Extension of the tryptophan chi2,1 dihedral angle-W3 band frequency relationship to a full rotation: correlations and caveats.

abstract：:It is now well established that guanine nucleotides are allosteric effectors of the vinca alkaloid-induced self-association of tubulin. GDP enhances self-association for vinblastine-, vincristine- and vinorelbine-induced spiral assembly relative to GTP by 0.90 +/- 0.17 kcal/mol [Lobert et al. (1996) Biochemistry 35, 6...

journal_title：Biochemistry

authors： Vulevic B,Lobert S,Correia JJ

更新日期：1997-10-21 00:00:00

abstract：:This paper reports on a solution NMR characterization of the sequence selectivity and metal ion specificity in chromomycin-DNA oligomer complexes in the presence of divalent cations. The sequence selectivity studies have focused on chromomycin complexes with the self-complementary d(A1-A2-G3-G4-C5-C6-T7-T8) duplex con...

journal_title：Biochemistry

authors： Gao XL,Patel DJ

更新日期：1990-12-11 00:00:00

abstract：:Two major neutral glyceroglucolipids (A and B) have been isolated from lipid extract of human gastric content by the procedure involving column fractionation on DEAE-Sephadex, silicic acid, and thin-layer chromatography. Both glycolipids contained glucose, glyceryl ethers, and fatty acids. The structures of these glyc...

journal_title：Biochemistry

authors： Slomiany BL,Slomiany A,Glass GB

更新日期：1977-09-06 00:00:00

abstract：:Heme oxygenase (HO) catalyzes the regiospecific cleavage of the porphyrin ring of heme using reducing equivalents and O2 to produce biliverdin, iron, and CO. Because CO has a cytoprotective effect through the p38-MAPK pathway, HO is a potential therapeutic target in cancer. In fact, inhibition of the HO isoform HO-1 r...

journal_title：Biochemistry

authors： Sugishima M,Higashimoto Y,Oishi T,Takahashi H,Sakamoto H,Noguchi M,Fukuyama K

更新日期：2007-02-20 00:00:00

abstract：:The distance between the calcium site (site I) and the zinc site (site II) in alpha-lactalbumin was estimated from Forster energy-transfer measurements between donor Eu(III) [or Tb(III)] at site I and acceptor Co(II) at site II to be 11.5 +/- 1.5 A. Intersite distances were also measured between the bis-ANS [4,4'-bis[...

journal_title：Biochemistry

authors： Musci G,Berliner LJ

更新日期：1986-08-26 00:00:00

abstract：:Glutamate semialdehyde aminomutase is a recognized target for selective herbicides and antibacterial agents because it provides the aminolevulinate from which tetrapyrroles are synthesized in plants and bacteria but not in animals. The reactions of the enzyme with R- and S-enantiomers of a novel compound, diaminopropy...

journal_title：Biochemistry

authors： Contestabile R,Jenn T,Akhtar M,Gani D,John RA

更新日期：2000-03-21 00:00:00

abstract：:A conformational change in the DNA plasmid ColE1 appears to occur upon specific binding of the restriction endonuclease EcoRI. Enzyme association alters the chiral discrimination found in binding metallointercalators to DNA sites. The complexes tris(1,10-phenanthroline)ruthenium(II), Ru(phen)3(2+), tris(4,7-diphenyl-1...

journal_title：Biochemistry

authors： Barton JK,Paranawithana SR

更新日期：1986-04-22 00:00:00

abstract：:We report differences in the interaction of two structurally similar phenanthroline intercalators, ethidium and propidium, with poly(dA).poly(dT) and poly[d(A-T)] as a function of ionic strength based on titration microcalorimetry, fluorescence titration, and hydrostatic pressure measurements. Both ethidium and propid...

journal_title：Biochemistry

authors： Marky LA,Macgregor RB Jr

更新日期：1990-05-22 00:00:00

abstract：:We have studied carbon monoxide (CO) migration and binding in the nonsymbiotic hemoglobins AHb1 and AHb2 of Arabidopsis thaliana using Fourier transform infrared (FTIR) spectroscopy combined with temperature derivative spectroscopy (TDS) at cryogenic temperatures. Both proteins have similar amino acid sequences but di...

journal_title：Biochemistry

authors： Nienhaus K,Dominici P,Astegno A,Abbruzzetti S,Viappiani C,Nienhaus GU

更新日期：2010-09-07 00:00:00

abstract：:D-Xylose isomerase (XI) and triosephosphate isomerase (TIM) catalyze the aldose-ketose isomerization reactions of D-xylose and d-glyceraldehyde 3-phosphate (DGAP), respectively. D-Glyceraldehyde (DGA) is the triose fragment common to the substrates for XI and TIM. The XI-catalyzed isomerization of DGA to give dihydrox...

