Abstract：
:Human alpha-lactalbumin (alpha-LA), a 123-residue calcium-binding protein, has been studied using (15)N NMR relaxation methods in order to characterize backbone dynamics of the native state at the level of individual residues. Relaxation data were collected at three magnetic field strengths and analyzed using the model-free formalism of Lipari and Szabo. The order parameters derived from this analysis are generally high, indicating a rigid backbone. A total of 46 residues required an exchange contribution to T(2); 43 of these residues are located in the alpha-domain of the protein. The largest exchange contributions are observed in the A-, B-, D-, and C-terminal 3(10)-helices of the alpha-domain; these residues have been shown previously to form a highly stable core in the alpha-LA molten globule. The observed exchange broadening, along with previous hydrogen/deuterium amide exchange data, suggests that this part of the alpha-domain may undergo a local structural transition between the well-ordered native structure and a less-ordered molten-globule-like structure.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Bruylants G,Redfield Cdoi
10.1021/bi900023msubject
Has Abstractpub_date
2009-05-19 00:00:00pages
4031-9issue
19eissn
0006-2960issn
1520-4995journal_volume
48pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The purified PBS2 phage-coded inhibitor of uracil-DNA glycosylase (Ura-DNA glycosylase) from Bacillus subtilis has been tested for its ability to inhibit this enzyme isolated from other prokaryotic and from eukaryotic sources. In addition, the inhibitor has been assayed for its effect on DNA glycosylases specific for ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00524a027
更新日期：1981-10-13 00:00:00
abstract：:Stopped-flow measurements have been employed to study the kinetics of the conformational changes in TetR (B) induced by tetracycline binding with and without Mg(2+) ions. Result of stopped-flow fluorometry measurements at pH 8.0 indicate conformational changes in the helix-turn-helix motif in the N-terminal domain and...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi048548w
更新日期：2005-01-25 00:00:00
abstract：:The present paper reports a study on the equilibria and kinetics of the acid-alkaline transition and the azide binding reaction by ferric Aplysia myoglobin. A single completely reversible spectrophotometric titration curve is found over the pH range from similar to 5 to similar to 9, with an apparent pK equals to 7.5 ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00679a006
更新日期：1975-04-22 00:00:00
abstract：:We have studied the pathway and kinetics of refolding of recombinant human brain-derived neurotrophic factor (BDNF), which is a very tightly-associated dimer in its native state. When BDNF unfolded in 6 M guanidine hydrochloride is diluted 20-fold into phosphate-buffered saline, a partially folded intermediate is rapi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00091a036
更新日期：1993-10-12 00:00:00
abstract：:The Y70F mutant of aspartate aminotransferase has reduced affinity for coenzymes compared to the wild type. The equilibrium dissociation constants for pyridoxamine phosphate (PMP) holoenzymes, KPMPdiss, were determined from the association and dissociation rate constants to be 1.3 nM and 30 nM for the wild type and mu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00244a014
更新日期：1991-07-30 00:00:00
abstract：:Structurally diverse small molecules, including 5-(2-benzofuryl)-4-phenyl-1,2,4-triazole-3-thiol (BETT), have been identified via high-throughput screening as activators of caspase-3 in HeLa cell extracts. However, little is known about their mechanism of action. In this study, we investigate how BETT regulates prothy...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9022329
更新日期：2010-03-09 00:00:00
abstract：:The iron-sulfur flavoenzyme adenosine-5'-phosphosulfate (APS) reductase catalyzes a key reaction of the global sulfur cycle by reversibly transforming APS to sulfite and AMP. The structures of the dissimilatory enzyme from Archaeoglobus fulgidus in the reduced state (FAD(red)) and in the sulfite adduct state (FAD-sulf...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0521689
更新日期：2006-03-07 00:00:00
abstract：:The human double-stranded RNA- (dsRNA) activated protein kinase (PKR) has a dsRNA-binding domain (dsRBD) that contains two tandem copies of the dsRNA-binding motif (dsRBM). The minimal-length polypeptide required to bind dsRNA contains both dsRBMs, as determined by mobility-shift and filter-binding assays. Mobility-sh...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9607259
