Molybdate-stabilized glucocorticoid receptor: evidence for a receptor heteromer.

abstract：:Apolipophorin III (apoLp-III) from the greater wax moth Galleria mellonella is an exchangeable insect apolipoprotein that consists of five amphipathic alpha-helices, sharing high sequence identity with apoLp-III from the sphinx moth Manduca sexta whose structure is available. To define the minimal requirement for apoL...

journal_title：Biochemistry

authors： Dettloff M,Weers PM,Niere M,Kay CM,Ryan RO,Wiesner A

更新日期：2001-03-13 00:00:00

abstract：:Decorin-binding protein A (DBPA) is an important lipoprotein from the bacterium Borrelia burgdorferi, the causative agent of Lyme disease. The absence of DBPA drastically reduces the pathogenic potential of the bacterium, and biochemical evidence indicates DBPA's interactions with the glycosaminoglycan (GAG) portion o...

journal_title：Biochemistry

authors： Wang X

更新日期：2012-10-23 00:00:00

abstract：:Large conductance Ca(2+)-activated K(+) channels (BK(Ca)) contain an intracellular binding site for bovine pancreatic trypsin inhibitor (BPTI), a well-known inhibitor of various serine proteinase (SerP) enzymes. To investigate the structural basis of this interaction, we examined the activity of 11 BPTI mutants using ...

journal_title：Biochemistry

authors： Favre I,Moss GW,Goldenberg DP,Otlewski J,Moczydlowski E

更新日期：2000-02-29 00:00:00

abstract：:This paper reports on a solution NMR characterization of the sequence selectivity and metal ion specificity in chromomycin-DNA oligomer complexes in the presence of divalent cations. The sequence selectivity studies have focused on chromomycin complexes with the self-complementary d(A1-A2-G3-G4-C5-C6-T7-T8) duplex con...

journal_title：Biochemistry

authors： Gao XL,Patel DJ

更新日期：1990-12-11 00:00:00

abstract：:Several enzymatic and chemical reagents were used to probe the secondary structure of Saccharomyces cerevisiae nuclear RNase P RNA in the presence and absence of its protein components. Double-stranded regions were detected with RNase V1 and single-stranded regions with RNase ONE (Escherichia coli RNase I). Nucleotide...

journal_title：Biochemistry

authors： Tranguch AJ,Kindelberger DW,Rohlman CE,Lee JY,Engelke DR

更新日期：1994-02-22 00:00:00

abstract：:The mechanism of chromophore biosynthesis in green fluorescent protein (GFP) is triggered by a spontaneous main chain cyclization reaction of residues 65-67. Here, we demonstrate that the initially colorless Y66L variant, designed to trap chromophore precursor states, is oxidatively modified to generate yellow chromop...

journal_title：Biochemistry

authors： Rosenow MA,Patel HN,Wachter RM

更新日期：2005-06-14 00:00:00

abstract：:The electron spin resonance (ESR) spectra from spin-labeled phospholipids in recombinants of myelin proteolipid apoprotein with dimyristoylphosphatidylcholine have been simulated with the exchanged-coupled Bloch equations to obtain values for both the fraction of motionally restricted lipids and the exchange rate betw...

journal_title：Biochemistry

authors： Horváth LI,Brophy PJ,Marsh D

更新日期：1988-01-12 00:00:00

abstract：:Specific interactions of human melanocortin-4 receptor (hMC4R) with its nonpeptide and peptide agonists were studied using alanine-scanning mutagenesis. The binding affinities and potencies of two synthetic, small-molecule agonists (THIQ, MB243) were strongly affected by substitutions in transmembrane alpha-helices (T...

journal_title：Biochemistry

authors： Pogozheva ID,Chai BX,Lomize AL,Fong TM,Weinberg DH,Nargund RP,Mulholland MW,Gantz I,Mosberg HI

更新日期：2005-08-30 00:00:00

abstract：:Pulmonary surfactant forms a monolayer of lipids and proteins at the alveolar air/liquid interface. Although cholesterol is a natural component of surfactant, its function in surface dynamics is unclear. To further elucidate the role of cholesterol in surfactant, we used a captive bubble surfactometer (CBS) to measure...

journal_title：Biochemistry

authors： Diemel RV,Snel MM,Van Golde LM,Putz G,Haagsman HP,Batenburg JJ

更新日期：2002-12-17 00:00:00

abstract：:A molecular model has been built of the equine infectious anemia virus (EIAV) proteinase on the basis of the crystal structures of the related Rous sarcoma virus (RSV) and human immunodeficiency virus (HIV) proteinases. The 104 residue long EIAV proteinase has 30 identical and 11 similar amino acids compared to those ...

journal_title：Biochemistry

authors： Weber IT,Tözsér J,Wu J,Friedman D,Oroszlan S

更新日期：1993-04-06 00:00:00

abstract：:The human translin protein binds a variety of sequences (chromosomal breakpoint consensus sequences, their sequence variants, as well as nonbreakpoint sequences such as simple AT and GC repeats) at nanomolar protein concentration when short single strands ( approximately 20-30mers) are used as DNA targets. The protein...

