DNA dynamics in RecA-DNA filaments: ATP hydrolysis-related flexibility in DNA.

abstract：:Intracellular reduction of carcinogenic Cr(VI) generates Cr-DNA adducts formed through the coordination of Cr(III) to DNA phosphates (phosphotriester-type adduct). Here, we examined the role of Cr(III)-DNA adducts in mutagenesis induced by metabolism of Cr(VI) with cysteine. Reduction of Cr(VI) caused a strong oxidati...

journal_title：Biochemistry

authors： Zhitkovich A,Song Y,Quievryn G,Voitkun V

更新日期：2001-01-16 00:00:00

abstract：:Energy coupling factor (ECF) transporters take up micronutrients in Bacteria and Archaea. They consist of a membrane-embedded S-component that provides substrate specificity and a three-subunit ECF module that couples ATP hydrolysis to transport. The S-components ThiT (for thiamin) and NiaX (for niacin) from Lactococc...

journal_title：Biochemistry

authors： Majsnerowska M,Ter Beek J,Stanek WK,Duurkens RH,Slotboom DJ

更新日期：2015-08-11 00:00:00

abstract：:In the DNA recognition mode of C(2)H(2)-type zinc fingers, the finger-finger connection region, consisting of the histidine spacing (HX(3-5)H) and linker, would be important for determining the orientation of the zinc finger domains. To clarify the influence of spacing between two ligand histidines in the DNA binding,...

journal_title：Biochemistry

authors： Shiraishi Y,Imanishi M,Sugiura Y

更新日期：2004-05-25 00:00:00

abstract：:Myotoxin a from the venom of Crotalus viridis viridis (prairie rattlesnake) is a small protein which is responsible for myonecrosis. It is a basic protein with 42 amino acid residues of known sequence. Three disulfide bonds give it a highly compact structure. Microscopic examination of the toxin's effects reveals that...

journal_title：Biochemistry

authors： Liddle WK,Tu AT

更新日期：1985-12-17 00:00:00

abstract：:Apolipoprotein AIV is one of the principal apolipoproteins synthesized by the rat small intestine. We have cloned a cDNA encoding a portion of preapolipoprotein AIV mRNA. A kinetically fractionated cDNA probe highly enriched for the abundant intestinal mucosal mRNA sequences was used to screen a library of recombinant...

journal_title：Biochemistry

authors： Gordon JI,Smith DP,Alpers DH,Strauss AW

更新日期：1982-10-26 00:00:00

abstract：:Rapid withdrawal of estrogen from immature chicks, previously stimulated with the hormone, results in the inhibition of transcription of mRNAs of egg white proteins, rapid degradation of existing estrogen-induced mRNAs of egg white proteins, and decline in ribosomes and weight of the oviduct. On rapid withdrawal of es...

journal_title：Biochemistry

authors： Cohrs RJ,Goswami BB,Sharma OK

更新日期：1988-05-03 00:00:00

abstract：:Members of the phospholipase C-beta (PLC-beta) family of proteins are activated either by G alpha or G beta gamma subunits of heterotrimeric G proteins. To define specific regions of PLC-beta 3 that are involved in binding and activation by G beta gamma, a series of fragments of PLC-beta 3 as glutathione-S-transferase...

journal_title：Biochemistry

authors： Barr AJ,Ali H,Haribabu B,Snyderman R,Smrcka AV

更新日期：2000-02-22 00:00:00

abstract：:The carbohydrate binding specificity of the seed lectin from Artocarpus integrifolia, artocarpin, has been elucidated by the enzyme-linked lectin absorbent assay [Misquith, S., et al (1994) J. Biol. Chem. 269, 30393-30401], wherein it was demonstrated to be a Man/Glc specific lectin with high affinity for the trisacch...

journal_title：Biochemistry

authors： Rani PG,Bachhawat K,Reddy GB,Oscarson S,Surolia A

更新日期：2000-09-05 00:00:00

abstract：:P1,P2-Bidentate Co(NH3)4PP was found to be a competitive inhibitor of pyrophosphatase vs. MgPP (Kis = 8.7 mM, pH 7) and, in the presence of Mg2+, an active substrate as well. P1,P2-Bidentate Cr(III) complexes of pyrophosphate, imidodiphosphate, and methylenediphosphonate were also competitive inhibitors vs. MgPP (pH 5...

journal_title：Biochemistry

authors： Knight WB,Fitts SW,Dunaway-Mariano D

更新日期：1981-07-07 00:00:00

abstract：:Thioacetamide, a hepatocarcinogen and an inhibitor of heme synthesis, blocks the phenobarbitone-mediated increase in the transcription of cytochrome P-450b+e messenger RNA in rat liver. This property is also shared by CoCl2 and 3-amino-1,2,4-triazole, two other inhibitors of heme synthesis. Thus, it appears feasible t...

