Gonadotropin releasing hormone activates the lipoxygenase pathway in cultured pituitary cells: role in gonadotropin secretion and evidence for a novel autocrine/paracrine loop.

abstract：:Metal homeostasis and resistance in bacteria is maintained by a panel of metal-sensing transcriptional regulators that collectively control transition metal availability and mediate resistance to heavy metal xenobiotics, including As(III), Cd(II), Pb(II), and Hg(II). The ArsR family constitutes a superfamily of metal ...

journal_title：Biochemistry

authors： Wang Y,Kendall J,Cavet JS,Giedroc DP

更新日期：2010-08-10 00:00:00

abstract：:The assignment of the 1H, 15N, 13CO, and 13C resonances of recombinant human interleukin-4 (IL-4), a protein of 133 residues and molecular mass of 15.4 kDa, is presented based on a series of 11 three-dimensional (3D) double- and triple-resonance heteronuclear NMR experiments. These studies employ uniformly labeled 15N...

journal_title：Biochemistry

authors： Powers R,Garrett DS,March CJ,Frieden EA,Gronenborn AM,Clore GM

更新日期：1992-05-05 00:00:00

abstract：:Since a major function of RNA polymerase must be to bring together substrates in the optimal configuration for internucleotide bond formation, studies have been undertaken to understand the geometrical relationship of the two substrates. A model has been constructed for the geometry of interaction of two ATP molecules...

journal_title：Biochemistry

authors： Beal RB,Pillai RP,Chuknyisky PP,Levy A,Tarien E,Eichhorn GL

更新日期：1990-06-26 00:00:00

abstract：:X-ray crystallographic studies have shown that DNA binding by human polymerase beta (pol beta) occurs primarily through two structurally and sequentially homologous helix-hairpin-helix (HhH) motifs, one in the fingers subdomain and the other in the 8-kDa domain [Pelletier, H., Sawaya, M. R., Wolfle, W., Wilson, S. H.,...

journal_title：Biochemistry

authors： Pelletier H,Sawaya MR

更新日期：1996-10-01 00:00:00

abstract：:2,3-Butanedione monoxime (BDM) reversibly inhibits force production in muscle. At least part of its action appears to be directly on the contractile apparatus. To understand better its mechanism of action, we studied the effect of BDM on the steps of myosin subfragment 1 Mg(2+)-ATPase in 0.1 M potassium acetate, pH 7....

journal_title：Biochemistry

authors： Herrmann C,Wray J,Travers F,Barman T

更新日期：1992-12-08 00:00:00

abstract：:The chemical mechanism of the phosphoribosyltransferases (PRTases), although largely unknown, may proceed either via a concerted direct-transfer mechanism or with a two-step mechanism involving a carboxonium-like intermediate. To study this question, we have cloned the Salmonella typhimurium pyrE gene, coding for the ...

journal_title：Biochemistry

authors： Bhatia MB,Vinitsky A,Grubmeyer C

更新日期：1990-11-20 00:00:00

abstract：:Ethylene and its analogues acetylene, carbon monoxide, and propylene inhibited the rate of oxidation of indole-3-acetic acid by peroxidase. Annulment of this effect by addition of superoxide dismutase showed that inhibition occurred only in the presence of the superoxide anion radical (O2-.). Kinetic and spectral data...

journal_title：Biochemistry

authors： Smith AM,Morrison WL,Milham PJ

更新日期：1983-03-29 00:00:00

abstract：:Kinetic parameters for triosephosphate isomerase catalysis of the elimination reaction of an equilibrium mixture of dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (DGAP) to form methylglyoxal and phosphate ion are reported for the enzyme from rabbit muscle. Pseudo-first-order rate constants for the...

journal_title：Biochemistry

authors： Richard JP

更新日期：1991-05-07 00:00:00

abstract：:Sendai virus particles are able to fuse with Pronase-neuraminidase-treated human erythrocyte membranes as well as with vesicles obtained from chromaffin granules of bovine medulla. Fusion is inferred either from electron microscopic studies or from the observation that incubation of fluorescently labeled (bearing octa...

journal_title：Biochemistry

authors： Citovsky V,Laster Y,Schuldiner S,Loyter A

更新日期：1987-06-30 00:00:00

abstract：:An A81H variant of yeast iso-1-cytochrome c is prepared to test the hypothesis that the steric size of the amino acid at sequence position 81 of cytochrome c, which has evolved from Ala in yeast to Ile in mammals, slows the dynamics of the opening of the heme crevice. The A81H mutation is used both to increase steric ...

