Normal cellular Ha ras p21 protein causes local disruption of bilayer phospholipid.

abstract：:Mitomycins make up a group of antitumor natural products that are biosynthesized from aminohydroxybenzoic acid (AHBA) and N-acetylglucosamine (GlcNAc). While the biosynthetic gene cluster was reported two decades ago, the mechanism by which the two building blocks, AHBA and GlcNAc, are coupled during biosynthesis rema...

journal_title：Biochemistry

authors： Nguyen HP,Yokoyama K

更新日期：2019-06-25 00:00:00

abstract：:The biodegradative 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase of Pseudomonas mevalonii catalyzes the NAD(+)-dependent conversion of (S)-HMG-CoA to (R)-mevalonate. Crystallographic analysis of abortive ternary complexes of this enzyme revealed lysine 267 located at a position in the active site, suggesti...

journal_title：Biochemistry

authors： Bochar DA,Tabernero L,Stauffacher CV,Rodwell VW

更新日期：1999-07-13 00:00:00

abstract：:The temperature dependence of steady-state kinetics has been studied with horse liver alcohol dehydrogenase (HLADH) using protonated and deuterated benzyl alcohol as substrates in methanol/water mixtures between +3 and -50 degrees C. Additionally, the competitive isotope effects, k(H)/k(T) and k(D)/k(T), were measured...

journal_title：Biochemistry

authors： Tsai S,Klinman JP

更新日期：2001-02-20 00:00:00

abstract：:A T203Y substitution in green fluorescent protein causes a red shift in emission to yield a class of mutants known as yellow fluorescent protein (YFP). Many of these YFP mutants bind halides with affinities in the millimolar range, which often results in the chromophore pK values being shifted into the physiological r...

journal_title：Biochemistry

authors： Seward HE,Basran J,Denton R,Pfuhl M,Muskett FW,Bagshaw CR

更新日期：2013-04-09 00:00:00

abstract：:Current antimitotic cancer chemotherapy based on vinca alkaloids and taxanes target tubulin, a protein required not only for mitotic spindle formation but also for the overall structural integrity of terminally differentiated cells. Among many innovations targeting specific mitotic events, inhibition of motor enzymes ...

journal_title：Biochemistry

authors： Sheth PR,Basso A,Duca JS,Lesburg CA,Ogas P,Gray K,Nale L,Mannarino AF,Prongay AJ,Le HV

更新日期：2009-11-24 00:00:00

abstract：:trans-3-Chloroacrylic acid dehalogenase (CaaD) converts trans-3-chloroacrylic acid to malonate semialdehyde by the addition of H(2)O to the C-2, C-3 double bond, followed by the loss of HCl from the C-3 position. Sequence similarity between CaaD, an (alphabeta)(3) heterohexamer (molecular weight 47,547), and 4-oxalocr...

journal_title：Biochemistry

authors： Azurmendi HF,Wang SC,Massiah MA,Poelarends GJ,Whitman CP,Mildvan AS

更新日期：2004-04-13 00:00:00

abstract：:The purified ATP-synthesizing complex from chloroplasts has been reconstituted into phospholipid vesicles with bacteriorhodopsin by use of octyl glucoside. Phosphorylation rates up to 90 mmol of ATP (mg of protein)-1 min-1 have been achieved. The dependence of the steady-state kinetic parameters on external and intern...

journal_title：Biochemistry

authors： Takabe T,Hammes GG

更新日期：1981-11-24 00:00:00

abstract：:Mutations in the Kirsten ras (KRAS) gene are present in almost all pancreatic adenocarcinomas, and one common mutation is at codon 12: GGT (Gly) is transformed into GAT (Asp). In this work we have targeted the KRAS coding sequence embracing the GAT mutation with a sense PNA molecule (P14), with the aim of downregulati...

journal_title：Biochemistry

authors： Cogoi S,Codognotto A,Rapozzi V,Meeuwenoord N,van der Marel G,Xodo LE

更新日期：2005-08-09 00:00:00

abstract：:The ATPase activity of the (Ca2+-Mg2+)-ATPase reconstituted into bilayers of phosphatidylcholines depends on the fatty acyl chain length of the phospholipids. It is shown that the fluorescence response to Ca2+ of the ATPase modified with fluorescein isothiocyanate is also dependent on phospholipid structure and is int...

journal_title：Biochemistry

authors： Froud RJ,East JM,Jones OT,Lee AG

更新日期：1986-11-18 00:00:00

abstract：:Ion channel peptides have been prepared by solid-phase peptide synthesis, labeled with 15N at selected sites, and reconstituted into oriented lipid bilayers. The (Leu-Ser-Ser-Leu-Leu-Ser-Leu)3-CONH2 peptide has previously been shown to exhibit well-defined and discrete ionic conductances when investigated by single-ch...

