Abstract:
:Trypsin cleavage has been used to probe structure-function relationships of the Escherichia coli ATP synthase (ECF1F0). Trypsin cleaved all five subunits, alpha, beta, gamma, delta, and epsilon, in isolated ECF1. Cleavage of the alpha subunit involved the removal of the N-terminal 15 residues, the beta subunit was cleaved near the C-terminus, the gamma subunit was cleaved near Ser202, and the delta and epsilon subunits appeared to be cleaved at several sites to yield small peptide fragments. Trypsin cleavage of ECF1 enhanced the ATPase activity between 6- and 8-fold in different preparations, in a time course that followed the cleavage of the epsilon subunit. This removal of the epsilon subunit increased multisite ATPase activity but not unisite ATPase activity, showing that the inhibitory role of the epsilon subunit is due to an effect on cooperativity. The detergent lauryldimethylamine oxide was found to increase multisite catalysis and also increase unisite catalysis more than 2-fold. Prolonged trypsin cleavage left a highly active ATPase containing only the alpha and beta subunits along with two fragments of the gamma subunit. All of the subunits of ECF1 were cleaved by trypsin in preparations of ECF1F0 at the same sites as in isolated ECF1. Two subunits, the beta and epsilon subunits, were cleaved at the same rate in ECF1F0 as in ECF1 alone. The alpha, gamma, and delta subunits were cleaved significantly more slowly in ECF1F0.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Biochemistryjournal_title
Biochemistryauthors
Gavilanes-Ruiz M,Tommasino M,Capaldi RAdoi
10.1021/bi00402a016subject
Has Abstractpub_date
1988-01-26 00:00:00pages
603-9issue
2eissn
0006-2960issn
1520-4995journal_volume
27pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::To identify regions of the Torpedo Na,K-ATPase alpha-subunit that interact with membrane lipid and to characterize conformationally dependent structural changes in the transmembrane domain, we have proteolytically mapped the sites of photoincorporation of the hydrophobic compounds 3-(trifluoromethyl)-3-(m-[(125)I]iodo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0015461
更新日期:2000-11-07 00:00:00
abstract::Since a major function of RNA polymerase must be to bring together substrates in the optimal configuration for internucleotide bond formation, studies have been undertaken to understand the geometrical relationship of the two substrates. A model has been constructed for the geometry of interaction of two ATP molecules...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00477a017
更新日期:1990-06-26 00:00:00
abstract::ArsR (or ArsR/SmtB) family metalloregulatory homodimeric repressors collectively respond to a wide range of metal ion inducers in regulating homeostasis and resistance of essential and nonessential metal ions in bacteria. BxmR from the cyanobacterium Osciliatoria brevis is the first characterized ArsR protein that sen...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi801313y
更新日期:2008-10-07 00:00:00
abstract::HcbA1 is a unique flavoenzyme that catalyzes the first step in the bacterial hexachlorobenzene catabolic pathway. Here we report in vitro reconstitution of the HcbA1-catalyzed reaction. Detailed mechanistic studies provide evidence for nucleophilic aromatic substitution and flavin-N5-oxide formation. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b00012
更新日期:2019-03-05 00:00:00
abstract::The PLL(PTE-like lactonase)-group of enzymes within the amidohydrolase superfamily hydrolyze N-acyl-homoserine lactones (AHLs) that are involved in bacterial quorum-sensing pathways. These enzymes possess the (beta/alpha)(8)-barrel fold and serve as attractive templates for in vitro evolution and engineering of quorum...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9004045
更新日期:2009-05-26 00:00:00
abstract::Cyclobutane pyrimidine dimers (Pyr < > Pyr) are the major DNA photoproducts induced by the UV component of solar radiation. Photoreactivating enzyme (DNA photolyase) repairs DNA by utilizing the energy of visible light to break the cyclobutane ring of the dimer. Photolyases are monomeric proteins of 50-60 kDa with sto...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/bi00167a001
更新日期:1994-01-11 00:00:00
abstract::The interaction of bovine spleen profilin with ATP- and ADP-G-actin and poly(L-proline) has been studied by spectrofluorimetry, analytical ultracentrifugation, and rapid kinetics in low ionic strength buffer. Profilin binding to G-actin is accompanied by a large quenching of tryptophan fluorescence, allowing the measu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00194a011
更新日期:1994-07-19 00:00:00
abstract::Studies on the structure-function relationship of transporters require the availability of sufficient amounts of the protein in a functional state. In this paper, we report the functional expression, purification, and reconstitution of the human sodium/d-glucose cotransporter1 (hSGLT1) in Pichia pastoris and ligand-in...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi051377q
更新日期:2005-11-29 00:00:00
