Disentangling the coil: modulation of conformational and dynamic properties by site-directed mutation in the non-native state of hen egg white lysozyme.

abstract：:Differential scanning calorimetry and CD spectrometry were employed to study the thermal unfolding of light meromyosin (LMM) prepared from carp acclimated to different temperatures. The transition temperatures given by the major peaks at pH 8.0 in 0.6 M KCl for LMM from carp acclimated to 10 degrees C were 32.5 and 39...

journal_title：Biochemistry

authors： Nakaya M,Kakinuma M,Watabe S,Ooi T

更新日期：1997-07-29 00:00:00

abstract：:Inosine 5'-monophosphate (IMP) cyclohydrolase catalyzes the cyclization of 5-formaminoimidazole-4-carboxamide ribonucleotide (FAICAR) to IMP in the final step of de novo purine biosynthesis. Two major types of this enzyme have been discovered to date: PurH in Bacteria and Eukarya and PurO in Archaea. The structure of ...

journal_title：Biochemistry

authors： Kang YN,Tran A,White RH,Ealick SE

更新日期：2007-05-01 00:00:00

abstract：:Isopentenyl phosphate kinase (IPK) catalyzes the phosphorylation of isopentenyl phosphate to form the isoprenoid precursor isopentenyl diphosphate in the archaeal mevalonate pathway. This enzyme is highly homologous to fosfomycin kinase (FomA), an antibiotic resistance enzyme found in a few strains of Streptomyces and...

journal_title：Biochemistry

authors： Mabanglo MF,Serohijos AW,Poulter CD

更新日期：2012-01-31 00:00:00

abstract：:The human ferritin L-chain cDNA was cloned into a vector for overproduction in Escherichia coli, under the regulation of a lambda promoter. The plasmid obtained contains the full L-chain coding region modified at the first two codons. It is able to direct the synthesis of the L-chain which can constitute up to 15% of ...

journal_title：Biochemistry

authors： Levi S,Salfeld J,Franceschinelli F,Cozzi A,Dorner MH,Arosio P

更新日期：1989-06-13 00:00:00

abstract：:The σ1 receptor is an integral membrane protein that shares no homology with other receptor systems, has no unequivocally identified natural ligands, but appears to play critical roles in a wide variety of cell functions. While the number of reports of the possible functions of the σ1 receptor is increasing, almost no...

journal_title：Biochemistry

authors： Brune S,Schepmann D,Klempnauer KH,Marson D,Dal Col V,Laurini E,Fermeglia M,Wünsch B,Pricl S

更新日期：2014-05-13 00:00:00

abstract：:A combination of mutagenesis, calorimetry, kinetics, and small-angle X-ray scattering (SAXS) has been used to study the mechanism of ligand binding energy propagation through human cytochrome P450 reductase (CPR). Remarkably, the energetics of 2',5'-ADP binding to R597 at the FAD-binding domain are affected by mutatio...

journal_title：Biochemistry

authors： Grunau A,Geraki K,Grossmann JG,Gutierrez A

更新日期：2007-07-17 00:00:00

abstract：:The enzyme phosphoenolpyruvate carboxykinase (PEPCK) catalyzes the reversible conversion of oxalacetate and GTP to phosphoenolpyruvate (PEP), GDP, and CO2. PEPCK from higher organisms is a monomer, specifically requires GTP or ITP, and uses Mn2+ as the activating cation. Currently, there is no crystal structure of GTP...

journal_title：Biochemistry

authors： Holyoak T,Nowak T

更新日期：2001-09-18 00:00:00

abstract：:Protein disulfide-isomerase, a protein localized to the lumen of the endoplasmic reticulum of eukaryotic cells, catalyzes the posttranslational formation and rearrangement of protein disulfide bonds. As isolated from bovine liver, the enzyme contains 0.8 free sulfhydryl group per mole of protein monomer and 3.1 disulf...

journal_title：Biochemistry

authors： Gilbert HF

更新日期：1989-09-05 00:00:00

abstract：:HIV-1 Vif is an accessory protein that induces the proteasomal degradation of the host restriction factor, apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3G (APOBEC3G). The N-terminal half of Vif binds to APOBEC3G, and the C-terminal half binds to subunits of a cullin 5-based ubiquitin ligase. This Vi...

journal_title：Biochemistry

authors： Techtmann SM,Ghirlando R,Kao S,Strebel K,Maynard EL

更新日期：2012-03-13 00:00:00

abstract：:CD6 is a member of the scavenger receptor cysteine rich protein superfamily (SRCRSF). This family includes many cell surface proteins whose three-dimensional structures and functions are presently not well understood. The extracellular region of CD6 includes 3 SRCR domains. The membrane proximal SRCR domain specifical...