journal_title：Biochemistry

authors： Toteva MM,Silvaggi NR,Allen KN,Richard JP

更新日期：2011-11-22 00:00:00

abstract：:DNA polymerase delta from calf thymus has been purified to apparent homogeneity by a new procedure which utilizes hydrophobic interaction chromatography with phenyl-Sepharose at an early step to separate most of the calcium-dependent protease activity from DNA polymerase delta and alpha. The purified enzyme migrates a...

journal_title：Biochemistry

authors： Lee MY,Tan CK,Downey KM,So AG

更新日期：1984-04-24 00:00:00

abstract：:The potassium form of d(CGCGAATTCGCG) solved by X-ray diffraction to 1.75 A resolution indicates that monovalent cations penetrate the primary and secondary layers of the "spine of hydration". Both the sodium [Shui, X., McFail-Isom, L., Hu, G. G., and Williams, L. D. (1998) Biochemistry 37, 8341-8355] and the potassiu...

journal_title：Biochemistry

authors： Shui X,Sines CC,McFail-Isom L,VanDerveer D,Williams LD

更新日期：1998-12-01 00:00:00

abstract：:The photoaffinity probes [gamma-32P]2-azidoATP (2-N3ATP) and [alpha-32P]8-azido-ATP (8-N3ATP) were used to investigate the binding of ATP to highly purified 2-5A synthetase. 2-N3ATP and 8-N3ATP are substrates for 2-5A synthetase [Suhadolnik, R.J., Karikó, K., Sobol, R.W., Jr., Li, S.W., Reichenbach, N.L., & Haley, B.E...

journal_title：Biochemistry

authors： Suhadolnik RJ,Li SW,Sobol RW Jr,Haley BE

更新日期：1988-11-29 00:00:00

abstract：:The influenza virus RNA-dependent RNA polymerase protein complex contains an associated RNA endonuclease activity, which cleaves host mRNA precursors in the cell nucleus at defined positions 9-15 nucleotides downstream of the cap structure. This reaction provides capped oligoribonucleotides, which function as primers ...

journal_title：Biochemistry

authors： Doan L,Handa B,Roberts NA,Klumpp K

更新日期：1999-04-27 00:00:00

abstract：:Earlier work indicates that the major DNA repair phosphodiesterase (PDE) in yeast cells is the well-characterized Apn1 protein. Apn1 demonstrates both Mg2+-independent PDE activity and Mg2+-independent class II apurinic/apyrimidinic (AP) endonuclease activity and represents greater than 90% of the activity detected in...

journal_title：Biochemistry

authors： Sander M,Ramotar D

更新日期：1997-05-20 00:00:00

abstract：:One way to improve the economic feasibility of biomass conversion is to enhance the catalytic efficiency of cellulases through protein engineering. This requires that high-resolution structures of cellulases be available. Here we present the structure of E2cd, the catalytic domain of the thermophilic endocellulase E2 ...

journal_title：Biochemistry

authors： Spezio M,Wilson DB,Karplus PA

更新日期：1993-09-28 00:00:00

abstract：:Adenylosuccinate lyase, an enzyme catalyzing two reactions in purine biosynthesis (the cleavage of either adenylosuccinate or succinylaminoimidazole carboxamide ribotide), has been implicated in a human disease arising from point mutations in the gene encoding the enzyme. Asn(276) of Bacillus subtilis adenylosuccinate...

journal_title：Biochemistry

authors： Palenchar JB,Colman RF

更新日期：2003-02-25 00:00:00

abstract：:The SH3 and SH2 domains of hematopoietic cell kinase (Hck) play important roles in substrate targeting. To identify new components of Hck signaling pathways, we identified proteins that bind to the SH3 domain of Hck (Scott et al. (2002) J. Biol. Chem. 277, 28238). One such protein was ELMO1, the mammalian orthologue o...

journal_title：Biochemistry

authors： Yokoyama N,deBakker CD,Zappacosta F,Huddleston MJ,Annan RS,Ravichandran KS,Miller WT

更新日期：2005-06-21 00:00:00

abstract：:A study of the voltage induction of transient pores in phospholipid bilayer vesicles is reported. Unilamellar vesicles (dipalmitoylphosphatidylcholine), with a size distribution of 100 +/- 30 nm, were prepared by the method of Enoch & Strittmatter [Enoch, H., & Strittmatter, P. (1979) Proc. Natl. Acad. Sci. U.S.A. 76,...

journal_title：Biochemistry

authors： Teissie J,Tsong TY

更新日期：1981-03-17 00:00:00

abstract：:The molecular mechanism of O2 binding to hemoglobin (Hb) and myoglobin (Mb) is a long-standing issue in the field of bioinorganic and biophysical chemistry. The nature of Fe-O2 bond in oxy Hb and Mb had been extensively investigated by resonance Raman spectroscopy, which assigned the Fe-O2 stretching bands at ∼570 cm-...

journal_title：Biochemistry

authors： Ohta T,Shibata T,Kobayashi Y,Yoda Y,Ogura T,Neya S,Suzuki A,Seto M,Yamamoto Y