abstract::The phosphatidylcholine-preferring phospholipase C from Bacillus cereus (PLCBc) is a 28.5 kDa enzyme with three zinc ions in its active site. Although much is known about the roles that various PLCBc active site amino acids play in binding and catalysis, there is little information about the rate-determining step of t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9821216
更新日期:1999-04-06 00:00:00
abstract::CRISPR-Cas9 technology has been at the forefront of the field of biology. The Streptococcus pyogenes (SpyCas9) protein forms a complex with guide RNA and can recognize and cleave double-stranded DNA through hybridization based on 20 base pairings. SpyCas9 has two nuclease domains, HNH and RuvC, each of which cuts each...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.0c00766
更新日期:2021-01-26 00:00:00
abstract::We demonstrate a general strategy for determining structures from showers of microcrystals. It uses acoustic droplet ejection to transfer 2.5 nL droplets from the surface of microcrystal slurries, through the air, onto mounting micromesh pins. Individual microcrystals are located by raster-scanning a several-micromete...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200549x
更新日期:2011-05-31 00:00:00
abstract::Onconase (ONC) from Rana pipiens is the smallest member of the ribonuclease A (RNase A) superfamily. Despite a tertiary structure similar to RNase A, ONC is distinguished by an extremely high thermodynamic stability. In the present paper we have probed the significance of three structural regions, which exhibit struct...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0525223
更新日期:2006-03-21 00:00:00
abstract::The specificity of trypsin for Arg- and Lys-containing substrates depends upon the presence of Asp189 at the base of the primary binding pocket. The crystal structure of anionic rat trypsin D189S complexed with BPTI reveals that removal of the aspartate side chain permits the binding of a well-ordered acetate ion in a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00177a016
更新日期:1994-03-22 00:00:00
abstract::Deuterium nuclear magnetic resonance spectra of dimyristoylphosphatidylcholines specifically labeled in positions 2', 3', 4', 6', 8', 10', 12', and 14', of the 2 chain, of an N-deuteriomethylphosphatidylcholine, and of cholesterol-3alpha-d1, have been obtained by the Fourier transform method at 5.46 and 3.52 T on two ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00607a006
更新日期:1978-07-11 00:00:00
abstract::Two extremely potent inhibitors of citrate synthase, carboxyl and primary amide analogues of acetyl coenzyme A, have been synthesized. The ternary complexes of these inhibitors with oxaloacetate and citrate synthase have been crystallized and their structures analyzed at 1.70- and 1.65-A resolution, respectively. The ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00191a002
更新日期:1994-06-28 00:00:00
abstract::NMR studies of protein denatured states provide insights into potential initiation sites for folding that may be too transient to be observed kinetically. We have characterized the structure and dynamics of the acid-denatured state of protein G by using a F30H mutant of G(B1) which is on the margin of stability. At 5 ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9920230
更新日期:2000-02-08 00:00:00
abstract::Tk-subtilisin from the hyperthermophiolic archaeon Thermococcus kodakaraensis matures from Pro-Tk-subtilisin upon autoprocessing and degradation of Tk-propeptide [Tanaka, S., Saito, K., Chon, H., Matsumura, H., Koga, Y., Takano, K., and Kanaya, S. (2007) J. Biol. Chem. 282, 8246-8255]. It requires Ca(2+) for folding a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi901334b
更新日期:2009-11-10 00:00:00
abstract::Studies were undertaken to assess the postulated involvement of subunit III in the proton-linked functions of cytochrome c oxidase. The effect of pH on the steady-state kinetic [corrected] parameters of subunit III containing and subunit III depleted cytochrome oxidase was determined by using beef heart and rat liver ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00417a016
更新日期:1988-08-23 00:00:00
abstract::The ribosome-dependent stringent factor reaction was found to be nonspecific with regard to the number of phosphate groups linked to the 5' position of guanosine nucleotides. Both GMP and guanosine 5'-tetraphosphate could accept a pyrophosphoryl group from ATP although at a much lower rate than GDP or GTP. Guanosine 5...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00676a015
更新日期:1975-03-11 00:00:00
abstract::It has been suggested that the down-regulation of hepatic HMG-CoA reductase by dietary cholesterol requires modification of the cholesterol molecule before it can exert its suppressive action. In a recent study [Lund, E., Breuer, O., & Björkhem, I. (1992) J. Biol. Chem. 267, 25092-25097], we showed that side-chain hyd...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00167a038
更新日期:1994-01-11 00:00:00
abstract::N-Terminal glutaminyl and glutamyl residues of peptides and proteins tend to form pyroglutamic acid (pGlu) by intramolecular cylization. The rate constants for spontaneous cyclization of glutamine (10(-6) s(-1)) and glutamic acid (10(-9) s(-1)) in aqueous solution differ by approximately 3 orders of magnitude at pH 6....