journal_title：Biochemistry

authors： Sengupta K,Rao BJ

更新日期：2002-12-24 00:00:00

abstract：:ATP, its nonhydrolyzable analogue, AMP-PNP, and albumin were found to promote the dissociation of rhodopsin kinase from rod outer segments (ROS) containing photoactivated-rhodopsin (Rho*). These features were embodied in a protocol for the recovery of rhodopsin kinase from incubations containing ROS which had been sub...

journal_title：Biochemistry

authors： Dean KR,Akhtar M

更新日期：1996-05-14 00:00:00

abstract：:The solution structure of the 63-residue heregulin-alpha (HRG-alpha) epidermal growth factor (EGF)-like domain, corresponding to residues 177-239 of HRG-alpha, has been determined to high resolution using data from two-dimensional and three-dimensional homo- and heteronuclear NMR spectroscopy. The structure is based o...

journal_title：Biochemistry

authors： Jacobsen NE,Abadi N,Sliwkowski MX,Reilly D,Skelton NJ,Fairbrother WJ

更新日期：1996-03-19 00:00:00

abstract：:The correlation of the UVRR nuW3 mode with the tryptophan chi(2,1) dihedral angle [Maruyama and Takeuchi (1995) J. Raman Spectrosc. 26, 319; Miura et al. (1989) J. Raman Spectrosc. 20, 667; Takeuchi (2003) Biopolymers 72, 305] has been extended to a full, 360 degrees rotation. The 3-fold periodicity of the relationshi...

journal_title：Biochemistry

authors： Juszczak LJ,Desamero RZ

更新日期：2009-03-31 00:00:00

abstract：:Deletion of two of the three homologous lipoyl domains that form the N-terminal half of each dihydrolipoamide acetyltransferase (E2p) polypeptide chain of the Escherichia coli pyruvate dehydrogenase complex can be achieved by in vitro deletion in the structural gene aceF. A site-directed mutagenesis of this shortened ...

journal_title：Biochemistry

authors： Texter FL,Radford SE,Laue ED,Perham RN,Miles JS,Guest JR

更新日期：1988-01-12 00:00:00

abstract：:The formation of amyloid fibril is associated with major human diseases, including Alzheimer's disease, prion diseases, and type 2 diabetes. Methods for efficient inhibition of amyloid fibril formation are therefore highly clinically important. A principal approach for the inhibition of amyloid formation is based on t...

journal_title：Biochemistry

authors： Porat Y,Mazor Y,Efrat S,Gazit E

更新日期：2004-11-16 00:00:00

abstract：:Ribulose-1,5-bisphosphate (RuBP) carboxylase/oxygenase (Rubisco) is a globally significant biocatalyst that facilitates the removal and sequestration of CO2 from the biosphere. Rubisco-catalyzed CO2 reduction thus provides virtually all of the organic carbon utilized by living organisms. Despite catalyzing the rate-li...

journal_title：Biochemistry

authors： Satagopan S,Scott SS,Smith TG,Tabita FR

更新日期：2009-09-29 00:00:00

abstract：:Computer graphics and energy minimization techniques were used to construct a model of DNA containing cis-thymine glycol, an oxidation product of thymine formed in DNA by ionizing radiation. The model simulated an experimental DNA substrate used to study the effects of this lesion on DNA synthesis in vitro. The result...

journal_title：Biochemistry

authors： Clark JM,Pattabiraman N,Jarvis W,Beardsley GP

更新日期：1987-08-25 00:00:00

abstract：:Human melanin-concentrating hormone (hMCH) and many of its analogues are potent but nonspecific ligands for human melanin-concentrating hormone receptors 1 and 2 (hMCH-1R and hMCH-2R). To differentiate between the physiological functions of these receptors, selective antagonists are needed. In this study, analogues of...

journal_title：Biochemistry

authors： Bednarek MA,Hreniuk DL,Tan C,Palyha OC,MacNeil DJ,Van der Ploeg LH,Howard AD,Feighner SD

更新日期：2002-05-21 00:00:00

abstract：:The two flavodoxins (YkuN and YkuP) from Bacillus subtilis have been cloned, overexpressed in Escherichia coli and purified. DNA sequencing, mass spectrometry, and flavin-binding properties showed that both YkuN and YkuP were typical short-chain flavodoxins (158 and 151 amino acids, respectively) and that an error in ...

journal_title：Biochemistry

authors： Lawson RJ,von Wachenfeldt C,Haq I,Perkins J,Munro AW

更新日期：2004-10-05 00:00:00

abstract：:The unfolding and refolding of alpha-helical proteins has been extensively studied, demonstrating formation of intermediate structures which retain the native-like alpha-helix but lack the tertiary structure. Studies on the folding of proteins consisting primarily of beta-sheet are interesting since, unlike the alpha-...

