当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > HlyC, the internal protein acyltransferase that activates hemolysin toxin: the role of conserved tyrosine and arginine residues in enzymatic activity as probed by chemical modification and site-directed mutagenesis.

HlyC, the internal protein acyltransferase that activates hemolysin toxin: the role of conserved tyrosine and arginine residues in enzymatic activity as probed by chemical modification and site-directed mutagenesis.

Abstract：

:Internal fatty acylation of proteins is a recognized means of modifying biological behavior. Escherichia coli hemolysin A (HlyA), a toxic protein, is transcribed as a nontoxic protein and made toxic by internal acylation of two lysine residue epsilon-amino groups; HlyC catalyzes the acyl transfer from acyl-acyl carrier protein (ACP), the obligate acyl donor. Conserved residues among the respective homologous C proteins that activate 13 different RTX (repeats in toxin) toxins of which HlyA is the prototype likely include some residues that are important in catalysis. Possible roles of two conserved tyrosines and two conserved arginines were investigated by noting the effects of chemical modifiers and site-directed mutagenesis. TNM modification of HlyC at pH 8.0 led to extensive inhibition that was prevented by the presence of the substrate myristoyl-ACP but not by the product, ACPSH. NAI had no effect. Y70G and Y150G greatly diminished enzyme activity, whereas mutations Y70F and Y150F exhibited wild-type activity. Modification of arginine residues with PG markedly lowered acyltransferase activity with moderate protection by both myristoyl-ACP and ACPSH. Under optimum conditions, four separate mutations of the two conserved arginine residues (R24A, R24K, R87A, and R87K) had little effect on acyltransferase activity.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Trent MS,Worsham LM,Ernst-Fonberg ML

doi

10.1021/bi990138y

subject

Has Abstract

pub_date

1999-07-06 00:00:00

pages

8831-8

issue

27

eissn

0006-2960

issn

1520-4995

pii

bi990138y

journal_volume

38

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Formation of a bis(histidyl) heme iron complex in manganese peroxidase at high pH and restoration of the native enzyme structure by calcium.

    abstract：:Manganese peroxidase (MnP) from Phanerochaete chrysosporium undergoes a pH-dependent conformational change evidenced by changes in the electronic absorption spectrum. This high- to low-spin alkaline transition occurs at approximately 2 pH units lower in an F190I mutant MnP when compared to the wild-type enzyme. Herein...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi000679j

    authors： Youngs HL,Moënne-Loccoz P,Loehr TM,Gold MH

    更新日期：2000-08-15 00:00:00

  • Molecular dynamics simulations of the TrkH membrane protein.

    abstract：:TrkH is a transmembrane protein that mediates uptake of K(+) through the cell membrane. Despite the recent determination of its crystallographic structure, the nature of the permeation mechanism is still unknown, that is, whether K(+) ions move across TrkH by active transport or passive diffusion. Here, molecular dyna...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi201586n

    authors： Domene C,Furini S

    更新日期：2012-02-28 00:00:00

  • Human liver alanine aminopeptidase. Inhibition by amino acids.

    abstract：:Human liver alanine aminopeptidase is inhibited by L-amino acids having hydrophobic side chains such as Phe, Tyr, Trp, Met, and Leu. Blocking of the amino group or the carboxyl group greatly reduces the inhibitory capacity of the amino acid. Kinetic studies demonstrate that inhibition of hydrolysis of the substrate L-...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00685a027

    authors： Garner CW,Behal FJ

    更新日期：1975-07-15 00:00:00

  • Importance of the region around glycine-338 for the activity of enzyme I of the Escherichia coli phosphoenolpyruvate:sugar phosphotransferase system.

    abstract：:The gene encoding enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system from an Escherichia coli enzyme I mutant was cloned and sequenced. The mutation was shown to be a guanine to adenine transition resulting in an altered protein in which glycine-338 was replaced by aspartic acid. The enzyme I structur...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi952052k

    authors： Seok YJ,Lee BR,Gazdar C,Svenson I,Yadla N,Peterkofsky A

    更新日期：1996-01-09 00:00:00

  • Hydrophobic mismatch and the incorporation of peptides into lipid bilayers: a possible mechanism for retention in the Golgi.