更新日期：2018-12-04 00:00:00

abstract：:Pump-1 cDNA has recently been isolated by screening a human tumor cDNA library with a transin (rat stromelysin) probe under low-stringency hybridization conditions. The cDNA codes for a potential protein with significant sequence similarity to the metalloproteinases collagenase and stromelysin, but which lacks the hem...

journal_title：Biochemistry

authors： Quantin B,Murphy G,Breathnach R

更新日期：1989-06-27 00:00:00

abstract：:The human, cytosolic enzyme isocitrate dehydrogenase 1 (IDH1) reversibly converts isocitrate to α-ketoglutarate (αKG). Cancer-associated somatic mutations in IDH1 result in a loss of this normal function but a gain in a new or neomorphic ability to convert αKG to the oncometabolite 2-hydroxyglutarate (2HG). To improve...

journal_title：Biochemistry

authors： Rendina AR,Pietrak B,Smallwood A,Zhao H,Qi H,Quinn C,Adams ND,Concha N,Duraiswami C,Thrall SH,Sweitzer S,Schwartz B

更新日期：2013-07-02 00:00:00

abstract：:Specific interactions of human melanocortin-4 receptor (hMC4R) with its nonpeptide and peptide agonists were studied using alanine-scanning mutagenesis. The binding affinities and potencies of two synthetic, small-molecule agonists (THIQ, MB243) were strongly affected by substitutions in transmembrane alpha-helices (T...

journal_title：Biochemistry

authors： Pogozheva ID,Chai BX,Lomize AL,Fong TM,Weinberg DH,Nargund RP,Mulholland MW,Gantz I,Mosberg HI

更新日期：2005-08-30 00:00:00

abstract：:Acyclovir triphosphate, ganciclovir triphosphate and penciclovir triphosphate inhibited DNA polymerases alpha, delta, and epsilon. Each triphosphate preferentially inhibited pol delta, although ganciclovir triphosphate was the most impressive of the three; the Ki for inhibition of pol delta was 2 microM (competitive w...

journal_title：Biochemistry

authors： Ilsley DD,Lee SH,Miller WH,Kuchta RD

更新日期：1995-02-28 00:00:00

abstract：:Using 125I-labeled phenol-alanine sparsomycin, an analogue of sparsomycin having higher biological activity than the unmodified antibiotic, we studied the requirements and the characteristics of its interaction with the ribosome. The drug does not bind to either isolated ribosomal subunits or reconstituted whole ribos...

journal_title：Biochemistry

authors： Lazaro E,van den Broek LA,San Felix A,Ottenheijm HC,Ballesta JP

更新日期：1991-10-08 00:00:00

abstract：:Thrombospondin-1 (TSP1) binding to calreticulin (CRT) on the cell surface signals focal adhesion disassembly, leading to the intermediate adhesive phenotype, cell migration, anoikis resistance, and collagen stimulation. Residues Lys 24 and 32 in TSP1 and amino acids 24-26 and 32-34 in CRT have been shown through bioch...

journal_title：Biochemistry

authors： Yan Q,Murphy-Ullrich JE,Song Y

更新日期：2011-02-01 00:00:00

abstract：:The reaction between chymotrypsin and N-acetyl-L-phenylalanine p-nitroanilide has been studied at subzero temperatures in fluid aqueous dimethyl sulfoxide solvent. Following initiation of the reaction at temperatures as low as -90 degrees C, a series of four reactions prior to the normal rate-limiting step (acylation)...

journal_title：Biochemistry

authors： Fink AL

更新日期：1976-04-06 00:00:00

abstract：:The vibrations of a water molecule in the water-oxidizing complex (WOC) of photosystem II were detected for the first time using Fourier transform infrared (FTIR) spectroscopy. In a flash-induced FTIR difference spectrum upon the S(1)-to-S(2) transition, a pair of positive and negative bands was observed at 3618 and 3...

journal_title：Biochemistry

authors： Noguchi T,Sugiura M

更新日期：2000-09-12 00:00:00

abstract：:Some key chloroplast enzymes are activated by light via a ferredoxin-thioredoxin reduction system which reduces disulfide bridges in the enzymes. We describe for the first time the structural basis for the redox activation of a chloroplast enzyme, the NADP-dependent malate dehydrogenase (MDH) from Sorghum vulgare whos...

journal_title：Biochemistry

authors： Johansson K,Ramaswamy S,Saarinen M,Lemaire-Chamley M,Issakidis-Bourguet E,Miginiac-Maslow M,Eklund H

更新日期：1999-04-06 00:00:00

abstract：:The signaling apparatus that controls bacterial chemotaxis is composed of a core complex containing chemoreceptors, the histidine autokinase CheA, and the coupling protein CheW. Site-specific spin labeling and pulsed dipolar ESR spectroscopy (PDS) have been applied to investigate the structure of a soluble ternary com...

journal_title：Biochemistry

authors： Bhatnagar J,Borbat PP,Pollard AM,Bilwes AM,Freed JH,Crane BR

更新日期：2010-05-11 00:00:00