Hydration of dA.dT polymers: role of water in the thermodynamics of ethidium and propidium intercalation.

abstract：:Achieving a satisfactory biochemical explanation for the opportunistic underwater adhesion of marine invertebrates such as mussels and barnacles requires a detailed characterization of proteins extracted from holdfast structures produced by these organisms. Mefp-5 is an adhesive protein derived from the foot of the co...

journal_title：Biochemistry

authors： Waite JH,Qin X

更新日期：2001-03-06 00:00:00

abstract：:The assignment of the 1H, 15N, 13CO, and 13C resonances of recombinant human interleukin-4 (IL-4), a protein of 133 residues and molecular mass of 15.4 kDa, is presented based on a series of 11 three-dimensional (3D) double- and triple-resonance heteronuclear NMR experiments. These studies employ uniformly labeled 15N...

journal_title：Biochemistry

authors： Powers R,Garrett DS,March CJ,Frieden EA,Gronenborn AM,Clore GM

更新日期：1992-05-05 00:00:00

abstract：:In previous studies [van Wijk, K. J., Bingsmark, S., Aro, E.-M., & Andersson, B. (1995) J. Biol. Chem. 270, 25685-25695; van Wijk, K. J., Andersson, B., & Aro, E.-M. (1996) J. Biol. Chem 271, 9627-9636], we have demonstrated that D1 protein synthesized in isolated chloroplasts and thylakoids is incorporated into the p...

journal_title：Biochemistry

authors： van Wijk KJ,Roobol-Boza M,Kettunen R,Andersson B,Aro EM

更新日期：1997-05-20 00:00:00

abstract：:β-Lactamase inhibitors (BLIs) restore the efficacy of otherwise obsolete β-lactams. However, commercially available BLIs are not effective against metallo-β-lactamases (MBLs), which continue to be disseminated globally. One group of the most clinically important MBLs is the VIM family. The discovery of VIM-24, a natur...

journal_title：Biochemistry

authors： Mojica MF,Mahler SG,Bethel CR,Taracila MA,Kosmopoulou M,Papp-Wallace KM,Llarrull LI,Wilson BM,Marshall SH,Wallace CJ,Villegas MV,Harris ME,Vila AJ,Spencer J,Bonomo RA

更新日期：2015-05-26 00:00:00

abstract：:Phospholipids containing a saturated fatty acid in the primary position and an unsaturated fatty acid in the secondary position are a major structural part of biological membranes. The mixed-chain hydrophobic core of the membranes is the diacylglycerol part. To better understand the core properties of membranes we hav...

journal_title：Biochemistry

authors： Di L,Small DM

更新日期：1995-12-26 00:00:00

abstract：:The gene encoding enzyme IIA(glc) (EIIA) of the phosphoenolpyruvate:sugar phosphotransferase system of Mycoplasma capricolum was cloned into a regulated expression vector. The purified protein product of the overexpressed gene was characterized as an active phosphoacceptor from HPr with a higher pI than previously des...

journal_title：Biochemistry

authors： Zhu PP,Nosworthy N,Ginsburg A,Miyata M,Seok YJ,Peterkofsky A

更新日期：1997-06-10 00:00:00

abstract：:Chymopapain, a cysteine protease of papaya latex, has been purified with the use of fast protein liquid chromatography. Two homogeneous fractions were analyzed for thiol content and thiol reactivity. It was found that peak 1 and peak 2 contained two and three thiol groups, respectively, per mole of enzyme. This result...

journal_title：Biochemistry

authors： Kóródi I,Asbóth B,Polgár L

更新日期：1986-11-04 00:00:00

abstract：:There are 10 transmembrane protein (TMEM) 16-family proteins in humans and mice. Among them, TMEM16F acts as a Ca(2+)-dependent phospholipid scramblase at the plasma membrane. However, how Ca(2+) activates TMEM16F's phospholipid-scramblase activity has not been elucidated. Here we found that in the presence of Ca(2+),...

journal_title：Biochemistry

authors： Ishihara K,Suzuki J,Nagata S

更新日期：2016-06-14 00:00:00

abstract：:Mismatches in DNA occur naturally during replication and as a result of endogenous DNA damaging agents, but the mismatch repair (MMR) pathway acts to correct mismatches before subsequent rounds of replication. Rhodium metalloinsertors bind to DNA mismatches with high affinity and specificity and represent a promising ...

journal_title：Biochemistry

authors： Ernst RJ,Komor AC,Barton JK

更新日期：2011-12-20 00:00:00

abstract：:Our previous studies demonstrated that the first 1000 amino acid residues (the βα1 domain) of human apolipoprotein (apo) B-100, termed apoB:1000, are required for the initiation of lipoprotein assembly and the formation of a monodisperse stable phospholipid (PL)-rich particle. The objectives of this study were (a) to ...

