Membrane permeabilization induced by cytolytic delta-endotoxin CytA from Bacillus thuringiensis var. israelensis.

abstract：:Adduct-induced DNA damage can affect transcription efficiency and DNA replication and repair. We previously investigated the effects of the 3'-next flanking base (G*CT vs G*CA; G*, FABP, N-(2'-deoxyguanosin-8-yl)-4'-fluoro-4-aminobiphenyl; FAF, N-(2'-deoxyguanosin-8-yl)-7-fluoro-2-aminofluorene) on the conformation of...

journal_title：Biochemistry

authors： Jain V,Vaidyanathan VG,Patnaik S,Gopal S,Cho BP

更新日期：2014-06-24 00:00:00

abstract：:Cyanide inhibits the CO oxidation activity of carbon monoxide dehydrogenase from Clostridium thermoaceticum by binding tightly to the form of the C-cluster yielding the gav = 1.82 signal (the C1.82 form). CN- dissociates and the enzyme reactivates upon addition of CO, CO2 plus dithionite, or CS2 plus dithionite. Dithi...

journal_title：Biochemistry

authors： Anderson ME,Lindahl PA

更新日期：1994-07-26 00:00:00

abstract：:To determine the active site cysteine residue in aldehyde dehydrogenase, we mutated amino acid residues 49, 162, and 302 of recombinantly expressed rat liver mitochondrial (class 2) aldehyde dehydrogenase. The C49A and C162A mutants were fully active tetrameric enzymes, although the C162A mutant was found to be highly...

journal_title：Biochemistry

authors： Farrés J,Wang TT,Cunningham SJ,Weiner H

更新日期：1995-02-28 00:00:00

abstract：:31P NMR spectroscopy of butyrylcholinesterase (BChE), acetylcholinesterase (AChE), and chymotrypsin (Cht) inhibited by pinacolyl methylphosphonofluoridate (soman), methylphosphonodifluoridate (MPDF), and diisopropyl phosphorofluoridate (DFP) allowed direct observation of the OP-linked moiety of aged (nonreactivatable)...

journal_title：Biochemistry

authors： Segall Y,Waysbort D,Barak D,Ariel N,Doctor BP,Grunwald J,Ashani Y

更新日期：1993-12-14 00:00:00

abstract：:Thermostable direct hemolysin (TDH) of Vibrio parahemolyticus is a membrane-damaging pore-forming toxin with potent cytolytic/cytotoxic activity. TDH exists as a tetramer consisting of protomers with a core β-sandwich domain, flanked by an 11-amino acid long N-terminal region (NTR). This NTR could not be modeled in th...

journal_title：Biochemistry

authors： Kundu N,Verma P,Kumar A,Dhar V,Dutta S,Chattopadhyay K

更新日期：2020-02-04 00:00:00

abstract：:The nuclear transport of the chromatin remodeling protein nucleoplasmin and chromatin building histones is mediated by importins. Nucleoplasmin (NP) contains a classical bipartite nuclear localization signal (NLS) that is recognized by the importin α/β heterodimer, while histones present multiple NLS-like motifs that ...

journal_title：Biochemistry

authors： Arregi I,Falces J,Bañuelos S,Urbaneja MA,Taneva SG

更新日期：2011-08-23 00:00:00

abstract：:Human alpha-lactalbumin (alpha-LA), a 123-residue calcium-binding protein, has been studied using (15)N NMR relaxation methods in order to characterize backbone dynamics of the native state at the level of individual residues. Relaxation data were collected at three magnetic field strengths and analyzed using the mode...

journal_title：Biochemistry

authors： Bruylants G,Redfield C

更新日期：2009-05-19 00:00:00

abstract：:Magainin and PGLa are 23- and 21-residue peptides isolated from the skin of the African clawed frog Xenopus laevis. They protect the frog from infection and exhibit a broad-spectrum antimicrobial activity in vitro. The mechanism of this activity involves the interaction of magainin with microbial membranes. We have me...

journal_title：Biochemistry

authors： Williams RW,Starman R,Taylor KM,Gable K,Beeler T,Zasloff M,Covell D

更新日期：1990-05-08 00:00:00

abstract：:PII proteins are sensors of alpha-ketoglutarate and adenylylate energy charge that regulate signal transduction proteins, metabolic enzymes, and permeases involved in nitrogen assimilation. Here, purified Escherichia coli PII and two of its receptors, ATase and NRII, were used to study the mechanisms of sensation by P...

journal_title：Biochemistry

authors： Jiang P,Ninfa AJ

更新日期：2009-12-08 00:00:00

abstract：:Electronic absorption and resonance Raman spectra, together with rates of arsenolysis, are used to show that the acylated subunits of the rabbit furylacryloylglyceraldehyde-3-phosphate dehydrogenase (FA-rabbit enzyme), but not the FA-sturgeon enzyme, exist as a mixed population of at least two forms. One form of the F...