journal_title：Biochemistry

authors： Bodian DL,Skonier JE,Bowen MA,Neubauer M,Siadak AW,Aruffo A,Bajorath J

更新日期：1997-03-04 00:00:00

abstract：:The quaternary structures of Escherichia coli DNA-dependent RNA polymerase holenzyme (alpha 2 beta beta' sigma) and core enzyme (alpha 2 beta beta') have been investigated by chemical cross-linking with a cleavable bifunctional reagent, methyl 4-mercaptobutyrimidate, and noncleavable reagents, dimethyl suberimidate an...

journal_title：Biochemistry

authors： Hillel Z,Wu CW

更新日期：1977-07-26 00:00:00

abstract：:The interaction between the beta 2 subunit of tryptophan synthase and the coenzyme pyridoxal 5'-phosphate (PLP) is characterized by induced circular dichroism (CD) in the near-UV (260-285 nm) and in the visible region (320-480 nm, extrinsic Cotton effect). Because of its high mean residue ellipticity ([theta] = 56 deg...

journal_title：Biochemistry

authors： Balk H,Merkl I,Bartholmes P

更新日期：1981-10-27 00:00:00

abstract：:The translocation (T) domain of diphtheria toxin plays a critical role in moving the catalytic domain across the endosomal membrane. Translocation/insertion is triggered by a decrease in pH in the endosome where conformational changes of T domain occur through several kinetic intermediates to yield a final trans-membr...

journal_title：Biochemistry

authors： Li J,Rodnin MV,Ladokhin AS,Gross ML

更新日期：2014-11-04 00:00:00

abstract：:We have sought to determine whether insulin can promote cell survival and protect Chinese hamster ovary (CHO) cells from apoptosis induced by serum starvation. Low concentrations of insulin were antiapoptotic for cells overexpressing wild-type insulin receptors but not in cells transfected with kinase-defective insuli...

journal_title：Biochemistry

authors： Lee-Kwon W,Park D,Baskar PV,Kole S,Bernier M

更新日期：1998-11-10 00:00:00

abstract：:The active site of methionyl-tRNA synthetase (MetRS) possesses two functions: synthetic, which provides Met-tRNA for protein synthesis, and editing, which rejects inadvertently misactivated homocysteine. During editing, the side chain -SH group of homocysteine reacts with its activated carboxyl group forming a cyclic ...

journal_title：Biochemistry

authors： Jakubowski H

更新日期：1996-06-25 00:00:00

abstract：:Recent crystal structures of the small ribosomal subunit have made it possible to examine the detailed energetics of codon recognition on the ribosome by computational methods. The binding of cognate and near-cognate anticodon stem loops to the ribosome decoding center, with mRNA containing the Phe UUU and UUC codons,...

journal_title：Biochemistry

authors： Almlöf M,Andér M,Aqvist J

更新日期：2007-01-09 00:00:00

abstract：:The deposition of fibrils and amorphous aggregates of transthyretin (TTR) in patient tissues is a hallmark of TTR amyloid disease, but the molecular details of amyloidogenesis are poorly understood. Tetramer dissociation is typically rate-limiting for TTR amyloid fibril formation, so we have used a monomeric variant o...

journal_title：Biochemistry

authors： Hurshman AR,White JT,Powers ET,Kelly JW

更新日期：2004-06-15 00:00:00

abstract：:Pyruvate decarboxylase (PDC) uses thiamine diphosphate as an essential cofactor to catalyze the formation of acetaldehyde on the pathway of ethanol synthesis. Here we report the crystallographic image of a prereaction intermediate of a bacterial pyruvate decarboxylase prepared by cocrystallizing the enzyme with pyruva...

journal_title：Biochemistry

authors： Pei XY,Erixon KM,Luisi BF,Leeper FJ

更新日期：2010-03-02 00:00:00

abstract：:The size and surface curvature dependence of the properties and stability of single lamellar vesicles have been investigated by using a variety of physicochemical techniques. Dipalmitoylphosphatidylcholine single lamellar vesicles of sizes ranging between 200 and 900 A in diameter have been prepared by the French pres...

journal_title：Biochemistry

authors： Lichtenberg D,Freire E,Schmidt CF,Barenholz Y,Felgner PL,Thompson TE

更新日期：1981-06-09 00:00:00

abstract：:DB2277, a heterocyclic diamidine, is a successful design for mixed base pair (bp) DNA sequence recognition. The compound has a central aza-benzimidazole group that forms two H-bonds with a GC bp that has flanking AT bps. The nuclear magnetic resonance structure of the DB2277-DNA complex with an AAGATA recognition site...