journal_title：Biochemistry

authors： Manchekar M,Kapil R,Sun Z,Segrest JP,Dashti N

更新日期：2017-08-08 00:00:00

abstract：:A metastable intermediate was found in the course of the denaturation of purified cytochrome P450 2B1 by increasing concentrations of guanidine hydrochloride (GuHCl). The metastable intermediate has no or low absorbance at 450 nm in the reduced carbon monoxide difference spectrum and has no absorbance at 420 nm. The i...

journal_title：Biochemistry

authors： Yu XC,Shen S,Strobel HW

更新日期：1995-04-25 00:00:00

abstract：:Tissue plasminogen activator-inhibitor complexes were purified from the conditioned medium of human umbilical vein endothelial cells by affinity chromatography followed by gel filtration. It was found that a single complex was isolated which can exist in two distinct interconvertible conformations. These may be separa...

journal_title：Biochemistry

authors： Sanzo MA,Marasa JC,Wittwer AJ,Siegel NR,Harakas NK,Feder J

更新日期：1987-11-17 00:00:00

abstract：:The bacterial phosphoenolpyruvate:sugar phosphotransferase system involves a series of reactions in which phosphoprotein intermediates are formed. Histidine-containing protein (HPr) is phosphorylated on the N delta 1 position of the imidazole ring of His15 by enzyme I and acts as a phosphoryl donor to the sugar-specif...

journal_title：Biochemistry

authors： Rajagopal P,Waygood EB,Klevit RE

更新日期：1994-12-27 00:00:00

abstract：:Nitridomanganese(V) protoporphyrin IX was prepared by hypochlorite oxidation of the corresponding manganese(III) protoporphyrin IX derivative in the presence of ammonium ion and by photolysis of the corresponding azidomanganese(III) complex. Myoglobin and horseradish peroxidase containing this novel protoporphyrin der...

journal_title：Biochemistry

authors： Tsubaki M,Hori H,Hotta T,Hiwatashi A,Ichikawa Y,Yu NT

更新日期：1987-08-11 00:00:00

abstract：:The interaction between skeletal myosin subfragment 1 (S1) and filamentous actin was examined at various intermediate states of the actomyosin ATPase cycle by chemical cross-linking experiments. Reaction of the actin-S1 complex with 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide and N-hydroxysuccinimide generated pro...

journal_title：Biochemistry

authors： Van Dijk J,Fernandez C,Chaussepied P

更新日期：1998-06-09 00:00:00

abstract：:The regulatory (R) subunit of cAMP-dependent protein kinase (cAPK) is a multidomain protein with two tandem cAMP-binding domains, A and B. The importance of cAMP binding on the stability of the R subunit was probed by intrinsic fluorescence and circular dichroism (CD) in the presence and absence of urea. Several mutan...

journal_title：Biochemistry

authors： Cànaves JM,Leon DA,Taylor SS

更新日期：2000-12-12 00:00:00

abstract：:A study of the dynamics and mechanism of the various thermotropic phase transitions undergone by the hydrated monoacylglycerides monoolein and monoelaidin, in the temperature range of 20-120 degrees C and from 0 to 5 M NaCl, has been undertaken. Measurements were made by using time-resolved X-ray diffraction at the Co...

journal_title：Biochemistry

authors： Caffrey M

更新日期：1987-10-06 00:00:00

abstract：:The conformational analysis of non-native states of proteins remains one of the most difficult problems in structural biology, because such states are represented by a superimposition of several states that are rapidly interconverting. Hence, model building of the conformational ensemble remains challenging, although ...

journal_title：Biochemistry

authors： Sziegat F,Silvers R,Hähnke M,Jensen MR,Blackledge M,Wirmer-Bartoschek J,Schwalbe H

更新日期：2012-04-24 00:00:00

abstract：:Heme oxygenase (HO) catalyzes the oxidative cleavage of heme to biliverdin by utilizing O(2) and NADPH. HO (apoHO) was crystallized as twinned P3(2) with three molecules per asymmetric unit, and its crystal structure was determined at 2.55 A resolution. Structural comparison of apoHO and its complex with heme (HO-heme...

journal_title：Biochemistry

authors： Sugishima M,Sakamoto H,Kakuta Y,Omata Y,Hayashi S,Noguchi M,Fukuyama K

更新日期：2002-06-11 00:00:00

abstract：:We have studied the photoreaction of 8-methoxypsoralen (8-MOP), 4,5',8-trimethylpsoralen (TMP), and 4'-(hydroxymethyl)-4,5',8-trimethylpsoralen (HMT) with a pair of 18-base-long oligonucleotides in which a 14-base region is complementary. Only one 5'TpA site, favored for both monoadduct and cross-link formation with p...

journal_title：Biochemistry

authors： Yeung AT,Jones BK,Chu CT

更新日期：1988-05-03 00:00:00

abstract：:This study was undertaken to identify the alpha-helical domains of human apoE that mediate cellular cholesterol efflux and HDL assembly via ATP-binding cassette transporter A1 (ABCA1). The C-terminal (CT) domain (residues 222-299) of apoE was found to stimulate ABCA1-dependent cholesterol efflux in a manner similar to...