journal_title：Biochemistry

authors： Sudheendra US,Bechinger B

更新日期：2005-09-13 00:00:00

abstract：:βγ-Crystallins are important constituents of the vertebrate eye lens, whereas in microbes, they are prevalent as Ca2+-binding proteins. In archaea, βγ-crystallins are conspicuously confined to two methanogens, viz., Methanosaeta and Methanosarcina. One of these, i.e., M-crystallin from Methanosarcina acetivorans, has ...

journal_title：Biochemistry

authors： Srivastava SS,Jamkhindikar AA,Raman R,Jobby MK,Chadalawada S,Sankaranarayanan R,Sharma Y

更新日期：2017-03-07 00:00:00

abstract：:The following polynucleotides containing the antibiotic tubercidin (Tu; 4-amino-7-beta-D-ribofuranosylpyrrolo-[2,3-d]pyrimidine) were enzymatically synthesized by polymerization of adenosine 5'-diphosphate-tubercidin 5'-diphosphate mixtures with polynucleotide phosphorylase: poly(A2,Tu), poly(A,Tu2), and poly(Tu). The...

journal_title：Biochemistry

authors： Seela F,Tran TQ,Mentzel H,Erdmann VA

更新日期：1981-04-28 00:00:00

abstract：:Endothelium-derived epoxyeicosatrienoic acids (EETs) relax vascular smooth muscle by activating potassium channels and causing membrane hyperpolarization. Recent evidence suggests that EETs act via a membrane binding site or receptor. To further characterize this binding site or receptor, we synthesized 20-iodo-14,15-...

journal_title：Biochemistry

authors： Chen Y,Falck JR,Manthati VL,Jat JL,Campbell WB

更新日期：2011-05-10 00:00:00

abstract：:Coenzyme A (CoASH) is the major low-molecular weight thiol in Staphylococcus aureus and a number of other bacteria; the crystal structure of the S. aureus coenzyme A-disulfide reductase (CoADR), which maintains the reduced intracellular state of CoASH, has recently been reported [Mallett, T.C., Wallen, J.R., Karplus, ...

journal_title：Biochemistry

authors： Nicely NI,Parsonage D,Paige C,Newton GL,Fahey RC,Leonardi R,Jackowski S,Mallett TC,Claiborne A

更新日期：2007-03-20 00:00:00

abstract：:Reticulocyte lysate contains a chaperone system that assembles glucocorticoid receptor (GR).hsp90 heterocomplexes. Using purified proteins, we have prepared a five-protein heterocomplex assembly system consisting of two proteins essential for heterocomplex assembly-hsp90 and hsp70-and three proteins that act as co-cha...

journal_title：Biochemistry

authors： Kanelakis KC,Murphy PJ,Galigniana MD,Morishima Y,Takayama S,Reed JC,Toft DO,Pratt WB

更新日期：2000-11-21 00:00:00

abstract：:Human immunodeficiency virus type 1 (HIV-1) Vpu is an integral membrane protein that belongs to the viroporin family. Viroporins interact with cell membranes, triggering membrane permeabilization and promoting release of viral particles. In vitro electrophysiological methods have revealed changes in membrane ion curre...

journal_title：Biochemistry

authors： Herrero L,Monroy N,González ME

更新日期：2013-01-08 00:00:00

abstract：:Putative domain--domain interactions of the monomeric bifunctional enzyme indoleglycerol phosphate synthase:phosphoribosyl anthranilate isomerase from Escherichia coli were probed by separating the domains on the gene level and expressing them as monofunctional proteins. The engineered monofunctional enzymes were foun...

journal_title：Biochemistry

authors： Eberhard M,Tsai-Pflugfelder M,Bolewska K,Hommel U,Kirschner K

更新日期：1995-04-25 00:00:00

abstract：:During cellular uptake of fluorescently labeled cell-penetrating peptides (CPPs), intense fluorescent signals are commonly observed in the nucleus of the cell, suggesting intracellular CPP relocation and potential binding to the genome of the host. We therefore investigated the interaction of the CPP HIV-1 Tat(47-57) ...

journal_title：Biochemistry

authors： Ziegler A,Seelig J

更新日期：2007-07-10 00:00:00

abstract：:Tropomyosin is postulated to confer stability to actin filaments in nonmuscle cells. We have found that a nonmuscle tropomyosin isolated from the intestinal epithelium can directly stabilize actin filaments by slowing depolymerization from the pointed, or slow-growing, filament end. Kinetics of elongation and depolyme...

journal_title：Biochemistry

authors： Broschat KO,Weber A,Burgess DR

更新日期：1989-10-17 00:00:00

abstract：:The cation complexation equilibria between ionophore A23187 and several alkaline earth and first transition series divalent cations have been investigated. Absorption and fluorescence spectroscopy were used to monitor the reactions which were studied in solutions of 80% methanol/water, at 25 degrees C, and under condi...