journal_title：Biochemistry

authors： Satyabhama S,Seelan RS,Padmanaban G

更新日期：1986-08-12 00:00:00

abstract：:Ornithine decarboxylase (ODC) is a pyridoxal 5'-phosphate (PLP) dependent homodimeric enzyme. It is a recognized drug target against African sleeping sickness, caused by Trypanosoma brucei. One of the currently used drugs, alpha-difluoromethylornithine (DFMO), is a suicide inhibitor of ODC. The structure of the T. bru...

journal_title：Biochemistry

authors： Grishin NV,Osterman AL,Brooks HB,Phillips MA,Goldsmith EJ

更新日期：1999-11-16 00:00:00

abstract：:For many different enveloped viruses the crystal structure of the fusion protein core has been established. A striking conservation in the tertiary and quaternary arrangement of these core structures is repeatedly revealed among members of diverse families. It has been proposed that the primary role of the core involv...

journal_title：Biochemistry

authors： Sackett K,Shai Y

更新日期：2002-04-09 00:00:00

abstract：:The structure of a short-chain IgG2a antibody, which is a member of the family of mouse anti-dansyl switch variant antibodies with identical variable regions but different heavy-chain constant regions [Dangl, J.L., Parks, D. R., Oi, V. T., & Herzenberg, L. A. (1982) Cytometry 2, 395-401], is reported. Amino acid seque...

journal_title：Biochemistry

authors： Igarashi T,Sato M,Katsube Y,Takio K,Tanaka T,Nakanishi M,Arata Y

更新日期：1990-06-19 00:00:00

abstract：:Bacillus thuringiensis (Bt) insecticidal toxins bind to receptors on midgut epithelial cells of susceptible insects, and binding triggers biochemical events that lead to insect mortality. Recently, a 100-kDa aminopeptidase N (APN) was isolated from brush border membrane vesicles (BBMV) of Anopheles quadrimaculatus and...

journal_title：Biochemistry

authors： Zhang R,Hua G,Andacht TM,Adang MJ

更新日期：2008-10-28 00:00:00

abstract：:Protein-protein interactions such as those between small G proteins and their effector proteins control most cell signaling pathways and thereby govern many cellular processes in both normal and disease states. Each small G protein interacts with several effectors, some shared between similar G proteins and others uni...

journal_title：Biochemistry

authors： Elliot-Smith AE,Owen D,Mott HR,Lowe PN

更新日期：2007-12-11 00:00:00

abstract：:In experiments designed to characterize the basis of amyloid fibril stability through mutational analysis of the Abeta (1-40) molecule, fibrils exhibit consistent, significant structural malleability. In these results, and in other properties, amyloid fibrils appear to more resemble plastic materials generated from sy...

journal_title：Biochemistry

authors： Wetzel R,Shivaprasad S,Williams AD

更新日期：2007-01-09 00:00:00

abstract：:Catalysis by glycogen phosphorylase involves a mechanism in which binding of one substrate tightens the binding of the other substrate to produce a productive ternary enzyme-substrate complex. In this work the molecular basis for this synergism is probed in crystallographic studies on ternary complexes in which the gl...

journal_title：Biochemistry

authors： Mitchell EP,Withers SG,Ermert P,Vasella AT,Garman EF,Oikonomakos NG,Johnson LN

更新日期：1996-06-11 00:00:00

abstract：:The annexin fold consists of four 70-residue domains with markedly homologous sequences and nearly identical structures. Each domain contains five helices designated A to E. Domain 2 of annexin I was obtained by chemical synthesis including ten specifically labeled residues and studied by 1H-15N NMR and circular dichr...

journal_title：Biochemistry

authors： Cordier-Ochsenbein F,Guerois R,Baleux F,Huynh-Dinh T,Chaffotte A,Neumann JM,Sanson A

更新日期：1996-08-13 00:00:00

abstract：:We have established that UvrABC nuclease is equally efficient in cutting mitomycin C (MC)-DNA monoadducts formed at different sequences and that the degree of UvrABC cutting represents the extent of drug-DNA bonding. Using this method we determined the effect of C5 cytosine methylation on the DNA monoalkylation by MC ...

journal_title：Biochemistry

authors： Li VS,Reed M,Zheng Y,Kohn H,Tang M

更新日期：2000-03-14 00:00:00

abstract：:Mammalian cGMP- and cAMP-dependent protein kinase show considerable similarity in amino acid sequence, although they specifically bind different cyclic nucleotides. Results of cGMP analogue binding experiments, combined with modeling of the cGMP binding sites by analogy to the structure of the homologous catabolite ge...

journal_title：Biochemistry

authors： Weber IT,Shabb JB,Corbin JD

更新日期：1989-07-11 00:00:00

abstract：:Studies utilizing the overexpression of individual isoforms indicated that both PKC-alpha and -delta promote a number of biological effects, including inhibition of DNA synthesis associated with rearrangements of the actin cytoskeleton in the murine B-cell lymphoma (Baf3), differentiation of the murine promyelocyte li...