    abstract：:Preferential interaction of trans-membrane alpha-helices whose hydrophobic length matches the hydrophobic thickness of the lipid bilayer could be a mechanism of retention in the Golgi apparatus. We have used fluorescence methods to study the interaction of peptides Ac-K2-G-Lm-W-Ln-K2-A-amide (Pm+n) with bilayers of ph...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi972441+

    authors： Webb RJ,East JM,Sharma RP,Lee AG

    更新日期：1998-01-13 00:00:00

  • Solution structures of spinach acyl carrier protein with decanoate and stearate.

    abstract：:Acyl carrier protein (ACP) is a cofactor in a variety of biosynthetic pathways, including fatty acid metabolism. Thus, it is of interest to determine structures of physiologically relevant ACP-fatty acid complexes. We report here the NMR solution structures of spinach ACP with decanoate (10:0-ACP) and stearate (18:0-A...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi052062d

    authors： Zornetzer GA,Fox BG,Markley JL

    更新日期：2006-04-25 00:00:00

  • Circular dichroism studies on the interaction of tryptophan synthase with pyridoxal 5'-phosphate.

    abstract：:The interaction between the beta 2 subunit of tryptophan synthase and the coenzyme pyridoxal 5'-phosphate (PLP) is characterized by induced circular dichroism (CD) in the near-UV (260-285 nm) and in the visible region (320-480 nm, extrinsic Cotton effect). Because of its high mean residue ellipticity ([theta] = 56 deg...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00525a017

    authors： Balk H,Merkl I,Bartholmes P

    更新日期：1981-10-27 00:00:00

  • Oligomeric state and mode of self-association of Thermotoga maritima ribosomal stalk protein L12 in solution.

    abstract：:The "stalk" of the prokaryotic 50S ribosomal subunit is comprised of four copies of the protein L7/L12. In Escherichia coli, L7/L12 is a dimeric protein at micromolar concentrations, which is able to undergo rapid subunit exchange. A recent structural study indicated a tetrameric arrangement of the L12 proteins isolat...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi048015n

    authors： Moens PD,Wahl MC,Jameson DM

    更新日期：2005-03-08 00:00:00

  • Solution structure of the first intracellular loop of prostacyclin receptor and implication of its interaction with the C-terminal segment of G alpha s protein.

    abstract：:The amino acids (residues 39-51) responsible for the interaction between the first intracellular loop (iLP1) of the human prostacyclin receptor (IP) and G alpha s protein have been identified [Zhang, L., Huang, G., Wu, J., and Ruan, K. H. (2005) Biochemistry 44, 11389-11401]. To further characterize the structural/fun...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0515669

    authors： Zhang L,Wu J,Ruan KH

    更新日期：2006-02-14 00:00:00

  • The reactivity of heme in biological systems: autocatalytic formation of both tyrosine-heme and tryptophan-heme covalent links in a single protein architecture.

    abstract：:We have previously shown that introduction of an engineered Met160 residue in ascorbate peroxidase (S160M variant) leads to the formation of a covalent link between Met160 and the heme vinyl group [Metcalfe, C. L., et al. (2004) J. Am. Chem. Soc. 126, 16242-16248]. In this work, we have used electronic spectroscopy, H...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi7015316

    authors： Pipirou Z,Bottrill AR,Svistunenko DA,Efimov I,Basran J,Mistry SC,Cooper CE,Raven EL

    更新日期：2007-11-20 00:00:00

  • Homo- and heteronuclear two-dimensional NMR studies of the globular domain of histone H1: full assignment, tertiary structure, and comparison with the globular domain of histone H5.

    abstract：:The globular domain of chicken histone H1 (GH1) has been studied by 1H homonuclear and 1H-15N heteronuclear 2D NMR spectroscopy. After the full assignment of the proton and 15N resonances, the tertiary structure of GH1 was determined by an iterative procedure using distance geometry and restrained simulated annealing....

