Strand orientation in the DNA quadruplex formed from the Oxytricha telomere repeat oligonucleotide d(G4T4G4) in solution.

abstract：:We have prepared the following cadmium-113-substituted derivatives of bovine superoxide dismutase and recorded the nuclear magnetic resonance (NMR) spectrum of the cadmium: 2Cd(II), in which Cd(II) is presumed to bind to the Zn(II) site and the copper site is unoccupied, and 2Cd(II)--2Cu(I), which is analogous to the ...

journal_title：Biochemistry

authors： Bailey DB,Ellis PD,Fee JA

更新日期：1980-02-05 00:00:00

abstract：:The folding of many globular proteins from the unfolded (U) to the native (N) state appears to be describable by a two-state N ↔ U model, which has led to the general belief that protein folding occurs in a highly cooperative manner. One reason for the widespread belief in "two-state folding" is that protein folding r...

journal_title：Biochemistry

authors： Jethva PN,Udgaonkar JB

更新日期：2018-10-09 00:00:00

abstract：:To understand relationships between membrane-binding properties of cytolytic peptides and resulting cytotoxicity, we investigated interactions of dermaseptin analogues with model bilayers by means of surface plasmon resonance. First, we tested the system by comparing two native dermaseptins, S1 and S4, whose binding p...

journal_title：Biochemistry

authors： Gaidukov L,Fish A,Mor A

更新日期：2003-11-11 00:00:00

abstract：:Insulin-stimulated glucose disposal in skeletal muscle proceeds predominantly through a nonoxidative pathway with glycogen synthase as a rate-limiting enzyme, yet the mechanisms for insulin activation of glycogen synthase are not understood despite years of investigation. Isolation of putative insulin second messenger...

journal_title：Biochemistry

authors： Brautigan DL,Brown M,Grindrod S,Chinigo G,Kruszewski A,Lukasik SM,Bushweller JH,Horal M,Keller S,Tamura S,Heimark DB,Price J,Larner AN,Larner J

更新日期：2005-08-23 00:00:00

abstract：:Apolipoprotein A-I (apoA-I) is the main protein of plasma high-density lipoproteins (HDL, or good cholesterol) that remove excess cell cholesterol and protect against atherosclerosis. In hereditary amyloidosis, mutations in apoA-I promote its proteolysis and the deposition of the 9-11 kDa N-terminal fragments as fibri...

journal_title：Biochemistry

authors： Gursky O,Mei X,Atkinson D

更新日期：2012-01-10 00:00:00

abstract：:The ATP analogue 5'-[p-(fluorosulfonyl)benzoyl]adenosine (FSBA) was used to chemically modify the ATP binding sites of the carbamyl phosphate synthetase domain of CAD, the multifunctional protein that catalyzes the first steps in mammalian pyrimidine biosynthesis. Reaction of CAD with FSBA resulted in the inactivation...

journal_title：Biochemistry

authors： Kim HS,Lee L,Evans DR

更新日期：1991-10-22 00:00:00

abstract：:The thermotropic behavior of 20 chemically related glycosphingolipids (GSLs) of high purity, containing neutral and anionic carbohydrate residues in their oligosaccharide chains, was studied by high-sensitivity differential scanning calorimetry. In general, the polar head group of GSLs appears to be one of the major d...

journal_title：Biochemistry

authors： Maggio B,Ariga T,Sturtevant JM,Yu RK

更新日期：1985-02-26 00:00:00

abstract：:Fluorescence correlation spectroscopy (FCS) allows the study of interactions of fluorescently labeled ligand with receptors in living cells at single-molecule detection sensitivity. From the autocorrelation functions of fluorescence intensity fluctuations, the diffusion time of molecules through the confocal volume is...

journal_title：Biochemistry

authors： Pramanik A,Olsson M,Langel U,Bartfai T,Rigler R

更新日期：2001-09-11 00:00:00

abstract：:AhpF, the flavoprotein reductase component of the Salmonella typhimurium alkyl hydroperoxide reductase system, catalyzes the reduction of an intersubunit disulfide bond in the peroxidatic active site of the system's other component, AhpC, a member of the peroxiredoxin family. Previous studies have shown that AhpF can ...

journal_title：Biochemistry

authors： Reynolds CM,Poole LB

更新日期：2001-04-03 00:00:00

abstract：:Arylamine N-acetyltransferases (NATs) catalyze an acetyl group transfer from acetyl coenzyme A (AcCoA) to arylamines, hydrazines, and their N-hydroxylated arylamine metabolites. The recently determined three-dimensional structures of prokaryotic NATs have revealed a cysteine protease-like Cys-His-Asp catalytic triad, ...

