Selective cytotoxicity of rhodium metalloinsertors in mismatch repair-deficient cells.

abstract：:Crk-II is a signaling adaptor protein that is involved in many cellular processes including apoptosis, proliferation, and differentiation. It has a modular domain architecture consisting of an Src homology 2 domain (SH2) followed by two Src homology 3 (SH3) domains. The structures and ligand-binding properties of the ...

journal_title：Biochemistry

authors： Muralidharan V,Dutta K,Cho J,Vila-Perello M,Raleigh DP,Cowburn D,Muir TW

更新日期：2006-07-25 00:00:00

abstract：:Chemosensory cilia isolated from the olfactory epithelium of Rana catesbeiana were solubilized with Lubrol PX in the presence of supplementary lipid, forskolin, and sodium fluoride. Subsequent removal of the detergent by adsorption onto Biobeads SM2 results in the formation of proteoliposomes that display forskolin- a...

journal_title：Biochemistry

authors： Anholt RR

更新日期：1988-08-23 00:00:00

abstract：:We have investigated the interaction of a number of synthetic 20-residue peptides, corresponding to the HA2 N-terminus of the influenza virus hemagglutinin (X31 strain), with phospholipid vesicles and monolayers. Besides the wild-type sequence, two peptides were studied with mutations corresponding to those previously...

journal_title：Biochemistry

authors： Rafalski M,Ortiz A,Rockwell A,van Ginkel LC,Lear JD,DeGrado WF,Wilschut J

更新日期：1991-10-22 00:00:00

abstract：:Common structural motifs, such as the cupin domains, are found in enzymes performing different biochemical functions while retaining a similar active site configuration and structural scaffold. The soil bacterium Bacillus subtilis has 20 cupin genes (0.5% of the total genome) with up to 14% of its genes in the form of...

journal_title：Biochemistry

authors： Gopal B,Madan LL,Betz SF,Kossiakoff AA

更新日期：2005-01-11 00:00:00

abstract：:The active site structures of human Q31 granzyme A, murine granzymes (A, B, C, D, E, and F), and human granzymes (A, B, and 3) isolated from cytotoxic T lymphocytes (CTL) were studied with peptide thioester substrates, peptide chloromethyl ketone, and isocoumarin inhibitors. Human Q31, murine, and human granzyme A hyd...

journal_title：Biochemistry

authors： Odake S,Kam CM,Narasimhan L,Poe M,Blake JT,Krahenbuhl O,Tschopp J,Powers JC

更新日期：1991-02-26 00:00:00

abstract：:Serum paraoxonase hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. High serum paraoxonase levels appear to protect against the neurotoxic effects of organophosphorus substrates of this enzyme [Costa et al. (1990) Toxicol. Appl. Pharmacol. 103, 66-76]. The amino acid sequence accounting f...

journal_title：Biochemistry

authors： Hassett C,Richter RJ,Humbert R,Chapline C,Crabb JW,Omiecinski CJ,Furlong CE

更新日期：1991-10-22 00:00:00

abstract：:A statistical mechanical partition function for phospholipid bilayers is constructed to obtain a theoretical description of the chain melting phase transition in lipid bilayer membranes and of the phase diagrams for two-component bilayers. In addition to providing an accurate representation of the transition temperatu...

journal_title：Biochemistry

authors： Jacobs RE,Hudson BS,Andersen HC

更新日期：1977-10-04 00:00:00

abstract：:The interactions of myelin basic protein and peptides derived from it with detergent micelles of lysophosphatidylglycerol, lysophosphatidylserine, palmitoyllysophosphatidic acid, and sodium lauryl sulfate, and with mixed micelles of the neutral detergent dodecylphosphocholine and the negatively charged detergent palmi...

journal_title：Biochemistry

authors： Mendz GL,Brown LR,Martenson RE

更新日期：1990-03-06 00:00:00

abstract：:The reaction of cytochrome c oxidase with hydrogen peroxide has been of great value in generating and characterizing oxygenated species of the enzyme that are identical or similar to those formed during turnover of the enzyme with dioxygen. Most previous studies have utilized relatively low peroxide concentrations (mi...

journal_title：Biochemistry

authors： Zaslavsky D,Smirnova IA,Brzezinski P,Shinzawa-Itoh K,Yoshikawa S,Gennis RB

更新日期：1999-11-30 00:00:00

abstract：:Endoglucanase Cel6A from Thermobifida fusca hydrolyzes the beta-1,4 linkages in cellulose at accessible points along the polymer. The structure of the catalytic domain of Cel6A from T. fusca in complex with a nonhydrolysable substrate analogue that acts as an inhibitor, methylcellobiosyl-4-thio-beta-cellobioside (Glc(...

journal_title：Biochemistry

authors： Larsson AM,Bergfors T,Dultz E,Irwin DC,Roos A,Driguez H,Wilson DB,Jones TA