journal_title：Biochemistry

authors： Bandi S,Bowler BE

更新日期：2015-03-10 00:00:00

abstract：:We have investigated the interactions of the p21 protein of c-Ha ras with its phospholipid environment. Gel filtration of detergent-"solubilized" p21 revealed that this preparation consisted of a mixture of multimolecular aggregates of protein and phospholipid and also a population of individual p21 molecules. Additio...

journal_title：Biochemistry

authors： Montgomery GW,Jagger BA,Bailey PD

更新日期：1988-06-14 00:00:00

abstract：:The precipitation mechanism of alpha-globulin in the presence of myo-inositol hexaphosphate (sodium phytate) was studied in detail. The maximum interaction was found at pH 2.3 where the protein was in a dissociated state having an 8.3S aggregate and a 1.5S monomer. This interaction was predominantly dependent upon the...

journal_title：Biochemistry

authors： Rajendran S,Prakash V

更新日期：1993-04-06 00:00:00

abstract：:The cyclic antimicrobial lipopeptide daptomycin is now frequently used as a first-line therapy in serious infections caused by multidrug-resistant Enterococcus faecium. Resistance to daptomycin in E. faecium is mediated by activation of the LiaFSR membrane stress response pathway. Deletion of liaR, encoding the respon...

journal_title：Biochemistry

authors： Davlieva M,Wu C,Zhou Y,Arias CA,Shamoo Y

更新日期：2018-12-11 00:00:00

abstract：:Complete proton assignments are reported for the 1H nuclear magnetic resonance (NMR) spectrum of Escherichia coli thioredoxin in the oxidized (with active-site disulfide bridge) and reduced (with two sulfhydryl groups) states. The assignments were obtained by using an integrated assignment strategy in which spin syste...

journal_title：Biochemistry

authors： Dyson HJ,Holmgren A,Wright PE

更新日期：1989-08-22 00:00:00

abstract：:Sarcoplasmic phosphorylase phosphatase extracted from ground skeletal muscle was recovered in a high molecular weight from (Mr = 250000). This enzyme has been purified from extracts by anion-exchange and gel chromatography to yield a preparation with three major protein components of Mr 83000, 72000, and 32000 by sodi...

journal_title：Biochemistry

authors： Brautigan DL,Picton C,Fischer EH

更新日期：1980-12-09 00:00:00

abstract：:Diethyl pyrocarbonate inhibited diaphorase activity of ferredoxin-NADP+ oxidoreductase with a second-order rate constant of 2 mM-1 X min-1 at pH 7.0 and 20 degrees C, showing a concomitant increase in absorbance at 242 nm due to formation of carbethoxyhistidyl derivatives. Activity could be restored by hydroxylamine, ...

journal_title：Biochemistry

authors： Carrillo N,Vallejos RH

更新日期：1983-12-06 00:00:00

abstract：:It was previously observed that two steps of the reaction of eukaryotic DNA topoisomerase I (topoisomerization and cleavage) depend upon the conformation of the DNA substrate: in both instances the supercoiled form is a more efficient substrate than the relaxed one. This paper reports the analysis of two other steps o...

journal_title：Biochemistry

authors： Caserta M,Amadei A,Camilloni G,Di Mauro E

更新日期：1990-09-04 00:00:00

abstract：:Class A beta-lactamases hydrolyze penicillins and other beta-lactams via an acyl-enzyme catalytic mechanism. Ser70 is the active site nucleophile. By constructing the S70A mutant, which is unable to form the acyl-enzyme intermediate, it was possible to make stable ES complexes with various substrates. The stability of...

journal_title：Biochemistry

authors： Hokenson MJ,Cope GA,Lewis ER,Oberg KA,Fink AL

更新日期：2000-05-30 00:00:00

abstract：:Thiazolinyl imine reductases catalyze the NADPH-dependent reduction of a thiazoline to a thiazolidine, a required step in the formation of the siderophores yersiniabactin (Yersinia spp.) and pyochelin (Pseudomonas aeruginosa). These stand-alone nonribosomal peptide tailoring domains are structural homologues of sugar ...

journal_title：Biochemistry

authors： Meneely KM,Ronnebaum TA,Riley AP,Prisinzano TE,Lamb AL

更新日期：2016-09-27 00:00:00

abstract：:Weak or nonexistent smallpox immunity in today's human population raises concerns about the possibility of natural or provoked genetic modifications leading to re-emergence of variola virus and other poxviruses. Thus, the development of new antiviral strategies aimed at poxvirus infections in humans is a high priority...