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9018835
更新日期:2009-12-22 00:00:00
abstract::Isoforms of transforming growth factor beta (TGFbeta) are 25 kDa homodimeric polypeptides that signal by binding and bringing together two related, functionally distinct cell surface receptors designated as TbetaR1 and TbetaR2. Here, we report the solution structure of the 13.8 kDa extracellular domain of human TbetaR...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034366a
更新日期:2003-09-02 00:00:00
abstract::The mitochondrial folate transporter (MFT) was previously identified in human and hamster cells. Sequence homology of this protein with the inner mitochondrial membrane transporters suggested a domain structure in which the N- and C-termini of the protein are located on the mitochondrial intermembrane-facing surface, ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi062191+
更新日期:2007-02-13 00:00:00
abstract::One way to improve the economic feasibility of biomass conversion is to enhance the catalytic efficiency of cellulases through protein engineering. This requires that high-resolution structures of cellulases be available. Here we present the structure of E2cd, the catalytic domain of the thermophilic endocellulase E2 ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00089a006
更新日期:1993-09-28 00:00:00
abstract::Hydroxyproline-rich glycoproteins (HRGPs) are the major proteinaceous components of higher plant walls and the predominant components of the cell wall of the green alga Chlamydomonas reinhardtii. The GP1 protein, an HRGP of the C. reinhardtii wall, is shown to adopt a polyproline II helical configuration and to carry ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0023605
更新日期:2001-03-06 00:00:00
abstract::Rhodanese bound to bacterial ribosomes as peptidyl-tRNA can be folded into an enzymatically active conformation by generating C-terminal extensions of the wild-type enzyme. Rhodanese was synthesized by coupled transcription/translation in a cell-free Escherichia coli system from plasmids containing the coding sequence...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00044a003
更新日期:1995-11-07 00:00:00
abstract::The solution structures of peptide antigens from the receptor binding domains of Pseudomonas aeruginosa strains PAO and KB7 have been determined using two-dimensional 1H NMR techniques. Ensembles of solution conformations for the trans forms of these 17-residue disulfide-bridged peptides have been generated using a si...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00050a005
更新日期:1995-12-19 00:00:00
abstract::G protein alpha subunits mediate activation of signaling pathways through G protein-coupled receptors (GPCR) by virtue of GTP-dependent conformational rearrangements. It is known that regions of disorder in crystal structures can be indicative of conformational flexibility within a molecule, and there are several such...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0255726
更新日期:2002-08-06 00:00:00
abstract::Type II polyketides are a class of natural products that include pharmaceutically important aromatic compounds such as the antibiotic tetracycline and antitumor compound doxorubicin. The type II polyketide synthase (PKS) is a complex consisting of 5-10 standalone domains homologous to fatty acid synthase (FAS). Polyke...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi7016427
更新日期:2008-02-19 00:00:00
abstract::Ribonucleotide reductase (RDPR) from Escherichia coli is composed of two subunits, R1 and R2, both of which are required to catalyze the conversion of nucleotides to deoxynucleotides. This reduction process is accompanied by oxidation of two cysteines within the active site to a disulfide. One of these putative active...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00155a030
更新日期:1992-10-13 00:00:00