更新日期：2005-10-04 00:00:00

abstract：:We have isolated a new toxin, calitoxin (CLX), from the sea anemone Calliactis parasitica whose amino acid sequence differs greatly from that of other sea anemone toxins. The polypeptide chain contains 46 amino acid residues, with a molecular mass of 4886 Da and an isoelectric point at pH 5.4. The amino acid sequence ...

journal_title：Biochemistry

authors： Cariello L,de Santis A,Fiore F,Piccoli R,Spagnuolo A,Zanetti L,Parente A

更新日期：1989-03-21 00:00:00

abstract：:The insulin-linked polymorphic region (ILPR) is a regulatory sequence in the promoter region upstream of the human insulin gene and is widely recognized as a locus of type 1 diabetes susceptibility. Polymorphism of the ILPR sequence can affect expression of both insulin and the adjacent insulin-like growth factor 2 (I...

journal_title：Biochemistry

authors： Wang Y,Zhang H,Ligon LA,McGown LB

更新日期：2009-09-01 00:00:00

abstract：:Fluorescein 5'-isothiocyanate (FITC) was used to modify the lysine residues of bovine transducin (T), a GTP-binding protein involved in phototransduction of rod photoreceptor cells. The incorporation of FITC showed a stoichiometry of approximately 1 mol of FITC/mol of transducin. The labeling was specific for the T al...

journal_title：Biochemistry

authors： Hingorani VN,Ho Y

更新日期：1987-03-24 00:00:00

abstract：:Several bifunctional alkylating agents of the aziridinylbenzoquinone class have been evaluated as potential antitumor agents. 3,6-Bis[(2-hydroxyethyl)amino]-2,5- diaziridinyl-1,4-benzoquinone (BZQ), 2,5-diaziridinyl-1,4-benzoquinone (DZQ), 3,6-bis(carboxyamino)-2,5-diaziridinyl- 1,4-benzoquinone (AZQ), and six analogu...

journal_title：Biochemistry

authors： Hartley JA,Berardini M,Ponti M,Gibson NW,Thompson AS,Thurston DE,Hoey BM,Butler J

更新日期：1991-12-17 00:00:00

abstract：:The DeltaG degrees (N)(-)(D) value obtained from extrapolation to zero denaturant concentration by the linear extrapolation method (LEM) is commonly interpreted to represent the Gibbs energy difference between native (N) and denatured (D) ensembles at the limit of zero denaturant concentration. For DeltaG degrees (N)(...

journal_title：Biochemistry

authors： Bolen DW,Yang M

更新日期：2000-12-12 00:00:00

abstract：:Understanding the molecular mechanisms of enzyme catalysis and allosteric regulation has been a primary goal of biochemistry for many years. The dynamics of these processes, approached through a variety of kinetic methods, are discussed. The results obtained for many different enzymes suggest that multiple intermediat...

journal_title：Biochemistry

authors： Hammes GG

更新日期：2002-07-02 00:00:00

abstract：:Previous work has described the novel ability to modulate in vitro the activity of restriction endonuclease NaeI from Nocardia aerocoligenes by using cleavable DNA and spermidine [Conrad & Topal (1989) Proc. Natl. Acad. Sci. U.S.A. 86, 9707-9711]. In this paper we report the results of a study of 49 type II restrictio...

journal_title：Biochemistry

authors： Oller AR,Vanden Broek W,Conrad M,Topal MD

更新日期：1991-03-05 00:00:00

abstract：:A subgroup of enzymes in the NAD(P)H:FMN reductase family is comprised of flavin reductases from two-component monooxygenase systems. The diverging structural feature in these FMN reductases is a π-helix centrally located at the tetramer interface that is generated by the insertion of an amino acid in a conserved α4 h...

journal_title：Biochemistry

authors： Musila JM,L Forbes D,Ellis HR

更新日期：2018-07-31 00:00:00

abstract：:The structure formed from the DNA oligonucleotide d(G4T4G4) (Oxy-1.5), which contains the Oxytricha telomere repeat T4G4, has been investigated by two-dimensional 1H and 31P NMR spectroscopy. Sequence-specific assignments have been obtained for the 1H and 31P resonances, using a combination of methods including compar...

journal_title：Biochemistry

authors： Smith FW,Feigon J

更新日期：1993-08-24 00:00:00

abstract：:To elucidate the mechanism of solute diffusion through lipid bilayer membranes, nearly 4 ns of molecular dynamics simulations of solutes in phospholipid bilayers was conducted. The study, the first atomic level study of solute diffusion in a lipid bilayer, involved four simulations of an all-atom representation of a f...

journal_title：Biochemistry

authors： Bassolino-Klimas D,Alper HE,Stouch TR

更新日期：1993-11-30 00:00:00

abstract：:Two tetrapeptide sequence homologies between mouse epidermal growth factor precursor (mEGFP) and human follitropin (FSH) were revealed by a computer program that identifies identical residues among polypeptide sequences. The two tetrapeptides, Lys-Thr-Cys-Thr (KTCT) and Thr-Arg-Asp-Leu (TRDL), are present in the hormo...