journal_title：Biochemistry

authors： Wang H,Vath GM,Gleason KJ,Hanna PE,Wagner CR

更新日期：2004-06-29 00:00:00

abstract：:Ultraviolet absorption techniques were used to study the thermodynamics of duplex formation for a DNA decamer, d(GCGAAAAGCG).d(CGCTTTTCGC), and a series of related duplexes, each of which contains a bulged base centered in the A.T tract. Thermodynamic parameters were obtained from nonlinear least-squares fits of the m...

journal_title：Biochemistry

authors： LeBlanc DA,Morden KM

更新日期：1991-04-23 00:00:00

abstract：:A common motif in protein structures is the assembly of alpha-helices. Natural alpha-helical assemblies, such as helical bundles and coiled coils, consist of multiple right-handed alpha-helices. Here we design a protein complex containing both left-handed and right-handed helices, with peptides of D- and L-amino acids...

journal_title：Biochemistry

authors： Sia SK,Kim PS

更新日期：2001-07-31 00:00:00

abstract：:Primase is an essential DNA replication enzyme in Escherichia coli and responsible for primer synthesis during lagging strand DNA replication. Although the interaction of primase with single-stranded DNA plays an important role in primer RNA and Okazaki fragment synthesis, the mechanism of DNA binding and site selecti...

journal_title：Biochemistry

authors： Khopde S,Biswas EE,Biswas SB

更新日期：2002-12-17 00:00:00

abstract：:The interaction of proteoglycan monomers with hyaluronate in cartilage is mediated by a globular binding region at the N-terminus of the proteoglycan monomer; this interaction is stabilized by link protein. Sequences show that both the binding region (27% carbohydrate) and the link protein (6% carbohydrate) contain an...

journal_title：Biochemistry

authors： Perkins SJ,Nealis AS,Dunham DG,Hardingham TE,Muir IH

更新日期：1991-11-05 00:00:00

abstract：:Trypsin cleavage has been used to probe structure-function relationships of the Escherichia coli ATP synthase (ECF1F0). Trypsin cleaved all five subunits, alpha, beta, gamma, delta, and epsilon, in isolated ECF1. Cleavage of the alpha subunit involved the removal of the N-terminal 15 residues, the beta subunit was cle...

journal_title：Biochemistry

authors： Gavilanes-Ruiz M,Tommasino M,Capaldi RA

更新日期：1988-01-26 00:00:00

abstract：:Traditional antibody generation, using either phage display or animal immunization, relies on purified antigens. Many membrane proteins, such as G protein-coupled receptors, solute carriers, or ion channels, are important drug targets but very challenging for the formation of antibodies due to the difficulty of protei...

journal_title：Biochemistry

authors： Chang YC,Kao CY,Tang HC,Huang MS,Mou KY

更新日期：2020-11-10 00:00:00

abstract：:Sir2 protein deacetylases (or sirtuins) catalyze NAD+-dependent conversion of epsilon-amino-acetylated lysine residues to deacetylated lysine, nicotinamide, and 2'-O-acetyl-ADP-ribose. Small-molecule modulation of sirtuin activity might treat age-associated diseases, such as type II diabetes, obesity, and neurodegener...

journal_title：Biochemistry

authors： Smith BC,Denu JM

更新日期：2007-12-18 00:00:00

abstract：:In this work we modeled the circular dichroism (CD) spectrum of LHCII, the main light harvesting antenna of photosystem II of higher plants. Excitonic calculations are performed for a monomeric subunit, taken from the crystal structure of trimeric LHCII from spinach [Liu, Z. F., Yan, H. C., Wang, K. B., Kuang, T. Y., ...

journal_title：Biochemistry

authors： Georgakopoulou S,van der Zwan G,Bassi R,van Grondelle R,van Amerongen H,Croce R

更新日期：2007-04-24 00:00:00

abstract：:The effects of ionophore A23187 on the movements of Ca2+, Mg2+, H+ phosphate, and succinate and its effects on energy utilization by mitochondria have been studied as a function of ionophore concentrations. At a low ratio of the compound to mitochondrial protein, below that required for maximal uncoupling, an apparent...

journal_title：Biochemistry

authors： Pfeiffer DR,Hutson SM,Kauffman RF,Lardy HA

更新日期：1976-06-15 00:00:00

abstract：:The N-terminal extracellular parts of human G-protein coupled receptor class B, for example, receptors for secretin, glucagon, or parathyroid hormone, are involved in ligand binding. To obtain structural and functional information on the N-terminal receptor fragment of human parathyroid hormone receptor 1 (PTHR1), the...

journal_title：Biochemistry

authors： Grauschopf U,Lilie H,Honold K,Wozny M,Reusch D,Esswein A,Schäfer W,Rücknagel KP,Rudolph R