journal_title：Biochemistry

authors： Romanova LY,Alexandrov IA,Nordan RP,Blagosklonny MV,Mushinski JF

更新日期：1998-04-21 00:00:00

abstract：:Piscidin 4, an antimicrobial peptide recently isolated from mast cells of hybrid striped bass (Morone chrysops female × Morone saxatilis male), is unusual in that it is twice as long (44 amino acids) as the typical members of the piscidin family. We previously showed that native piscidin 4 had a modified amino acid at...

journal_title：Biochemistry

authors： Park NG,Silphaduang U,Moon HS,Seo JK,Corrales J,Noga EJ

更新日期：2011-04-26 00:00:00

abstract：:The molecular structure of ammonium deoxycytidylyl-(3'-5')-deoxyguanosine, crystallized from aqueous acetone near pH 4, was determined for X-ray diffraction data. The crystals were tetragonal, space group P43212 with a = b = 11.078 (1) A and c = 45.826 (4) A. The structure was solved by tangent expansion of phases bas...

journal_title：Biochemistry

authors： Cruse WB,Egert E,Kennard O,Sala GB,Salisbury SA,Viswamitra MA

更新日期：1983-04-12 00:00:00

abstract：:Thermostable direct hemolysin (TDH) of Vibrio parahemolyticus is a membrane-damaging pore-forming toxin with potent cytolytic/cytotoxic activity. TDH exists as a tetramer consisting of protomers with a core β-sandwich domain, flanked by an 11-amino acid long N-terminal region (NTR). This NTR could not be modeled in th...

journal_title：Biochemistry

authors： Kundu N,Verma P,Kumar A,Dhar V,Dutta S,Chattopadhyay K

更新日期：2020-02-04 00:00:00

abstract：:The mobility of vesicular stomatitis virus (VSV) G protein on the surface of infected BHK cells was studied by using the technique of fluorescence photobleaching recovery. The fraction of surface G protein that was mobile in that time scale of the measurement (minutes) was at least 75%, a relatively high value among c...

journal_title：Biochemistry

authors： Reidler JA,Keller PM,Elson EL,Lenard J

更新日期：1981-03-03 00:00:00

abstract：:Ketosteroid isomerase (KSI) is one of the most proficient enzymes catalyzing an allylic isomerization reaction at a diffusion-controlled rate. In this study of KSI, we have detailed the structures of its active site, the role of various catalytic residues, and have explained the origin of the its fast reactivity by ca...

journal_title：Biochemistry

authors： Oh KS,Cha SS,Kim DH,Cho HS,Ha NC,Choi G,Lee JY,Tarakeshwar P,Son HS,Choi KY,Oh BH,Kim KS

更新日期：2000-11-14 00:00:00

abstract：:The calcitonin/CGRP gene pre-mRNA is alternatively spliced in a tissue-specific manner resulting in the formation of calcitonin mRNA in thyroid C-cells and CGRP mRNA in neurons. Computer analysis of the RNA containing the 3' splice acceptor of the calcitonin-specific exon 4 predicts that this region has the potential ...

journal_title：Biochemistry

authors： Coleman TP,Roesser JR

更新日期：1998-11-10 00:00:00

abstract：:The heterodimeric actin capping protein (CP) is regulated by a set of proteins that contain CP-interacting (CPI) motifs. Outside of the CPI motif, the sequences of these proteins are unrelated and distinct. The CPI motif and surrounding sequences are conserved within a given protein family, when compared to those of o...

journal_title：Biochemistry

authors： McConnell P,Mekel M,Kozlov AG,Mooren OL,Lohman TM,Cooper JA

更新日期：2020-03-24 00:00:00

abstract：:The DNA strand cleavage reaction catalyzed by endonuclease III from Escherichia coli (endo III) on the 3'-side of aldehyde abasic sites proceeds by a syn beta-elimination involving abstraction of the 2'-pro-S proton and formation of a trans alpha,beta-unsaturated aldose product; we previously reported the same stereoc...

journal_title：Biochemistry

authors： Mazumder A,Gerlt JA,Absalon MJ,Stubbe J,Cunningham RP,Withka J,Bolton PH

更新日期：1991-01-29 00:00:00

abstract：:Insulin-stimulated glucose disposal in skeletal muscle proceeds predominantly through a nonoxidative pathway with glycogen synthase as a rate-limiting enzyme, yet the mechanisms for insulin activation of glycogen synthase are not understood despite years of investigation. Isolation of putative insulin second messenger...

journal_title：Biochemistry

authors： Brautigan DL,Brown M,Grindrod S,Chinigo G,Kruszewski A,Lukasik SM,Bushweller JH,Horal M,Keller S,Tamura S,Heimark DB,Price J,Larner AN,Larner J

更新日期：2005-08-23 00:00:00