wang X

    更新日期：2019-03-12 00:00:00

  • DNA degradation by bleomycin: evidence for 2'R-proton abstraction and for C-O bond cleavage accompanying base propenal formation.

    abstract：:Reaction of poly(dA-[2'S-3H]dU) with activated bleomycin yields [3H]uracil propenal that completely retains the tritium label. In contrast, we have previously shown that reaction of poly(dA-[2'R-3H]dU) with activated bleomycin affords unlabeled uracil propenal [Wu, J. C., Kozarich, J. W., & Stubbe, J. (1983) J. Biol. ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00369a037

    authors： Ajmera S,Wu JC,Worth L Jr,Rabow LE,Stubbe J,Kozarich JW

    更新日期：1986-10-21 00:00:00