更新日期：2000-08-01 00:00:00

abstract：:The number of self-cleaving ribozymes has increased sharply in recent years, giving rise to elaborations of the four known ribozyme catalytic strategies, α, β, γ, and δ. One such extension is utilized by the twister ribozyme, which is hypothesized to conduct δ, or general acid catalysis, via N3 of the syn adenine +1 n...

journal_title：Biochemistry

authors： Messina KJ,Kierzek R,Tracey MA,Bevilacqua PC

更新日期：2019-12-03 00:00:00

abstract：:The derivatives of calf thymus DNA in which n-butylamine is covalently attached as described in the preceding paper in this series [Chen, C. Y., Pheiffer, B. H., Zimmerman, S. B., & Hanlon, S. (1983) Biochemistry (preceding paper in this issue)] were examined by Raman spectroscopy. As previously mentioned, these compl...

journal_title：Biochemistry

authors： Fish SR,Chen CY,Thomas GJ Jr,Hanlon S

更新日期：1983-09-27 00:00:00

abstract：:The di-heme enzyme MauG catalyzes the oxidative biosynthesis of a tryptophan tryptophylquinone cofactor on a precursor of the enzyme methylamine dehydrogenase (preMADH). Reaction of H2O2 with the diferric form of MauG, or reaction of O2 with diferrous MauG, forms the catalytic intermediate known as bis-Fe(IV), which a...

journal_title：Biochemistry

authors： Yukl ET,Williamson HR,Higgins L,Davidson VL,Wilmot CM

更新日期：2013-12-31 00:00:00

abstract：:We obtained a novel carbohydrate-binding peptide having a helix-loop-helix scaffold from a random peptide library. The helix-loop-helix peptide library randomized at five amino acid residues was displayed on the major coat protein of a filamentous phage. Affinity selection with a ganglioside, Galbeta1-3GalNAcbeta1-4(N...

journal_title：Biochemistry

authors： Matsubara T,Iida M,Tsumuraya T,Fujii I,Sato T

更新日期：2008-07-01 00:00:00

abstract：:A panel of 10 hybridomas was assembled to assess the influence of various genetic and biological factors upon glycosylation of secreted monoclonal IgG. After exhaustive Pronase digestion of IgG, glycopeptides were characterized chromatographically by apparent size, charge, and concanavalin A (Con A)-Sepharose and Lens...

journal_title：Biochemistry

authors： Rothman RJ,Warren L,Vliegenthart JF,Härd KJ

更新日期：1989-02-07 00:00:00

abstract：:DNA-binding geometry and dynamics of a number of anthracyclines, including adriamycin and 4-demethoxydaunorubicin, interacting with DNA have been studied by means of linear dichroism and fluorescence techniques. The anthracycline chromophore is found to be approximately parallel to the plane of the DNA bases and to ha...

journal_title：Biochemistry

authors： Eriksson M,Nordén B,Eriksson S

更新日期：1988-10-18 00:00:00

abstract：:The gamma-phosphoryl groups of two intermediates (M-ATP and M-ADP-P1) in the pathway of MgATP hydrolysis by myosin undergo extensive oxygen exchange with water. Actin activates the overall rate of hydrolysis at a rate-limiting step which follows these exchange reactions. Thus, actin, by decreasing the turnover time of...

journal_title：Biochemistry

authors： Shukla KK,Levy HM

更新日期：1977-01-11 00:00:00

abstract：:Activation enthalpies and entropies are reported for proton-deuteron exchange at 42 amide sites in T4 lysozyme and compared with activation volumes for the same residues obtained earlier [Hitchens, T. K., and Bryant, R. G. (1998) Biochemistry 37, 5878-5887]. There is no correlation found between activation volume and ...

journal_title：Biochemistry

authors： Dixon ME,Hitchens TK,Bryant RG

更新日期：2000-01-11 00:00:00

abstract：:Inhibition of the yeast F(0)F(1)-ATP synthase by the regulatory peptides IF1 and STF1 was studied using intact mitochondria and submitochondrial particles from wild-type cells or from mutants lacking one or both peptides. In intact mitochondria, endogenous IF1 only inhibited uncoupled ATP hydrolysis and endogenous STF...

journal_title：Biochemistry

authors： Venard R,Brèthes D,Giraud MF,Vaillier J,Velours J,Haraux F

更新日期：2003-06-24 00:00:00

abstract：:The biosynthesis of the C ring of the antitumor antibiotic agent, tomaymycin, is proposed to proceed through five enzyme-catalyzed steps from l-tyrosine. The genes encoding these enzymes have recently been cloned and their functions tentatively assigned, but there is limited biochemical evidence supporting the assignm...

journal_title：Biochemistry

authors： Burks EA,Yan W,Johnson WH Jr,Li W,Schroeder GK,Min C,Gerratana B,Zhang Y,Whitman CP

更新日期：2011-09-06 00:00:00