journal_title：Biochemistry

authors： Sluss PM,Krystek SR Jr,Andersen TT,Melson BE,Huston JS,Ridge R,Reichert LE Jr

更新日期：1986-05-06 00:00:00

abstract：:To reduce ribonucleotides to deoxyribonucleotides, the manganese-bound form of class Ib ribonucleotide reductase (RNR) must be activated via a pathway that involves redox protein(s). The reduced flavoprotein NrdI is an important protein in this pathway, as it reduces dioxygen to superoxide. Superoxide then reacts with...

journal_title：Biochemistry

authors： Lofstad M,Gudim I,Hammerstad M,Røhr ÅK,Hersleth HP

更新日期：2016-09-13 00:00:00

abstract：:CytA is a member of a functionally defined family of insecticidal delta-endotoxins occurring in parasporal crystals of Bacillus thuringiensis var. israelensis. We investigated the ability of CytA to permeabilize the membrane and release fluorescence marker molecules from unilamellar lipid vesicles. Both protoxin (27 k...

journal_title：Biochemistry

authors： Butko P,Huang F,Pusztai-Carey M,Surewicz WK

更新日期：1996-09-03 00:00:00

abstract：:Calcium protects a limited number of embryonic chick neural retina cell surface proteins from tryptic cleavage. One glycoprotein of Mr approximately 1.3 X 10(5) and pI approximately 4.8 (gp130) is present in intact retinas and, in the presence of Ca2+, is both resistant to tryptic cleavage and poorly iodinated. When c...

journal_title：Biochemistry

authors： Cook JH,Pratt RS,Lilien J

更新日期：1984-02-28 00:00:00

abstract：:The molecular mechanism of O2 binding to hemoglobin (Hb) and myoglobin (Mb) is a long-standing issue in the field of bioinorganic and biophysical chemistry. The nature of Fe-O2 bond in oxy Hb and Mb had been extensively investigated by resonance Raman spectroscopy, which assigned the Fe-O2 stretching bands at ∼570 cm-...

journal_title：Biochemistry

authors： Ohta T,Shibata T,Kobayashi Y,Yoda Y,Ogura T,Neya S,Suzuki A,Seto M,Yamamoto Y

更新日期：2018-12-04 00:00:00

abstract：:The Cyt toxins produced by the bacteria Bacillus thuringiensis show insecticidal activity against some insects, mainly dipteran larvae, being able to kill mosquitoes and black flies. However, they also possess a general cytolytic activity in vitro, showing hemolytic activity in red blood cells. These proteins are comp...

journal_title：Biochemistry

authors： Rodriguez-Almazan C,Ruiz de Escudero I,Cantón PE,Muñoz-Garay C,Pérez C,Gill SS,Soberón M,Bravo A

更新日期：2011-01-25 00:00:00

abstract：:The mechanism of DNA polymerase beta-catalyzed nucleotidyl transfer consists of chemical steps involving primer 3' OH deprotonation, nucleophilic attack, and pyrophosphate leaving-group elimination, preceded by dNTP binding which induces a large-amplitude conformational change for Watson-Crick nascent base pairs. Ambi...

journal_title：Biochemistry

authors： Sucato CA,Upton TG,Kashemirov BA,Osuna J,Oertell K,Beard WA,Wilson SH,Florián J,Warshel A,McKenna CE,Goodman MF

更新日期：2008-01-22 00:00:00

abstract：:Aminoacylation of a transfer RNA (tRNA) by its cognate aminoacyl-tRNA synthetase relies upon the recognition of specific nucleotides as well as conformational features within the tRNA by the synthetase. In Escherichia coli, the aminoacylation of tRNA(His) by histidyl-tRNA synthetase (HisRS) is highly dependent upon th...

journal_title：Biochemistry

authors： Connolly SA,Rosen AE,Musier-Forsyth K,Francklyn CS

更新日期：2004-02-03 00:00:00

abstract：:P-Glycoprotein (Pgp) expression in cell lines derived from tumors arising from cells which normally express Pgp can be increased by sodium butyrate and other differentiating agents. Although the Pgp level increased 25-fold after sodium butyrate treatment in SW620 human colon carcinoma cells, the intracellular accumula...

journal_title：Biochemistry

authors： Bates SE,Currier SJ,Alvarez M,Fojo AT

更新日期：1992-07-21 00:00:00

abstract：:In the cyanobacterium Anabaena PCC7120, the precursor to tRNA(Leu) contains a 249-nucleotide group I intron that undergoes efficient self-splicing in vitro. By deleting the 5' and 3' splice sites, this intron has now been converted to an RNA enzyme that uses a guanosine nucleophile to cleave substrate RNAs (S) with mu...

journal_title：Biochemistry

authors： Zaug AJ,Dávila-Aponte JA,Cech TR

更新日期：1994-12-13 00:00:00