journal_title：Biochemistry

authors： Duraffour S,Ishchenko AA,Saparbaev M,Crance JM,Garin D

更新日期：2007-10-23 00:00:00

abstract：:Phenylalanine hydroxylase (PheH) and tryptophan hydroxylase (TrpH) catalyze the aromatic hydroxylation of phenylalanine and tryptophan, forming tyrosine and 5-hydroxytryptophan, respectively. The reactions of PheH and TrpH have been investigated with [4-(2)H]-, [3,5-(2)H(2)]-, and (2)H(5)-phenylalanine as substrates. ...

journal_title：Biochemistry

authors： Pavon JA,Fitzpatrick PF

更新日期：2006-09-12 00:00:00

abstract：:Restrictocin and related fungal endoribonucleases from the α-sarcin family site-specifically cleave the sarcin/ricin loop (SRL) on the ribosome to inhibit translation and ultimately trigger cell death. Previous studies showed that the SRL folds into a bulged-G motif and tetraloop, with restrictocin achieving a specifi...

journal_title：Biochemistry

authors： Plantinga MJ,Korennykh AV,Piccirilli JA,Correll CC

更新日期：2011-04-12 00:00:00

abstract：:The composition of the molybdate-stabilized glucocorticoid receptor (GR) complex has been investigated with a monoclonal antibody against the steroid-binding Mr 94 000 (94K) GR protein. It was concluded that one antibody molecule binds one 94K GR molecule. This finding constituted the basis for calculating the number ...

journal_title：Biochemistry

authors： Okret S,Wikström AC,Gustafsson JA

更新日期：1985-11-05 00:00:00

abstract：:The expression of the transforming gene product of Rous sarcoma virus (pp60v-src) in Saccharomyces cerevisiae has recently been reported (Kornbluth et al., 1987; Brugge et al., 1987). To carry out biochemical and structural studies of this enzyme, a facile purification was developed. The purification was accomplished ...

journal_title：Biochemistry

authors： Radziejewski C,Miller WT,Mobashery S,Goldberg AR,Kaiser ET

更新日期：1989-11-14 00:00:00

abstract：:Mutation of Pro94 to phenylalanine or alanine significantly alters the redox properties of the type I copper center of amicyanin. Each mutation increases the redox midpoint potential (E(m)) value by at least 140 mV and shifts the pK(a) for the pH dependence of the E(m) value to a more acidic value. Atomic resolution (...

journal_title：Biochemistry

authors： Carrell CJ,Sun D,Jiang S,Davidson VL,Mathews FS

更新日期：2004-07-27 00:00:00

abstract：:A detailed kinetic analysis of the catalytic trimer of aspartate transcarbamoylase containing the active site substitution H134A was performed to investigate the role of His 134 in the catalytic mechanism. Replacement of histidine by alanine resulted in decreases in the affinities for the two substrates, carbamoyl pho...

journal_title：Biochemistry

authors： Waldrop GL,Turnbull JL,Parmentier LE,O'Leary MH,Cleland WW,Schachman HK

更新日期：1992-07-21 00:00:00

abstract：:M13 DNA containing 20-30 apurinic/apyrimidinic (AP) sites per intact circular molecule was prepared by growing phage on an ung- dut- Escherichia coli mutant and treating the DNA with uracil N-glycosylase. AP sites obstruct in vitro DNA synthesis catalyzed by E. coli pol I. The position at which termination of synthesi...

journal_title：Biochemistry

authors： Sagher D,Strauss B

更新日期：1983-09-13 00:00:00

abstract：:Iron is vital for almost all organisms because of its ability to donate and accept electrons with relative ease. It serves as a cofactor for many proteins and enzymes necessary for oxygen and energy metabolism, as well as for several other essential processes. Mammalian cells utilize multiple mechanisms to acquire iro...

journal_title：Biochemistry

authors： Pantopoulos K,Porwal SK,Tartakoff A,Devireddy L

更新日期：2012-07-24 00:00:00

abstract：:The class C serine beta-lactamase of Enterobacter cloacae P99 was inhibited by a series of aryl methylphosphonate monoester monoanions. The effectiveness of these inhibitors was promoted by an acylamido substituent on the methyl group and a good leaving group at phosphorus. The former preference suggests that noncoval...

journal_title：Biochemistry

authors： Rahil J,Pratt RF

更新日期：1992-06-30 00:00:00

abstract：:Experiments were performed to elucidate the mechanism of hydrocarbon formation in microsomal preparations from the house fly, Musca domestica. Antibody to both house fly cytochrome P450 reductase and a purified cytochrome P450 (CYP6A1) from the house fly inhibited (Z)-9-tricosene (Z9-23:Hy) formation from [15,16-3H]-(...

journal_title：Biochemistry

authors： Reed JR,Quilici DR,Blomquist GJ,Reitz RC

更新日期：1995-12-12 00:00:00