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00203a004

    authors： Cerf C,Lippens G,Ramakrishnan V,Muyldermans S,Segers A,Wyns L,Wodak SJ,Hallenga K

    更新日期：1994-09-20 00:00:00

  • Structure of botulinum neurotoxin type D light chain at 1.65 A resolution: repercussions for VAMP-2 substrate specificity.

    abstract：:The seven serotypes (A-G) of botulinum neurotoxins (BoNTs) function through their proteolytic cleavage of one of three proteins (SNAP-25, Syntaxin, and VAMP) that form the SNARE complex required for synaptic vesicle fusion. The different BoNTs have very specific protease recognition requirements, between 15 and 50 ami...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi052518r

    authors： Arndt JW,Chai Q,Christian T,Stevens RC

    更新日期：2006-03-14 00:00:00

  • Identification of basic amino acid residues important for citrate binding by the periplasmic receptor domain of the sensor kinase CitA.

    abstract：:The sensor kinase CitA and the response regulator CitB of Klebsiella pneumoniae form the paradigm of a subfamily of bacterial two-component regulatory systems that are capable of sensing tri- or dicarboxylates in the environment and then induce transporters for the uptake of these compounds. We recently showed that th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0340595

    authors： Gerharz T,Reinelt S,Kaspar S,Scapozza L,Bott M

    更新日期：2003-05-20 00:00:00

  • Poliovirus RNA-dependent RNA polymerase (3Dpol): kinetic, thermodynamic, and structural analysis of ribonucleotide selection.

    abstract：:We have performed a kinetic and thermodynamic analysis of 3D(pol) derivatives containing substitutions in the ribose-binding pocket with ATP analogues containing correct and incorrect sugar configurations. We find that Asp-238, a residue in structural motif A that is conserved in all RNA-dependent RNA polymerases, is ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi035429s

    authors： Gohara DW,Arnold JJ,Cameron CE

    更新日期：2004-05-11 00:00:00

  • Identification of tyrosine residues that are susceptible to lactoperoxidase-catalyzed iodination on the surface of Escherichia coli 50s ribosomal subunits or 70s ribosomes.

    abstract：:Further to our studies on the Escherichia coli 30S ribosomal subunit, the detailed surface topography of both 50S subunits and 70S ribosomes has been investigated by using iodination catalyzed by immobilized lactoperoxidase as the surface probe. In the 50S subunit, only proteins L2, L5, L10, and L11 were iodinated to ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00282a019

    authors： Maly P,Wower J,Zobawa M,Brimacombe R

    更新日期：1983-06-21 00:00:00

  • Structural analysis of O2'-methyl-5-carbamoylmethyluridine, a newly discovered constituent of yeast transfer RNA.

    abstract：:A compound tentatively identified as O2-methyl-5-carboxymethyluridine (cm5Um) was recently isolated in this laboratory from bulk yeast transfer RNA (Gray, M. W. (1975), Can, J. Biochem. 53, 735-746). Alkaline hydrolysis of yeast tRNA releases this nucleoside as part of an alkali-stable dinucleotide, cm5Um-Ap, from whi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00659a017

    authors： Gray MW

    更新日期：1976-07-13 00:00:00

  • pH-dependent properties of cobalt(II) carboxypeptidase A-inhibitor complexes.

    abstract：:1H NMR spectroscopy of the isotropically shifted signals in cobalt carboxypeptidase, CoCPD, permits a direct and selective detection of protons belonging to the residues liganded to the metal. The chemical shift of these protons in the free enzyme and enzyme-inhibitor complexes with changing pH monitors the state of i...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00130a015

    authors： Auld DS,Bertini I,Donaire A,Messori L,Moratal JM

    更新日期：1992-04-21 00:00:00

  • Pyrrolopyridine derivatives from pyridoxal 5'-sulfate.