journal_title：Biochemistry

authors： Harika NK,Wilson WD

更新日期：2018-08-21 00:00:00

abstract：:The aspartate receptor is one of the ligand-specific, homodimeric chemoreceptors that detects extracellular attractants and triggers the chemotaxis pathway of Escherichia coli and Salmonella typhimurium. This receptor regulates the activity of the histidine kinase CheA, which forms a kinetically stable complex with th...

journal_title：Biochemistry

authors： Winston SE,Mehan R,Falke JJ

更新日期：2005-09-27 00:00:00

abstract：:We have found that the atomic force microscope (AFM) can be used to image the "beads-on-a-string" chromatin structure in a normal air environment following adsorption onto a cover glass substrate. Individual nucleosome cores and linker DNA could be resolved clearly along chromatin fibers that were reconstituted using ...

journal_title：Biochemistry

authors： Allen MJ,Dong XF,O'Neill TE,Yau P,Kowalczykowski SC,Gatewood J,Balhorn R,Bradbury EM

更新日期：1993-08-24 00:00:00

abstract：:A single amino acid substitution from methionine-184 to valine (M184V) of HIV-1 reverse transcriptase (RT) evokes the 1000-fold 3TC (Lamivudine) resistance by the HIV-1 virus observed in the clinic. The M184V mutant HIV-1 RT was studied to assess its catalytic efficiency during single nucleotide incorporation using a ...

journal_title：Biochemistry

authors： Feng JY,Anderson KS

更新日期：1999-07-20 00:00:00

abstract：:Miniproteins reduce the complexity of the protein-folding problem allowing systematic studies of contributions to protein folding and stabilization. Here, we describe the rational redesign of a miniprotein, PPα, comprising a polyproline II helix, a loop, and an α helix. The redesign provides a de novo framework for in...

journal_title：Biochemistry

authors： Porter Goff KL,Nicol D,Williams C,Crump MP,Zieleniewski F,Samphire JL,Baker EG,Woolfson DN

更新日期：2019-07-16 00:00:00

abstract：:We report an unusually high frequency (543 cm(-)(1)) for an Fe-CO stretching mode in the CO complex of Ascaris suum hemoglobin as compared to vertebrate hemoglobins in which the frequency of the Fe-CO mode is much lower. A second Fe-CO stretching mode in Ascaris hemoglobin is observed at 515 cm(-1). We propose that th...

journal_title：Biochemistry

authors： Das TK,Friedman JM,Kloek AP,Goldberg DE,Rousseau DL

更新日期：2000-02-01 00:00:00

abstract：:Piscidin 4, an antimicrobial peptide recently isolated from mast cells of hybrid striped bass (Morone chrysops female × Morone saxatilis male), is unusual in that it is twice as long (44 amino acids) as the typical members of the piscidin family. We previously showed that native piscidin 4 had a modified amino acid at...

journal_title：Biochemistry

authors： Park NG,Silphaduang U,Moon HS,Seo JK,Corrales J,Noga EJ

更新日期：2011-04-26 00:00:00

abstract：:The single reactive sulfhydryl group, located in the active site of each subunit of dimeric creatine kinase from rabbit muscle (isozyme MM), was selectively labeled with 3-(4-maleimidylphenyl)-7-(diethylamino)-4-methylcoumarin (CPM). Isozyme BB, purified to homogeneity from rabbit brain, was conjugated with the sulfhy...

journal_title：Biochemistry

authors： Grossman SH

更新日期：1983-11-08 00:00:00

abstract：:Recent studies implicate hydrogen sulfide (H2S) oxidation as an important aspect of bacterial antibiotic resistance and sulfide homeostasis. The cst operon of the major human pathogen Staphylococcus aureus is induced by exogenous H2S stress and encodes enzymes involved in sulfide oxidation, including a group I flavopr...

journal_title：Biochemistry

authors： Shen J,Peng H,Zhang Y,Trinidad JC,Giedroc DP

更新日期：2016-11-29 00:00:00

abstract：:Mutagenized dockerin domains of endoglucanase CelD (type I) and of the cellulosome-integrating protein CipA (type II) were constructed by swapping residues 10 and 11 of the first or the second duplicated segment between the two polypeptides. These residues have been proposed to determine the specificity of cohesin-doc...

journal_title：Biochemistry

authors： Schaeffer F,Matuschek M,Guglielmi G,Miras I,Alzari PM,Béguin P

更新日期：2002-02-19 00:00:00

abstract：:Hepatic mixed-function oxidase metabolism of the ubiquitous pollutant polychlorinated biphenyls (PCBs) is implicated in their toxification and detoxification. We used dichlorobiphenyls (DCBs) as models to investigate the effect of the chloro substituent sites on this metabolism experimentally and by molecular orbital ...

journal_title：Biochemistry

authors： Kaminsky LS,Kennedy MW,Adams SM,Guengerich FP

更新日期：1981-12-22 00:00:00