journal_title：Biochemistry

authors： Chapman CJ,Puri AK,Taylor RW,Pfeiffer DR

更新日期：1987-08-11 00:00:00

abstract：:The simplest carbapenem antibiotic, (5R)-carbapen-2-em-3-carboxylic acid, is biosynthesized from primary metabolites in Pectobacterium carotorova by the action of three enzymes, carboxymethylproline synthase (hereafter named CarB), carbapenam synthetase, and carbapenem synthase. CarB, a member of the crotonase superfa...

journal_title：Biochemistry

authors： Gerratana B,Arnett SO,Stapon A,Townsend CA

更新日期：2004-12-21 00:00:00

abstract：:We systematically and quantitatively investigated the structure and thermodynamics of G-quadruplexes of RNAs and corresponding DNAs of the same sequences under molecular crowding conditions that mimic the high osmotic stress induced by the numerous molecules inside of living cells. Structural analyses demonstrated tha...

journal_title：Biochemistry

authors： Zhang DH,Fujimoto T,Saxena S,Yu HQ,Miyoshi D,Sugimoto N

更新日期：2010-06-01 00:00:00

abstract：:RosB catalyzes the formation of 8-aminoriboflavin 5'-phosphate (AFP), the key intermediate in roseoflavin biosynthesis, from the metabolic precursors riboflavin 5'-phosphate (RP, also known as FMN) and glutamate. The conversion of the aromatic methyl group at position 8 in RP into the aromatic amine in AFP occurs via ...

journal_title：Biochemistry

authors： Kapoor I,Nair SK

更新日期：2018-09-11 00:00:00

abstract：:HpaI and BphI are two pyruvate class II aldolases found in aromatic meta-cleavage degradation pathways that catalyze similar reactions but are not related in sequence. Steady-state kinetic analysis of the aldol addition reactions and product inhibition assays showed that HpaI exhibits a rapid equilibrium random order ...

journal_title：Biochemistry

authors： Wang W,Baker P,Seah SY

更新日期：2010-05-04 00:00:00

abstract：:The phosphatidylcholine-preferring phospholipase C from Bacillus cereus (PLCBc) is a 28.5 kDa enzyme with three zinc ions in its active site. Although much is known about the roles that various PLCBc active site amino acids play in binding and catalysis, there is little information about the rate-determining step of t...

journal_title：Biochemistry

authors： Martin SF,Hergenrother PJ

更新日期：1999-04-06 00:00:00

abstract：:In the globin family, similarities in the folding mechanism have been found among different mammalian apomyoglobins (apoMb). The best-characterized intermediate of sperm whale apoMb, called I(AGH), is mainly stabilized by nativelike contacts among the A, G, and H helices involving a cluster of hydrophobic residues tha...

journal_title：Biochemistry

authors： Musto R,Bigotti MG,Travaglini-Allocatelli C,Brunori M,Cutruzzolà F

更新日期：2004-01-13 00:00:00

abstract：:Internal fatty acylation of proteins is a recognized means of modifying biological behavior. Escherichia coli hemolysin A (HlyA), a toxic protein, is transcribed as a nontoxic protein and made toxic by internal acylation of two lysine residue epsilon-amino groups; HlyC catalyzes the acyl transfer from acyl-acyl carrie...

journal_title：Biochemistry

authors： Trent MS,Worsham LM,Ernst-Fonberg ML

更新日期：1999-07-06 00:00:00

abstract：:Over 20 years ago, it was reported that liver cytosol contains at least two distinct proteins that transfer phosphatidylinositol in vitro, phosphatidylinositol transfer protein (PITP) and a pH 5.1 supernatant fraction containing sterol carrier protein-2 (SCP-2). In contrast to PITP, there has been minimal progress on ...

journal_title：Biochemistry

authors： Schroeder F,Zhou M,Swaggerty CL,Atshaves BP,Petrescu AD,Storey SM,Martin GG,Huang H,Helmkamp GM,Ball JM

更新日期：2003-03-25 00:00:00

abstract：:Ferredoxin I from Desulfovibrio africanus (Da FdI) is a small acidic [4Fe-4S] cluster protein that exchanges electrons with pyruvate-ferredoxin oxidoreductase (PFOR), a key enzyme in the energy metabolism of anaerobes. The thermodynamic properties and the electron transfer between PFOR and either native or mutated FdI...

journal_title：Biochemistry

authors： Pieulle L,Nouailler M,Morelli X,Cavazza C,Gallice P,Blanchet S,Bianco P,Guerlesquin F,Hatchikian EC

更新日期：2004-12-14 00:00:00

abstract：:Escherichia coli 16S RNA from 30S ribosomal subunits was isolated, oxidized at the 3' end, and labeled with the thiosemicarbazide derivatives of fluorescein or eosin. Labeled 16S RNA was reconstituted into 30S subunits. They were almost fully active compared to 30S subunits reconstituted from unlabeled 16S RNA by usin...

journal_title：Biochemistry

authors： Robbins D,Odom OW Jr,Lynch J,Kramer G,Hardesty B,Liou R,Ofengand J

更新日期：1981-09-01 00:00:00