更新日期：1996-08-06 00:00:00
abstract：:Peptides corresponding to portions of curaremimetic neurotoxin loop 2 and to a structurally similar segment of rabies virus glycoprotein were synthetically modified in order to gain information on structure-function relationships of neurotoxin loop 2 interactions with the acetylcholine receptor. Binding of synthetic p...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00109a020
更新日期：1991-11-12 00:00:00
abstract：:The Stk1/Stp1 and GraSR signal-transduction pathways are two distinct pathways in Staphylococcus aureus that rely on a reversible phosphorylation process in transducing external stimuli intracellularly. Stk1/Stp1 is an eukaryote-like Ser/Thr kinase phosphatase pair involved in purine biosynthesis, cell-wall metabolism...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi401177n
更新日期：2013-11-12 00:00:00
abstract：:Direct chemical titrations of reduced, purified cytochrome aa3 were monitored at 604 nm. Anaerobic oxidation by potassium ferricyanide or 1,1'-bis(hydroxymethyl)-ferricinium ion was compared with the natural substrate, molecular oxygen. The four electrons from reduced cytochrome aa3 were donated to one oxygen molecule...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00626a014
更新日期：1977-04-05 00:00:00
abstract：:Photosystem II, the multisubunit protein complex that oxidizes water to O2, requires the inorganic cofactors Ca2+ and Cl- to exhibit optimal activity. Chloride can be replaced functionally by a small number of anionic cofactors (Br-, NO3-, NO2-, I-), but among these anions, only Br- is capable of restoring rates of ox...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi982295n
更新日期：1999-03-23 00:00:00
abstract：:Three activities on the pathway of purine biosynthesis de novo in chicken liver, namely, glycinamide ribonucleotide synthetase, glycinamide ribonucleotide transformylase, and aminoimidazole ribonucleotide synthetase, have been found to reside on the same polypeptide chain. Three diverse purification schemes, utilizing...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00346a006
更新日期：1985-12-03 00:00:00
abstract：:DNA gyrase and topoisomerase IV (Topo IV) are cellular targets of quinolone antibacterial drugs. The Ser-80 and the Glu-84 of the ParC subunit have been identified as mutational hotspots for quinolone resistance. Mutant Topo IV proteins containing a quinolone resistance-conferring mutation have been constructed, and t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi026352v
更新日期：2002-10-01 00:00:00
abstract：:Streptococcus pneumoniae 5'-methylthioadenosine/S-adenosylhomocysteine hydrolase (MTAN) catalyzes the hydrolytic deadenylation of its substrates to form adenine and 5-methylthioribose or S-ribosylhomocysteine (SRH). MTAN is not found in mammals but is involved in bacterial quorum sensing. MTAN gene disruption affects ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi061184i
更新日期：2006-10-31 00:00:00
abstract：:Serine palmitoyltransferase (EC 2.3.1.50) catalyzes the condensation of L-serine and palmitoyl-CoA to yield 3-ketosphinganine in the first unique reaction of long-chain (sphingoid) base biosynthesis. The kinetic effects of changing the extracellular concentrations of the precursors for this pathway were studied with L...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00401a051
更新日期：1988-01-12 00:00:00
abstract：:A major biochemical goal is the ability to mimic nature in engineering highly specific protein-protein interactions (PPIs). We previously devised a computational interactome screen to identify eight peptides that form four heterospecific dimers despite 32 potential off-targets. To expand the speed and utility of our a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b00047
更新日期：2017-03-21 00:00:00
abstract：:Detailed analysis of factors governing high affinity antibody-antigen interactions yields important insight into molecular recognition and facilitates the design of functional antibody libraries. Here we describe comprehensive mutagenesis of the light chain complementarity determining regions (CDRs) of HIV-1 antibody ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi100293q
更新日期：2010-07-06 00:00:00
abstract：:Several enzymatic and chemical reagents were used to probe the secondary structure of Saccharomyces cerevisiae nuclear RNase P RNA in the presence and absence of its protein components. Double-stranded regions were detected with RNase V1 and single-stranded regions with RNase ONE (Escherichia coli RNase I). Nucleotide...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00173a022