journal_title：Biochemistry

authors： Narhi LO,Philo JS,Li T,Zhang M,Samal B,Arakawa T

更新日期：1996-09-03 00:00:00

abstract：:How various anti-amyloidogenic compounds inhibit the formation of Alzheimer's beta-amyloid fibrils (fAbeta) from amyloid beta-peptide (Abeta) and destabilize fAbeta remains poorly understood. Using spectrophotometry, spectrofluorometry, atomic force microscopy, sodium dodecyl sulfate-polyacrylamide gel electrophoresis...

journal_title：Biochemistry

authors： Hirohata M,Hasegawa K,Tsutsumi-Yasuhara S,Ohhashi Y,Ookoshi T,Ono K,Yamada M,Naiki H

更新日期：2007-02-20 00:00:00

abstract：:The physiological effects of anesthetics have been ascribed to their interaction with hydrophobic sites within functionally relevant CNS proteins. Studies have shown that volatile anesthetics compete for luciferin binding to the hydrophobic substrate binding site within firefly luciferase and inhibit its activity (Fra...

journal_title：Biochemistry

authors： Ho C,Shanmugasundararaj S,Miller KW,Malinowski SA,Cook AC,Slater SJ

更新日期：2008-09-09 00:00:00

abstract：:The solution structure of the actinorhodin acyl carrier protein (act apo-ACP) from the polyketide synthase (PKS) of Streptomyces coelicolor A3(2) has been determined using 1H NMR spectroscopy, representing the first polyketide synthase component for which detailed structural information has been obtained. Twenty-four ...

journal_title：Biochemistry

authors： Crump MP,Crosby J,Dempsey CE,Parkinson JA,Murray M,Hopwood DA,Simpson TJ

更新日期：1997-05-20 00:00:00

abstract：:In an effort to gain greater insight into the molecular mechanism of the electron-transfer reactions of cytochrome b(5), the bovine cytochrome b(5)-horse cytochrome c complex has been investigated by high-resolution multidimensional NMR spectroscopy using (13)C, (15)N-labeled cytochrome b(5) expressed from a synthetic...

journal_title：Biochemistry

authors： Hom K,Ma QF,Wolfe G,Zhang H,Storch EM,Daggett V,Basus VJ,Waskell L

更新日期：2000-11-21 00:00:00

abstract：:The fluorescence of the base analogue 2-aminopurine (2AP) was used to probe bacteriophage T4 DNA polymerase-induced conformational changes in the template strand produced during the nucleotide incorporation and proofreading reactions. 2AP fluorescence in DNA is quenched by 2AP interactions with neighboring bases, but ...

journal_title：Biochemistry

authors： Hariharan C,Reha-Krantz LJ

更新日期：2005-12-06 00:00:00

abstract：:The photocycle of the blue-light photoreceptor protein Photoactive Yellow Protein (PYP) was studied at reduced relative humidity (RH). Photocycle kinetics and spectra were measured in thin films of PYP in which the relative humidity was set at values between 29 and 98% RH with saturated solutions of various salts. We ...

journal_title：Biochemistry

authors： van der Horst MA,van Stokkum IH,Dencher NA,Hellingwerf KJ

更新日期：2005-06-28 00:00:00

abstract：:The potential of the frequently encountered (βα)8-barrel fold to acquire new functions was tested by an approach combining random mutagenesis and selection in vivo. For this purpose, the genes encoding 52 different phosphate-binding (βα)8-barrel proteins were subjected to error-prone PCR and cloned into an expression ...

journal_title：Biochemistry

authors： Rohweder B,Lehmann G,Eichner N,Polen T,Rajendran C,Ruperti F,Linde M,Treiber T,Jung O,Dettmer K,Meister G,Bott M,Gronwald W,Sterner R

更新日期：2019-10-15 00:00:00

abstract：:Studying the evolution of catalytically promiscuous enzymes like those from the N-succinylamino acid racemase/ o-succinylbenzoate synthase (NSAR/OSBS) subfamily can reveal mechanisms by which new functions evolve. Some enzymes in this subfamily have only OSBS activity, while others catalyze OSBS and NSAR reactions. We...

journal_title：Biochemistry

authors： Odokonyero D,McMillan AW,Ramagopal UA,Toro R,Truong DP,Zhu M,Lopez MS,Somiari B,Herman M,Aziz A,Bonanno JB,Hull KG,Burley SK,Romo D,Almo SC,Glasner ME

更新日期：2018-07-03 00:00:00

abstract：:Thirty single cysteine substitution mutants of T4 lysozyme have been prepared and spin-labeled with a sulfhydryl-specific nitroxide reagent in order to systematically investigate the relationship between nitroxide side-chain mobility and protein structure. The perturbation caused by replacement of a native residue wit...

journal_title：Biochemistry

authors： Mchaourab HS,Lietzow MA,Hideg K,Hubbell WL

更新日期：1996-06-18 00:00:00