    abstract：:A fluorescent derivative of lysine-258 isolated from the active site of aspartate aminotransferase modified by treatment of the apoenzyme with pyridoxal 5'-sulfate has been characterized as a substituted 2H-pyrrolo[3,4-c]pyridine. Similar pyrrolopyridines are produced in up to 20% yield by reaction of pyridoxal sulfat...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00264a016

    authors： Scott RD,Ueno H,Metzler DE

    更新日期：1982-10-12 00:00:00

  • Escherichia coli cystathionine gamma-synthase does not obey ping-pong kinetics. Novel continuous assays for the elimination and substitution reactions.

    abstract：:Cystathionine gamma-synthase (CGS) is a pyridoxal phosphate-dependent enzyme that catalyzes a gamma-replacement reaction, in which the succinyl group of an O-succinyl-L-homoserine (L-OSHS) is displaced by the thiol of L-cysteine to form L-cystathionine, in the first step of the bacterial transsulfuration pathway. The ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi035107o

    authors： Aitken SM,Kim DH,Kirsch JF

    更新日期：2003-09-30 00:00:00

  • GrpE accelerates nucleotide exchange of the molecular chaperone DnaK with an associative displacement mechanism.

    abstract：:The ATP hydrolysis and protein-binding and release cycle of the molecular chaperone DnaK is regulated by the accessory proteins GrpE and DnaJ. Here we describe a study of the formation of complexes between the molecular chaperone DnaK, its nucleotide exchange factor GrpE, and the fluorescent ADP analog N8-[4-[(N'-meth...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi962835l

    authors： Packschies L,Theyssen H,Buchberger A,Bukau B,Goody RS,Reinstein J

    更新日期：1997-03-25 00:00:00

  • Determination of Fe-ligand bond lengths and the Fe-N-O bond angles in soybean ferrous and ferric nitrosylleghemoglobin a using multiple-scattering XAFS analyses.

    abstract：:The NO adducts of leghemoglobin (Lb) are implicated in biological processes, but only the adduct with ferrous Lb (Lb(II)NO) has been characterized previously. We report the first characterization of ferric nitrosylleghemoglobin (Lb(III)NO) and XAS experiments performed on frozen aqueous solutions of Lb(II)NO and Lb(II...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi990730n

    authors： Rich AM,Ellis PJ,Tennant L,Wright PE,Armstrong RS,Lay PA

    更新日期：1999-12-14 00:00:00

  • Folding domains as functional tools in allosteric systems: a heme-dependent domain in hemoglobin beta subunits.

    abstract：:We have investigated the denaturation by guanidine hydrochloride (Gdn X HCl), temperature, and pH of hemoglobin beta subunits and of the peptides beta (1-146), beta (56-146), and beta (1-55). The last peptide was insensitive to all of the three agents. In the other polypeptides denaturation by Gdn X HCl and temperatur...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00267a024

    authors： Franchi D,Fronticelli C,Bucci E

    更新日期：1982-11-23 00:00:00

  • Probing the nucleotide binding sites of axonemal dynein with the fluorescent nucleotide analogue 2'(3')-O-(-N-Methylanthraniloyl)-adenosine 5'-triphosphate.

    abstract：:MantATP [2'(3')-O-(-N-methylanthraniloyl)-adenosine 5'-triphosphate] was employed as a fluorescence probe of the nucleotide-binding sites of dynein from sea urchin sperm flagella. MantATP binds specifically with enhanced fluorescence (approximately 2.2-fold), homogeneous lifetime (8.4 ns), and high anisotropy (r appro...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9730184

    authors： Mocz G,Helms MK,Jameson DM,Gibbons IR

    更新日期：1998-07-07 00:00:00

  • Absolute action spectrum of E-FADH2 and E-FADH2-MTHF forms of Escherichia coli DNA photolyase.