更新日期：1994-02-22 00:00:00
abstract：:We have initiated a broad-based program aimed at understanding the molecular basis of fluorine specificity in enzymatic systems, and in this context, we report crystallographic and biochemical studies on a fluoroacetyl-coenzyme A (CoA) specific thioesterase (FlK) from Streptomyces cattleya. Our data establish that FlK...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi101102u
更新日期：2010-11-02 00:00:00
abstract：:Deposition of misfolded alpha-synuclein is a critical factor in several neurodegenerative disorders. Filamentous alpha-synuclein is the major component of Lewy bodies and Lewy neurites, the intracellular inclusions in the dopaminergic neurons of the substantia nigra, which are considered the pathological hallmark of P...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi034938r
更新日期：2004-03-23 00:00:00
abstract：:Electronic absorption and resonance Raman spectra, together with rates of arsenolysis, are used to show that the acylated subunits of the rabbit furylacryloylglyceraldehyde-3-phosphate dehydrogenase (FA-rabbit enzyme), but not the FA-sturgeon enzyme, exist as a mixed population of at least two forms. One form of the F...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00515a023
更新日期：1981-06-09 00:00:00
abstract：:The first positive evidence for the utilization of a direct C-6' ' oxidation/reduction mechanism by ADP-l-glycero-d-manno-heptose 6-epimerase is reported here. The epimerase (HldD or AGME, formerly RfaD) operates in the biosynthetic pathway of l-glycero-d-manno-heptose, which is a conserved sugar in the core region of...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi050106c
更新日期：2005-04-19 00:00:00
abstract：:Messenger RNA degradation is an important component of overall gene expression. During the final step of eukaryotic mRNA degradation, exoribonuclease 1 (Xrn1) carries out 5' → 3' processive, hydrolytic degradation of RNA molecules using divalent metal ion catalysis. To initiate studies of the 5' → 3' RNA decay machine...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.9b01035
更新日期：2020-04-21 00:00:00
abstract：:Site-directed spin labeling (SDSL), the site-specific incorporation of nitroxide spin-labels into a protein, has allowed us to investigate ligand-induced conformational changes in the ligand-binding domain of human estrogen receptor alpha (hERalpha-LBD). EPR (electron paramagnetic resonance) spectroscopy of the nitrox...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi035566p
更新日期：2004-02-24 00:00:00
abstract：:The kinetic and equilibrium CO binding properties of hemoglobin (Hb) M Iwate (alpha2 87 His leads to Tyr beta2) have been investigated. The results show that the alpha(Met) beta2 (CO) tetramer of this protein has a low affinity for CO, as indicated by the stopped-flow and flash-photolysis kinetic, as well as the CO bi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00669a022
更新日期：1976-11-30 00:00:00
abstract：:Glutamate semialdehyde aminomutase is a recognized target for selective herbicides and antibacterial agents because it provides the aminolevulinate from which tetrapyrroles are synthesized in plants and bacteria but not in animals. The reactions of the enzyme with R- and S-enantiomers of a novel compound, diaminopropy...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi992307k
更新日期：2000-03-21 00:00:00
abstract：:Various agents are able to stimulate the EGF receptor protein tyrosine kinase in the absence of ligand binding. To characterize their mechanism of action, we investigated their effects on the kinase activity of the intracellular domain of the EGF receptor (EGFR-IC). EGFR-IC (67 kDa) lacking the extracellular domain an...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00085a004
更新日期：1993-08-31 00:00:00
abstract：:Structural properties of heparan sulfate preparations from hog mucosa and beef lung sources were obtained by application of Smith degradation and nitrous acid reactions. Products formed by these reactions indicated that most of the iduronic acid present in these mucopolysaccharides is ester sulfated, whereas N-sulfate...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00629a014
更新日期：1977-05-17 00:00:00
abstract：:Aflatoxin B1 (AFB1) exo-8,9-epoxide, the reactive product of the hepatocarcinogen AFB1, is stable in aprotic solvents but hydrolyzes rapidly in H2O at 25 degrees C and pH 7 (t1/2 = 1 s). However, it is also known that some glutathione (GSH) transferases can conjugate the epoxide with GSH to give the adduct in high yie...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi962537o
更新日期：1997-03-18 00:00:00