journal_title：Biochemistry

authors： Vedhachalam C,Narayanaswami V,Neto N,Forte TM,Phillips MC,Lund-Katz S,Bielicki JK

更新日期：2007-03-13 00:00:00

abstract：:Chemical derivatization studies have been directed at the sulfhydryl group of D-beta-hydroxybutyrate dehydrogenase, a lipid-requiring enzyme. Reaction with N-ethylmaleimide leads to progressive and parallel loss of both enzymic activity and coenzyme binding. Both functions are lost when 1 equiv of sulfhydryl is deriva...

journal_title：Biochemistry

authors： Latruffe N,Brenner SC,Fleischer S

更新日期：1980-11-11 00:00:00

abstract：:Sulfation is a Golgi-specific modification of secretory proteins. We have characterized the proteins that are labeled with 35SO4 in cultures of rat hepatocytes and studied their transport to the medium. Analysis by polyacrylamide gel electrophoresis showed that of the five most heavily labeled proteins, four had well-...

journal_title：Biochemistry

authors： von Würtemberg MM,Fries E

更新日期：1989-05-02 00:00:00

abstract：:Aminoglycoside 2''-phosphotransferase IVa [APH(2'')-IVa] is a member of a family of bacterial enzymes responsible for medically relevant resistance to antibiotics. APH(2'')-IVa confers high-level resistance against several clinically used aminoglycoside antibiotics in various pathogenic Enterococcus species by phospho...

journal_title：Biochemistry

authors： Shi K,Houston DR,Berghuis AM

更新日期：2011-07-19 00:00:00

abstract：:The Cry1Ab toxin produced by Bacillus thuringiensis (Bt) exerts insecticidal action upon binding to BT-R1, a cadherin receptor localized in the midgut epithelium of the tobacco hornworm Manduca sexta [Dorsch, J. A., Candas, M., Griko, N. B., Maaty, W. S., Midboe, E. G., Vadlamudi, R. K., and Bulla, L. A., Jr. (2002) C...

journal_title：Biochemistry

authors： Griko NB,Rose-Young L,Zhang X,Carpenter L,Candas M,Ibrahim MA,Junker M,Bulla LA Jr

更新日期：2007-09-04 00:00:00

abstract：:The interaction of bleomycin A2 with DNA has been examined by fluorescence spectroscopy and proton magnetic resonance techniques. Fluorescence bands observed at 353 and 405 nm in the spectrum of bleomycin were assigned to the bithiazole and 4-aminopyrimidine rings, respectively. Quenching of bithiazole fluorescence by...

journal_title：Biochemistry

authors： Chien M,Grollman AP,Horwitz SB

更新日期：1977-08-09 00:00:00

abstract：:Several enzymatic and chemical reagents were used to probe the secondary structure of Saccharomyces cerevisiae nuclear RNase P RNA in the presence and absence of its protein components. Double-stranded regions were detected with RNase V1 and single-stranded regions with RNase ONE (Escherichia coli RNase I). Nucleotide...

journal_title：Biochemistry

authors： Tranguch AJ,Kindelberger DW,Rohlman CE,Lee JY,Engelke DR

更新日期：1994-02-22 00:00:00

abstract：:Here, we introduce the concept of the "seleno effect" in the study of oxidoreductases that catalyze thiol/disulfide exchange reactions. In these reactions, selenium can replace sulfur as a nucleophile, electrophile, or leaving group, and the resulting change in rate (the seleno effect) is defined as kS/ kSe. In soluti...

journal_title：Biochemistry

authors： O'Keefe JP,Dustin CM,Barber D,Snider GW,Hondal RJ

更新日期：2018-03-20 00:00:00

abstract：:In the dark, 4-azido-2-nitrophenyl phosphate (ANPP) is a phosphate analog which behaves like a simple energy-rich phosphate donor for the sarcoplasmic reticulum (SR) Ca-ATPase. Like p-nitrophenyl phosphate (pNPP), ANPP is hydrolyzed by the enzyme only in the presence of calcium and magnesium (K0.5 and Vmax are 0.3 mM ...

journal_title：Biochemistry

authors： Lacapère JJ,Garin J

更新日期：1994-03-08 00:00:00

abstract：:The ribonucleoprotein ribonuclease P (RNase P) cleaves all tRNA precursors endonucleolitically to produce the mature 5'-end. Dictyostelium discoideum RNase P displays an absolute requirement for Mg2+. Only the alkaline earth cations Ca2+, Sr2+, and Ba2+, under appropriate conditions can substitute to some extent for M...

journal_title：Biochemistry

authors： Tekos A,Stathopoulos C,Drainas D

更新日期：1998-11-03 00:00:00