journal_title：Biochemistry

authors： Storer AC,Phelps DJ,Carey PR

更新日期：1981-06-09 00:00:00

abstract：:The crystal structures of the guanosine 5'-monophosphate (GMP) and inosine 5'-monophosphate (IMP) complexes of Toxoplasma gondii hypoxanthine-guanine phosphoribosyltransferase (HGPRT) have been determined at 1.65 and 1.90 A resolution. These complexes, which crystallize in space groups P2(1) (a = 65.45 A, b = 90.84 A,...

journal_title：Biochemistry

authors： Héroux A,White EL,Ross LJ,Borhani DW

更新日期：1999-11-02 00:00:00

abstract：:The pyridoxal 5'-phosphate (PLP)-dependent cystathionine beta-lyase (CBL) was previously found to be inhibited by the natural toxins rhizobitoxine and l-aminoethoxyvinylglycine (AVG). The present study characterizes the interaction of Escherichia coli CBL with AVG and methoxyvinylglycine (MVG) by a combination of kine...

journal_title：Biochemistry

authors： Clausen T,Huber R,Messerschmidt A,Pohlenz HD,Laber B

更新日期：1997-10-14 00:00:00

abstract：:The extent of nativeness of the stable conformation of the thermolysin fragment containing the carboxyl-terminal third of the protein (from residues 206 to 316, denoted fragment FII) was examined by its immunogenic and antigenic characteristics. Antisera elicited in rabbits by either intact thermolysin or fragment FII...

journal_title：Biochemistry

authors： Vita C,Fontana A,Chaiken IM

更新日期：1982-04-27 00:00:00

abstract：:Colicin E3 is a 60 kDa, multidomain protein antibiotic that targets its ribonuclease activity to an essential region of the 16S ribosomal RNA of Escherichia coli. To prevent suicide of the producing cell, synthesis of the toxin is accompanied by the production of a 10 kDa immunity protein (Im3) that binds strongly to ...

journal_title：Biochemistry

authors： Walker D,Moore GR,James R,Kleanthous C

更新日期：2003-04-15 00:00:00

abstract：:A double mutant of the single-domain protein barstar having a single tryptophan (W53) was made by mutating the remaining two tryptophans (W38 and W44) into phenylalanines. W53 is buried in the core of barstar. Time-resolved fluorescence of the mutant barstar (W38FW44F) showed that W53 has a single fluorescence lifetim...

journal_title：Biochemistry

authors： Swaminathan R,Nath U,Udgaonkar JB,Periasamy N,Krishnamoorthy G

更新日期：1996-07-16 00:00:00

abstract：:A variant of the Tetrahymena ribozyme that efficiently cleaves single-stranded DNA under simulated physiological conditions [Tsang, J., & Joyce, G. F. (1994) Biochemistry 33, 5966-5973] was evaluated as a potential therapeutic agent on the basis of its ability to cleave synthetic oligonucleotide substrates correspondi...

journal_title：Biochemistry

authors： Raillard SA,Joyce GF

更新日期：1996-09-10 00:00:00

abstract：:Transcription of the human nonmuscle myosin II heavy chain-A (NMHC-A) gene is regulated via multiple elements located in intron 1, including element F which contains an E-box. In this study we have identified and characterized the factors that are capable of binding to element F. Yeast one-hybrid screening using eleme...

journal_title：Biochemistry

authors： Chung MC,Kim HK,Kawamoto S

更新日期：2001-07-31 00:00:00

abstract：:Escherichia coli PurK, a dimeric N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) synthetase, catalyzes the conversion of 5-aminoimidazole ribonucleotide (AIR), ATP, and bicarbonate to N5-CAIR, ADP, and Pi. Crystallization of both a sulfate-liganded and the MgADP-liganded E. coli PurK has resulted in structures at 2....

journal_title：Biochemistry

authors： Thoden JB,Kappock TJ,Stubbe J,Holden HM

更新日期：1999-11-23 00:00:00

abstract：:As a prerequisite to mammalian fertilization, the sperm acrosomal vesicle fuses with the plasma membrane and the acrosome contents are exocytosed. Induction occurs through engagement of the sperm receptors by multiple sugar residues. Multivalent polymers displaying mannose, fucose, or GlcNAc are effective synthetic in...

journal_title：Biochemistry

authors： Huang H,Rodolis MT,Bhatia SR,Sampson NS

更新日期：2017-06-06 00:00:00

abstract：:We have employed a combination of gel retardation, protein-DNA cross-linking, and protein-protein cross-linking techniques to further examine the 2,3,7,8-tetrachlorodibenzo-p- dioxin-(TCDD-) dependent changes in the Ah receptor that result in a DNA-binding conformation. Gel retardation analysis of DNA-Sepharose chroma...

