Abstract:
:Mitomycins make up a group of antitumor natural products that are biosynthesized from aminohydroxybenzoic acid (AHBA) and N-acetylglucosamine (GlcNAc). While the biosynthetic gene cluster was reported two decades ago, the mechanism by which the two building blocks, AHBA and GlcNAc, are coupled during biosynthesis remained uncharacterized. Here we report evidence that AHBA is first loaded onto an MmcB acyl carrier protein (ACP) by a MitE acyl ACP synthetase, followed by a transfer of GlcNAc from UDP-GlcNAc by MitB. The results suggest that the early steps of mitomycin biosynthesis proceed via intermediates linked to MmcB.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Nguyen HP,Yokoyama Kdoi
10.1021/acs.biochem.9b00379subject
Has Abstractpub_date
2019-06-25 00:00:00pages
2804-2808issue
25eissn
0006-2960issn
1520-4995journal_volume
58pub_type
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