    abstract：:Escherichia coli DNA photolyase mediates photorepair of pyrimidine dimers occurring in UV-damaged DNA. The enzyme contains two chromophores, 1,5-dihydroflavin adenine dinucleotide (FADH2) and 5,10-methenyltetrahydrofolylpolyglutamate (MTHF). To define the roles of the two chromophores in the photochemical reaction(s) ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00485a021

    authors： Payne G,Sancar A

    更新日期：1990-08-21 00:00:00

  • Neurofibrillar tangle surrogates: histone H1 binding to patterned phosphotyrosine peptide nanotubes.

    abstract：:Living cells contain a range of densely phosphorylated surfaces, including phospholipid membranes, ribonucleoproteins, and nucleic acid polymers. Hyperphosphorylated surfaces also accumulate in neurodegenerative diseases as neurofibrillar tangles. We have synthesized and structurally characterized a precisely patterne...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi500599a

    authors： Li S,Sidorov AN,Mehta AK,Bisignano AJ,Das D,Childers WS,Schuler E,Jiang Z,Orlando TM,Berland K,Lynn DG

    更新日期：2014-07-08 00:00:00

  • Acetaldehyde--enkephalins: structure proof and some conformational deductions from one- and two-dimensional proton nuclear magnetic resonance spectra.

    abstract：:The structure of the adduct formed by reaction of acetaldehyde and Met5-enkephalin has been determined by analysis of 400-MHz proton spectra: two-dimensional J spectroscopy was used to resolve and measure virtually all the overlapping resonances, and decoupling difference spectroscopy was used to assign the resonances...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00516a033

    authors： Gidley MJ,Hall LD,Sanders JK,Summers MC

    更新日期：1981-06-23 00:00:00

  • Laminins and other strange proteins.

    abstract：:Laminins are large multidomain proteins of the extracellular matrix (ECM) with important functions in the development and maintenance of cellular organization and supramolecular structure, in particular in basement membranes. Each molecule is composed of three polypeptide chains, A (300-400 kDa) and B1 and B2 (180-200...

    journal_title：Biochemistry

    pub_type： 杂志文章,评审

    doi：10.1021/bi00159a001

    authors： Engel J

    更新日期：1992-11-10 00:00:00

  • Thermodynamics of nucleotide and inhibitor binding to wild-type and ispinesib-resistant forms of human kinesin spindle protein.

    abstract：:Current antimitotic cancer chemotherapy based on vinca alkaloids and taxanes target tubulin, a protein required not only for mitotic spindle formation but also for the overall structural integrity of terminally differentiated cells. Among many innovations targeting specific mitotic events, inhibition of motor enzymes ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi900946r

    authors： Sheth PR,Basso A,Duca JS,Lesburg CA,Ogas P,Gray K,Nale L,Mannarino AF,Prongay AJ,Le HV

    更新日期：2009-11-24 00:00:00

  • Estimation of the distance change between cysteine-457 and the nucleotide binding site when sodium pump changes conformation from E1 to E2 by fluorescence energy transfer measurements.

    abstract：:The first indication of the size of a conformational change implicated in ion transport by sodium pump has been obtained by measuring the change in efficiency of fluorescence energy transfer between two specific locations on the alpha-subunit. The donor (5'-(iodoacetamido)fluorescein) attaches covalently to cysteine-4...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi960407+

    authors： Lin SH,Faller LD

    更新日期：1996-06-25 00:00:00

  • Effect of alpha-methylation on inactivation of monoamine oxidase by N-cyclopropylbenzylamine.

    abstract：:Monoamine oxidase (MAO) was shown previously [Silverman, R. B., & Hoffman, S. J. (1980) J. Am. Chem. Soc. 102, 7126-7128] to catalyze the oxidation of N-cyclopropylbenzylamine (N-CBA) at two sites on the molecule. Oxidation at the benzyl methylene gave benzaldehyde and cyclopropylamine; oxidation of the cyclopropyl gr...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00317a019

    authors： Silverman RB

    更新日期：1984-10-23 00:00:00