journal_title：Biochemistry

authors： Gasiewicz TA,Elferink CJ,Henry EC

更新日期：1991-03-19 00:00:00

abstract：:Covalent labeling of the canine renal parathyroid hormone receptor with [125I]bPTH(1-34) reveals several major binding components that display characteristics consistent with a physiologically relevant adenylate cyclase linked receptor. Through the use of the specific glycosidases neuraminidase and endoglycosidase F a...

journal_title：Biochemistry

authors： Karpf DB,Arnaud CD,King K,Bambino T,Winer J,Nyiredy K,Nissenson RA

更新日期：1987-12-01 00:00:00

abstract：:alpha5beta1 integrin can occupy several distinct conformational states which support different strengths of binding to fibronectin [García, A. J., et al. (1998) J. Biol. Chem. 273, 34710-34715]. Using a model system in which specific activating monoclonal antibodies were used to achieve uniform activated states, the b...

journal_title：Biochemistry

authors： García AJ,Schwarzbauer JE,Boettiger D

更新日期：2002-07-23 00:00:00

abstract：:The functional role of the Ca (2+) ion in the oxygen-evolving complex of photosystem II is not yet clear. Current models explain why the redox cycle of the complex would be interrupted after the S 3 state without Ca (2+), but the literature shows that it is interrupted after the S 2 state. Reinterpretation of the lite...

journal_title：Biochemistry

authors： Miqyass M,Marosvölgyi MA,Nagel Z,Yocum CF,van Gorkom HJ

更新日期：2008-07-29 00:00:00

abstract：:Triple helix-forming oligonucleotides (TFOs) bind with high affinity and specificity to homopurine-homopyrimidine sequences in DNA and have been shown to inhibit transcription of target genes in various experimental systems. In the present study, we evaluated the ability of 3'-amino-modified phosphodiester TFOs direct...

journal_title：Biochemistry

authors： Catapano CV,McGuffie EM,Pacheco D,Carbone GM

更新日期：2000-05-02 00:00:00

abstract：:Amyloid beta peptide (A beta) is released into the media of a variety of cells in culture during normal metabolism. The discovery of several missense mutations within or flanking the A beta region of the beta amyloid precursor protein (beta APP) in familial Alzheimer's disease provides strong evidence for a role of al...

journal_title：Biochemistry

authors： Querfurth HW,Selkoe DJ

更新日期：1994-04-19 00:00:00

abstract：:The solution behavior of bilirubin ditaurate (BDT), the first naturally occurring conjugated bile pigment to be physically and chemically characterized, was assessed in aqueous solution and in monomeric and micellar solutions of common taurine-conjugated bile salts (BS). Analytical ultracentrifugation revealed that BD...

journal_title：Biochemistry

authors： Neubrand MW,Carey MC,Laue TM

更新日期：2015-02-24 00:00:00

abstract：:An RNA that replicates with core RNA polymerase from E. coli and the substrates ATP, CTP, ITP, and UTP, was selected from a random poly(A,U,I,C) library and named EcorpI. Another replicating RNA, EcorpG, was obtained by template-free incubation of holo RNA polymerase and the substrates ATP, CTP, GTP, and UTP. Both RNA...

journal_title：Biochemistry

authors： Wettich A,Biebricher CK

更新日期：2001-03-20 00:00:00

abstract：:PKC phosphorylation regulates PITX2 DNA binding and transcriptional activity. Mutation of individual PKC sites demonstrates the functional regulation of PITX2 through phosphorylation. Immunoprecipitation of PITX2 and a PITX2 PKC mutant protein reveal specific in vivo phosphorylation by PKC in transfected cells. The tr...

journal_title：Biochemistry

authors： Espinoza HM,Ganga M,Vadlamudi U,Martin DM,Brooks BP,Semina EV,Murray JC,Amendt BA

更新日期：2005-03-15 00:00:00

abstract：:The catalytic activity of protein-tyrosine phosphatases (PTPs) is mediated by a cysteine side chain which carries out a nucleophilic attack initiating the phosphate cleavage. Experimentally, it has been observed that the active site cysteine has a remarkably low pKa. In the present study, we have investigated the orig...

journal_title：Biochemistry

authors： Peters GH,Frimurer TM,Olsen OH

更新日期：1998-04-21 00:00:00

abstract：:Microtubules, ordinarily cold-labile structures, are made entirely resistant to cold temperature by the presence of substoichiometric amounts of STOP (stable tubule only polypeptide), a microtubule-associated protein. We have produced a monoclonal antibody which specifically recognizes a 145-kDa protein previously imp...

journal_title：Biochemistry

authors： Pirollet F,Rauch CT,Job D,Margolis RL

更新日期：1989-01-24 00:00:00